Search

Effects of substituting chemical nitrogen fertilizer with organic nitrogen fertilizer on greenhouse spinach yield, quality, soil fertility and rhizosphere bacterial community were studied. The experiment adopted an equal nitrogen design with six treatments, i. e., 0 (CF), 20% (OM20), 40% (OM40), 60% (OM60), 80% (OM80), and 100% (OM100) of chemical nitrogen being replaced respectively. The results showed that 20% of chemical nitrogen replacement with organic fertilizer was most conducive to improving the yield and quality of spinach, with fresh and dry weight, vitamin C, and soluble sugar significantly higher than that of CF. In all treatments, adding organic fertilizer could significantly increase the soil pH, organic matter, nitrogen, available phosphorus, and available potassium content, and significantly reduce the bulk density. When the proportion of organic nitrogen fertilizers was 20%-80%, the content of alkaline nitrogen was significantly lower than CF. Applying organic fertilizer could significantly increase the α diversity of soil bacteria and reduce β diversity. Spinach organic acid content and soil alkaline nitrogen content significantly contributed to β diversity, which was 12. 80% and -4. 71% respectively. Application of organic fertilizer can improve the relative abundance of beneficial bacteria such as aerobic and stress tolerant, but it might also increase the relative abundance of potential pathogenic bacteria. In summary, the replacement of chemical nitrogen fertilizer with organic nitrogen fertilizers can improve the quality of spinach and improve soil fertility and resistance. [ABSTRACT FROM AUTHOR]

Fe3O4 Nanospheres with good dispersibility were synthesized by hydrothermal method, and mesoporous silica (MSN) was coated on their outer layer. Chitosan (CS) and hyaluronic acid (HA) were further modified to form highly dispersed nanospheres Fe3O4@SiO2@MSN@CS@HA. The structure, morphology, particle size, and dispersibility of several nanospheres were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The outer layer content, specific surface area, and pore size were measured using a thermogravimetric analyzer (TGA) and a specific surface area analyzer (BET). The performance of the Fenton reaction was measured using a UV visible spectroscopy analyzer and its reaction rate was calculated. The results indicate that nanospheres have good dispersibility and drug loading ability, and the divalent iron ions(Fe2+) they contain can further realize the potential of chemodynamic therapy (CDT) for tumors. [ABSTRACT FROM AUTHOR]

Ischemic stroke is a common acute cerebrovascular disease in clinical practice, which poses a severe threat to human health. In recent years, with deepening understanding of ischemic stroke, significant progress has been made in the diagnosis and treatment. However, current treatments for ischemic stroke are partially limited due to extremely complex pathological mechanisms. Studies have shown that mitochondrial dysfunction plays an important role in the pathogenesis of ischemic stroke. Therefore, modulation of mitochondrial function through mitochondrial dynamics is essential to ameliorate the damage of cerebral ischemic neuronal cells. In this article, the molecular mechanism of mitochondrial dynamics and its role in ischemic stroke were reviewed, aiming to provide useful reference for the treatment of ischemic stroke. [ABSTRACT FROM AUTHOR]

BACKGROUND: Skeletal muscle insulin resistance is the key pathological link of type 2 diabetes. Static exercise can effectively improve skeletal muscle insulin resistance, but the mechanism remains unclear. OBJECTIVE: To explore the mechanism of static exercise on insulin resistance in the skeletal muscle of type 2 diabetic mice based on the phosphatidyl inositol 3-kinase (PI3K)/protein kinase B (AKT)/glucose transporter (GLUT4) signaling pathway. METHODS: After 1 week of adaptive feeding, 7 out of 40 C57BL/6 mice were randomly selected as blank group and fed common diet, while the other mice were fed high-fat diet and taken to prepare type 2 diabetes models through the low-dose streptozotocin intraperitoneal injection. Twenty-four mice were successfully modeled and they were randomly divided into model group (n=8), metformin group (n=8) and static exercise group (n=8), which continued to be fed high-fat diet. The metformin group was given 200 mg/kg metformin dissolved in normal saline (2 ml/kg) by gavage, once a day, for 6 weeks. The static exercise group was given normal saline daily by gavage and carried out static exercise, 30 minutes a day, 6 days per week. The model group was given the same dose of normal saline daily by gavage without exercise intervention. After the intervention, the fasting blood glucose of each group was detected, the intraperitoneal glucose tolerance test was performed, and the area under the glycemic curve was calculated. Glycosylated hemoglobin, serum insulin, insulin resistance index were detected by ELISA. Total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein were detected using biochemical methods. The mRNA expression levels of PI3K, AKT and GLUT4 in the gastrocnemius of mice were detected by real-time quantitative PCR. Morphological changes of the gastrocnemius were observed by hematoxylin-eosin staining, and the cross-sectional area of muscle fibers was calculated. RESULTS AND CONCLUSION: Compared with the blank group, fasting blood glucose, glycosylated hemoglobin, area under the glycemic curve, insulin resistance index, total cholesterol, triglyceride and low-density lipoprotein levels were significantly increased in the model group (P < 0.01, P < 0.05). Whereas, these indicators were significantly lower in the static exercise and metformin group than the model group (P < 0.01, P < 0.05). Compared with the blank group, serum insulin and high-density lipoprotein levels were significantly declined in the model group (P < 0.01) and the mRNA expression of PI3K, AKT and GLUT4 in the gastrocnemius of mice were also significantly reduced (P < 0.01). These indicators were significantly elevated in the metformin group and static exercise group compared with the blank group (P < 0.01). Compared with the blank group, the muscle fibers in the model group were disordered, and the muscle cells atrophied and the muscle fiber gap widened. The cross-sectional area of muscle fibers was significantly decreased in the model group compared with the blank group (P < 0.01). Compared with the model group, atrophy of the gastrocnemius fibers and muscle fiber space were improved in the static exercise group and the metformin group, and the cross-sectional area of muscle fiber was significantly increased in both groups (P < 0.01). These findings indicate that static resistance training may promote glucose uptake and utilization by up-regulating the expression of PI3K, AKT and GLUT4 mRNA in skeletal muscle tissue, thereby improving the morphology and function of skeletal muscle tissue, alleviating insulin resistance and regulating glucose homeostasis. [ABSTRACT FROM AUTHOR]

[Objective]To clarify the improvement effect of polyacrylamide (PAM) on sandy gravel beach in coastal difficult sites. [Method] Rhizophora stylosa was used as the research material to explore the effects of different PAM application rates (0. 25%, 0. 50%, 0. 75%, 1. 00%, 1. 25%, respectively recorded as P1, P2, P3, P4, P5 ) mixed with silt and compound biological inoculants on plant growth and soil nutrients through tidal-simulation test. [Result]Compared with CK treatment, in different PAM application rates treatments, the tree heights and number of leaves increased to 18. 75%-48. 82% and 5. 0%-55. 0%, respectively;the ground diameters significantly increased to 43. 16% -71. 43%;the roots and stems biomass increased to 16. 41%-87. 26% and 14. 91%-72. 75%, respectively;the leaves biomass significantly increased to 64. 36%-156. 93%, and the root-shoot ratio significantly decreased to 15. 62%-26. 98%. The application of PAM had a greater impact on soil organic matter, the total and available nutrients of soil phosphorus and potassium, and the soil organic matter, total phosphorus and total potassium significantly increased to 623. 47%-794. 78%, 114. 49%-142. 03% and 145. 90%-167. 24%, respectively;the available phosphorus and available potassium significantly increased to 146. 55%-268. 12%, 24. 69%-61. 74%, respectively. [Conclusion]The mixed application of PAM with silt and compound biological inoculants can promote the growth of Rhizophora stylosa seedlings and improve soil nutrients. It can be used as soil conditioner for sandy gravel beach in coastal difficult sites and the recommended application rate of PAM is 0. 75%. [ABSTRACT FROM AUTHOR]

Household electric vehicle charging in residential areas has a strong centrality. Large-scale electric vehicle charging load causes large peak valley load difference and other problems in the distribution network system. This study proposes a user charging selection control strategy based on Multi Objective Particle Swarm Optimization (MPSO) algorithm. Through the analysis and prediction of electric vehicle charging load, a multi-objective optimization model is established with the minimum variance of the total system load and scheduling cost as the objective function. Meanwhile, considering the constraints of electric vehicle battery and system power, the MPSO algorithm is used to solve the optimal initial charging time of electric vehicles. The simulation results show that compared with unordered charging of EVs in residential areas, the EV charging strategy proposed in this study can effectively reduce load peak and dispatch cost. [ABSTRACT FROM AUTHOR]

Twin reversed arterial perfusion sequence (TRAPS) is a rare and severe complication of monochorionic twin pregnancies, due to abnormal placental-fetal vascular anastomosis, which is characterized by absence of heart or loss of normal cardiac structure in one twin. We reported a case of triplet pregnancy complicated by TRAPS after assisted reproductive technology. Two cleavage-stage frozen embryos were transferred, one of which developed into monochorionic twin pregnology. The TRAPS was diagnosed by ultrasound at 8 +5 weeks. However, due to personal factors, the patient did not intervene in a timely manner and the pump twin died. Another single fetus developed normally until full term delivery. If the treatment is needed, as for the technique of choice to interrupt the vascular supply to the acardiac twin, the radiofrequency ablation of the intrafetal vessel is usually the preferred approach. However, the best timing of intervention is still debated. [ABSTRACT FROM AUTHOR]

Objective To explore the effects and mechanism of methylglyoxal (MGO) in human brain microvascular endothelial cell line (hCMEC/D3). Methods The hCMEC/D3 cells were incubated with MGO (0.25, 0.50, 0.75, 1.00 and 1.25 mmol/L) for 24 h. Cell viability was detected by CCK-8 assay. Cellular LDH and SOD activities were determined by commercially available kits. The change of mitochondrion membrane potential was assessed by JC-1 probe. MitoSOX probe was used to observe mitochondrial reactive oxygen species (mROS) release. Results Compared with control, cell viability of hCMEC/D3 treated with MGO decreased in a concentration-dependent manner (P＜0.01). Increased LDH activity and decreased SOD activity were observed with MGO exposure (P＜0.01). The decrease of mitochondrion membrane potential and the excessive increase of mROS were induced after MGO exposure for 12 h, which were detected by JC-1 or MitoSOX probe. Conclusions MGO reduces cell viability and mitochondria membrane potential in hCMEC/D3 cells with excessive mROS generation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Because of the poor regeneration ability of chondrocytes after damage, the use of stem cells as a basic treatment for repairing cartilage damage has become a popular treatment method. OBJECTIVE: To observe the effect of psoralen combined with transforming growth factor β1 on the differentiation of rat bone marrow mesenchymal stem cells into chondrocytes. METHODS: Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley rats and cultured by marrow adherent method. The effective concentration to promote bone marrow mesenchymal stem cells proliferation was selected by CCK-8 assay. Bone marrow mesenchymal stem cells were assigned to four groups. In the normal control group, cells were treated with low-sugar DMEM with 10% fetal bovine serum and 100 U/mL double antibodies. In the psoralen group, cells were treated with low-sugar DMEM with 10% fetal bovine serum, 100 U/mL double antibodies, 10-7 mol/L dexamethasone, 50 mg/L vitamin C, and 10 μmol/L psoralen. In the combination group, cells were treated with low-sugar DMEM with 10% fetal bovine serum, 100 U/mL double antibodies, 10 μg/L transforming growth factor β1, 10-7 mol/L dexamethasone, 50 mg/L vitamin C, and 10 μmol/L psoralen. In the positive control group, cells were treated with low-sugar DMEM with 10% fetal bovine serum, 100 U/mL double antibodies, 10 μg/L transforming growth factor β1, 10-7 mol/L dexamethasone, and 50 mg/L vitamin C. After 21 days, the expression of type ll collagen was detected by immunocytochemistry. RT-PCR and western blot assay were utilized to detect the expression levels of differentiation marker SOX-9 and type ll collagen mRNA and protein. RESULTS AND CONCLUSION: (1) Immunocytochemical staining showed that no positive cells were found in the normal control group, while type ll collagen positive cells were found in the other three groups. (2) Compared with the normal control group, the expression of SOX9 and type II collagen mRNA and protein increased significantly in the psoralen group, combination group and positive control group (P < 0.05), and the combination group had the best effect (P < 0.01), and it was significantly better than that in the positive control group and the psoralen group (P < 0.05). (3) The results show that within a certain concentration range, psoralen can promote the proliferation of bone marrow mesenchymal stem cells. Psoralen can induce bone marrow mesenchymal stem cells to differentiate into cartilage, and its combined use with transforming growth factor β1 has a synergistic promoting effect. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of chromodomain helicase DNA-binding protein 4 (CHD4) on the proliferation and migration of cervical cancer HeLa cells by regulating telomeres function. Methods CHD4-depleted HeLa cell lines were constructed by lentivirus infection. The mRNA and protein expression levels of CHD4 were detected by realtime fluorescence quantitative PCR (qPCR) and Western blot assay. Cervical cancer HeLa cells were divided into the control group, the shCDH4-1 group and the shCHD4-2 group. The effect of CHD4 on cell proliferation of HeLa cells was detected by CCK-8 assay. The colony formation assay was performed to detect the number of cell colonies. Scratch-healing assay was performed to detect cell migration. In vivo, the tumor formation experiment was used to observe the growth changes of xenograft in nude mice. Immunofluorescence-fluorescence in situ hybridization was performed to detect the co-localization of telomeres and CHD4 in HeLa cells, and the level of damage at telomeres in each group. Telomere damage was indicated by the co-localization of damage factor γH2AX with telomeres. Metaphase-telomere fluorescence in situ hybridization was performed to detect changes in telomere function in each group, and the increased proportion of chromosomes with telomere signal deletion (SFE) or multiple telomere signals (MTS) represented telomere dysfunction. Results HeLa cell lines with stable down-regulated CHD4 were successfully constructed after lentiviral infection (P＜0.05). Compared with the control group, the cell proliferation ability, colony formation ability, migration ability and tumor growth ability in vivo were significantly decreased in the shCHD4-1 group and the shCHD4-2 group (P＜0.05). Immunofluorescence-fluorescence in situ hybridization assay showed that CHD4 localized to telomeres of HeLa cells. Compared with the control group, the deletion of CHD4 was insufficient to cause DNA damage at telomeres (P＞0.05). However, the proportion of SFE chromosomes in HeLa cells increased significantly (P＜0.05). Conclusion CHD4 can promote the proliferation and migration of HeLa cells by regulating telomere function. [ABSTRACT FROM AUTHOR]

With the unavailability of all growth promoters except Chinese medicine herb in animal feed, the application of Chinese medicine herb in animal feed has gradually become a research hotspot. Adding proper amount of specific Chinese medicinal herb or extracts in aquatic animal feed could not only enhance the immunity of aquatic animals, but also improve the feed ingestion and growth rate, and decrease the lipid level of aquatic products. The paper reviews the feeding value of common Chinese medicine herb and their extracts in aquatic animal feed, which can not only promote the development and utilization of Chinese medicine herb resources, but also provide reference for the rational use of single and compound Chinese medicine herb in aquatic feed and healthy aquaculture of aquatic animals. [ABSTRACT FROM AUTHOR]

Through self-stable precipitation polymerization, styrene (St), maleic anhydride (MA), and glycidyl methacrylate (GMA) were copolymerized to obtain nanoparticles with regular morphology and uniform particle size. This one-pot method has no stabilizer and emulsifier, and requires no stirring. The post-treatment is simple, and the product can be obtained only by centrifugation. The effects of monomer feed ratio and solvent on the morphology and chemical composition of nanoparticles were studied by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The prepared nanoparticles can be used as emulsifiers to stabilize Pickering emulsion and immobilize lipase through epoxy groups. It was verified that lipase was anchored on the oil-water interface by immobilizing on the particles. The influence of the contact angle of particles and the solvent volume ratio of n-Heptane to water (RO/W) on the emulsion size was observed by fluorescence microscope. With the increase of contact angle and RO/W, the emulsion size decreases, which is also beneficial to the enzymatic reaction. The effects of nanoparticle mass content, lipase initial mass concentration, RO/W on the size of Pickering emulsion and subsequent enzyme catalyzed reaction were investigated. At the condition of n(GMA)∶n(St)∶n(MA) = 2∶1∶1, the mass content of nanoparticles 0.5%, the mass concentration of lipase 3 mg/mL, and RO/W 5∶5, the specific activity of immobilized enzyme was the highest, which was up to 7.5 times that of free enzyme, and it showed excellent reusability. [ABSTRACT FROM AUTHOR]

目的：分析输血不良反应的分布特点、影响因素、处置及药物治疗情况。方法：选取2021年9月-2023年 9月本院200例输血患者开展研究，依据有无不良反应，将其分为对照组180例（无不良反应），观察组20例（有不良 反应），分析影响因素和分布特点，探讨处置措施和药物疗法。结果：观察组20例患者中有13例出现发热寒战，有6 例出现过敏，有1例出现其他；1例为机采血小板，7例为血浆，12例为悬浮红细胞；20例患者中有1例为儿科，有2例 为骨科，有2例为肿瘤科，有4例为血液科，有4例为普外科，有6例为妇产科，有1例为其他；观察组有血液疾病、有 输血史、有过敏史和输血次数≥3次占比均明显高于对照组（P < 0.05）；多因素有血液疾病、输血史、过敏史和输血 次数（P < 0.05）。结论：输血不良反应主要有过敏反应和发热寒战等，影响因素较多，临床应积极采用适当处置措 施，采取适宜药物，提升输血安全。 [ABSTRACT FROM AUTHOR]

Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the oral and maxillofacial region with high incidence and poor prognosis. Cancer cells change their metabolism through glycolytic metabolic reprogramming to support their increased demand for ATP. Glycolytic reprogramming mediates the development of multisystem tumors, including oral squamous cell carcinoma, involving multiple signaling pathways and key factors. In this paper, the changes of glycolytic metabolism, key factors and potential clinical diagnostic and theraputic approches of OSCC are summarized, and the role of OSCC in oral squamous cell carcinoma is reviewed in order to provide new research directions and ideas. [ABSTRACT FROM AUTHOR]

To diagnose a sudden young pigeon co-infection of PPMV-1 and E. coli in a youngpigeon farm in Taiyuan, in this study, by clinical diagnosis and pathological autopsy of pathogenetic pigeons, disease tissue materials of pathogenetic pigeons were collected to conduct RT-PCR test, isolation and culture of specific-pathogen(SPF) chicken embryo virus and bacteria, biochemical identification, drug sensitivity test, and animal regression test for making comprehensive prevention and control measures and providing guidance for prevention and treatment of co-infection of PPMV-1 and E. coli. The results showed that the virus infecting pigeons in the pigeon farm was the pigeon paramyxovirus type 1(PPMV-1) and the isolated bacteria were Escherichia coli. Drug sensitivity test indicated that the isolated bacteria were highly sensitive to aminoglycosides. According to the diagnosis results, after co use of Newcastle disease IV vaccine and sensitive drug amikacin, the epidemic was controlled soon. The antibody detection found that the level of Newcastle disease antibody in breeding pigeons was uneven, which was a main reason for PPMV-1 infection. Animal regression test further identified the pathogenicity of PPMV-1 and E. coli, the morbidity and mortality of PPMV-1were 100% and 80%. But there was no obviously clinical symptoms in chicken, indicating that the virus had different pathogenicity for pigeons and chickens. The mortality of co-infection was higher than that of PPMV-1 or E. coli alone. It indicated that comprehensive control should be emphasized in young pigeons to prevent co-infection. [ABSTRACT FROM AUTHOR]

To comprehensively understand the effect of selenium(Se)supplementation on Codonopsis pilosula, effects of sodium selenite levels on Se accumulation, growth, physiology and quality were explored by using hydroponic experiments. The results were as follows:(1)Treatment with 0.2 mg·L-1 Se promoted leaf size, plant height and biomass of C. pilosula, while treatment with 10 mg·L-1 Se suppressed them.(2)Both increasing Se level and exposure time to C. pilosula seedlings could raise its Se contents in root and leaf, and Se was distributed in organs with an order of root>leaf>stem.(3)Treatment with 0.2 mg·L-1 Se increased photosynthetic pigment content and root activity, and reduced malondialdehyde, proline and hydrogen peroxide contents, while treatment with 10 mg·L-1 Se had opposite effects.(4)Treatment with 0.2 mg·L-1 Se had positive effects on accumulations of lobetyolin, polysaccharide, and soluble protein, while treatment with 10 mg·L-1 Se had negative effects on the accumulations of polysaccharide and soluble protein. In summary, sodium selenite has double effect on C. pilosula; application of appropriate Se is beneficial to its growth, plant physiology and quality, and 0.2 mg·L-1 Se level is the optimum. This study may improve us to understand the effect of selenite level on the biology effect of C. pilosula, which will be useful for the basic research and cultivation of Se-enriched C. pilosula. [ABSTRACT FROM AUTHOR]

The restoration and reconstruction of mangrove wetland ecosystem is one of the key research fields in ecological restoration in the coastal zone of South China. In order to reveal the physiological and ecological strategies to light conditions of mangrove species, the characteristics of leaf soluble protein contents and activities of antioxidant enzymes in seedlings of eight mangrove species(Sonneratia apetala, Kandelia obovata, Bruguiera gymnorhiza, Aegiceras corniculatum, Acanthus ilicifolius, Acrostichum aureum, Heritiera littoralis and Hibiscus tiliaceus)under different light intensity treatments(100%, 45%, 30%, and 10% of natural light intensity)were studied using shading control experiment. The results were as follows:(1)Low light intensities had little effect on the leaf soluble protein contents of Bruguiera gymnorhiza, Acanthus ilicifolius and Acrostichum aureum, whereas the other five species showed a decreasing trend of leaf soluble protein contents with the decline in light intensity.(2)The activities of superoxide dismutase(SOD)and ascorbate peroxidase(APX)in the leaves of Bruguiera gymnorhiza, Acanthus ilicifolius and Acrostichum aureum under 10% of natural light intensity treatment had no significant differences compared with the control, while the other five mangrove species showed a decreasing trend of all the five antioxidant enzyme activities. In conclusion, the results indicate that Bruguiera gymnorhiza, Acanthus ilicifolius and Acrostichum aureum are suitable to be planted under the forest with high canopy density, while Sonneratia apetala, Kandelia obovata, Aegiceras corniculatum, Heritiera littoralis, Hibiscus tiliaceus are suitable to be planted under the forest with lower canopy density or planted as middle and upper layer tree species in mangrove. The results of this study provide theoretical guidance for the optimal allocation of artificial mangrove communities. [ABSTRACT FROM AUTHOR]

The article researched simulation of the melted metal die forging process of temperature field, velocity field, shrinkage porosity and oxides of femoral stem of CoCrMo alloy and effects of casting temperature, mold temperature and forging speed on the filling and solidification with the help of the software ProCAST. The results indicated molten metal impact ran into the wall of the mold cavity and formed turbulent flow in the neck and head of the femoral stem, which posed risk of gas entrainment. Forging speed has obvious influence on oxides: the higher of forging speed, the lower of oxides contents. The molten alloy ran into the wall of the mold cavity and formed turbulent flow in the neck and head of the femoral stem whose top appeared oxides-rich area. CoCrMo alloy femoral stem whose casting was complete without shrinkage porosity have been gotten by injecting inert gas into the mold cavity before molten alloy pouring and setting the exhaust groove at the front of the femoral stem and exhaust plug at the highest position of the head of femoral stem. [ABSTRACT FROM AUTHOR]

Background and purpose: Anti-programmed death-1 (anti-PD-1) adjuvant therapy and targeted therapy have resulted in longer recurrence-free survival (RFS) for malignant melanoma patients in clinical trials. However, the efficacy of adjuvant anti-PD-1 therapy in Chinese melanoma patients has not been previously reported. The purpose of this study was to retrospectively analyze the RFS in Chinese resected stage III malignant melanoma patients treated with adjuvant anti-PD-1 therapy and to preliminarily compare it with BRAFV600 inhibitor vemurafenib. Methods: This study retrospectively collected the information of 120 patients with resected stage Ⅲ malignant melanoma from three major cancer centers in China from August 30, 2018 to August 30, 2020. These patients received adjuvant pembrolizumab/toripalimab or vemurafenib. RFS rates were assessed by Kaplan-Meier curves and log-rank tests were used to examined differences. Results: Among the 90 patients with stage III malignant melanoma receiving adjuvant anti-PD-1 therapy, the median-RFS (mRFS) was 16 months (95% CI: 14-18 months); the 12-month RFS rate was 69.5% (95% CI: 64.6%-74.4%) and the 24-month RFS rate was 45.8% (95% CI: 40.5%-51.1%). The mRFS for 30 patients who received vemurafenib was 18 months (95% CI: 10-21 months), the 12-month RFS rate was 62.8% (95% CI: 54.0%-71.6%), and the 24-month RFS rate was 32.9% (95% CI: 27.1%-44.7%). In the stratified analysis, firstly, there was no difference in RFS between anti-PD-1 therapy group and vemurafenib group. Secondly, the BRAFV600E mutation status may did not affect the RFS of anti-PD-1 therapy. In addition, in this study, for the patients with BRAFV600E mutation, the RFS showed no difference between these patients received anti-PD-1 therapy or vemurafenib. Finally, although the RFS was better in patients with cutaneous melanoma than in patients with acral and worst in patients of unknown origin, the data for adjuvant therapy in China were still poor. Conclusion: These are the real-world data from adjuvant anti-PD-1 therapy in patients with resected stage III cutaneous and acral melanoma patients in China. In our study, adjuvant anti-PD-1 therapy is indistinguishable from vemurafenib indicates more combination therapies are needed to improve outcome in China. [ABSTRACT FROM AUTHOR]

Waterborne polyurethane (WPU) antibacterial film was prepared by UV curing after introducing hydrophilic quaternary ammonium salts to polyurethane (PU) molecular chains via quaternization reaction between different alkyl chain haloalkanes and tertiary amine groups. PUs were bonded with tertiary amine group and unsaturated group, using 3-dimethylamino-1,2-propanediol as the chain extender and hydroxyethyl methacrylate as the capping agent. The structure and properties of WPU dispersion and light cured films were characterized by FT-IR, particle size analyzer, optical contact angle instrument, etc. The antibacterial properties of the materials were tested by shaking-flask method and zone of inhibition. And the effects of tertiary amine compounds on the properties of WPU were investigated. When the mass fraction of tertiary amine compound was 9% and the alkyl long chain was 12, the antibacterial rates of WPU against Gram-negative E. coli and Gram-positive S. aureus were more than 99.5%. Meanwhile, the longer the alkyl chain was, the better the effect was. Through UV-curable crosslinking, it could still maintain excellent antibacterial performance and reflect positive durability after 96 h water washing. The zone of inhibition test showed that the prepared quaternary ammonium salts WPU antibacterial film belonged to contact-killing material which avoided the pollution and adverse effects on the environment, human beings and other materials. With the increase of tertiary amine content, the quaternization degree of WPU system first increased and then decreased, and the variation of quaternization degree affected the antibacterial properties. Moreover, polyurethane material also exhibited good mechanical properties. The increase of hard segment content of the system enhanced the tensile strength of polyurethane. And when the alkyl long chain was 12, the waterborne polyurethane material had better toughness. [ABSTRACT FROM AUTHOR]

The soil fungal community plays an important role in maintaining soil fertility and plant health，however，the effect of bacterial wilt from Ralstonia solanacearum infection on the soil fungal community still is little. Real-time quantitative PCR and high-throughput sequencing technology of MiSeq were used to study the fungal community composition in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato. The results showed that the occurrence of R. solanacearum infection significantly affected the fungal community composition of bulk and rhizosphere soil. Compared to the non-diseased tomato rhizosphere soil，the fungal abundance，alpha diversity of Chao1 and Shannon in the diseased tomato rhizosphere soil significantly reduced. And the relative abundances of indigenous beneficial fungi such as Gliocladium，Mortierella，Acremonium and Trichoderma significantly reduced whereas the relative abundance of the harmful fungus of Fusarium significantly increased. In summary，the occurrence of R. solanacearum infection altered the composition of the soil fungal community. The fungal abundance，alpha diversity，and the relative abundance of indigenous beneficial fungi depleted while the relative abundance of harmful fungi enriched. The results may provide the elucidation of the microecological mechanism of outbreak of soil-borne bacterial wilt. [ABSTRACT FROM AUTHOR]

Inflammation caused by chronic liver is primarily responsible for the occurrence and pathological progression of liver fibrosis. In the process of liver fibrosis, a large number of activated inflammatory signals promote the transformation of hepatic stellate cells (HSC) into myofibroblasts (MF), which eventually leads to the massive secretion and deposition of extracellular matrix (ECM) and the formation of scar tissue in the liver. To provide literature references for clinical diagnosis and treatment, this paper reviews the roles of HSC, Kupffer cells (KC), inflammasomes and inflammatory signaling in liver fibrosis. [ABSTRACT FROM AUTHOR]

With the "carbon neutrality and emission peak" goal being put forward, the integrated energy system (IES) based on photovoltaics/thermal, etc. has developed rapidly. In order to improve the economic benefits of energy-consuming units and the low carbon performance of the system, optimal operation of energy supply and consumption system in plant area considering multi-energy power generation under carbon emission constraints, needs to be studied urgently. Firstly, in the economic dispatch model, a carbon trading mechanism was introduced, and a ladder-type carbon trading cost model was constructed to constrain the carbon emissions of the plant. Secondly, a planning model aimed at the minimum sum of system operating costs and carbon transaction costs was established. Markov decision process was used to model economic dispatch based on the least cost, and a distributed proximal policy optimization was used to solve the problem. The cost saving ratio index and carbon dioxide emission reduction index was adopted to analyze the economics and carbon emission reduction of the proposed scheme. The results show these as follows. The trained model can handle various operational scenarios represented by the changes of the four random variables without recomputing. The photovoltaic/thermal subsystem and the cogeneration system have a peak-shaving and valley-filling effect. It can improve the stable operation capability of the system. The co-production system designed with carbon trading is beneficial to optimize the energy structure of the plant area and can improve the consumption of clean energy. [ABSTRACT FROM AUTHOR]

Objective　To explore the influence and relationship of prepregnancy weight and pregnancy weight gain on pregnancy complications and adverse pregnancy outcomes， and to provide the evidence for the pregnancy health care， reducing pregnancy complications and improving maternal and infant outcomes. Methods　According to the retrospective study method，603 singleton live pregnancies who were hospitalized and delivered in People’s Hospital of Xishuangbanna Dai Autonomous Prefecture with gestational diabetes mellitus （GDM）, hypertension and other diseases and fetal macrosomia during January 2020 to January 2022 were collected. According to the prepregnancy body mass index （BMI） and gestational weight gain （GWG） criteria，they were divided into the low weight group （ n = 31）, normal weight group （ n = 365）, super weight group （ n = 146）, obesity group （n = 61）, underweight gain group （n = 163）, normal weight gain group （n = 230） and excessive weight gain group （n = 210）：The pregnancy complications and adverse pregnancy outcomes，such as cesarean section rate，shoulder dystocia，birth rate of macrosomia，low birth weight infants，and admission rate to NICU were compared and analyzed. Results　Prepregnancy maternal obesity was the influencing factor of preeclampsia. The incidence of preeclampsia，cesarean section，preterm delivery，low birth weight and NICU transfer increased with the increase of BMI before the pregnancy，and the difference was statistically significant （ P < 0.05） . The incidence of macrosomia， shoulder dystocia， preeclampsia and cesarean section increased with the increase of GWG，and the difference was statistically significant （P < 0.05）. Conclusion　High BMI before the pregnancy and excessive weight gain during the pregnancy can increase the incidence of pregnancy complications and lead to the increase in the incidence of adverse pregnancy outcomes. We should further strengthen the management and education of pregnant women’s weight during the pregnancy，and carry out whole-process intervention on weight during the pregnancy. [ABSTRACT FROM AUTHOR]

For the lack of pigment in oculocutaneous albinism (OCA), especially in the iris and choroid， the diagnosis and surgical intervention of retinal detachment in albinism patients is extremely difficult. Because of the loss of melanin in the fundus of OCA patients，the“red fundus”lacks contrast，and the retinal tissue is difficult to recognize，both fundus examination and surgical operation are very difficult. Due to the long time of postoperative retinal healing，the management of complications also needs to be very careful. This report introduces the pathogenesis，diagnosis and treatment of rhegmatogenous retinal detachment in an albinism patient. With treatment，the patient's retina was successfully reattached and there was no recurrence during a 3-month follow-up. However，due to secondary glaucoma optic nerve damage， the patient ended up with a poor prognosis. The patient was eventually assessed for disability. This case suggests that the therapeutic effect of retinal detachment in albinism patients is still not satisfactory despite the most careful surgical treatment. In the future，clinicians should continue to explore more effective diagnosis and treatment strategies. [ABSTRACT FROM AUTHOR]

Objective: To discuss the clinical features, diagnosis, prognosis and tissue sources of prepubertal-type testicular neuroendocrine tumor. Methods: A case of prepubertal-type testicular neuroendocrine tumor was reported. Simultaneously, 48 cases reported in China were retrieved for literatures review. Results: The left testicle of the patient was swollen for 8 years, with progressive growth and pain. Imaging examination diagnosis maybe testicular germ cell tumor. The pathological diagnosis was a testicular neuroendocrine tumor, prepubertal-type and postoperative imaging excluded metastasis. During 3 years following-up, the patient had no recurrence or metastasis. Conclusion: Pprepubertal-type testicular neuroendocrine tumor is a rare neuroendocrine tumor, and accounts for about 0.23% of testicular tumors. Clinical manifestations and images of it are not specific, the diagnosis mainly depends on pathology, and the treatment is mainly radical surgery, and the prognosis is good. [ABSTRACT FROM AUTHOR]

Proanthocyanidins (OPC) extracted from natural plants was used as free radical trapping agent and blended with polyvinyl alcohol (PVA). The PVA/OPC composite films were produced by solution casting. The thermodynamic stability of the composite films was measured by using differential scanning calorimetry (DSC) and thermogravimetric analyser (TGA). The properties of UV shielding and light transmission were investigated using double-beam UV-Vis spectrophotometer (UV-Vis). And the crystalline properties were characterized using small angle X-ray scattering (SAXS). The results show that the addition of OPC inhibited the crystallization of PVA, the thermal stability and the melt processing window of the composite films were effectively improved by adding 1.0%-2.0% OPC. And the PVA/OPC composites films exhibited excellent mechanical properties, light transmittance and UV shielding properties. [ABSTRACT FROM AUTHOR]

Objective* To compare the ability of VDW Reciproc Blue (Blue), FKG XP-endo shaper (XP) and M3-L platinum (M3) instruments to maintain the original curvature of root canal (i. e. central positioning ability) in preparing curved root canals. Methods • Thirty resin-simulated curved single root canals were randomly divided into three groups. Root canal preparation was performed with a Blue file, an XP file or an M3 file randomly. Red or blue-black ink was injected into the root canal before and after preparation and photographs were taken under the stomatology surgical microscope. The images before and after preparation were overlapped. The apex of the prepared root was set as a circle center and the radius started at 1 mm. A concentric circle was made every 1 mm increased and sequentially incremented to 10 mm so that it intersected the image to form 10 measurement sites, and then the removal of ten measurement sites on the inside and outside of the root canal was recorded for statistical analysis. After the root canal was prepared the file was observed for tensile deformation under the stomatology surgical microscope. Results • There was significant difference in the absolute value of the difference between the internal and external removal of root canal between the M3 group and the Blue group, as well as the M3 group and the XP group (P=0.003 1, P=0.002 0); but there was no significant difference between the Blue group and the XP group (P=0.350). The file body of XP group was observed to have de-helix deformation under the stomatology surgical microscope, which did not return to its original shape when placed in hot water, and no deformation was observed in the file body of M3 group and Blue group. Conclusion • The three kinds of single-file nickel-titanium instruments have good formation ability when preparing the curved root canals. But XP file and Blue file can maintain the original curvature of root canals better than M3 file. XP files are prone to deformation when preparing resin root canal, therefore the use of XP should be appropriately reduced clinically. [ABSTRACT FROM AUTHOR]

Signal transduction and transcriptional activator 3 (STAT3) is an important transcription factor that can be activated by many cytokines and growth factors. STAT3 plays a key role in cell growth, proliferation, and differentiation. It has been shown that hyperactivation of STAT3 exists in almost all animal models of liver injury and human liver diseases. Therefore, inhibition of STAT3 activation might become a promising strategy for prevention and treatment of acute liver injury and liver fibrosis. The research progress of STAT3 on liver injury, hepatitis, liver regeneration, liver fibrosis, and hepatocellular carcinoma were mainly discussed in this review. [ABSTRACT FROM AUTHOR]

In order to reveal the law for forced vibration steady-state response of elastic cylindrical shell theoretically, based on the Reissner shell theory, the forced vibration equations of elastic cylindrical shell under the frame of Hamiltonian system are established and solved by using symplectic analytical method. The natural frequency and vibration mode of cylindrical shell are reduced to the symplectic eigenvalues and symplectic eigensolutions in Hamiltonian system. Further, the solution of the forced vibration steady-state response is transformed into the solution of algebraic equations. Then, the expression of the analytical solution of the forced vibration problem can be obtained. The results of stady show that the amplitude for forced vibration of cylindrical shell is related to the excitation load, the geometric parameters and material. The validity and accuracy of the present approach are verified by a comparison study. The results may provide a theoretical basis for the engineering application of cylindrical shell structure. [ABSTRACT FROM AUTHOR]

在慢性萎缩性胃炎的治疗环节中，讨论消化内科的治疗方法所获得的治疗实际效果。方法：选 择 2020 年 6 月-2021年6月在山东省沂水县龙家圈街道卫生服务中心开展漫性萎缩性胃炎症状治疗的病人总共104例，把 所有病人任意划分成2个工作组，每一个小组病人总数同样，观察组接纳消化内科治疗，而对照实验组选用基本药品 的治疗方式，对于 2 组病人治疗结论及其副作用产生问题进行数据分析。结果：观察组治疗高效率高，副作用发病率 低，数据对比分析 P < 0.05。结论：对漫性萎缩性胃炎病人执行消化内科治疗不但可以提升功效，确保治疗安全性， 并且能够降低副作用发生率，非常值得临床医学普及化。 [ABSTRACT FROM AUTHOR]

The experiment was to explore the effect of Acremonium terrestris culture added in diet on production performance and serum indexes of lactating dairy cows. 40 Holstein lactating cows were selected and randomly divided into control group and test group, with 20 repetitions in each group. The cows in control group were fed with basal diet, and the test group was added 30 g/(head.d) ATC into basal diet. The pre-feeding period was 15 d, and the formal test period was 60 d. The results showed that the average dry matter intake of cows in test group was significantly higher than that of control group (P<0.05), the milk production was extremely higher than that of control group (P<0.01), and the nitrogen utilization efficiency was significantly higher than that of control group (P<0.05). The milk protein production and lactose production of test group were extremely higher than those of control group (P<0.01). The content of TG, TP, ALB and the activity of SOD in test group were significantly higher than those in control group (P<0.05). The experiment indicates that the addition of 30 g/(head.d) of Acremonium terrestris culture in diet can improve the production performance, serum biochemical indicators and antioxidant functions of dairy cows. [ABSTRACT FROM AUTHOR]

Network representation learning aims to transform the nodes in the network into a low-dimensional vector space while maintaining the inherent properties of the network. Most of the existing methods are aimed at normal networks, ignoring the particularity of attribute bipartite networks and the highly non-linear characteristics of the network. To solve the above problem, this paper proposed a deep attributed bipartite network embedding method incorporating with multilevel structure information. Specifically, the algorithm introduced an extended weight matrix to fuse the explicit and implicit structure of the bipartite network with attribute information. Then, it utilized a deep auto-encoder model to capture the highly non-linear characteristics of the network. To maintain the global network structure, a deep auto-encoder reconstructed the second-order proximity. Meanwhile, the deep auto-encoder used the first-order proximity of the nodes as supervisory information to maintain the local network structure. Finally, the algorithm performed joint optimization to get the final representation vectors of nodes. The model was executed on the four datasets of Yelp, Douban Book, Douban Movie and MovieLens. Compared with the latest benchmark method, the average values of F1@10, MAP@10,MRR@10 and NDGG@10 of this model have improved by 4.29%、5.63%、6.26%、4.21%. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the efficacy of Escitalopram oxalate tablets combined with modified electroconvulsive therapy (MECT) in patients with bipolar disorder and depressive episode and its effects on serum brain-derived neurotrophic factor (BDNF). METHODS: This study was a prospective randomized controlled trial, which started in Mar. 2019 and ended in Oct. 2020. A total of 114 patients with bipolar disorder and depressive episode admitted into Jining Psychiatric Hospital were extracted, and the sample size was determined by the sample size of the superiority test. Excel function was used to generate 114 random numbers, and patients were assigned according to the order of admission. Odd and even numbers were set as control group and study group respectively. During the clinical investigation, there were 7 cases of missed and lost follow-up, and 107 cases completed the experiment, including 53 cases in the control group and 54 cases in the study group. The control group was treated with MECT, while the study group received Escitalopram oxalate tablets combined with MECT. The depression status of two groups before treatment and after treatment of 8 weeks was evaluated by Hamilton Depression Scale-17 (HAMD-17), and the serum BDNF level was detected, cognitive function was assessed by Trail Making Test (TMT), the efficacy was evaluated and the incidence of adverse drug reactions was analyzed. RESULTS: The total effective rate of the study group was 92. 59% (50/54), significantly higher than 71. 70% (38/53) of the control group, with statistically significant difference (P<0. 05). After treatment, the HAMD-17 score of two groups was significantly lower than that before treatment, and the HAMD-17 score of the study group as lower than that of the control group, with statistically significant difference (P<0. 05). After treatment, the serum BDNF level of two groups was significantly higher than that before treatment, and the serum BDNF level of the study group was significantly higher than that of the control group, with statistically significant difference (P<0. 05). After treatment, TMT-A and TMT-B of two groups were significantly shorter than those before treatment, and those of the study group were significantly shorter than those of the control group, with statistically significant difference (P < 0. 05). There was no significant difference in the incidence of headache, nausea, vomiting, dizziness, short-term memory disorder between two groups (P > 0. 05). CONCLUSIONS: The efficacy of Escitalopram oxalate tablets combined with MECT in patients with bipolar disorder and depressive episode is significant, which can significantly alleviate the depression, effectively increase the level of BDNF and improve the cognitive function with higher safety. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of early fluid resuscitation on systemic inflammatory response in patients with severe acute pancreatitis (SAP). Methods The 44 SAP patients admitted to the ICU of the hospital from May 2016 to May 2019 were subjected to restrictive volume resuscitation, continuous blood purification, somatostatin and gabexate inhibit pancreatic secretion, inhibit acid, prevent infection, and control Standard treatments such as blood sugar, correction of internal environment disorders, and traditional Chinese medicine enemac Observing the patient's total fluid balance within 72 hours of treatment, lactic acid level, abdominal pressure, oxygenation index, interleukin-6(IL-6)and tumor necrosis factor-α (TNF-α) levels, and symptom relief after 72 hours. Results Fluid balance within 72 hours was negatively correlated with oxygenation index after treatment (P <0. 05), and positively correlated with abdominal pressure, IL-6 and TNF- αlevels (P <0. 05), fluid balance within 72 hours was an early symptom Relevant factors can be alleviated (P <0. 05). Conclusion In the early treatment of SAP patients, the use of fluid resuscitation to reduce the positive fluid balance can reduce the patient's systemic inflammatory response. [ABSTRACT FROM AUTHOR]

Trastuzumab deruxtecan (DS-8201a) is a novel antibody-drug conjugate (ADC) of human epidermal growth factor receptor 2 (HER2) targeted antibody and DNA topoisomerase Ⅰ inhibitor (deruxtecan, DXd). DS-8201a has the characteristics of loading new cytotoxic drugs, high proportion of drug antibodies and high cell membrane permeability. Compared to traditional anti-HER2 drugs only aimed at patients with overexpression of HER2, DS-8201a can also play a role in tumors with high and low expressions of HER2. So far, phase Ⅰ to Ⅲ clinical trials have been carried out in a variety of solid tumors, such as breast cancer, gastric cancer, colorectal cancer and non-small cell lung cancer. Studies have shown results, including indications, toxicity and safety, determination of effective dose, comparison with existing first-line and posterior-line standard chemotherapy regimens, and efficacy comparison between monotherapy and combination therapy. It may provide a new therapeutic method for clinical treatment of tumors with overexpression or low expression of HER2, and pulmonary symptoms should be monitored during use. In this review, the drug structure characteristics, related clinical research progress and prospect of DS-8201a were summarized. [ABSTRACT FROM AUTHOR]

The dynamic study of community structure in karst area is a key problem to be solved in ecological reconstruction and rocky desertification control. Based on the monitoring data of six permanent monitoring sample plots in Puding County of Central Guizhou Province in 2013, 2015 and 2019, the dynamic characteristics of important value, species fluctuation structure, species diversity, diameter class structure and vertical structure of karst secondary forest in seven years were analyzed. The results were as follows:(1)The dominant degree and regeneration rate of Kalopanax septemlobus and Celtis sinensis increased, while the important values of Zanthoxylum armatum, Campylotropis macrocarpa and Pyracantha fortuneana decreased, and the mortality rate was higher.(2)There was no significant differences in species diversity indexes between different years. Species richness and Margalef richness index increased first and then decreased. Shannon-Wiener diversity index was basically stable, and uniformity index decreased first and then increased, the differences were significant between 2013 and 2019.(3)The distribution of diameter class and tree height showed “inverted J” and “left partial normal” distribution, and there was no significant differences in different years. The number of individuals of large diameter class(DBH≥10 cm)increased, but the number of tall trees(H≥9 m)decreased. In the shrub layer, the number of individuals of Pyracantha fortuneana, Campylotropis macrocarpa and Rhamnus rosthornii at 0≤H<3 m and 0≤DBH<2.5 cm, decreased, while the number of individual tree species such as Kalopanax septemlobus and Celtis sinensis increased. The natural succession process of karst secondary forest is slow and complex, and the hierarchical structure is not obvious. The community is in the middle and early stage of succession, and the community regeneration is good, which will further develop in the direction of arbor dominance [ABSTRACT FROM AUTHOR]

Based on the homogeneity of knitted sweater design in the market and the lack of originality of patterns, the pattern design rule of positive and negative stitches has been studied by method of contrastive experiment. Geometric patterns, animal patterns, and embossed patterns that are widely used in pattern design have been creatively designed and used in the cases of the design of different sweaters. The research shows that the patterns formed by different combinations of positive and negative stitches are regular, and the appearance of positive and negative stitches fabric pattern is related to the density of the needle arrangement, the direction and distribution of the pattern. The pattern designed by using this rule can be used in the sweater design of many styles, such as leisure and business, making the sweater more personalized. [ABSTRACT FROM AUTHOR]

In this paper, raspberry-like polydopamine/copper nanoparticles (PDA-Cu NPs) were constructed by simply adjusting the contents of copper source and reduction temperature on the basis of good adhesion properties of polydopamine (PDA) with metal particles. The successful introduction of copper nanoparticles was confirmed by measurements of X-ray diffraction (XRD) and Zeta potential. Scanning electron microscopy (SEM) results showed that the raspberry-like structure had been successfully constructed. When the reduction temperature was 60 ℃, the copper nanoparticles had more uniform particle size. When the mass ratio of PDA to copper nitrate was more than 1/6, the copper nanoparticles were evenly distributed on PDA particles. The static contact angle measurements revealed that the introduction of copper nanoparticles increased the surface roughness of the PDA particles, and the surface contact angle was up to 102.2°, showing that the modified particles turned from hydrophilic to hydrophobic. At the same time, copper nanoparticles had good antibacterial properties. The antibacterial activity of raspberry-like nanoparticles was evaluated by shake flask method. Compared with pure dopamine nanoparticles, PDA-Cu NPs had excellent antibacterial properties. With the increase of copper content, 100% sterilization could be achieved, and its antibacterial performance against S.epidermidis was better than that of E.coli. The bacterial zone experiments showed that PDA-Cu NPs (1/6, mass ratio of PDA to copper nitrate) had a clear zone of inhibition and its diameter was larger than that of PDA-Cu NPs (1/8), suggesting the optimal antibacterial properties. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of raspberry-like nanoparticles made from optimal reaction conditions for E.coli were 48.7 μg/mL and 195.0 μg/mL, respectively, while the MIC and MBC values for S.epidermidis were 39.0 μg/mL and 97.5 μg/mL, respectively. Raspberry-like nanoparticles showed good abtibacterial properties, which were expected to have a good application in biomedical fields. [ABSTRACT FROM AUTHOR]

To investigate the therapeutic effect of Tongbi capsule on rheumatoid arthritis (RA) and its influence on microcirculation index, matrix metalloproteinase-1 (MMP-1) and serum matrix metalloproteinase-3 (MMP-3). 90 patients with RA admitted to our hospital from January 2016 to September 2018 were selected, they were divided into study group (n=45), control group (n=45) according to the random number table method. The control group was treated with leflunomide and methotrexate. The study group was treated with Tongbi capsule on the basis of the control group. The therapeutic effect, microcirculation indexes before and after treatment [Vascular resistance index (RI), diastolic peak velocity (EDV) and systolic peak velocity (PSV)], MMP-1, MMP-3, Rheumatoid factor titer (RF), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), antistreptolysin "O" (ASO) of the two groups were compared, adverse reactions were recorded. The total clinical effective rate of the study group was higher than that of the control group (P<0.05). The RI, MMP-1, MMP-3, CRP, RF, ESR and ASO of the two groups decreased after 4 months of treatment, and the study group was lower than the control group (P<0.05); EDV and PSV increased, and the study group was higher than the control group (P<0.05). There was no difference in the incidence of adverse reactions between the two groups (P>0.05). on the basis of routine treatment, Tongbi capsule can effectively alleviate RA, improve microcirculation, reduce the levels of MMP-3 and MMP-1 in serum, and the drug safety is good. [ABSTRACT FROM AUTHOR]

With emphasis of Chinese government on AI and robot industry, general demand on intelligent production and life is characterized in the time of industry 4. 0. Thereafter robotics is developed at an unprecedented speed, which leads to serious lack of specialist. In recent years, robots have been gradually introduced into primary schools, secondary schools and even university education as a novel curriculum. However, the establishment of undergraduate robot specialty in domestic universities is relatively late so that it is difficult to evaluate the effect of robot education. The popularity of science education curriculum in primary and secondary schools is still lacking case of robot. This paper puts forward a novel architecture of robot subject for the integration of production, teaching and research while analyzed the specific direction and curriculum and content settings of talent training based on the position and talent demand of the employers and the practical large-scale sustainable projects of the industrialization of education robot. Various levels of robot course were drawn up including scientific popularization education series, higher education series and advanced study series by integrating classroom teaching, scientific research, industrialization project and work position demand . Consequently, the robot discipline construction can serve the society effectively, create scientific research achievements as well as train the specialized and application-oriented talents urgently needed by the enterprises. [ABSTRACT FROM AUTHOR]

Objective To analyze the image features of shear wave elastrography (SWE) in breast masses, and to evaluate their values in the differentiation of benign and malignant breast lesions. Methods A total of 361 patients with 403 breast lesions who simultaneously underwent conventional ultrasound and SWE examination from February 2015 to January 2018 were selected. Diagnosis in all patients was confirmed by aspiration biopsy or operative pathology. The SWE images were collected and the elastic images were divided into 5 types. The SWE image features of different breast pathological types were summarized, and their values in benign and malignant breast lesion diagnoses were evaluated. Results The main features of benign breast lesion were type I and II, the main features of the malignant lesion were type IV and V, and the proportion of which were 43.6% (71/163), 37.4% (61/163), 22.1% (53/240) and 57.9% (139/240), respectively. Type III accounted for a certain proportion in both benign and malignant lesions. The SWE image features of benign and malignant lesions were compared and a significant difference was observed (P<0.001). The type V features were mainly observed in invasive ductal carcinoma, invasive lobular carcinoma and other types of invasive carcinoma, while the type IV features were mostly presented in ductal carcinoma in situ and mucinous carcinoma. Fibroadenoma, fibroadenosis accompanied with fibroadenoma, and fibroadenosis were featured with type I. Both intraductal papilloma and benign phyllodes tumor were mostly type II, while type III and V were more common in chronic granulomatous mastitis. When type I and typeIIof breast lesions were classified as benign features while type IV and V were malignant features, the sensitivity and specificity of breast malignant lesion diagnosis were 91.2% and 84.7% by application of SWE combined with breast imaging reporting and data system (BI-RADS). The sensitivity of combined diagnosis was slightly lower than that of conventional ultrasound (P>0.05), but the specificity was significantly higher than conventional ultrasound (P<0.01). Conclusion The SWE is a simple and effective method. Combination of SWE with conventional ultrasound may improve the diagnostic differentiation of benign and malignant breast lesions. [ABSTRACT FROM AUTHOR]

For achieving the requirement of fish production and water sustained regeneration in controlled ecological life support system, a pilot-scale modified biological aerated filter (BAF) coupled with vegetable cultivation system was constructed as a purification plant for the closed recirculating aquaculture system (RAS). The performance of the modified purification process on RAS was investigated, and its principle was also discussed in this research. At initial stage, 300 L municipal water as well as 60 tilapia mossambica were inset in the freshwater pond of RAS. In the followed experimental 200 days, the variation of water quality and fish growth was tracked and investigated, and then the navigability of purification process for RAS was analyzed and discussed. The experimental results showed that in the first research period (1-60 days), the single BAF process showed poor performance on the pollutant removal, and there were a mount of TOC, N and P accumulation after 60 days raising period. For resolving this problem, a modified BAF process coupled with vegetable cultivation system was constructed and carried out in the following 140 days. The followed experimental results exhibited that the modified BAF process coupled with vegetable cultivation showed significant good performance on the pollutant control, where the concentration of TOC and NH4+-N decreased form 60.3 and 2.9 mg/L to 11.2 and 1.2 mg/L, respectively, and the concentration of NO3--N and PO43--P obviously decreased form 114.2 and 43.1 mg/L to around 54.7 and 15.6 mg/L, respectively. Besides, the concentration of NO2--N remained below 0.1 mg/L. Modified purification process could provide health-safety environment for the tilapia mossambica growth and vegetable cultivation in closed RAS, more than 95% water was regenerated, and the maximum aquiculture density was 41.3 kg/m3 and without fish death. At the same time, four batches of vegetables were harvested. The total weight of vegetables was 23 420 g, and the individual weight was 195.17 g, respectively. The results showed that the modified BAF process coupled with vegetable cultivation could ensure the sustainable and stable operation of RAS and provide technical reference for fish culture in the controlled ecological life security system. [ABSTRACT FROM AUTHOR]

新型冠状病毒肺炎(以下简称"新冠肺炎”)瘦情期间,采取安全管理措施,全方位保护医护和患者安全,可有效避 免新冠肺炎感染的发生。依赖于完善的制度、唷力的监督、有效的防护及各方人员的积极配合,该院妇产科全体医护人员、妇产科 惠者及家属、所有新生儿均未出现感染。 [ABSTRACT FROM AUTHOR]

For achieving the requirement of fish production and water sustained regeneration in controlled ecological life support system, a pilot-scale modified biological aerated filter (BAF) coupled with vegetable cultivation system was constructed as a purification plant for the closed recirculating aquaculture system (RAS). The performance of the modified purification process on RAS was investigated, and its principle was also discussed in this research. At initial stage, 300 L municipal water as well as 60 tilapia mossambica were inset in the freshwater pond of RAS. In the followed experimental 200 days, the variation of water quality and fish growth was tracked and investigated, and then the navigability of purification process for RAS was analyzed and discussed. The experimental results showed that in the first research period (1-60 days), the single BAF process showed poor performance on the pollutant removal, and there were a mount of TOC, N and P accumulation after 60 days raising period. For resolving this problem, a modified BAF process coupled with vegetable cultivation system was constructed and carried out in the following 140 days. The followed experimental results exhibited that the modified BAF process coupled with vegetable cultivation showed significant good performance on the pollutant control, where the concentration of TOC and NH4+-N decreased form 60.3 and 2.9 mg/L to 11.2 and 1.2 mg/L, respectively, and the concentration of NO3--N and PO43--P obviously decreased form 114.2 and 43.1 mg/L to around 54.7 and 15.6 mg/L, respectively. Besides, the concentration of NO2--N remained below 0.1 mg/L. Modified purification process could provide health-safety environment for the tilapia mossambica growth and vegetable cultivation in closed RAS, more than 95% water was regenerated, and the maximum aquiculture density was 41.3 kg/m3 and without fish death. At the same time, four batches of vegetables were harvested. The total weight of vegetables was 23 420 g, and the individual weight was 195.17 g, respectively. The results showed that the modified BAF process coupled with vegetable cultivation could ensure the sustainable and stable operation of RAS and provide technical reference for fish culture in the controlled ecological life security system. [ABSTRACT FROM AUTHOR]

[Objective] This paper aimed to study the effects of calcium on osmotic regulators and SOD in potato tubers during storage. [Method] A randomized block design was applied to spray Ca(NO3)2 to set 6 levels of calcium fertilizer application. The content of several permeability regulators and the activity of superoxide dismutase (SOD) in potato tubers were determined during storage. [Result] After spraying Ca(NO3)2 in the field, the contents of soluble sugar, proline and other substances in potato tubers were significantly increased, the activity of SOD protective enzymes was significantly increased, and the accumulation of MDA was significantly inhibited. [Conclusion] The comprehensive analysis of the results in this study indicated that the optimum concentration of Ca(NO3)2 was 0.6 kg/hm2. Calcium could effectively improve cell osmotic regulation and protoplast protection, thus improving the storage resistance of potato tubers. [ABSTRACT FROM AUTHOR]

The instant delivery problem in a city area is a new problem arising from the modern electronic commerce. This problem has many new features, e.g. strong dynamics, the urgency in solution time, the one-to-many relationship between retailers and customers, multiple returns of vehicles to retailers for pickups, and the timeliness requirements for pickups and deliveries. The problem cannot be solved by any existing methods. To solve the problem, an online scheduling method based on multiple prediction scenarios is presented. The method brings multiple scenarios, each of which has predicted orders, into the routing and scheduling procedure, and obtains an integrated solution by calculating the scheme of every scenario and integrating the schemes of all scenarios. Such a solution idea improves the elasticity of the scheduling solution to uncertain demands in the future. The proposed scheduling system can make a response immediately when any new order is placed, and optimize the sequence of uncompleted deliveries iteratively by using the large-scale neighborhood search when the system is idle. Finally, the validity and feasibility of the presented method are tested on the real data, which has about 200~300 orders per day and is obtained from an instant delivery company in Dalian City. [ABSTRACT FROM AUTHOR]

With the development of laser technology, more and more materials can be used to make superhydrophobic surfaces. In order to meet the different needs of self-cleaning, anti-fouling and oil-water separation, different materials need to be selected as the substrates for making superhydrophobic surfaces by laser. The metal substrates are of high hardness, stability and durability, and the superhydrophobic surfaces produced from these substrates are excellent in self-cleaning, anti-freezing and anti-fouling. It is also used to create templates for other superhydrophobic surfaces. Inorganic non-metallic materials have a wide variety of properties, thus the performances of superhydrophobic surface are different. Some of these kind materials have good biocompatibility, some are suitable for oil-water separation and manufacturing of super capacitors. The polymer base has good elasticity, low density and friction resistance, and the superhydrophobic materials made from these substrates can be used in industrial manufacturing and microfluidic devices. This review briefly introduces the basic principle of laser manufacturing superhydrophobic and mainly discussed the common bases of laser manufacturing superhydrophobic surface: the metal base, inorganic nonmetallic substrate and the polymer base. The metal base includes aluminum alloy, stainless and copper. Inorganic nonmetals include quartzcrystals and graphene. The polymer substrate includes PDMS polymer and PTFE. The properties and applications of different superhydrophobic surfaces produced by different bases are described in detail. In the end, the application of superhydrophobic surfaces manufactured by laser is summarized and prospected. [ABSTRACT FROM AUTHOR]

In order to identify the sources of the sediment of Xiliugou River and research its transporting along the river, the sediments at the river bed of the mainstream were sampled and the gradation of the grain size of the samples were tested and analyzed. The results show that the sediment gradation of riverbed along the river from upstream to downstream can be divided into three sections, hilly area, desert area and plain area, the median grain size of each section from upstream to downstream changes from coarse to fine. In hilly area the grain size decreases rapidly, then continues to decrease in the desert area and finally remain stable in the alluvial plain with an average median diameter of 2 mm in upstream to 0. 157 mm in downstream. Of the grain size gradation in hilly area, the parts with grain size greater than 0. 5 mm account for more than 70%, but disappear in the plain area. The riverbed in desert area is the transition zone, and the range of grain size is larger. In the plain area, the parts of sediment with grain size of 0. 1-0. 25 mm account for about 62%, and the proportion of sediment with grain size minor than 0. 1 mm increases significantly. Thus, the sediment carried by floods is sorted along the river, and the gravels are mainly deposited in the upper hilly area, and a small part of them further deposited in the desert area. The sediments deposited in the riverbed in the lower plain are mainly from the desert area. [ABSTRACT FROM AUTHOR]