251. Accumulation of element Ti in macrophage-like RAW264 cells cultured in medium with 1 ppm Ti and effects on cell viability, SOD production and TNF-alpha secretion.
- Author
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Taira M, Sasaki K, Saitoh S, Nezu T, Sasaki M, Kimura S, Terasaki K, Sera K, Narushima T, and Araki Y
- Subjects
- Animals, Cell Line, Cell Survival drug effects, Culture Media, Conditioned, Macrophages metabolism, Mice, Oxidative Stress, Spectrometry, X-Ray Emission, Titanium analysis, Macrophages drug effects, Superoxide Dismutase biosynthesis, Titanium toxicity, Tumor Necrosis Factor-alpha metabolism
- Abstract
The adverse effect of Ti on body-defense macrophage is not well understood. The aims of this study were twofold: (1) to examine the intracellular accumulation of Ti element; and (2) to measure the cell viability, superoxide dismutase (SOD) production, and TNF-alpha secretion of macrophage-like RAW264 cells cultured for two days in medium with 1 ppm Ti prepared from acidic ICP Ti standard solution. PIXE analysis showed that element Ti was accumulated up to 7.3 ppm in RAW264 cells when cultured in the medium with 1 ppm Ti. Further, RAW264 cells cultured in the medium with 1 ppm Ti exhibited cell viability of about 60%, SOD production of about 180%, and TNF-alpha secretion of about 170% relative to those of control cells cultured in the medium without Ti. It was speculated that phagocytosis of minute Ti-containing complex (mostly TiO2) by macrophage caused oxidative stress and inflammatory reaction, leading to cell proliferation arrest and increased production of SOD and TNF-alpha.
- Published
- 2006
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