3,461 results on '"Y. Naito"'
Search Results
102. SS-MIX: a ministry project to promote standardized healthcare information exchange
- Author
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N. Fujita, T. Shimizu, T. Numano, H. Jozaki, Tadayoshi Fujiki, K. Toyoda, T. Nakashima, K. Nakayasu, F. Sasaki, Michio Kimura, Shigeki Tani, Yozo Ohshima, Takaya Sakusabe, K. Kawaguchi, M. Shimomura, Hiroshi Watanabe, H. Hoshi, Y. Naito, and Naoki Nakashima
- Subjects
Advanced and Specialized Nursing ,business.industry ,Proprietary format ,Legacy system ,Health Informatics ,World Wide Web ,Engineering management ,DICOM ,Software ,Health Information Management ,Japan ,Health care ,Information system ,Hospital Information Systems ,Medicine ,Humans ,Medical Record Linkage ,Cooperative Behavior ,Program Development ,business ,Information exchange ,Progress note - Abstract
Summary Objectives: To promote healthcare information exchange between providers and to allow hospital information systems (HIS) export information in standardized format (HL7 and DICOM) in an environment of widespread legacy systems, which only can export data in proprietary format. Methods: Through the Shizuoka prefecture EMR project in 2004–2005, followed by the ministry’s SS-MIX project, many software products have been provided, which consist of 1) a standardized storage to receive HL7 v2.5 mes sages of patient demographics, prescription orders, laboratory results, and diagnostic disease in ICD-10, 2) a referral letter creation system, 3) a formatted document creation system, 4) a progress note/nursing record system, and 5) an archive/viewer to incorporate incoming healthcare data CD and allow users to view on HIS terminal. Meanwhile, other useful applications have been produced, such as adverse event reporting and clinical information retrieval. To achieve the above-mentioned objectives, these software products were created and propagated, because users can use these software products, provided that their HIS can export the above information to the standardized storage in HL7 v2.5 format. Results: In 20 hospitals of Japan, the standardized storage has been installed and some applications have been used. As major HIS vendors are shipping HIS with HL7 export function since 2007, HIS of 594 hospitals in Japan became capable of exporting data in HL7 v2.5 format (as of March 2010). Conclusions: In high CPOE installation rate (85% in 400+ bed hospitals), though most of them only capable of exporting data in proprietary format, prefecture and ministry projects were effective to promote healthcare information exchange between providers. The standardized storage became an infrastructure for many useful applications, and many hospitals started using them. Ministry designation of proposed healthcare standards was effective so as to allow vendors to conform their products, and users to install them.
- Published
- 2010
103. A sensitive enzymatic method (SK-013) for detection of Treponema denticola, Porphyromonas gingivals and Bacteroides forsythus in subgingival plaque samples
- Author
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Kizuku Seida, Satoru Yamada, Katsuji Okuda, Atsushi Saito, Kazuyuki Ishihara, and Y Naito
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Population ,Bleeding on probing ,Colony Count, Microbial ,Dental Plaque ,Fluorescent Antibody Technique ,Dental plaque ,Benzoylarginine-2-Naphthylamide ,Microbiology ,Endopeptidases ,medicine ,Bacteroides ,Humans ,Treponema ,Periodontitis ,education ,Porphyromonas gingivalis ,education.field_of_study ,biology ,Treponema denticola ,Clinical Enzyme Tests ,biology.organism_classification ,Capnocytophaga ,medicine.disease ,stomatognathic diseases ,Regression Analysis ,Periodontics ,medicine.symptom - Abstract
An enzymatic method, SK-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. This method was found to have specificity for Porphyromonas gingivalis, Treponema denticola, Bacteroides forsythus, and some Capnocytophaga strains. The purpose of this study was to determine whether SK-013 could indicate the presence of periodontopathic bacteria, including T. denticola, P. gingivalis and B. forsythus, which produce trypsin-like enzymes. Subgingival plaque samples were taken from 10 clinically healthy sites and 30 periodontally diseased sites with 3 paper points. SK-013 activity of plaque samples was assayed, and the numbers of T. denticola, P. gingivalis and B. forsythus in the sample were counted by immunofluorescence technique. In diseased sites, the SK-013 activity was significantly correlated with clinical parameters such as Gingival Index, Plaque Index, probing depth and bleeding on probing. A significant correlation was found between the presence of these organisms and SK-013 activity. Correlation coefficients between the presence of T. denticola and SK-013 activity were higher than those with other organisms. These findings indicate that the SK-013 is useful as an indicator of cell population of T. denticola, P. gingivalis and B. forsythus in subgingival plaque.
- Published
- 1992
104. Issues in evaluating joint range of motion and unique movements in synchronized swimmers
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Y. Kobayakawa, Kazuyuki Shinohara, Tadashi Wada, M. Takahashi, Hirofumi Jigami, Yuusuke Takahashi, D. Inoue, T. Matsumoto, and Y. Naito
- Subjects
business.industry ,Computer science ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,Computer vision ,Artificial intelligence ,Range of motion ,business ,Joint (geology) - Published
- 2013
105. On the properties of lossless reciprocal 4-port circuits with reflection symmetry
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Y. Naito and K. Araki
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Lossless compression ,Impedance matching ,Topology ,Computer Science::Hardware Architecture ,Computer Science::Emerging Technologies ,Reflection symmetry ,Control theory ,Transmission coefficient ,Electrical and Electronic Engineering ,Network synthesis filters ,Invariant (mathematics) ,Reciprocal ,Hardware_LOGICDESIGN ,Electronic circuit ,Mathematics - Abstract
An invariant for lossless reciprocal 4-port circuits with reflection symmetry is derived. It is shown that all these circuits can be transformed by external compensation into ideal 4-port circuits with perfect matching and isolation. The transmission coefficient and isolation ports are uniquely determined by the invariant. Furthermore, a design method for the external compensating circuits is described. >
- Published
- 1992
106. Impaired gastric ulcer healing in diabetic mice: role of methylglyoxal
- Author
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Y, Naito, T, Takagi, T, Oya-Ito, H, Okada, T, Suzuki, I, Hirata, M, Hirai, K, Uchiyama, O, Handa, K, Uchida, and T, Yoshikawa
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Blood Glucose ,Glycation End Products, Advanced ,Male ,Ornithine ,Wound Healing ,Glycosylation ,Proteins ,Anti-Ulcer Agents ,Pyruvaldehyde ,Severity of Illness Index ,Diabetes Mellitus, Experimental ,Mice, Inbred C57BL ,Mice ,Pyrimidines ,Gastric Mucosa ,Thiadiazoles ,Animals ,Regeneration ,Thiazolidines ,Stomach Ulcer ,Protein Processing, Post-Translational ,Peroxiredoxin VI - Abstract
Methylglyoxal is a reactive dicarbonyl compound produced from cellular glycolytic intermediates that reacts non-enzymatically with proteins to form products such as argpyrimidine at arginine residue. The aim of the present study was to investigate the role of methylglyoxal in the delayed healing of gastric ulcer in diabetes, and to identify the methylglyoxal-modified proteins as a target molecule of this modification. Using male C57BL/6 mice, diabetes was induced by a single i.p. injection of streptozotocin and gastric ulcers were produced by the focal application of 40% of acetic acid to the serosal surface of the stomach. In order to evaluate the effect of OPB-9195, an inhibitor of methylglyoxal modification, on gastric ulcer healing, mice were given orally OPB-9195 (30 mg/kg) twice daily for 14 days, one week before and after the injection of streptozotocin. The area of gastric ulcer on day 7 was significantly increased in diabetic mice compared to non-diabetic mice, indicating delayed ulcer healing. This increase in ulcer area in diabetic mice was significantly reversed by the treatment with OPB-9195 without affecting blood glucose levels. Proteomics analysis showed the methylglyoxal-modification of peroxiredoxin 6 proteins in the diabetic gastric mucosa around gastric ulcer, and this modification was markedly inhibited by the treatment with OPB-9195. In conclusion, the present study suggests a link of increased methylglyoxal modification of proteins including peroxiredoxin 6 to the delayed gastric ulcer healing in diabetes, and also shows the therapeutic potential of the inhibitor of methylglyoxal modification for the treatment of diabetic gastric ulcers.
- Published
- 2009
107. A 1/3-in 510(H)*492(V) CCD image sensor with mirror image function
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T. Wakayama, M. Itasaka, H. Mori, Naoki Kato, E. Komatsu, K. Foujikawa, J. Hojo, and Y. Naito
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Microlens ,Physics ,business.industry ,Hole accumulation diode ,Electronic, Optical and Magnetic Materials ,Reduction (complexity) ,Optics ,Optical format ,Electrical and Electronic Engineering ,Image sensor ,business ,Sensitivity (electronics) ,Shift register ,Dark current - Abstract
A 1/3-in optical format 510(H)*492(V) interline charge-coupled-device (CCD) image sensor with a mirror-image function has been developed. To realize both a normal image and a mirror image, the horizontal shift register (H-CCD) is transferred forward and backward by a four-electrode, quasi-two-phase clock drive. The unit cell size is 9.6(H)*7.5(V) mu m/sup 2/. An on-chip microlens has been developed to achieve a sensitivity of 28 mV/lx, which is higher than that of the conventional 1/2-in device. The hole accumulation diode (HAD) sensor used has the advantage of low dark current, negligibly small lag, high blooming suppression, and a variable-speed electronic shutter. The smear reduction level is -83 dB. Horizontal resolution of 330 TV lines is obtained. >
- Published
- 1991
108. Robot consciousness and representation of facial expressions
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Y. Naito, K. Mori, and J. Takeno
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Facial expression ,Computer science ,business.industry ,media_common.quotation_subject ,Artificial consciousness ,Kansei ,Happiness ,Robot ,Computer vision ,Artificial intelligence ,Consciousness ,business ,Humanoid robot ,Stream of consciousness (psychology) ,media_common - Abstract
The humanoid robot we developed is capable of changing its facial expressions according to simple artificial consciousness and stream of consciousness. We think that consciousness consists basically of dasialanguage and its associative streampsila and dasiarepresentation of feelings related to the language.psila The robot named Kansei, a Japanese term referring to emotion, achieves linguistic association similar to a stream of consciousness and represents its feelings according to the associations through artificial consciousness. The robot is equipped with an artificial cranium made of aluminium which contains 19 servomotors inside it. For the surface of the face, we use polyurethane, a relatively soft material which creates a shape that is closely mimics a human face. Facial expressions are formed on the polyurethane by pulling it into shape via metal wires attached to the 19 internal servomotors. Besides the six basic facial expressions such as happiness and anger, the robot can also make complex expressions that include both happiness and fear.
- Published
- 2008
109. SUN-PP114: Preoperative Prediction of Pneumonia and Oral Intake after Percutaneous Endoscopic Gastrostomy (PEG)
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M. Kodama, S. Nishimura, T. Ishikawa, S. Kokura, Hideyuki Konishi, Y. Naito, and T. Nishimura
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medicine.medical_specialty ,Pneumonia ,Nutrition and Dietetics ,business.industry ,General surgery ,Percutaneous endoscopic gastrostomy ,medicine.medical_treatment ,PEG ratio ,medicine ,Critical Care and Intensive Care Medicine ,business ,medicine.disease ,Surgery - Published
- 2015
110. Fast-scanning atomic force microscopy reveals the molecular mechanism of DNA cleavage by ApaI endonuclease
- Author
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Toshio Ando, Akira Yagi, Y. Naito, Shige H. Yoshimura, Nobuaki Sakai, Masatoshi Yokokawa, and Kunio Takeyasu
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Models, Molecular ,Protein Conformation ,Materials Science (miscellaneous) ,Dimer ,Microscopy, Atomic Force ,DNA sequencing ,chemistry.chemical_compound ,Motion ,Protein structure ,Computer Systems ,Computer Simulation ,Electrical and Electronic Engineering ,Deoxyribonucleases, Type II Site-Specific ,chemistry.chemical_classification ,Binding Sites ,Protein dynamics ,DNA ,DNA-Binding Proteins ,Enzyme Activation ,Restriction enzyme ,Crystallography ,Monomer ,Enzyme ,chemistry ,Models, Chemical ,Control and Systems Engineering ,Nucleic Acid Conformation ,Protein Binding - Abstract
Newly developed fast-scanning atomic force microscopy (AFM) allows the dissection of molecular events such as DNA–enzyme reactions at the single-molecule level. With this novel technology, a model is proposed of the DNA cleavage reaction by a type IIP restriction endonuclease ApaI. Detailed analyses revealed that ApaI bound to DNA as a dimer and slid along DNA in a one-dimensional diffusion manner. When it encountered a specific DNA sequence, the enzyme halted for a moment to digest the DNA. Immediately after digestion, the ApaI dimer separated into two monomers, each of which remained on the DNA end and then dissociated from the DNA end. Thus, fast-scanning AFM is a powerful tool to aid the understanding of protein structures and dynamics in biological reactions at the single-molecule level in sub-seconds.
- Published
- 2006
111. DEA-based indicator for performance improvement
- Author
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Hiroshi Tsuji, S. Aoki, and Y. Naito
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Database normalization ,Set (abstract data type) ,Range (mathematics) ,Mathematical optimization ,Returns to scale ,Three-dimensional graph ,Computer science ,Data envelopment analysis ,Data mining ,Performance improvement ,Constant (mathematics) ,computer.software_genre ,computer - Abstract
This paper concerns an active indication method on data envelopment analysis and its experimental application. While DEA works to evaluate efficiency for the decision making unit with multiple input and output, there are still problems as follows: It is not easy (1) to view each DMU's position from bird-eye, (2) to catch the relationship between a DMU and its dominant set, and (3) to get an improvement direction from the current DMU position to the dominant set. To solve these problems, this paper clarifies the visualization procedure for indicating performance improvement and applies it to Japanese prefecture evaluation and verified in a two-input/single-output problem and a single-input/three-output problem as follows: (1) data normalization works well under the assumption that returns to scale is constant, (2) logarithmic transformation is useful in the case that the data range is strongly biased, and (3) viewpoint in three dimensional graph should be changed for confirming the direction.
- Published
- 2005
112. Application of Nanocrystalline Soft Magnetic Fe-M-B (M=Zr,Nb) Alloys 'nanoperm/sup TM/' to Choke Coils
- Author
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T. Hatanai, Y. Naito, A. Inoue, A. Makino, and T. Bitoh
- Subjects
Materials science ,Magnetic shape-memory alloy ,Permeability (electromagnetism) ,Choke ,Composite material ,Inductor ,Magnetic liquids ,Saturation (magnetic) ,Nanocrystalline material ,Amorphous solid - Published
- 2005
113. Interaction Of High-power Microwaves With Plasma In A Corrugated Wall Waveguide
- Author
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K. Minami, A. Sugawara, Y. Naito, K. Ogura, T. Watanabe, N. University, Y. Carmel, W.W. Destler, and V.L. Granatstein
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Materials science ,chemistry.chemical_element ,Plasma ,Grating ,law.invention ,Electric discharge in gases ,Standing wave ,chemistry ,Physics::Plasma Physics ,law ,Ionization ,Atomic physics ,Waveguide ,Helium ,Microwave - Abstract
Plasma filled backward wave oscillators (BWOs) utilizing IREBs have demonstrated the efficiency enhancement up to 40%. Botton and Ron suggested that the high efficiency could be explained by a self-induced distributed feedback mechanism of a density grating formed by ponderomotive potential of backward and forward waves in the slow wave structure. The authors carried out an experiment to detect such plasma grating formed by high-power externally injected microwaves. Weakly ionized plasma is produced by DC gas discharge (helium 52 mTorr, current f{sub p}. Such rises are not the results of additional RF ionization, but are believed to be new nonlinear phenomena inherent in corrugated waveguide. LP analysis shows that the rises in ISC consist of two reasons: Large local rises in plasma potential and small local rises in plasma density. The authors also carry out RF standing wave measurements on the axis. In conclusion, their experimental results are not completely identical to Botton and Ron`s prediction.« less
- Published
- 2005
114. A 1/3-INCH 360k PIXEL PROGRESSIVE SCAN CCD IMAGE SENSOR
- Author
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Y. Naito, A. Kobayashi, T. Ishigami, S. Nakagawa, Y. Shimohida, and A. Izumi
- Published
- 2005
115. Poststreptococcal acute glomerulonephritis superimposed on bilateral renal hypoplasia
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Y, Naito-Yoshida, Y, Naico-Yoshida, M, Hida, Y, Maruyama, N, Hori, and M, Awazu
- Subjects
Nephrology ,medicine.medical_specialty ,Streptococcus pyogenes ,Urology ,Renal function ,Nephron ,Urine ,Kidney ,Severity of Illness Index ,Diabetic nephropathy ,chemistry.chemical_compound ,Glomerulonephritis ,Internal medicine ,Streptococcal Infections ,medicine ,Humans ,Child ,Creatinine ,business.industry ,General Medicine ,medicine.disease ,Renal hypoplasia ,Surgery ,medicine.anatomical_structure ,chemistry ,Acute Disease ,Female ,business ,Kidney disease ,Follow-Up Studies - Abstract
An 8-year-old girl with preexisting chronic renal failure (CRF) due to bilateral renal hypoplasia presented with edema, gross hematuria and acute deterioration of renal function. The diagnosis of poststreptococcal acute glomerulonephritis (PSAGN) was made based on clinical presentation, red blood cell casts, low level of C3 and elevated antistreptolysin 0 titer. Her course was prolonged with serum creatinine increased from the baseline level of 1.1 mg/dl to 2.2 mg/dl, returning toward the baseline level (1.2 mg/dl) after one month. Serum creatinine then started to increase again. The slope of creatinine clearance over time became steeper after the episode of PSAGN. A severe course of PSAGN and subsequent deterioration of renal function have previously been reported in patients with diabetic nephropathy or focal glomerulosclerosis. The present case along with a literature review suggests that individuals with fewer nephrons are at higher risk of severe course and outcome of PSAGN. Conversely, patients with severe PSAGN may be born with fewer nephrons due to low birth weight, unrecognized renal hypoplasia or other unknown causes.
- Published
- 2005
116. Directly-Connected Image Guide 3db Coupler with Very Flat Coupling
- Author
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D. Kawabe, K. Araki, Dong Il Kim, and Y. Naito
- Subjects
Physics ,Optics ,business.industry ,Excited state ,Broadband ,Bandwidth (signal processing) ,Physics::Optics ,Power dividers and directional couplers ,Dielectric ,business - Abstract
Design method of the broadband, compact, and mechanically stable 3dB coupler with very flat coupling are demonstrated using a directly-connected image guide. The experimental results agree reasonably well with the theoretical ones by forming appropriate tapers even at frequencies where higher modes could be excited.
- Published
- 2005
117. Sidelobe control of aperture antenna by dielectric rim loading
- Author
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Y. Naito and Semo Chung
- Subjects
Optics ,Materials science ,Horn antenna ,Directional antenna ,business.industry ,Fresnel zone antenna ,Reflective array antenna ,Antenna aperture ,Dielectric loss ,Slot antenna ,Dielectric ,business - Published
- 2005
118. A Novel Energy Relation in Eigen Modes of Transmission Line and its Application to the Derivation of Variational Expression for Propagation Constant
- Author
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K. Araki and Y. Naito
- Subjects
Physics ,symbols.namesake ,Electric power transmission ,Maxwell's equations ,Condensed matter physics ,Transmission line ,Mathematical analysis ,symbols ,Telegrapher's equations ,Expression (computer science) ,Propagation constant ,Integral equation ,Energy (signal processing) - Abstract
A novel energy relation in eigen modes of transmission line is herein proposed and the variational express ions for propagation constant of various transmission lines, which have never been treated in variational form, can be systematically derived through this relation.
- Published
- 2005
119. Design of absorber for small anechoic chamber
- Author
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Y. Naito and S. Shibato
- Subjects
Optics ,Materials science ,Anechoic chamber ,business.industry ,Attenuation ,business - Published
- 2005
120. On ferrite bar antenna and its gain limitation
- Author
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S. Sato and Y. Naito
- Subjects
Materials science ,Nuclear magnetic resonance ,chemistry ,Permeability (electromagnetism) ,Demagnetizing field ,Ferrite (magnet) ,chemistry.chemical_element ,Composite material ,Hafnium - Published
- 2005
121. Dipole measurement in small anechoic chamber
- Author
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K. Horie and Y. Naito
- Subjects
Materials science ,Coaxial antenna ,Anechoic chamber ,business.industry ,Loop antenna ,Antenna measurement ,law.invention ,Radiation pattern ,Dipole ,Optics ,law ,Dipole antenna ,business ,Monopole antenna - Published
- 2005
122. Microwave Permeability Tensor of Partially Magnetized Ferrites
- Author
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Y. Naito and M. Igarashi
- Subjects
Magnetization ,Magnetic anisotropy ,Mu-metal ,Materials science ,Condensed matter physics ,Demagnetizing field ,Magnetic hysteresis ,Omega ,Magnetic susceptibility ,Saturation (magnetic) - Abstract
The microwave permeability tensor of partially magnetized ferrites is formularized. The theory clarifies that the remarkable low-field losses disappear if omega/sub M/ / omega
- Published
- 2005
123. New Edge Guided Wave on Ferrite Strip
- Author
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S. Enjouji, K. Araki, and Y. Naito
- Subjects
Optics ,Guided wave testing ,Materials science ,Physics::Instrumentation and Detectors ,business.industry ,Wave propagation ,Mode (statistics) ,Ferrite (magnet) ,Millimeter ,business ,Optical integrated circuit ,Microstrip ,Magnetic field - Abstract
Electromagnetic wave propagation along ferrite strip whose cross section is square immensed in D.C. magnetic field is investigated experimentaly. The existence of the mode similar to edge guided mode in ferrite substrate microstrip line was discovered. This mode may be useful for nonreciprocal circuit in millimeter and optical integrated circuit.
- Published
- 2005
124. Parallel Component mu/sub /spl Imped// of Partially Magnetized Microwave Ferrites
- Author
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M. Igarashi and Y. Naito
- Subjects
Physics ,Nuclear magnetic resonance ,Component (thermodynamics) ,Microwave permeability ,Tensor ,Microwave ,Physics::Geophysics ,Computational physics - Abstract
A formula for the parallel component mu/sub /spl imped// of the microwave permeability tensor in the partially magnetized state is derived. The theory is in good agreement with experimental results.
- Published
- 2005
125. Phase-Matched Waveguide Using the Artificial Anisotropic Structure and its Application to Mode Converter
- Author
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T. Mizumoto, H. Yamazaki, and Y. Naito
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Materials science ,business.industry ,Phase (waves) ,Mode (statistics) ,Physics::Optics ,Dielectric ,STRIPS ,Waveguide (optics) ,law.invention ,Planar ,Optics ,law ,Optical filter ,business ,Electrical conductor - Abstract
Phase-matching technique by the artificial anisotropic structure and its application to mode converter are proposed for the millimeter-wave dielectric circuitry. Phase-matched dielectric planar waveguide is designed and mode conversion characteristics are studied. Mode conversion experiment is successfully performed.
- Published
- 2005
126. New Nonreciprocal Devices in a Coplanar Waveguide
- Author
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Y. Yamanaka and Y. Naito
- Subjects
Materials science ,business.industry ,Coplanar waveguide ,Physics::Optics ,Integrated circuit ,law.invention ,law ,Electronic engineering ,Optoelectronics ,Ferrite (magnet) ,Astrophysics::Earth and Planetary Astrophysics ,business ,Microwave ,MathematicsofComputing_DISCRETEMATHEMATICS - Abstract
Some new nonreciprocal magnetic devices in a coplanar waveguide, using the edge-guided mode, is proposed. The structure of these devices are suitable for application to microwave integrated circuits, because of a plane configuration. Experimental resulats on some types of coplanar structures with ferrite material are described.
- Published
- 2005
127. Muscarinic acetylcholine receptor autoantibodies in patients with Sjögren's syndrome
- Author
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Takayuki Sumida, Ryutaro Matsumura, Daisuke Goto, Y Naito, Isao Matsumoto, Ei Wakamatsu, Satoshi Ito, Akito Tsutsumi, and T. Sugiyama
- Subjects
Autoimmune disease ,Letter ,Immunology ,T-cell receptor ,Autoantibody ,Biology ,medicine.disease ,medicine.disease_cause ,Receptors, Muscarinic ,General Biochemistry, Genetics and Molecular Biology ,Epitope ,Autoimmunity ,Sjogren's Syndrome ,stomatognathic system ,Rheumatology ,Matters Arising ,Muscarinic acetylcholine receptor ,medicine ,Immunology and Allergy ,Humans ,Receptor ,Acetylcholine receptor ,Autoantibodies - Abstract
Sjogren’s syndrome (SS) is an autoimmune disease characterised by lymphocytic infiltration into the lachrymal and salivary glands, leading to dry eyes and mouth. Infiltration is also found in the kidneys, lungs, thyroid, and liver. Immunohistochemical studies have shown that most infiltrating lymphocytes around the labial salivary and lachrymal glands, and kidneys are CD4 positive αβ T cells. Previous studies with polymerase chain reaction provide evidence about the T cell receptor Vβ and Vα genes on these T cells, and sequence analysis of the CDR3 region indicates some conserved amino acid motifs, supporting the notion that infiltrating T cells recognise relatively few epitopes on autoantigens.1 Candidate autoantigens recognised by T cells infiltrating the labial salivary glands of patients with SS have been analysed, and Ro/SSA 52 kDa, α-amylase, heat shock protein, and T cell receptor BV62 have been identified. However, there is no direct evidence that these reactive T cells really attack and destroy the salivary glands. In contrast, the presence of autoantibodies (Abs) against M3 muscarinic acetylcholine receptor (M3R) has been reported, and it is suggested that an immune reaction to M3R plays a crucial …
- Published
- 2005
128. [Ruptured syphilitic ascending aortic aneurysm; report of a case]
- Author
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Shinya, Yokoyama, Y, Naito, E, Koh, B, Ohta, and M, Kitamura
- Subjects
Blood Vessel Prosthesis Implantation ,Humans ,Female ,Aneurysm, Ruptured ,Emergencies ,Middle Aged ,Aorta ,Aortic Aneurysm ,Syphilis, Cardiovascular - Abstract
Syphilitic cardiovascular disease has become a rarity today. It most commonly affects the ascending aorta and aortic arch. A case is reported of a 50-year-old female with a ruptured syphilitic ascending aortic aneurysm who underwent an emergency operation. The patient was emergently admitted with complaint of severe dyspnea, circulatory shock due to cardiac tamponade was observed in the emergency room. Bentall's procedure was performed. Intraoperative findings showed multi-cystic dilatation of an aortic root. Penetration was existed on right side posterior wall of an aortic root. Postoperative pathological examination of the aneurysmal wall revealed the characteristic finding of syphilitic aortitis. The postoperative course was uneventful and the patient has been doing well at period of 3 years after operation.
- Published
- 2004
129. [Ischemic cardiomyopathy treated with overlapping ventriculoplasty]
- Author
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Yasuhisa, Fukada, Y, Matsui, Y, Naito, and K, Yasuda
- Subjects
Male ,Heart Ventricles ,Myocardial Ischemia ,Humans ,Mitral Valve ,Mitral Valve Insufficiency ,Coronary Disease ,Cardiac Surgical Procedures ,Coronary Artery Bypass ,Middle Aged ,Papillary Muscles - Abstract
A 58-year-old man was admitted due to congestive heart failure. Left ventriculography showed end-diastolic volume index (EDVI) of 172 ml/m2 and ejection fraction (EF) of 16%. Coronary angiography showed severe double vessel disease (the left anterior descending artery and the right coronary artery). Echocardiography showed dilated heart [left ventricular end-diastolic diameter (LVDd) of 74 mm] and severe mitral regurgitation due to tethering of the leaflets. The patient underwent our original left ventricular volume reduction operation, termed overlapping ventriculoplasty (OLVP) combined with mitral annuloplasty, papillary muscles plication (PMP) and coronary artery bypass grafting (CABG). The postoperative course was excellent. Postoperatively, the EDVI decreased to 96 ml/m2, the LVDd diminished to 67 mm, and the EF improved to 34%. This case implies the role of left ventricular remodeling procedure of OLVP and PMP in the ischemic cardiomyopathy with ischemic mitral regurgitation.
- Published
- 2004
130. [Primary cardiac malignant lymphoma diagnosed intraoperatively during aortic valve repair; report of a case]
- Author
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Shinya, Yokoyama, Y, Naito, I, Tei, E, Koh, A, Nagata, and G, Katoh
- Subjects
Heart Neoplasms ,Heart Valve Prosthesis Implantation ,Male ,Lymphoma ,Doxorubicin ,Aortic Valve ,Antineoplastic Combined Chemotherapy Protocols ,Aortic Valve Insufficiency ,Humans ,Cisplatin ,Middle Aged ,Combined Modality Therapy - Abstract
A 58-year-old male who complained of chest oppression on effort was diagnosed as aortic regurgitation by echocardiography. The cardiac catheterization was showed moderate aortic regurgitation. He was referred to our hospital for aortic valve replacement. We considered preoperatively that the aortic regurgitation is due to infective endocarditis or aortitis syndrome. We have not noticed cardiac tumor at all until aortic valve replacement. Postoperative pathological diagnosis of the tumor was malignant lymphoma. The tumor had a characteristic architecture of a cardiac MICE (mesothelial/monocytic incidental cardiac excrescence). After the operation, additional chemotherapy using doxorubicin and cysplatin was performed. The patient has been doing well for 5 years after operation.
- Published
- 2004
131. [RNA-interference, induced by transient and continuous expression of hairpin RNA in cells from Drosophila and mammals]
- Author
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K, Ui-Tei, R, Ueda, S, Zenno, F, Takahashi, N, Doi, Y, Naito, M, Yamamoto, N, Hashimoto, K, Takahashi, T, Hamada, T, Tokunaga, and K, Saigo
- Subjects
Base Sequence ,Animals ,Humans ,Drosophila ,RNA Interference ,DNA ,Promoter Regions, Genetic ,In Situ Hybridization ,Cell Line ,DNA Primers ,Plasmids ,RNA, Double-Stranded - Abstract
RNA interference (RNAi) may be induced by a plasmid with an inverted repeat (IR) sequence directing transcription of hairpin-type double-stranded RNA (dsRNA). This study examines the effects of changing various parameters of IR constructs on Drosophila and mammalian RNAi, using the dual luciferase system, RNAi activity was found to vary depending on IR length ass well as the length and sequence of the internal loop separating sense and antisense sequences. Both transient and stable RNAi occurred in Drosophila cultured cells. Although transient DNA-mediated RNAi was noted in most mammalian cells, no mammalian cells stably possessing IR sequences and hence RNAi activity could be obtained. In Drosophila, DNA-mediated RNAi was considerably weaker than long-dsRNA-mediated RNAi. The cytological data indicated that this was most probably caused by abortive processing of hairpin RNA produced within cells. DNA-mediated RNAi was examined at the level of Drosophila individuals using extramacrochaetae as a model gene, and the presence of an intron sequence in the single-stranded loop region was shown to be essential for effective RNAi.
- Published
- 2004
132. An improvement of matrix-assisted laser desorption/ionization mass spectrometry using an infrared tunable free electron laser
- Author
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Y NAITO
- Subjects
Nuclear and High Energy Physics ,Instrumentation - Published
- 2004
133. Effects of Kinect Sports on health indices of female university students
- Author
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T. Kobayashi and Y. Naito
- Subjects
Medical education ,medicine.medical_specialty ,business.industry ,Physical therapy ,Medicine ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,business - Published
- 2012
134. The effect of rebamipide on Helicobacter pylori extract-mediated changes of gene expression in gastric epithelial cells
- Author
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N, Yoshida, T, Ishikawa, E, Ichiishi, Y, Yoshida, K, Hanashiro, M, Kuchide, K, Uchiyama, S, Kokura, H, Ichikawa, Y, Naito, Y, Yamamura, T, Okanoue, and T, Yoshikawa
- Subjects
Alanine ,Helicobacter pylori ,Reverse Transcriptase Polymerase Chain Reaction ,Monokines ,Chemokine CXCL2 ,NF-kappa B ,Down-Regulation ,Gene Expression ,Epithelial Cells ,Quinolones ,Anti-Ulcer Agents ,Up-Regulation ,Gastric Mucosa ,Stomach Neoplasms ,Tumor Cells, Cultured ,Humans ,Fibroblast Growth Factor 2 ,RNA, Messenger ,Chemokine CCL5 ,Oligonucleotide Array Sequence Analysis - Abstract
Recent studies have shown that Helicobacter pylori affects intracellular signal transduction in host cells, leading to the activation of transcriptional factors and the induction of pro-inflammatory cytokines. On the other hand, rebamipide, an anti-gastritis and anti-ulcer agent, could scavenge reactive oxygen species and reduce interleukin-8 (IL-8) expression in gastric epithelial cells induced by H. pylori-stimulation through the attenuated activation of nuclear factor-kappaB (NF-kappaB).In this study, we investigated the effects of rebamipide on gene expression in H. pylori-stimulated epithelial cells using DNA chip.H. pylori water extract (HPE) was prepared from NCTC11637, the type strain of H. pylori. Total RNA was extracted from MKN45 cells, a human gastric cancer cell line, following HPE-stimulation with and without rebamipide for 3 h, and differences in gene expression profiles were observed using GeneChip and Human 6800 probe array.The GeneChip analysis demonstrated that 132 up-regulated genes and 873 down-regulated genes, such as growth factors, chemokines and transcription factors, were detected in MKN45 cells 3 h after stimulation of H. pylori. Among them, several genes, including bFGF, RANTES and MIP-2beta, were previously unknown to be expressed in H. pylori-stimulated human gastric cells. Rebamipide reduced expression of 119 genes encoding cytokines, growth factors and their receptors and transcription factors.These findings suggest that rebamipide could inhibit inflammatory reactions and tumour progression by modifying H. pylori infection-induced gene expression in gastric epithelial cells.
- Published
- 2003
135. Compression molding of biodegradable board using waste of bedding cotton
- Author
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T. Kimura, Y. Naito, and S. Hatta
- Published
- 2003
136. [Surgery for distal aortic arch aneurysm with a patent left internal mammary-to-coronary artery graft; report of a case]
- Author
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M, Nakajima, K, Tsuchiya, Y, Naito, N, Hibino, H, Matsumoto, and H, Inoue
- Subjects
Male ,Blood Vessel Prosthesis Implantation ,Postoperative Complications ,Aortic Aneurysm, Thoracic ,Humans ,Aorta, Thoracic ,Coronary Artery Bypass ,Middle Aged ,Internal Mammary-Coronary Artery Anastomosis - Abstract
We report a case of 62-year-old male who suffered from a distal aortic arch aneurysm developed 5 years after coronary artery bypass grafting (CABG). Preoperative angiography revealed a distal arch aneurysm and a patent left internal mammary artery (LIMA) graft. Graft replacement of the total aortic arch was performed using a 4 branched graft. After the re-median sternotomy, cardiopulmonary bypass was established with ascending aortic perfusion and right atrium (RA) drainage. Myocardial protection was achieved with root cold blood cardioplegia and LIMA continuous cold blood perfusion. Distal anastomosis was performed under selective cerebral perfusion and during deep hypothermic circulatory arrest. Postoperative course was satisfactory and the patient was discharged without complications.
- Published
- 2003
137. Endotoxinemia and benzodiazepine-like substances in compensated cirrhotic patients: a randomized study comparing the effect of rifaximine alone and in association with a symbiotic preparation
- Author
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C H Min, Y Naito, A Helmy, P Hotten, Francesco Marotta, M Yoshioka, H Fuji, and J Lighthouse
- Subjects
Benzodiazepine ,medicine.medical_specialty ,Lactobacillus helveticus ,Cirrhosis ,Hepatology ,biology ,business.industry ,medicine.drug_class ,biology.organism_classification ,medicine.disease ,Gastroenterology ,Surgery ,law.invention ,Regimen ,Infectious Diseases ,Lactobacillus acidophilus ,Randomized controlled trial ,Rifaximine ,law ,Internal medicine ,Medicine ,business ,Hepatic encephalopathy - Abstract
Aim: The aim of the present investigation was to test study benzodiazepines (BZDs) profile in patients with viral cirrhosis under different combinations of rifaximine and of a novel symbiotic. Methods: Our study groups consisted of 30 patients with a confirmed diagnosis of HCV-related Child B liver cirrhosis. Patients were randomly allocated into three groups: rifaximine 400mg t.i.d. for 2 weeks; (B) SCM-III (Lactobacillus acidophilus, Lactobacillus helveticus and Bifidobacteria in a ion- and vitamin-enriched medium, Named srl, Italy) 10ml t.i.d. for 2 weeks; (C) rifaximine 400mg t.i.d. for 1 week followed by SCM-III 10ml t.i.d. for 5 weeks. At weekly interval, blood samples were withdrawn to test BZD-like substances, ammonia and endotoxin. Results: Rifaximine treatment brought about a significant early drop of BZDs ( [Formula: see text] versus pre-treatment and versus control) till fourth week of observation when a gradual increase took place with return to pre-treatment values at the sixth week. Symbiotic treatment was comparably effective while given to patients but significantly elevated BZDs level were noted starting from the third week. Similar phenomena were noted for endotoxin and ammonia although symbiotic seemed more effective against endotoxin and rifaximine against ammonia increase. However, the sequential treatment rifaximine-symbiotic brought about a sustained normalization of BZDs, ammonia and endotoxin throughout the 6-week study. Conclusion: The present pilot study suggests that a rifaximine-symbiotic regimen could be an effective tool in compensated liver cirrhosis to limit some triggering factors of hepatic encephalopathy while being amenable to long-term use and devoid of significant side effects.
- Published
- 2003
138. Incremental prediction of the side-chain conformation of proteins by a genetic algorithm
- Author
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Y. Naito and H. Iijima
- Subjects
Quantitative Biology::Biomolecules ,symbols.namesake ,Atom ,Genetic algorithm ,symbols ,Side chain ,A protein ,Thermodynamics ,Van der Waals radius ,van der Waals force ,Cellular biophysics - Abstract
The paper reports an approach for predicting the side-chain conformation of a protein using a genetic algorithm: an incremental GA. In the GA, the chromosome consists of an array of side-chain torsion angles(/spl chi/), and the fitness is only calculated from overlap among each atom's van der Waals radius under the assumption of fixed main-chain atoms. The GA was incrementally used in the simulation. No van der Waals contact was observed in the predicted structures. This process automatically conforms most of the large side-chains in the hydrophobic core exactly the same in comparison with physical methods such as X-ray crystallography. This approach shows other solutions than the native tertiary conformation in some small side-chain positions. The observed conformation shows that the incremental GA has abilities in predicting side-chain conformation. >
- Published
- 2002
139. A 256 Mb DRAM with 100 MHz serial I/O ports for storage of moving pictures
- Author
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H. Kotani, H. Akamatsu, Y. Naito, T. Fujii, T. Iwata, T. Tsuji, H. Asaka, Y. Itoh, N. Shimizu, J. Hirase, Y. Shibata, K. Yamashita, T. Hori, and T. Fujita
- Published
- 2002
140. Design and drawing support system for photovoltaic array structure
- Author
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T. Hirasawa, Y. Naito, N. Asayama, K. Iida, and S. Yamaguchi
- Subjects
Operator (computer programming) ,Computer science ,Limit (music) ,Photovoltaic system ,Structure (category theory) ,Array data structure ,Computer Aided Design ,Electronic design automation ,Topology ,computer.software_genre ,computer ,Finite element method - Abstract
This paper describes a design and drawing support system for a photovoltaic (PV) array structure. The operator inputs data (e.g. structure type, tilt angle, load conditions, etc.) into the system, which computes stress on each element of structure and outputs the calculated results. If the results are within the tolerance limit, a skeleton drawing of the structure is produced. The weight list of materials is also outputted on paper. When the results are beyond the limit, the data is modified by the operator and re-computed until the satisfactory results are shown.
- Published
- 2002
141. The Seebeck effect in highly conductive mu c-Ge:H films and its application to sensors
- Author
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S. Kodato, K. Kuroda, and Y. Naito
- Subjects
Responsivity ,Materials science ,Microcrystalline ,Semiconductor ,business.industry ,Seebeck coefficient ,Thermoelectric effect ,Electronic engineering ,Optoelectronics ,Optical power ,Chemical vapor deposition ,business ,Thermopile - Abstract
By combining a plasma CVD (chemical vapor deposition) system with a real-time annealing monitoring system, a highly conductive mu c-Ge:H film has been successfully fabricated on a glass substrate. This film generates a large amount of thermoelectric power (maximum -420 mu V/K). Using this microcrystalline Ge:H film thermopile, an optical power sensor and thermal vacuum gauge have been developed. In particular, when this mu c-Ge:H thermopile was used together with a low-reflectance Ni-P optical absorber, a high-accuracy, quick-response laser beam power sensor with the following characteristics was realized: responsivity, 0.3 mV/mW; response time, 1.8 s; wavelength range, 0.4 to 1.8 mu m; diameter of active area, 6 mm phi ; and resistance, 600 Omega to 1 k Omega . >
- Published
- 2002
142. Density protuberances created by moderately high power microwave pulses propagating in corrugated plasma waveguides
- Author
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H. Onose, N. Hisyam, Kazuo Minami, S. Kobayashi, Y. Naito, A. Sugawara, Koji Tanaka, and V.L. Granatstein
- Subjects
Materials science ,business.industry ,Plasma ,Microwave propagation ,law.invention ,Power (physics) ,Optics ,law ,Wave structure ,Radio frequency ,business ,Waveguide ,Microwave ,Plasma density - Abstract
Summary form only given. Protuberances (local maxima) in plasma density in the axial direction created by microwave pulses externally injected into a plasma-filled corrugated wall waveguide (slow wave structure, SWS) are observed experimentally. This is observed for moderately high microwave powers above a threshold value repeatedly under various conditions.
- Published
- 2002
143. H.263 mobile video codec based on a low power consumption digital signal processor
- Author
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Y. Naito and I. Kuroda
- Subjects
Digital signal processor ,Image quality ,Computer science ,business.industry ,Real-time computing ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Data_CODINGANDINFORMATIONTHEORY ,Enhanced Variable Rate Codec ,Frame rate ,Adaptive Multi-Rate audio codec ,Filter (video) ,Motion estimation ,Codec ,business ,Digital signal processing ,Data compression - Abstract
This paper describes an H.263 video codec implementation based on a low power consumption general purpose DSP. Fast algorithms, such as a fast motion estimation algorithm and a low complexity noise reduction filter, are proposed to implement the video codec on a single DSP chip maintaining sufficient picture quality. By using a 50 MIPS, 100 mW DSP, the developed codec encodes and decodes 7.5 QCIF frames per second, which is sufficient performance for low bit-rate video compression, typically below 64 kbps.
- Published
- 2002
144. Modeling of gaseous fire suppression system by means of bond graph
- Author
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T. Akino, Y. Naito, Nobuyoshi Kawabata, Takuji Ishikawa, K. Tanaka, and Y. Matsumoto
- Subjects
Engineering ,business.industry ,Gaseous fire suppression ,System safety ,Duct (flow) ,Structural engineering ,Mechanics ,Gas cylinder ,business ,Bond graph - Abstract
The gaseous fire suppression system (GFSS) has excellent features such as the fact that equipment can be restored early after fire accidents. So these systems have rapidly spread recently. The purpose of the present study is to develop a simulator of GFSSs by using the bond graph that is often used for dynamic characteristic analysis of hydraulic and pneumatic systems. In this study, a gas cylinder, pipe, a protection room and a pressure relief duct are expressed by the bond graph and the injection of the extinction gas into the protection room is simulated. The result of the simulation is compared with that of the experiment, which shows good agreement. It is also clarified that the maximum indoor pressure greatly exceeds the pressure obtained from the conventional design method.
- Published
- 2002
145. [Extracardiac fontan procedure in adult: report of a case]
- Author
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Y, Naito, Y, Imai, K, Seo, M, Aoki, T, Shin-oka, K, Hiramatsu, J, Ota, J, Aiba, and K, Monma
- Subjects
Adult ,Pulmonary Valve Stenosis ,Atrial Flutter ,Pulmonary Veins ,Heart Septal Defects ,Humans ,Female ,Cardiac Surgical Procedures ,Fontan Procedure ,Double Outlet Right Ventricle - Abstract
We report a case of a 28-year-old female who underwent an extracardiac Fontan procedure. The subject was diagnosed as an atrioventricular septal defect (Rastelli classification: type C), a double outlet right ventricle, pulmonary artery stenosis, a hypoplasty of left ventricle, total anomalous venous return (Darling: Ib + IIb), and atrial flutter. She underwent a Blalock shunt and an aorto-pulmonary shunt at the ages of 3 and 9 years, respectively. Under a total CPB, an extracardiac total cavo-pulmonary connection (TCPC), using a 26 mm Hemashield graft, was completed. The postoperative course was uneventful. The complicated atrial anatomy and atrial arrhythmia indicated TCPC in this adult patient.
- Published
- 2002
146. [First successful clinical application of tissue engineered blood vessel]
- Author
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N, Hibino, Y, Imai, T, Shin-oka, M, Aoki, M, Watanabe, Y, Kosaka, G, Matsumura, T, Konuma, S, Toyama, A, Murata, Y, Naito, and T, Miyake
- Subjects
Blood Vessel Prosthesis Implantation ,Tissue Engineering ,Child, Preschool ,Heart Ventricles ,Humans ,Female ,Pulmonary Artery ,Plastic Surgery Procedures ,Fontan Procedure ,Double Outlet Right Ventricle - Abstract
With this tissue engineering (TE) technique, the peripheral pulmonary artery was successfully reconstructed, using the patient's own venous cells in a 4-year-old girl, 2 years after Fontan procedure. A 4-year-old girl was given a diagnosis of single right ventricle, double-outlet right ventricle and pulmonary atresia. She underwent left modified Blalock-Taussig shunt at a month old, pulmonary artery angioplasty at a year and 3 months old, and bidirectional cavopulmonary shunt at 2 years and a month old. She underwent again pulmonary artery angioplasty and Fontan operation at 3 years and 3 months. An angiographical examination 7 months after the operation revealed total occlusion of the right intermediate pulmonary artery. TE technique using autologous cells was indicated. The application of this procedure was approved by the ethical committee in Tokyo Women's Medical University. The patient's parents were thoroughly informed and signed a consent form. Approximately 2 cm of the peripheral vein was explanted under sterile conditions. The tissue was minced, placed in tissue culture dishes and cultured at 37 degrees C, 100% humidity and a 5% CO2 atmosphere for almost a month. The number of cells substantially increased to reach 12 millions for almost a month. The culture medium was changed every 3 days. The polymer tube that served as a scaffold for cells was composed of the copolymer of PCL-PLA (50:50) with reinforcement by woven PGA. The polymer conduit, 10 mm in diameter, 20 mm in length and 1 mm in thickness, was designated to biodegradate within 8 weeks. The number of seeded cells was approximately a million/cm2. The graft transplantation was performed 10 days after seeding cells. The occlusive right intermediate pulmonary artery was reconstructed with the TE vessel graft under extracorporeal circulation with a pump-oxygenator. The patient followed a satisfactory postoperative course. The postoperative angiography demonstrated that the graft was not constricted and dilated but that it preserved good patency. Long-term follow-up are necessary. We plan to continue to use the TE technique using autologous cells in the low pressure system like venous or pulmonary circulation. Because our results even in early experimental phase were valuable and promising, we believe that the TE approach may play an important role in the near future as an another alternative, together with transplantation and artificial organ, especially in the field of cardiovascular surgery that mostly needs replants.
- Published
- 2002
147. Role of elastase and active oxygen species in gastric mucosal injury induced by aspirin administration in Helicobacter pylori-infected Mongolian gerbils
- Author
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N, Yoshida, N, Sugimoto, J, Ochiai, Y, Nakamura, H, Ichikawa, Y, Naito, and T, Yoshikawa
- Subjects
Male ,Sulfonamides ,Aspirin ,Helicobacter pylori ,Superoxide Dismutase ,Glycine ,Free Radical Scavengers ,Catalase ,Thiobarbituric Acid Reactive Substances ,Helicobacter Infections ,Gastric Mucosa ,Animals ,Stomach Ulcer ,Gerbillinae ,Leukocyte Elastase ,Reactive Oxygen Species ,Peroxidase - Abstract
H. pylori infection potentiates aspirin-induced gastric mucosal injury by mechanisms that include accumulation of activated neutrophils.To determine the role of elastase and active oxygen species (AOS) produced by activated neutrophils in the gastric mucosal injury induced by administration of acidified aspirin to H. pylori-infected Mongolian gerbils.H. pylori ATCC43504 culture broth was administered by oral gavage to male Mongolian gerbils at 7 weeks of age. After 4 weeks, acidified aspirin (400 mg/kg) was administered orally, and 3 h later, the total area of gastric erosions, myeloperoxidase (MPO) activity (an index of neutrophil accumulation), thiobarbituric acid-reactive substances (TBARS, an index of lipid peroxidation), and KC/GRO (a chemo-attractive cytokine in rodents) were measured in gastric mucosa. To determine the role of elastase or AOS derived from neutrophils in these circumstances, ONO-5046 (an elastase inhibitor), a combination of superoxide dismutase (SOD) and catalase (scavengers of AOS), and polaprezinc (an anti-ulcer agent with anti-inflammatory effects) were administered before aspirin.ONO-5046 inhibited the increase in gastric erosions and mucosal TBARS induced by administration of aspirin to H. pylori-infected gerbils, but not the increases in MPO activity or KC/GRO contents. A combination of SOD and catalase or polaprezinc significantly reduced gastric erosions, TBARS concentrations, MPO activity and KC/GRO concentration.These results suggest that neutrophil-derived-elastase and -oxidants play an important role in the gastric mucosal injury induced by administration of aspirin to H. pylori-infected gerbils.
- Published
- 2002
148. Ubiquitin-proteasome inhibitor enhances tumour necrosis factor-alpha-induced apoptosis in rat gastric epithelial cells
- Author
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Y, Naito, O, Handa, T, Takagi, T, Ishikawa, E, Imamoto, S, Nakagawa, T, Yamaguchi, N, Yoshida, H, Matsui, and T, Yoshikawa
- Subjects
Proteasome Endopeptidase Complex ,Membrane Glycoproteins ,Cell Survival ,Tumor Necrosis Factor-alpha ,Ubiquitin ,Blotting, Western ,Calcium-Binding Proteins ,NF-kappa B ,Apoptosis ,Epithelial Cells ,Nerve Tissue Proteins ,Flow Cytometry ,Cell Line ,Rats ,DNA-Binding Proteins ,Cysteine Endopeptidases ,Synaptotagmins ,NF-KappaB Inhibitor alpha ,Gastric Mucosa ,Multienzyme Complexes ,Synaptotagmin I ,Animals ,I-kappa B Proteins ,Microscopy, Phase-Contrast ,Oligopeptides - Abstract
Tumour necrosis factor (TNF-alpha) is a candidate factor for involvement in inflammation-mediated gastric mucosal injury. However, the effect of this cytokine on gastric epithelial cells has been poorly investigated. In the present study, we examined whether gastric epithelial cells are resistant to TNF-alpha-induced apoptosis, and whether this resistance is related to ubiquitin-proteasome-associated nuclear factor-kappaB (NF-kappaB) activation.The rat gastric mucosal cell line RGM-1 was grown in DMEM/F12 medium supplemented with 10% FCS. Confluent monolayers of cells were pretreated or not for 60 min with PSI, a peptide aldehyde known to specifically inhibit the chymotrypsin-like activity of 26S proteasome. Cells were subsequently stimulated with recombinant rat TNF-alpha and their viability was determined by WST-1 assay. Apoptosis was confirmed by fluorescence microscopy after staining with Hoechst 33342 and propidium iodide, and DNA fragmentation was determined by flow cytometry using an APO-BRDU kit. IkappaB-alpha and the p65 binding subunit of NF-kappaB were detected by Western blots.Twenty-four-hour incubation with TNF-alpha alone or PSI alone did not affect the cell viability of RGM-1 cells. Pretreatment with PSI significantly enhanced the level of apoptosis induced by TNF-alpha. In RGM-1 cells treated with TNF-alpha, cytoplasmic IkappaB-alpha decreased and p65 in nuclear extracts increased markedly 30 min after cytokine stimulation. Pretreatment with PSI at 12.5 micromol/L blocked these TNF-alpha-induced changes.PSI enhances TNF-alpha-induced apoptosis through inhibition of NF-kappaB activation in RGM-1 cells.
- Published
- 2002
149. Buoyancy and maximal diving depth in penguins: do they control inhaling air volume?
- Author
-
Katsufumi, Sato, Y, Naito, A, Kato, Y, Niizuma, Y, Watanuki, J B, Charrassin, C-A, Bost, Y, Handrich, and Y, Le Maho
- Subjects
Birds ,Species Specificity ,Air ,Diving ,Respiratory Mechanics ,Animals ,Lung Volume Measurements ,Biomechanical Phenomena - Abstract
Using a newly developed data logger to measure acceleration, we demonstrate that free-ranging king and Adélie penguins only beat their flippers substantially during the first part of descent or when they were presumed to be chasing prey at the bottom of dives. Flipper beating stopped during the latter part of ascent: at 29+/-9 % (mean +/- S.D.) of dive depth (mean dive depth=136.8+/-145.1 m, N=425 dives) in king penguins, and at 52+/-20 % of dive depth (mean dive depth=72.9+/-70.5 m, N=664 dives) in Adélie penguins. Propulsive swim speeds of both species were approximately 2 m s(-1) during dives; however, a marked increase in speed, up to approximately 2.9 m s(-1), sometimes occurred in king penguins during the passive ascending periods. During the prolonged ascending, oblique ascent angle and slowdown near the surface may represent one way to avoid the potential risk of decompression sickness. Biomechanical calculations for data from free-ranging king and Adélie penguins indicate that the air volume of the birds (respiratory system and plumage) can provide enough buoyancy for the passive ascent. When comparing the passive ascents for shallow and deep dives, there is a positive correlation between air volume and the depth of the dive. This suggests that penguins regulate their air volume to optimize the costs and benefits of buoyancy.
- Published
- 2002
150. Acquisition of multidrug resistance in recurrent breast cancer demonstrated by the histoculture drug response assay
- Author
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H, Tanino, S, Oura, R M, Hoffman, T, Kubota, T, Furukawa, J, Arimoto, T, Yoshimasu, I, Hirai, T, Bessho, T, Suzuma, T, Sakurai, and Y, Naito
- Subjects
Adult ,Aged, 80 and over ,Male ,Breast Neoplasms ,Middle Aged ,Drug Resistance, Multiple ,Breast Neoplasms, Male ,Methotrexate ,Doxorubicin ,Drug Resistance, Neoplasm ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Female ,Fluorouracil ,Drug Screening Assays, Antitumor ,Neoplasm Recurrence, Local ,Cyclophosphamide ,Aged ,Epirubicin ,Neoplasm Staging - Abstract
Recurrent breast cancer has a very poor response rate to chemotherapy. To understand the degree of acquisition of multidrug resistance in recurrent disease, 24 recurrent breast tumors and 127 primary tumors were evaluated and compared for chemosensitivity in the histoculture drug response assay (HDRA). The evaluation rate was 98.8%. The HDRA utilizes 3-dimensional culture of human tumors on collagen-gel rafts. Doxorubicin (DXR), 5-fluorouracil (5-FU) and mitomycin C (MMC) were tested as standard agents and cisplatin (CDDP) as a candidate agent on surgical specimen of breast cancer in the HDRA. In vitro drug exposure in the HDRA was for 7 days. At the end of the assay, tumor response was assessed by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The mean inhibition rates of primary tumors vs. recurrent tumors were 57.9% and 38.6% for DXR (p0.0005); 59.9% and 42.8% for MMC (p0.01); 49.0% and 33.4% for 5-FU (p0.01); and 34.5% and 16.0% for CDDP (p0.005), respectively. The recurrent cases were pretreated clinically with CAF (cyclophosphamide, DXR and 5-FU), CEF (cyclophosphamide, epirubicin and 5-FU) or CMF (cyclophosphamide, methotrexate and 5-FU). In the CAF and CEF group, the HDRA sensitivity to CDDP was significantly lower in recurrent disease (p0.005) than that of primary breast cancer suggesting that one agent can induce resistance to another. This is further suggested by the fact that 64.7% of the recurrent cases were resistant to all 4 agents tested as opposed to 27% of the primary cases and that only 5.9% of the recurrent cases were sensitive to three or more agents as opposed to 18% of the primary cases. The correlation of the HDRA results to clinical outcome in the study was 80.0% with 15 cases evaluated consisting of 5 true positives, 3 false positives, 7 true negatives and no false negatives. Thus, the HDRA gives useful clinical information, in particular for the specific individualized treatment design necessary to overcome the multidrug resistance problem of recurrent breast cancer.
- Published
- 2002
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