151. The 106b∼25 microRNA cluster is essential for neovascularization after hindlimb ischaemia in mice
- Author
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Yakov Krelin, Jonathan Semo, Jacob George, Michal Entin-Meer, David Kain, Arnon Afek, Sofia Maysel-Auslender, Gad Keren, Iris Barshack, Camila Avivi, and Rinat Sharir
- Subjects
Stromal cell ,Cell Survival ,Angiogenesis ,Neovascularization, Physiologic ,Apoptosis ,Bone Marrow Cells ,Hindlimb ,Neovascularization ,Antigens, CD ,Cell Movement ,Ischemia ,Paracrine Communication ,microRNA ,Animals ,Antigens, Ly ,Medicine ,AC133 Antigen ,Aorta ,Cell Proliferation ,Glycoproteins ,Mice, Knockout ,Tube formation ,Matrigel ,business.industry ,PTEN Phosphohydrolase ,Endothelial Cells ,Membrane Proteins ,Vascular Endothelial Growth Factor Receptor-2 ,Cell biology ,MicroRNAs ,Proto-Oncogene Proteins c-kit ,Immunology ,Female ,Cytokine secretion ,Stromal Cells ,medicine.symptom ,Peptides ,Cardiology and Cardiovascular Medicine ,business ,Blood Flow Velocity - Abstract
MicroRNAs (miRNAs, miR) are endogenous short RNA sequences that regulate a wide range of physiological and pathophysiological processes. Several miRNAs control the formation of new blood vessels either by increasing or by inhibiting angiogenesis. Here, we investigated the possible role of the miR-106b� 25 cluster in postnatal neovas- cularization and in regulation of the angiogenic properties of adult bone marrow-derived stromal cells. Methods and results To study the effect of miR-106b� 25 deletion on neovascularization, we used a miR-106b� 25 knockout (KO) mouse model. After inducing hindlimb ischaemia, we showed that vascularization in ischaemic mice devoid of miR-106b� 25 is impaired, as evident from the reduced blood flow on laser Doppler perfusion imaging. The miR-106b� 25 cluster was also shown here to be an essential player in the proper functioning of bone marrow-derived stromal cells through its regulation of apoptosis, matrigel tube formation capacity, cytokine secretion, and expression of the stem-cell marker Sca-1. In addition, we showed that capillary sprouting from miR-106b� 25 KO aortic rings is diminished. Conclusion These results show that the miR-106b� 25 cluster regulates post-ischaemic neovascularization in mice, and that it does so in part by regulating the function of angiogenic bone marrow-derived stromal cells and of endothelial cells.
- Published
- 2013