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为了解决风味（原香和烤香）油茶籽油掺伪浸出油茶籽油的定性判别问题，设计高、低两个掺伪梯度，基于挥发性成分构建并对比分析了定性判别风味油茶籽油掺伪浸出油茶籽油的主成分分析（PCA）模型和逻辑回归模型。结果表明：逻辑回归模型定性判别风味油茶籽油掺伪浸出油茶籽油的能力较强，优于PCA模型；高掺伪梯度下定性判别原香和烤香油茶籽油掺伪浸出油茶籽油，PCA模型的最低检出限分别为20%和60%，而逻辑回归模型的最低检出限均为10%；低掺伪梯度下定性判别原香和烤香油茶籽油掺伪浸出油茶籽油，PCA模型的判别不准确，而逻辑回归模型的最低检出限均为4%。逻辑回归模型能很好地定性判别风味油茶籽油掺伪浸出油茶籽油。 Based on volatile components, principal component analysis (PCA) and logistic regression (LR)models were constructed and compared to solve the problem of qualitative identification of flavor (original/roasted) oil-tea camellia seed oil adulterated with leaching oil-tea camellia seed oil under high and low adulteration gradients.The results showed that LR model had a good ability to qualitatively identify the flavor oil-tea camellia seed oil adulterated with leaching oil-tea camellia seed oil, which was better than that of PCA model. Under the high adulteration gradient, the detection limit of the PCA model for original/roasted oil-tea camellia seed oil adulterated with leaching oil-tea camellia seed oil was 20%/60%, and the detection limit of the LS model was 10%/10%, respectively. Under the low adulteration gradient, the detection limit of LR model for original/roasted oil-tea camellia seed oil adulterated with leaching oil-tea camellia seed oil was 4%/4%, but the discriminative ability of the PCA model was not accurate.The LR model can qualitatively identify flavor oil-tea camellia seed oil adulterated with leaching oil-tea camellia seed oil.

为解决油茶籽油掺伪其他植物油的定性鉴别问题，在油茶籽油中分别掺入大豆油、花生油、葵花籽油、棉籽油、葡萄籽油、菜籽油、棕榈油和米糠油，设置高和低两种不同掺伪梯度，基于14个特征性脂肪酸和甘油三酯指标，运用Python语言构建并对比分析了二分类决策树模型、多分类决策树模型和多层感知机人工神经网络(MLP-ANN)模型用于油茶籽油掺伪定性鉴别的效果。结果表明：高和低掺伪梯度下，二分类决策树模型对油茶籽油掺伪其他植物油的定性鉴别的准确率均达到0.95以上；多分类决策树模型的精确率和准确率在高掺伪梯度下均达到了0.95，但在低掺伪梯度下仅为0.90；在高和低掺伪梯度下，MLP-ANN模型对油茶籽油掺伪定性鉴别的平均精确率均达到0.98，准确率分别达到0.97和0.98。相比于决策树模型，MLP-ANN模型能很好地实现油茶籽油掺伪定性鉴别。 In order to solve the qualitative identification problem of adulterated oil-tea camellia seed oil with other vegetable oils, soybean oil, peanut oil, sunflower seed oil, cottonseed oil, grape seed oil, rapeseed oil, palm oil and rice bran oil were mixed into oil-tea camellia seed oil respectively, two different adulteration gradients of high and low were set up, and based on characteristic fatty acid and triglyceride indicators, the effects of the binary decision tree model, multi-classification decision tree model and multilayer perceptron artificial neural network (MLP-ANN) model for qualitative identification of adulterated oil-tea camellia seed oil were compared and analysed using Python language. The results showed that the accuracy of the binary decision tree model for qualitative identification of oil-tea camellia seed oil adulterated with other vegetable oils under high and low adulteration gradients was above 0.95. The accuracy and precision of the multi-classification decision tree model reached 0.95 at high adulteration gradient, but only 0.90 at low adulteration gradient. Under high and low adulteration gradients, the average precision of MLP-ANN model for qualitative identification of adulterated oil-tea camellia seed oil reached 0.98, and the accuracy reached 0.97 and 0.98 respectively. Compared with the decision tree model, the MLP-ANN model can well realize the qualitative identification of adulterated oil-tea camellia seed oil.

为了确定酶解法制备家蝇蛹多肽（PHP）的工艺条件，并初步评价PHP的促伤口愈合活性，以家蝇（Musca domestica L.）蛹为原料，采用蛋白酶酶解法制备PHP，通过单因素实验优化酶解法制备PHP的工艺条件，并在细胞水平上评价PHP的促伤口愈合活性。结果表明，10种蛋白酶酶解法制备的PHP中，胃蛋白酶酶解法制备的PHP得率最高，其次是风味蛋白酶酶解法制备的PHP（PHF）。另外，PHF的多肽含量及水解度均最高。单因素实验结果表明，制备PHF的最佳条件为酶解温度60 ℃、加酶量8 000 U/g、酶解时间4 h，在此条件下制得的PHF得率最高、水解最完全。细胞实验结果显示，不同蛋白酶酶解法制备的PHP具有不同程度促进HaCaT细胞增殖及迁移的作用。其中，PHF（分子质量主要分布在500~1 300 Da之间）促伤口愈合活性最佳。同时，PHF可显著促进RAW264.7细胞转化生长因子TGF-β1及炎性细胞因子TNF-α、IL-6的表达。综上，风味蛋白酶酶解法是一种制备高得率、高多肽含量及高水解度的PHP的方法，PHF具有促进伤口愈合的潜力。In order to determine the process conditions for preparing peptides from housefly pupa (PHP) using enzymolysis method and evaluate the wound healing-promoting activity of PHP, PHP were prepared with enzymolysis methods using pupa of Musca domestica L. as the raw material. The single factor experiment was designed to optimize the preparation process of PHP and the wound healing-promoting activity of PHP was studied at the cellular level. The results showed that among the PHP prepared with ten proteases, the yield of PHP prepared with pepsin was the highest, followed by flavourzyme hydrolysis (the peptide was referred to as PHF). In addition, PHF showed the highest peptides content and degree of hydrolysis. The results of single factor experiment displayed that PHF presented the highest yield and degree of hydrolysis under the conditions of temperature 60 ℃, enzyme dosage 8 000 U/g, and time 4 h. Besides, PHP prepared with different proteases had different degrees of promoting proliferation and migration of HaCaT cells, among them, PHF (molecular weight mainly distributed between 500 Da and 1 300 Da) exhibited the best wound healing-promoting activity. Meantime, PHF significantly promoted the expression of transforming growth factor TGF-β1 and inflammatory cytokines TNF-α, IL-6 in RAW264.7 cells. In conclusion, the enzymolysis method with flavourzyme contributes to prepare PHP with high-yield, high-peptides content, and high-degree of hydrolysis, and PHF has the potential to promote wound healing.

近年来，机器学习等人工智能技术被应用于蛋白质工程，其在蛋白质结构、功能预测、催化活性等研究中具有独特优势。在未知蛋白质结构的情况下，将蛋白质序列和功能特性与机器学习相结合，基于序列-活性关系（innovative sequence-activity relationship，ISAR）算法，将蛋白质氨基酸序列数字化，用快速傅里叶变换（fast four transform，FFT）进行预处理，再进行偏最小二乘回归建模，可在数据集较少情况下拟合得到最佳模型。通过机器学习对紫色球杆菌视紫红质（gloeobacter violaceus rhodopsin，GR）的突变体蛋白质氨基酸序列与光谱最大吸收波长进行建模，获得了最佳模型。用最佳索引LEVM760106建模得到的确定系数R2为0.944，均方误差E为11.64。用小波变换进行的预处理，其R2虽也约为0.944，但E大于11.64，不及FFT进行的预处理。方法较好地解决了蛋白质序列与功能特性之间的数学建模问题，在蛋白质工程中可为预测更优的突变体提供支持。

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慢性胰腺炎 (chronic pancreatitis, CP) 是多种因素导致的慢性消化系统疾病, 胰腺纤维化是其典型的 病理改变, 最终可导致胰腺内外分泌功能紊乱。CP 患者胰腺外分泌功能紊乱可造成肠道免疫屏障破坏, 导 致肠道微生态失衡, 而肠道微生态失衡又在 CP 发展过程中发挥重要作用。肠道微生态失衡可引起 CP 患者 小肠细菌过度生长, 使肠道菌群多样性减少、病原菌增多, 有益菌及其代谢产物短链脂肪酸、乳酸和丁酸等 减少, 且过度聚集的内毒素又可引发 CP 患者糖代谢异常, 增加 CP 患者癌变的风险。通过胰酶替代疗法、抗 菌药物治疗、益生菌治疗及中医药治疗等方法调节肠道微生态平衡, 缓解 CP 进展, 有望成为治疗 CP 的有效 手段。 [ABSTRACT FROM AUTHOR]

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This paper summarized the practice and experience of shift rotation in the general ward, CCU and catheterization unit of cardiology. The practice of shift rotation included rotation background of rotation, setting up of a team of clinical teachers, establishing the teaching program, training before shift rotation and related assessment. Nurses were trained in professional skills, and implementation of shift rotation had relieved pressure on nursing human resources. (本文主要对心内科普通病房、CCU和导管室3个护理单元实施轮转的相关经验进行总结, 主要包括轮转的背景、带教老师的确定、教学内容的制定、轮转前的培训以及相关考核等, 通过实施轮转, 护士的业务能力明显提高, 缓解了科室人力资源紧张的情况。)

Hypertension is a common chronic disease, and headache, as one of the clinical symptoms of hypertension, has recurrent attacks and seriously affects the quality of life of patients. In recent years, studies have applied the combination of auricular pressure massage, ear tip bloodletting and auricular point pressing to treat hypertensive headache with good efficacy, improving patients' comfort and quality of life, which is widely used in clinical practice. This article reviewed studies on the efficacy of combined auricular acupoint therapy in the treatment of hypertensive headache patients. (高血压是一种常见慢性疾病, 头痛作为高血压的常见临床症状之一, 反复发作会严重影响患者生存质量。近年来, 耳穴按摩、耳尖放血、耳穴贴压联合治疗高血压引起的头痛取得了良好效果, 提高了患者的舒适度和生活质量, 在临床上应用广泛。本文综述了耳穴联合疗法治疗高血压患者头痛相关研究, 旨在为临床干预高血压引起的头痛提供参考。)

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This paper summarized the nursing experience of a case of phlebitis caused by peripheral intravenous infusion of amiodarone hydrochloride treated with Traditional Chinese Medicine collapse stains. The patient was treated with Traditional Chinese Medicine collapse stains, and interventions on exercise and mental health were carried out to improve the rehabilitation. Traditional Chinese Medicine collapse stains, characterized by easy-to-operate, safe, wide scope of application, comfort and high rate of acceptance among patients, is effective in treating phlebitis caused by amiodarone hydrochloride injection. (本文总结了1例给予中药溻渍治疗盐酸胺碘酮注射液引发静脉炎患者的护理体会。中药溻渍治疗盐酸胺碘酮注射液引发的静脉炎效果显著, 治疗期间做好活动指导和心理护理, 有助于促进患者康复。中药溻渍具有操作简单、安全有效、适应症广泛、治疗过程舒适、患者易于接受的特点, 值得临床借鉴。)

为了研究中国东北地区貉（Nyctereutes procyonoides）大肠杆菌的流行情况，对56株源自东北地区不同貉养殖场的貉源大肠杆菌进行主要毒力基因的检测与分析，测定其阳性率与分布情况。利用PCR技术法检测貉源大肠杆菌的iucD，irp2，fyuA，fimH，iss，stx1，ybtA，irp1，stx2，eae 10个毒力基因。结果显示iucD，irp2，fyuA，fimH，ybtA的阳性率分别为3929%（22/56），3750%（21/56），5357%（30/56），9821%（55/56），536%（3/56），而iss，stx1，irp1，stx2，eae均未检出。黏附相关基因fimH及HPI毒力岛核心基因fyuA检出率最高，说明此次试验中fimH及fyuA为貉源大肠杆菌的主要毒力基因。

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针对现有室内定位方法，根据目标节点在运动过程中与参考信标节点间产生的多普勒效应，得到一种距离差测量方法，避免了对目标节点与信标节点间时钟同步的要求.为实现此距离差定位，提出了 一种基于拟牛顿法的室内定位算法.随机选取初始猜测值，得到一个测量点的距离差信息，由此迭代得到单个测量点坐标，再将所有测得的相对位置坐标进行整体迭代并调整初始位置，直到得到稳定的初始位置，实现定位.Matlab仿真结果表明，在信噪比SNR=10时，定位误差不超过0.5 m.同时，为提高定位速度和成功率，尝试用粒子群算法求初始猜测值，进一步提高算法的性能.

目的: 探讨经导管主动脉瓣置换术治疗老年重度主动脉瓣狭窄的价值。方法: 选取我院老年重度主动 脉瓣狭窄86名 (时间2022年8月-2023年8月), 以随机数字表法将其划分为观察组和对照组, 各43名。对照组予以 外科主动脉瓣置换术, 观察组予以经导管主动脉瓣置换术。对比两组纽约心脏病协会 (New York Heart Associatio, NYHA) 分级改善情况、主动脉有关指标、心功能指标等结果。结果: 观察组NYHA分级改善总有效率90.70%与对照 组88.37%对比并无显著差异 (P > 0.05) ; 术后3个月, 观察组主动脉瓣流速、主动脉瓣瓣口面积改善情况优于对照组 (P < 0.05) ; 术后3个月, 观察组左心室射血分数 (LVEF) 水平较对照组高, 左心室舒张末期内径 (LVEDD) 水平 较对照组低 (P < 0.05) ; 观察组并发症发生率11.63%和对照组13.95%对比无显著差异 (P > 0.05) 。结论: 经导管主 动脉瓣置换术治疗老年重度主动脉瓣狭窄有较好的临床疗效, 可改善心功能、主动脉瓣情况, 并发症发生率低, 存在 一定的临床应用价值。 [ABSTRACT FROM AUTHOR]

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为提高粒子群算法的收敛速度和优化性能，避免陷入局部最优，提出了一种基于动态学习因子和共享适应度函数的改进粒子群算法.在惯性权重w随着迭代次数非线性减少而动态调整学习因子的基础上，引入共享适应度函数.当算法未达到终止条件而收敛时，利用粒子和最优解间距离挑选一批粒子重新初始化形成新群体，并用共享适应度函数对新群体进行评价，新旧2个群体分别追随自己的局部最优解直至迭代结束.对4个典型多峰复杂函数的测试结果表明，该改进算法不仅加快了寻得最优解的速度，而且提高了粒子群算法全局收敛的性能.

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Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To investigate the diagnostic value of globulin (GLB) level, platelet count ( PLT),and their combination in premature infant with early-onset sepsis (EOS). Methods A total of 365 premature infants admitted to Shiyan Renmin Hospital from January 2017 to December 2021 were selected. A total of 113 infants diagnosed with EOS were selected as the study group, and 252 non-septicemic children with no signs of infection were selected as the control group. Clinical data of the two groups were retrospectively analyzed to explore the diagnostic value of GLB level and PLT in premature infants with EOS. Results The mean birth weight, gestational age, GLB level, album in (ALB) level, and PLT of the infants in the study group were significantly lower than those in the control group, with statistical significance (P < 0.05), and very low birth weight (< 1500 g),low gestational age (≤34 weeks), low GLB level, low ALB level, and low PLT are risk factors for EOS in premature infants. The receiver operating characteristic curve analysis showed that when the GLB level was lower than 15.55g/L, and the PLT was lower than 264.5×109 L-1, it was suggestive for the diagnosis of EOS; the sensitivity of GLB level to the diagnosis of EOS (48.4%) and The specificity (76.1%) was higher than the sensitivity (45.6%) and specificity (73.5%) of PLT. The combined detection of PLT and GLB levels (AUC=0.683) was more valuable in the diagnosis of EOS than the two alone. Conclusion The combined detection of GLB level and PLT is helpful for the early diagnosis of EOS in premature infants, and has a high reference value for clinical diagnosis. [ABSTRACT FROM AUTHOR]

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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