Aim: To gain insight into the current status of peer review guidelines in international surgical journals and to offer guidance for the development of peer review guidelines for surgical journals. Methods: We selected the top 100 journals in the category of 'Surgery' according to the Journal Citation Report 2021. We conducted a search of the websites of these journals, and Web of Science, PubMed, other databases, in order to gather the peer review guidelines published by these top 100 journals up until June 30, 2022. Additionally, we analysed the contents of these peer review guidelines. Results: Only 52% (52/100) of journals provided guidelines for reviewers. Sixteen peer review guidelines which were published by these 52 surgical journals were included in this study. The contents of these peer review guidelines were classified into 33 items. The most common item was research methodology, which was mentioned by 13 journals (25%, 13/52). Other important items include statistical methodology, mentioned by 11 journals (21.2%), the rationality of figures, tables, and data, mentioned by 11 journals (21.2%), innovation of research, mentioned by nine journals (17.3%), and language expression, readability of papers, ethical review, references, and so forth, mentioned by eight journals (15.4%). Two journals described items for quality assessment of peer review. Forty‐three journals offered a checklist to guide reviewers on how to write a review report. Some surgical journals developed peer review guidelines for reviewers with different academic levels, such as professional reviewers and patient/public reviewers. Additionally, some surgical journals provided specific items for different types of papers, such as original articles, reviews, surgical videos, surgical database research, surgery‐related outcome measurements, and case reports in their peer review guidelines. Conclusions: Key contents of peer review guidelines for the reviewers of surgical journals not only include items relating to reviewing research methodology, statistical methods, figures, tables and data, research innovation, ethical review, but also cover items concerning reviewing surgical videos, surgical database research, surgery‐related outcome measurements, instructions on how to write a review report, and guidelines on how to assess quality of peer review. [ABSTRACT FROM AUTHOR]