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Hypoparathyroidism is a frequent and serious complication of thyroid surgery. Identification and preservation of the parathyroid glands are key factors in managing hypoparathyroidism. The aim of the present study was to investigate the efficacy of rapid intraoperative parathyroid hormone (rIO-PTH) assay levels through fine needle aspiration (FNA) in identifying parathyroids as a parameter in thyroid surgery. rIO-PTH assay through FNA and frozen section examination were performed on 194 suspected parathyroids anatomical structures from 50 consecutive patients undergoing thyroidectomy (rIO-PTH group). The association between the rIO-PTH values and histological results were analyzed. Clinical effects were compared between the rIO-PTH and control groups from 50 patients undergoing a similar standard surgery. rIO-PTH levels from 93/194 aspirated anatomical structures certified as parathyroid tissues by histological analysis were demonstrated to have a mean of 3,369 pg/ml (range, 145.2-5,000 pg/ml). These values were significantly increased compared with the mean value of 25.7 pg/ml from non-parathyroids tissues significantly (P<0.001). The mean number of 3.76 on the recognized parathyroids was obtained by naked eye measurements combined with rIO-PTH assay through FNA, was significantly higher than compared with only naked eye measurements (P<0.05). Postoperative permanent or transient hypoparathyroidism was not detected in the rIO-PTH groups. The difference between the postoperative serum calcium level and blood PTH values of rIO-PTH and control groups was not statistically significant (P>0.05). The value of rIO-PTH assay through FNA demonstrated that it is a good parameter for differentiating parathyroids and non-parathyroids tissues. The technique is a highly reliable, quick, simple and non-invasive method with a short learning curve in thyroid surgery, which is particularly valuable for inexperienced surgeons. This method may replace frozen section examination, which relies on a surgeon's personal experience on the basis of topographic or morphologic criteria for recognizing parathyroids. [ABSTRACT FROM AUTHOR]

Background MiR-133b is a muscle-specific microRNA; it has a role in the formation of cardiocytes and the expression of myocardium ion channels by regulating target genes. Many human malignant tumors demonstrate a low expression of miR-133b, as noted in colorectal,lung, esophagus and bladder cancers, but the role of miR-133b in bladder cancer is unknown. Methods The expression of miR-133b in clinical bladder cancer specimens and adjacent normal tissues was confirmed by stem-loop RT-PCR. We also analyzed the relationship between miR-133b expression and clinicopathological factors of bladder cancer. Bcl-w and Akt1 protein expression in 41 bladder cancer specimens and adjacent normal tissues was detected by Western blot. After transfection of miR-133b mimics or inhibitor into a T24 human bladder cancer cell line, Bcl-w and Akt1 protein and mRNA expression were examined by Western blot and RT-PCR, respectively. The effect of miR-133b on T24 cell proliferation and apoptosis was measured by CCK-8 tests and flow cytometry, respectively. Results The expression of miR-133b in bladder cancer tissues from 41 patients was significantly down-regulated (P < 0.01); low expression of miR-133b was strongly associated with highgrade bladder cancer (P < 0.01). Bcl-w and Akt1 proteins were significantly overexpressed in bladder cancer tissues versus adjacent normal tissues (P < 0.01 for both). The expression of Akt1 and Bcl-w proteins and Akt1 mRNA, in T24 cells was significantly down-regulated or up-regulated after transfection of miR-133b mimics or inhibitor, respectively; however, there was no significant difference in Bcl-w mRNA expression. Transfection of HEK-293 T cells with miR-133b significantly suppressed a luciferase-reporter containing the Bcl-w or Akt 1 3'-untranslated regions. MiR-133b mimics significantly inhibited T24 cell proliferation, as well as increased T24 cell apoptosis (P < 0.05 and P < 0.01, respectively) while the miR- 133b inhibitor increased and decreased these, respectively (P < 0.05 for both). Conclusions MiR-133b may play a very important role in the proliferation and apoptosis of T24 cells by regulating the expression of Bcl-w and Akt1. [ABSTRACT FROM AUTHOR]

Since epithelioid blue nevus (EBN) was 1st described in patients with Carney complex, 49 sporadic EBN cases, including 4 congenital EBN, have been reported. We present a 2-month-old healthy female with more than 1000 congenital EBN on the entire body. Skin biopsy revealed many nevus nests located in the upper dennis and a few nests extended around the sweat ducts and hair follicles in the middle and lower dermis. The heavily pigmented melanocytes were substantially epithelioid and occasionally spindle cells, admixed with melanophages. Immunohistochemistry revealed strong staining for S100 and HMB-45 but weak or moderate staining for Melan-A in dermal melanocytes after melanin bleaching with potassium permanganate and oxalic acid prior to incubation with the primary antibody. A diagnosis of congenital EBN was made based on clinicopathologic and immunopathologic findings. [ABSTRACT FROM AUTHOR]

The three-dimensional tubular heat exchanger has excellent performance in single-phase flow, but its performance in gas solid two-phase flow is still lacking research. In this paper, analysis and comparison of heat exchanger prototypes made up of four different three-dimensional tubes by different comprehensive performance evaluation methods are carried out through experimental research and theoretical analysis. The research results show that the ratio of the long axis to the short axis of the three-dimensional tube has an important influence on its heat transfer performance and flow resistance. Furthermore, a reasonable ratio can be obtained by comparing its comprehensive performance. Among the comprehensive performance analysis results of four kinds of three-dimensional tubes, the comprehensive performance factor ηo-I of type I is the best which achieves the purpose of heat transfer enhancement. Results from the current research could provide beneficial guidance for further design and application of the three-dimensional tubular heat exchanger.

Investigates the expression patterns of cyclooxygenases and prostaglandin E synthases in the endometrium of the rhesus monkey during the menstrual cycle. Result of immunostaining; Result of the coupling of cytosolic prostaglandin synthase and cyclooxygenase 1 in the luminal epithelium; Contribution of the coupling to the preparation of the receptive endometrium.

Driving emotion plays an important role in intention generation and behavior decision. It is an important premise to understand the influencing mechanism of driving emotion on intention correctly for the realization of driving intention identification, active vehicle security warning, and intelligent driving. In the case of two-lane, multi-source dynamic data of human–vehicle–environment under drivers’ different emotional states is obtained through actual and virtual driving experiment in this article. Considering the influence of emotion on intention, the probability distributions of the driving intention in different emotions are obtained by means of the immune algorithm. And the priming effect of vehicle driving emotion on intention was analyzed deeply. The research results can provide theoretical foundation for the research of driving intention identification, active vehicle security warning system, and intelligent driving command system under the condition of Internet of things.

Accurate identification of driving intention and reasonable control of driver’s behavior is seen as an important mean to reduce man-made traffic accidents for the intelligent vehicle. However, the intention identification processes associated with driving emotion-related impact have received very little attention. With the aim of uncovering the emotional impact on driving intention identification, the car-following condition was taken as an example, and multi-source and dynamic data of human–vehicle–environment under different driving emotional states were obtained through the experiments of emotions induction, actual driving, and virtual driving in this study. The feature extraction and dynamic identification models based on rough set theory and back-propagation artificial neural network were built to recognize driving intentions. The results showed that there were some differences in driving intention identification under different emotional modes. The differences were mainly manifested in the complexity and accuracy of intention feature vectors. The rationality and validity of the intentions feature extraction and identification models were verified by the actual driving experiments, virtual driving experiments, and interactive simulation experiments. The research results can provide theoretic basis for the emotional intelligence research of advanced vehicle, driving assist systems, and unmanned vehicles.

Anxiety is a common emotion of driver, which always affects the safety of driving. Eye movement characteristics can be used to understand the true emotion state of human beings. It is of great significance to study the law of eye movement for realizing active vehicle safety warning and human–machine cooperation. In this article, anxiety-induction experiment, real-vehicle driving experiments, and virtual driving experiments were designed and used to obtain the eye movement data of female novice extroversion driver under calm and anxiety, and mathematical statistics analysis was made on the fixation count, fixation duration, and visit duration in the area of interest within the driver horizon. The results showed that there are significant differences in fixation count and fixation duration of drivers ( p < 0 . 05 , p is the accompanying probability), and the main effect of emotion is significant ( p < 0 . 05 ) .Compared with the situation of calm, fixation area, fixation count, and fixation duration of drivers under anxiety were more focused on the middle area, the fixation count and visit duration on the left area were relatively more, the fixation duration on the right area was relatively longer, and anxiety was more likely to cause driver’s attention bias.

The article describes the bimetallic compound catena-Poly[hexaaquamagnesium(II)[bis(μ3-5-nitro-2-oxidoisophthalato)-dicopper(II)]dihydrate] which is the first metal-based supramolecular framework. The multifunctional linker used to construct this compound is the 5-nitrobenzene-1,2,3-tricarboxylic acid. This novel metal-organic complex was part of the research on the design and construction of functional metal-organic coordination polymers which can be applied in molecular absorption, heterogeneous catalysis and ion exchange.

Background: We aimed to determine whether early or delayed wheel-running (W) after a cortical lesion in rats influences functional recovery and protein expression involving synaptic plasticity.

In the title compound, [Cu(C(17)H(15)NO(3))(C(3)H(4)N(2))], the Cu(II) atom is four-coordinated by two O atoms and the N atom of the tridentate Schiff base ligand, and one N atom from the imidazole ligand in a distorted square-planar geometry. In the crystal structure, mol-ecules are linked into dimers by inter-molecular N-H⋯O hydrogen bonds.