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Copyright of Applied Chemical Industry is the property of Shaanxi Research Design Insitute of Petroleum & Chemical Industry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Machines & Control / Dianji Yu Kongzhi Xuebao is the property of Electric Machines & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the value of preoperative differential diagnosis of triple-negative and non-triple-negative breast cancer based on multi-parameter MRI radiomics features. Methods The multi-parameter MRI of 106 breast cancer patients was retrospectively analyzed. 73 cases were in the training group and 33 cases were in the verification group. According to the pathological molecular classification, the patients were divided. into triple-negative (24) and non-triple negative (82) groups. ITK software was used to manually outline the volume of interest (VOI) of the lesion. and the imaging radiomics features were extracted and screened in A.K software to construct radiomics labels. Multiple logistic regression analysis was used to construct 4 prediction models of apparent diffusion coefficient (ADC), '12-weighted imaging (12WI), dynamic contrast enhancement (DCE), and combined feature sets. Using the area under the receiver operating characteristic curve (AUC) value, the sensitivity and specificity of the radiomics labels in the differential diagnosis of triple-negative breast cancer were calculated in the training group and verification group. Results In the training group) the AUC value of T2WI (0.993) was higher than that of ADC (0.964), DCE (0.866), and combined (0.973) feature sets. In the validation group, the AUC value of T2WI (0.885) was higher than that of ADC (0.837), DCE (0.810), and combined (0.880) feature sets. The sensitivities of T2WI and DCE feature sets were up to 96%. The specificity of ADC feature set was highest at 81.5%. Conclusion MRI radiomics features are valuable in distinguishing triple-negative and non-triple-negative breast cancer. The radiomics label based on T2WI feature has highest sensitivity and may be a potential biological marker. [ABSTRACT FROM AUTHOR]

Copyright of Electric Power Automation Equipment / Dianli Zidonghua Shebei is the property of Electric Power Automation Equipment Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Functional Materials / Gongneng Cailiao is the property of Chongqing Functional Materials Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective Long non-coding RNA （lncRNA） microarray was used to detect mRNA and lncRNA in Huh7 cells transfected with HBx， and the differentially expressed mRNAs and lncRNAs were screened out and their functions were analyzed. Methods Huh7 cells were cultured and divided into the wild-type HBx （HBx-FJ） group and pcDNA3. 1 group， which were transfected with pcDNA3. 1-HBx-FJ or pcDNA3. 1，respectively. The expression profiles of mRNA and lncRNA of Huh7 cells in the two groups were detected by lncRNA microarray. Differentially expressed genes were deter⁃ mined by the following conditions： the fold change was equal to or greater than 2. 0，the difference between the two groups was statistically significant （P<0. 05）and the false discovery rate was less than 0. 05. The differentially expressed mRNAs and lncRNAs were analyzed by Scatter plot and hierarchical cluster analysis， and the involvement of differentially ex⁃ pressed mRNAs in the biological process was discussed by combining the GO analysis and KEGG pathway analysis. Six key mRNAs were selected for RT-qPCR detection in HBx-transfected Huh7 cells and were compared with the microarray detection results. Results Compared with the pcDNA3. 1 group， there were 253 up-regulated mRNAs and 321 downregulated mRNAs，1 108 up-regulated lncRNAs and 1 182 down-regulated lncRNAs in the HBx-FJ group. Scatter plot and hierarchical cluster analysis showed that there were differentially expressed mRNAs and lncRNAs in the HBx-FJ group. GO and KEGG pathway analysis showed that the differentially expressed mRNAs were mainly involved in ion channel， transmembrane transport， antigen processing and presentation， regulation of protein processing and transcription⁃ al misregulation， signal transduction， and calcium signaling pathway， etc. Six key genes（ASAP3，CFTR，POSTN，CAM⁃ TA1，ESRRG，and ROBO1）in HBx-transfected Huh7 cells were detected by RT-qPCR， and the results were basically consistent with the microarray variation trend. Conclusions A large number of differentially expressed mRNAs and lncRNAs are found in HBx-transfected hepatocellular carcinoma Huh7 cells. ASAP3，CFTR，POSTN，CAMTA1，ESRRG， and ROBO1 may be involved in the regulation of HCC by HBx and play an important role. They are mainly involved in the processing of ion channel， transmembrane transport， antigen processing and presentation， regulation of protein， as well as the pathways of transcriptional misregulation， signal transduction， and calcium signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tropical Meteorology (1004-4965) is the property of Guangzhou Institute of Tropical & Oceanic Meteorology, China Meteorological Administration and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Chinese Mass Spectrometry Society is the property of Journal of Chinese Mass Spectrometry Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To investigate drug application in Beijing Children's Hospital Affiliated to Capital Medical University (hereinafter referred to as "our hospital"), so as to provide references for delicacy drug management. METHODS: Activity based classification (ABC) analysis, vital-essential-nonessential (VEN) analysis and ABC-VEN matrix analysis methods were adopted to analyze the varieties and consumption sum of drugs used by outpatient and inpatient in our hospital from Sept. 2018 to Aug. 2019, inventory turnover analysis and dosage analysis were conducted at the same time. RESULTS: Totally 698 kinds of drugs were used in the outpatient and inpatient in our hospital from Sept. 2018 to Aug. 2019, with the consumption sum of 669 322 100 yuan. Based on ABC analysis, the constitute ratio of varieties of drugs in categories A, B and C were respectively 15. 90% (111 kinds), 20. 20% (141 kinds) and 63. 90% (446 kinds), the constitute ratio of consumption sum were respectively 79. 85% (534 441 300 yuan), 15. 10% (101 081 400 yuan) and 5. 05% (33 799 400 yuan). Based on VEN analysis, the constitute ratio of varieties of drugs in categories V, E and N were respectively 35. 10% (245 kinds), 54. 44% (380 kinds) and 10. 46% (73 kinds), the constitute ratio of consumption sum were respectively 29. 30% (196 112 200 yuan), 63. 82% (427 185 900 yuan) and 6. 88% (46 024 000 yuan). Combined with ABC-VEN matrix analysis, the highest constituent ratio of varieties was 31. 52% (220 kinds) for drugs in CE category, the highest constituent ratio of consumption sum was 51. 76% (346 472 000 yuan) for drugs in AE category, the lowest constituent ratio of varieties was 0. 86% (6 kinds) for drugs in AN category, and the lowest constituent ratio of consumption sum was 0. 66% (4 411 800 yuan) for drugs in CN category. CONCLUSIONS: The upper and lower limits of inventory of partial CV-category and CE-category drugs should be appropriately adjusted to ensure the supply for clinical necessary and reduce the occurrence of drug shortage; there are some key drugs under national control among AE-category and AN-category drugs, reducing their use can not only reduce drug costs, but also help promoting clinical rational medication. Combining ABC analysis with VEN analysis to analyze the drug application in hospitals over a period of time is a scientific method applied to practical work, which can provide data support for the detailed management of drug inventory and clinical rational medication. [ABSTRACT FROM AUTHOR]

The development efficiency of the real estate industry is defined as the relative effective degree of transformation between input resources factors (land and capital) and output factors of the real estate development industry. The enhanced efficient DEA model is used to measure and analyze the development efficiency of Shenyang real estate industry by selecting the purchased land area and real estate development investment as the input indicators and the commercial housing sales area, commercial housing sales and land value-added tax as the output indicators. The results show that the development efficiency of Shenyang real estate industry is relatively low. Therefore, countermeasures and suggestions to optimize the development efficiency are put forward from the perspective of policies and enterprises in order to promote the transformation and upgrading of the industry. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Computer Engineering & Applications is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bearing is the property of Bearing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Although clinical studies have found that autologous adipose mesenchymal stem cells can effectively reduce facial fibrosis in patients with radiation-induced systemic sclerosis, but the mechanism of action has not been thoroughly analyzed. OBJECTIVE: To study the mechanism of action of adipose mesenchymal stem cells on bleomycin-induced systemic sclerosis in mice. METHODS: Forty SPF C57BL/6J female mice aged 6-8 weeks were randomly assigned to normal control group, adipose mesenchymal stem cells group, bleomycin group, and PBS group. Mice in the latter three groups were subjected to subcutaneous injection with bleomycin every other day for 28 days, and mouse models of systemic sclerosis were established. After successful model establishment, mice in the adipose mesenchymal stem cells group were subcutaneously injected with adipose mesenchymal stem cells; mice in the PBS group were subcutaneously injected with PBS; the treatments lasted for 14 days. Enzyme-linked immunosorbent assay was used to determine the levels of serum interleukin-17, transforming growth factor-β, interleukin-6, and tumor necrosis factor-α. Hematoxylin-eosin staining and Masson staining were utilized to measure histopathological changes in the skin and lung of systemic sclerosis mice. Immunofluorescence method was applied to examine collagen I, III, and V and CD31 expression levels in the skin and lung. RESULTS AND CONCLUSION: (1) Compared with the bleomycin group, the expression levels of interleukin-17, transforming growth factor-β, interleukin-6, and tumor necrosis factor-α were significantly decreased in the adipose mesenchymal stem cells group (P < 0.01). (2) Compared with the normal control group, the skin dermis of mice was thickened; inflammatory cells infiltrated; skin appendages reduced; the alveoli were atrophic and collapsed; with a lot of inflammatory cell infiltration, pulmonary arteriole wall thickening, microvascular basement membrane thickening, and fibrinoid necrosis, and the inflammatory symptoms improved after treatment in the adipose mesenchymal stem cells group. (3) Compared with the normal control group, the skin and lung tissues of bleomycin group mice showed a large aggregation of collagen fibers, and the collagen fibers were reduced after adipose mesenchymal stem cells treatment. (4) After treatment with adipose mesenchymal stem cells, the expression levels of collagen I, III, and V were decreased in the skin and lung tissue of mice, but the expression of CD31 in the skin tissues was increased in the bleomycin group (P < 0.01). (5) The results suggested that adipose mesenchymal stem cells can regulate the immune response of bleomycin mice and reduce fibrosis, inflammation and vascular lesions. [ABSTRACT FROM AUTHOR]

Objective To investigate the differential expression of PDK2 mediated by natural deletion mutant hepati‑ tis B virus X (HBx) protein in hepatocellular carcinoma (HCC), as well as the mechanism of long non-coding RNA (lncRNA) involved in regulation. Methods Huh7 cells were randomly divided into the empty vector group and HBx-128w group, and transfected with pcDNA3. 1 and pcDNA3. 1-HBx-128w, respectively. Western blotting was used to detect the expression of HBx protein in each group. LncRNA microarray was used to detect the stable transfected cells in the two groups, and the mRNA expression profiles and lncRNA expression profiles of Huh7 cells transfected with pcDNA3. 1-HBx128w were determined, and the differentially expressed genes were screened out. RT-qPCR was used to verify the results of lncRNA microarray in stable transfected cells. Bioinformatics was used to predict lncRNAs that might be involved in regulation, and RT-qPCR was used for verification in stable transfected cells. Results The lncRNA microarray results showed that PDK2 was up-regulated in Huh7 cells of HBx-128w group, and the fold change was 3. 219. RT-qPCR results showed that the concentration of PDK2 in Huh7 cells in the HBx-128w group was significantly higher than that in the pcDNA3. 1 group (P< 0. 05), and the fold change between the two groups was 3. 591. The expression level of lncRNA ENST0000511361 in HBx128w group was significantly higher than that in the pcDNA3.1 group (P<0. 05), and the fold change between the two groups was 3. 742. Conclusion PDK2 is up-regulated in Huh7 cells transfected with natural deletion mutant HBx-128w, and LncRNA ENST0000511361 may be involved in the regulation of PDK2 expression in the development of HCC. [ABSTRACT FROM AUTHOR]

Copyright of China Sciencepaper is the property of China Sciencepaper and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The Mohr-Wedge (MW) strength criterion is a theoretical formula， which is derived based on the rock wedge failure mechanics model and the partial differential equation related to the principal stresses. The MW criterion can be easily understood by using the dimensional analysis to arrange the multiple parameters with corresponding physical meanings. To analyze the applicability of the MW criterion including the rock critical state， the Mohr-Coulomb criterion， the Hoek-Brown criterion and the exponential criterion were selected as the comparison objects， and the average absolute error MAE was used as the evaluation index in order to quantitatively compare the accuracy of the four criteria in predicting rock strength. The results show that when the confining pressure is less than the rock critical state confining pressure σ○cr-M， the MW criterion has certain applicability. When the confining pressure is greater than σ○cr-M， the rock strength predicted by the four criteria is different due to the different rock critical state confining pressure. Therefore， a method to predict rock strength under different rock critical state confining pressures was proposed， which can provide some reference for choosing the value of rock critical state confining pressure. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Fuzhou University is the property of Journal of Fuzhou University, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bearing is the property of Bearing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Clinical Hepatology / Linchuang Gandanbing Zazhi is the property of Journal of Clinical Hepatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Mine Automation is the property of Industry & Mine Automation Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Frontiers of Computer Science & Technology is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the clinical effect and adverse reactions of budesonide in the treatment of bronchopulmonary dysplasia （BPD） in extremely premature infants （with gestational age at birth of less than 28 weeks）. Methods We retrospectively analyzed the treatment of 46 extremely premature infants with BPD， including the inhalation group of 20 cases and control group of 26 cases. The infants of inhalation group were treated with aerosol inhalation of 0. 5 mg budesonide， from the 28th day after birth， twice a day， for 10 days. The infants of the control group were not treated with budesonide or other types of glucocorticoids. Then the ventilator parameters of both on the 28th day and the 38th day after birth were compared between the two groups， including peak inspiratory pressure （PIP），mean airway pressure （MAP），fraction of inspired oxygen（FiO2 ），and arterial partial pressure of carbon dioxide （PCO2 ）. The duration of ventilation time， inhale oxygen time and hospitalization time were compared between the two groups to determine the treatment effects. The severity of BPD and adverse reactions were also compared between the two groups at the postmenstrual age of 36 weeks. Results After budesonide inhalation， the ventilator parameters on the 38th day after birth were significantly lower than those of the control group， including PIP， MAP， FiO2 and PCO2 （all P<0. 05）. There were 8 mild BPD cases，9 moderate BPD cases and 3 severe BPD cases in the inhalation group， while there were 3，12 and 11 cases in the control group. Compared with the control group， the proportion of mild BPD cases was higher and the proportion of severe cases was lower in the inhalation group （all P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the two groups （P>0.05）. The follow-up results of body weight， body length， head circumference and Gesell scores showed no statistically difference between the two groups at the postmenstrual age of 36 weeks and 6 months （all P>0. 05）. Conclusion The inhalation of budesonide in the treatment of extremely preterm infants with BPD can shorten the duration of ventilator time and oxygen supply time， reduce the proportion of infants with severe BPD， and meanwhile， it has good security. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Control Theory & Applications / Kongzhi Lilun Yu Yinyong is the property of Editorial Department of Control Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Power Automation Equipment / Dianli Zidonghua Shebei is the property of Electric Power Automation Equipment Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effects of wild-type and natural deletion mutant hepatitis B virus X (HBx) protein on the biological behavior of hepatocellular carcinoma (HCC) cells SMMC-7721. Methods HCC cell line SMMC 7721 was taken for routine culture and passage. SMMC 7721 cells in the logarithmic phase with good condition were randomly divided into the empty vector group, HBX-FJ group and HBX-128w group, which were transfected with pcDNA3 1, pcDNA3.1-HBX-FJ, and pcDNA3.1-HBX-128w, respectively. Western blotting was used to detect the expression of HBx protein in each group. The cell proliferation activity was detected by MTT assay. The cell proliferation ability was detected by Flat-plate colony assay. The cell cycle changes were detected by flow cytometry. The cell migration ability was detected by Scratch assay. The cell invasion ability was detected by Transwell assay. The tumorigenic ability in vitro was detected by tumorigenesis assay in nude mice. Results The proliferation activity, proliferation ability, invasion ability, and tumorigenic ability of cells in the HBX-FJ group and HBX-128w group were significantly higher than those in the empty vector group (all P < 0. 05). The proliferation activity, proliferation ability, migration ability, invasion ability and tumorigenic ability in vitro of cells in the HBx-128w group were significantly higher than those in the HBX-FJ group (all P < 0. 05). Compared with the empty vector group, the cells in the G1 phase decreased significantly while the cells in the S phase increased significantly in the HBX-FJ group and HBX-128w group (all P < 0. 05). Conclusion Both wild-type and natural deletion mutant HBx protein can promote the process of cell malignancy, but natural deletion mutant HBx-128w has a stronger carcinogenic effect than wild-type HBx; HBx can affect its biological function by deletion mutation and thus promote the progression of HCC. [ABSTRACT FROM AUTHOR]

Objective: Multifunctional Mn3O4@CuS core-shell nanoparticles were designed and prepared for imaging guided phototherapy of tumor, in order to achieve localized tumor photoablation with near infrared irradiation under the guidance of contrast enhanced magnetic resonance imaging (MRI). Methods: (1) Oleylamine-stabilized Mn3O4 nanoparticles were synthesized using thermal decomposition method, followed by surface coating of CuS nanoshell and PEGylation, resulting in water-solubleMn3O4@CuS core-shell nanoparticles. (2) Transmission electron microscope (TEM) morphology, UV-vis-NIR absorption spectra, size distribution and other physical-chemical properties of Mn3O4@CuS core-shell nanoparticles were evaluated, as well as its performance in MRI contrast enhanced and photo-induced hyperthermia. Results: Mn3O4@CuS core-shell nanoparticles had uniform spherical morphology with average size of 9.30±2.29 nm, and UV-vis-NIR absorption spectra showed their obvious and strong absorption in the near infrared region with peak absorbance at the range of 1100~1200 nm; The longitudinal relaxation rate (r1) of Mn3O4@CuS nanoparticles was calculated to be 1.662 mM-1 s-1, indicating the potential to act as a T1 MRI contrast agent; The temperature of Mn3O4@CuS nanoparticle suspension can be heated up to 73.5 ℃ with 785 nm near infrared laser irradiation, showing great capability to achieve photo-induced hyperthermia. Conclusion: Water-soluble Mn3O4@CuS core-shell nanoparticles were successfully fabricated with preferable MRI contrast enhancement and great photothermal effect, which could be applied for MRI guided phototherapy of tumor. [ABSTRACT FROM AUTHOR]