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丙酮醛降低人脑微血管内皮细胞系 hCMEC/D3 细胞活力及线粒体膜电位.
- Source :
-
Basic & Clinical Medicine . Nov2023, Vol. 43 Issue 11, p1631-1635. 5p. - Publication Year :
- 2023
-
Abstract
- Objective To explore the effects and mechanism of methylglyoxal (MGO) in human brain microvascular endothelial cell line (hCMEC/D3). Methods The hCMEC/D3 cells were incubated with MGO (0.25, 0.50, 0.75, 1.00 and 1.25 mmol/L) for 24 h. Cell viability was detected by CCK-8 assay. Cellular LDH and SOD activities were determined by commercially available kits. The change of mitochondrion membrane potential was assessed by JC-1 probe. MitoSOX probe was used to observe mitochondrial reactive oxygen species (mROS) release. Results Compared with control, cell viability of hCMEC/D3 treated with MGO decreased in a concentration-dependent manner (P<0.01). Increased LDH activity and decreased SOD activity were observed with MGO exposure (P<0.01). The decrease of mitochondrion membrane potential and the excessive increase of mROS were induced after MGO exposure for 12 h, which were detected by JC-1 or MitoSOX probe. Conclusions MGO reduces cell viability and mitochondria membrane potential in hCMEC/D3 cells with excessive mROS generation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 43
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Basic & Clinical Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 174154230
- Full Text :
- https://doi.org/10.16352/j.issn.1001-6325.2023.11.1631