151. Virus Infection Activates Thyroid Stimulating Hormone Synthesis in Intestinal Epithelial Cells
- Author
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Shaun Varghese, Bryce H. Vincent, Dina Montufar-Solis, and John R. Klein
- Subjects
medicine.medical_specialty ,endocrine system ,endocrine system diseases ,Thyrotropin, beta Subunit ,Biology ,Biochemistry ,Virus ,Article ,Cell Line ,Mice ,Downregulation and upregulation ,Thyroid-stimulating hormone ,In vivo ,Internal medicine ,medicine ,Animals ,Molecular Biology ,Mammalian orthoreovirus 3 ,Epithelial Cells ,Cell Biology ,Molecular biology ,Epithelium ,Small intestine ,Up-Regulation ,Intestines ,medicine.anatomical_structure ,Endocrinology ,Cell culture ,Sequence Analysis ,Hormone - Abstract
The small intestine has been shown to be an extra-pituitary site of thyroid stimulating hormone (TSH) production, and previous in vivo studies have shown that TSH synthesis localizes within areas of enteric virus infection within the small intestine; however, the cellular source of intestinal TSH has not been adequately determined. In the present study, we have used the murine MODE-K small intestinal epithelial cell line to demonstrate both at the transcriptional level and as a secreted hormone, as measured in a TSHbeta-specific enzyme-linked assay, that epithelial cells in fact respond to infection with reovirus serotype 3 Dearing strain by upregulating TSH synthesis. Moreover, sequence analysis of a PCR-amplified TSHbeta product from MODE-K cells revealed homology to mouse pituitary TSHbeta. These findings have direct functional implications for understanding a TSH immune-endocrine circuit in the small intestine.
- Published
- 2008