147 results on '"Garza JA"'
Search Results
102. Lower than expected mortality with dexamethasone in toxic epidermal necrolysis: 30-year experience of a Northern Mexico reference hospital.
- Author
-
Gómez-Flores M, Villarreal-Villarreal CD, Welsh O, Cárdenas-de la Garza JA, Sánchez-Meza E, Áncer-Arellano J, Martinez-Moreno A, Ocampo-Candiani J, and Herz-Ruelas ME
- Published
- 2020
- Full Text
- View/download PDF
103. Colorectal Cancer and Polyps in Diverticulosis Patients: A 10-Year Retrospective Study in 13680 Patients.
- Author
-
Abu Baker F, Z'cruz De La Garza JA, Mari A, Zeina AR, Bishara A, Gal O, and Kopelman Y
- Abstract
Introduction: Shared by certain epidemiological and etiological characteristics, diverticulosis and colorectal cancer (CRC) as well as colonic polyps has long been linked. This association was studied in several heterogeneous studies but has reported inconsistent results. Clarifying the association is clinically relevant for endoscopist awareness and potential modification of screening and surveillance intervals for diverticulosis patients., Methods: In this retrospective single-center study, patients diagnosed with diverticulosis on colonoscopy over a 10-year period were included. Each diverticulosis patient was matched with 1 control by age, gender, setting (inpatient/outpatient), and procedure's indication. CRC and polyp detection rates were recorded and compared between the groups before and after adjustment for bowel preparation quality and exam completion. CRC location was recorded and compared between groups., Results: A cohort of 13680 patients (6840 patients with diverticulosis and 6840 matched controls) was included. Diverticulosis was located mainly to the sigmoid and left colon (94.4%). The CRC diagnosis rate was lower in the diverticulosis group (2% vs. 4.5%, odds ratio = 0.472, P < 0.001, and 95%CI = 0.382-0.584). Moreover, location of CRC was unrelated to diverticulosis location, as more CRCs in the diverticulosis group were located proximal to the splenic flexure as compared to the control group (42.5% vs 29.5%, respectively; P = 0.007). Diverticulosis, however, was associated with an increased polyp detection rate compared to controls (30.5% vs. 25.5%; odds ratio = 1.2, P < 0.001, and 95%CI = 1.11-1.299)., Conclusion: We demonstrated that diverticulosis was not associated with an increased risk for CRC. A possible increased polyp detection rate, however, warrants further evaluation in large prospective studies., Competing Interests: The authors certify that they have no affiliations with or involvement in any organization or entity with any financial or nonfinancial interest in the subject matter or materials discussed in this manuscript. The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (Copyright © 2019 Fadi Abu Baker et al.)
- Published
- 2019
- Full Text
- View/download PDF
104. How Does Comprehensive Care Impact Life of Pediatric Patients With Hemophilia? Results From a Center in a Developing Country.
- Author
-
Villarreal-Martínez L, Meléndez-Aguirre A, Cantú-Moreno M, Garay-Mendoza D, Garza JA, Ibarra-Ramírez M, Quiroga-Treviño M, Pope-Salazar SE, and Velasco-Ruiz I
- Subjects
- Adolescent, Child, Child, Preschool, Female, Health Status, Hemophilia A diagnosis, Humans, Male, Mexico, Treatment Outcome, Hemophilia A therapy, Quality of Life, Surveys and Questionnaires
- Abstract
Background: Quality of life (QoL) has been included as a marker of treatment effectiveness in pediatric patients with chronic diseases. We believe that frequent multidisciplinary interventions and patient education could lead to an improvement in QoL., Aims: Determine the QoL and economic impact of monthly interventions in multidisciplinary treatment., Materials and Methods: The Haemo-QoL questionnaire was applied to patients who attended the hemophilia center of the University Hospital "Dr. José Eleuterio González," Monterrey, Mexico, at the time of enrollment and 1 year later., Results: Male patients between 4 and 16 years diagnosed with hemophilia were included. The score results presented are based on Haemo-QoL versions that classify patients by their age group: group 1 (4 to 7 y) and group 2 (8 to 12 y). Statistical significant improvement was observed in the overall score (sociodemographic, psychosocial, etc.) after 1 year of follow-up in both groups (P<0.05)., Conclusions: Impact on the QoL of patients receiving this approach was favorable. Improvement was observed regardless of severity and in those who were already in prophylaxis, suggesting that this type of approach could be causing the improvement. Results support the application of multidisciplinary treatment as the gold standard, and it should be considered in all centers including those with limited resources.
- Published
- 2019
- Full Text
- View/download PDF
105. Electrodissection for nevus lipomatosus cutaneous superficialis removal.
- Author
-
Ancer-Arellano J, Villarreal-Villarreal CD, Cardenas-de la Garza JA, Cuellar-Barboza A, Vazquez-Martínez O, and Ocampo-Candiani J
- Subjects
- Dermatologic Surgical Procedures methods, Humans, Lipomatosis complications, Male, Nevus complications, Skin Neoplasms complications, Treatment Outcome, Young Adult, Electrosurgery, Lipomatosis surgery, Nevus surgery, Skin Neoplasms surgery
- Published
- 2019
- Full Text
- View/download PDF
106. Regarding the article 'Prevalence of eosinophilic esophagitis: A multicenter study on a pediatric population evaluated at thirty-six Latin American gastroenterology centers' by Pierre-Álvarez et al.
- Author
-
Madrazo-de la Garza JA
- Subjects
- Child, Humans, Latin America, Prevalence, Eosinophilic Esophagitis, Gastroenterology
- Published
- 2019
- Full Text
- View/download PDF
107. Classic dermatological tools: Foreign body removal with punch biopsy.
- Author
-
Cardenas-de la Garza JA, Cuellar-Barboza A, Ancer-Arellano J, Ramos-Cavazos CJ, Vazquez-Martinez OT, and Ocampo-Candiani J
- Subjects
- Animals, Biopsy instrumentation, Granuloma, Foreign-Body etiology, Humans, Sea Urchins, Treatment Outcome, Granuloma, Foreign-Body therapy, Skin
- Published
- 2019
- Full Text
- View/download PDF
108. Acral bullous lichen sclerosus intolerant to UVA-1 successfully treated with narrowband ultraviolet B phototherapy.
- Author
-
Herz-Ruelas ME, Barboza-Quintana O, Cuéllar-Barboza A, Cárdenas-de la Garza JA, and Gómez-Flores M
- Subjects
- Female, Humans, Middle Aged, Lichen Sclerosus et Atrophicus pathology, Lichen Sclerosus et Atrophicus radiotherapy, Pain pathology, Pain radiotherapy, Quality of Life, Ultraviolet Rays adverse effects, Ultraviolet Therapy
- Abstract
Lichen sclerosus (LS) is an uncommon, chronic, lymphocyte-mediated, inflammatory dermatosis characterized by ivory-white patches with scar-like atrophy. Extragenital bullous lichen sclerosus may rarely affect palms and soles, causing severe pain and substantially impairing quality of life. We present the first case of acral bullous lichen sclerosus intolerant to UVA-1 phototherapy successfully treated with low doses of narrowband ultraviolet B phototherapy., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2019
- Full Text
- View/download PDF
109. Red Scrotum Syndrome Treatment with Pregabalin: A Case Series.
- Author
-
Cardenas-de la Garza JA, Villarreal-Villarreal CD, Cuellar-Barboza A, Cardenas-Gonzalez RE, Gonzalez-Benavides N, Cruz-Gomez LG, Ocampo-Candiani J, and Welsh O
- Abstract
Red scrotum syndrome (RSS) (also known as male genital dysesthesia) is a rarely recognized entity characterized by scrotal erythema accompanied by a burning sensation, pain, hyperesthesia/dysesthesia, increased temperature and pruritus. Although its physiopathology is unknown, it has increasingly been associated with chronic topical steroid use in the male genital area. Treatment is challenging and no standardized treatment is currently available. Because current treatment relies on case reports and small case series, the need for more information about drug efficacy in RSS is warranted. The aim of this study is to describe the therapeutic response to pregabalin in patients from an outpatient dermatologic clinic in a tertiary-care hospital diagnosed with RSS. Five patients with a confirmed diagnosis of RSS were included. Ages ranged from 28 to 63 years. All patients had chronic steroid use in the genital area, mostly in the form of combined formulations of corticosteroids, antifungals, and antibiotics. Four patients were prescribed pregabalin monotherapy, 150 mg once daily at night. One patient was prescribed pregabalin and doxycycline. Two patients had complete remission after one month of therapy, one at two months and two at three months. None experienced recurrence at an average of 9.4 months' follow-up. One patient experienced morning drowsiness that did not require suspending treatment. Pregabalin is a well-tolerated and effective treatment for RSS., Competing Interests: CONFLICTS OF INTEREST: The authors have nothing to disclose., (Copyright © 2019 The Korean Dermatological Association and The Korean Society for Investigative Dermatology.)
- Published
- 2019
- Full Text
- View/download PDF
110. Giant Lumbar Polypoid Tumor with Bullae on its Surface.
- Author
-
Cuellar-Barboza A, Cardenas-de la Garza JA, Martinez-Moreno A, Cardenas-Gonzalez R, Barboza-Quintana O, and Ocampo-Candiani J
- Subjects
- Adult, Diagnosis, Differential, Humans, Male, Blister diagnosis, Lumbosacral Region, Skin Neoplasms diagnosis
- Abstract
Dear Editor, Acrochordons are common benign neoplasms found in everyday practice. We present a rare case of a giant and single acrochordon on the lumbar region with signs of ischemia. The correlation of clinical, dermatoscopic, and histopathological analyses may help the clinician differentiate this entity from other benign and malignant dermatoses. A 33-year-old man presented to the outpatient clinic complaining of a lumbar mass that had been growing for two years. The lesion was asymptomatic except for recent discomfort when lying in the supine decubitus position. On examination there was a single, erythematous, non-tender pedunculated lesion, 5.1 cm × 4 cm × 3.2 cm in size, with flaccid bullae on its surface and firm consistency (Figure 1, a-b). Dermoscopy showed regularly arranged dotted vessels in a violaceous background and bullae filled with serous fluid (Figure 1, c-d). His personal and family medical history were unremarkable. Shave excision was performed and the sample was sent for histopathologic analysis. Histopathology revealed a normal epidermis surrounding a core of loose collagen fibers. Papillary dermis showed a mixed inflammatory cell infiltrate, congested capillaries, and extravasated erythrocytes. Scattered clusters of adipocytes were found in the deep dermis (Figure 2). The diagnosis of a giant ischemic acrochordon was established. Acrochordons, also commonly known as skin tags, are benign pedunculated neoplasms frequently found on the neck, axillae, or groin. They represent fibroepithelial polyps that originate from ectoderm and mesoderm tissue (1). They can be pigmented or skin colored and usually range between 2 to 5 mm in size. Skin tags can affect nearly 46% of the general population (2). Giant and solitary acrochordons are an uncommon clinical variant (2). Differential diagnosis of this pathology may be challenging in the particular clinical context of this patient. Benign neoplasms such as dermatofibroma, neurofibroma, and epidermal inclusion cysts can be clinically similar to giant skin tags (1,2). Histopathological analyses of acrochordons usually show papillomatosis, irregular acanthosis, loose collagen fibers, and dilated capillaries (2). A fibrolipomatous variant of acrochordon may reveal adipose tissue lobules between septa of collagen on pathological examination (1). Local ischemia due to twisting of the stalk may induce the formation of intradermal or subepidermal blisters in these tumors. Histopathologic characteristics found in ischemic acrochordons are neutrophil infiltration, congested capillaries, and extravasated erythrocytes (3). Fibroepithelioma of Pinkus (FeP) is a rare variant of basal cell carcinoma that may clinically mimic benign polypoid tumors and has a certain predilection for the lumbosacral area. Dermoscopic characteristics of fibroepithelioma of Pinkus that may aid the clinician in this diagnosis are fine arborizing vessels, either alone or associated with dotted vessels, and white streaks (4). Cosmetically adequate treatment of a giant acrochordon can be performed by shave excision and electrodesiccation of its pedicle. Choudhary et al. reported good results with this technique on a giant acrochordon on the thigh (5).
- Published
- 2019
111. Dermoscopic findings of umbilical granuloma.
- Author
-
Ancer-Arellano J, Argenziano G, Villarreal-Martinez A, Cardenas-de la Garza JA, Villarreal-Villarreal CD, and Ocampo-Candiani J
- Subjects
- Anti-Infective Agents, Local therapeutic use, Female, Granuloma drug therapy, Humans, Infant, Silver Nitrate therapeutic use, Skin Diseases drug therapy, Dermoscopy, Granuloma pathology, Skin Diseases pathology, Umbilicus
- Abstract
Dermoscopic findings in umbilical granuloma are vascular polymorphism comprised of linear irregular and arborizing vessels with structureless areas distributed over a milky-red background. The increase of angiogenesis and neovascularization is represented by the linear irregular and arborizing vessels. Structureless areas over an irregularly milky-red background are originated by the proliferation of vascular endothelial cells and fibroblasts, with capillary and granulation tissue formation., (© 2019 Wiley Periodicals, Inc.)
- Published
- 2019
- Full Text
- View/download PDF
112. Disseminated Trichosporon asahii infection in a patient with acute myeloid leukemia.
- Author
-
Cardenas-de la Garza JA, Ancer-Arellano J, Cuellar-Barboza A, Saenz-Ibarra B, Ocampo-Garza SS, Barboza-Quintana O, Gallardo-Rocha A, Vera-Cabrera L, Ocampo-Candiani J, and Welsh O
- Subjects
- Amphotericin B therapeutic use, Antifungal Agents therapeutic use, Biopsy, Fatal Outcome, Female, Fungemia drug therapy, Fungemia microbiology, Fungemia prevention & control, Humans, Immunocompromised Host, Itraconazole therapeutic use, Middle Aged, Skin microbiology, Skin pathology, Treatment Failure, Trichosporonosis drug therapy, Trichosporonosis microbiology, Trichosporonosis prevention & control, Antibiotic Prophylaxis methods, Fungemia diagnosis, Leukemia, Myeloid, Acute immunology, Trichosporon isolation & purification, Trichosporonosis diagnosis
- Published
- 2019
- Full Text
- View/download PDF
113. Clostridium strain selection for co-culture with Bacillus subtilis for butanol production from agave hydrolysates.
- Author
-
Oliva-Rodríguez AG, Quintero J, Medina-Morales MA, Morales-Martínez TK, Rodríguez-De la Garza JA, Moreno-Dávila M, Aroca G, and Rios González LJ
- Subjects
- Anaerobiosis, Coculture Techniques, Fermentation, Agave metabolism, Bacillus subtilis metabolism, Butanols metabolism, Clostridium acetobutylicum metabolism
- Abstract
In this work, three Clostridium strains were tested for butanol production from Agave lechuguilla hydrolysates to select one for co-culturing. The agave hydrolysates medium was supplemented with nutrients and reducing agents to promote anaerobiosis. Clostridium acetobutylicum ATCC 824 had the highest butanol production (6.04 g/L) and was selected for further analyses. In the co-culture process, Bacillus subtilis CDBB 555 was used to deplete oxygen and achieve anaerobic conditions required for butanol production. The co-culture was prepared with C. acetobutylicum and B. subtilis without anaerobic pretreatment. Butanol production in co-culture from agave hydrolysates was compared with experiments using synthetic medium with glucose and a pure culture of C. acetobutylicum. The maximum butanol concentration obtained was 8.28 g/L in the co-cultured hydrolysate medium. Results obtained in the present work demonstrated that agave hydrolysates have the potential for butanol production using a co-culture of B. subtilis and C. acetobutylicum without anaerobic pretreatment., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
114. Clinical spectrum of Lyme disease.
- Author
-
Cardenas-de la Garza JA, De la Cruz-Valadez E, Ocampo-Candiani J, and Welsh O
- Subjects
- Animals, Anti-Bacterial Agents therapeutic use, Borrelia burgdorferi Group classification, Borrelia burgdorferi Group drug effects, Clinical Laboratory Techniques, Humans, Ixodes classification, Ixodes microbiology, Prognosis, Treatment Outcome, Borrelia burgdorferi Group physiology, Lyme Disease diagnosis, Lyme Disease drug therapy, Lyme Disease pathology, Lyme Disease physiopathology, Skin Diseases, Bacterial diagnosis, Skin Diseases, Bacterial drug therapy, Skin Diseases, Bacterial pathology, Skin Diseases, Bacterial physiopathology
- Abstract
Lyme disease (borreliosis) is one of the most common vector-borne diseases worldwide. Its incidence and geographic expansion has been steadily increasing in the last decades. Lyme disease is caused by Borrelia burgdorferi sensu lato, a heterogeneous group of which three genospecies have been systematically associated to Lyme disease: B. burgdorferi sensu stricto Borrelia afzelii and Borrelia garinii. Geographical distribution and clinical manifestations vary according to the species involved. Lyme disease clinical manifestations may be divided into three stages. Early localized stage is characterized by erythema migrans in the tick bite site. Early disseminated stage may present multiple erythema migrans lesions, borrelial lymphocytoma, lyme neuroborreliosis, carditis, or arthritis. The late disseminated stage manifests with acordermatitis chronica atrophicans, lyme arthritis, and neurological symptoms. Diagnosis is challenging due to the varied clinical manifestations it may present and usually involves a two-step serological approach. In the current review, we present a thorough revision of the clinical manifestations Lyme disease may present. Additionally, history, microbiology, diagnosis, post-treatment Lyme disease syndrome, treatment, and prognosis are discussed.
- Published
- 2019
- Full Text
- View/download PDF
115. Skeletal Muscle Diffuse Large B-Cell Lymphoma in the Gluteal Region
- Author
-
Gonzalez-Benavides N, Cardenas-de la Garza JA, Rodriguez-Vivian C, Ocampo-Candiani J, and Welsh O
- Subjects
- Humans, Male, Middle Aged, Muscle Neoplasms pathology, Muscle, Skeletal pathology, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Buttocks pathology, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse pathology, Muscle Neoplasms drug therapy, Muscle, Skeletal drug effects
- Published
- 2018
- Full Text
- View/download PDF
116. Efficacy of phototherapy in pityriasis lichenoides chronica of dark skin: A retrospective study from a tertiary center in Mexico.
- Author
-
Cuellar-Barboza A, Cardenas-De La Garza JA, Villarreal-Villarreal CD, Ocampo-Candiani J, Welsh O, Herz-Ruelas ME, and Gómez-Flores M
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Infant, Male, Mexico, Middle Aged, Pityriasis Lichenoides diagnosis, Pityriasis Lichenoides physiopathology, Remission Induction, Retrospective Studies, Skin pathology, Skin physiopathology, Treatment Outcome, Young Adult, Pityriasis Lichenoides radiotherapy, Skin radiation effects, Skin Pigmentation, Tertiary Care Centers, Ultraviolet Therapy
- Published
- 2018
- Full Text
- View/download PDF
117. The best cardiovascular risk calculator to predict carotid plaques in rheumatoid arthritis patients.
- Author
-
Wah-Suarez MI, Galarza-Delgado DA, Azpiri-Lopez JR, Colunga-Pedraza IJ, Cardenas-de la Garza JA, Vera-Pineda R, Arvizu-Rivera RI, Martinez-Moreno A, Ramos-Cazares RE, Abundis-Marquez EE, Guillen-Lozoya AH, Davila-Jimenez JA, Guillen-Gutierrez CY, and Elizondo-Riojas G
- Subjects
- Adult, Aged, Body Mass Index, Cardiovascular Diseases etiology, Carotid Artery Diseases etiology, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Female, Humans, Lipids blood, Male, Middle Aged, Risk Factors, Arthritis, Rheumatoid complications, Carotid Artery Diseases diagnostic imaging, Carotid Intima-Media Thickness
- Abstract
Atherosclerotic cardiovascular disease (ASCVD) is the leading cause of death in patients with rheumatoid arthritis (RA). Chronic inflammation and traditional risk factors increase cardiovascular risk (CVR) in these patients. Several CVR calculators are used in general population and in RA patients to predict cardiovascular outcomes and tailor therapy but the precision of these calculators in RA patients has yet to be determined. The aim of this study is to determine which risk calculator correlates best with carotid ultrasound (US) findings, specifically carotid plaque (CP) and carotid intima-media thickness (CIMT) in RA patients without clinical manifestations. This was a cross-sectional observational study relating CVR scores in RA patients with the presence of carotid US findings. A total of 97 patients 40 to 75 years old who fulfilled the 2010 ACR/EULAR and/or the 1987 ACR classification criteria for RA were selected. Clinical assessment of cardiovascular risk was performed using seven calculators and carotid US measurement of intima-media thickness and plaque. The tests with the highest sensitivity for CIMT were the Framingham BMI, Framingham lipids, ACC/AHA 2013, and QRISK2. In CP, the highest sensitivity was in QRISK2, SCORE, and ACC/AHA 2013. RA patients should be comprehensively evaluated to detect cardiovascular risk. Carotid US may be routinely recommended to detect subclinical atherosclerosis in RA patients. A lower cutoff point in CVR scales may be necessary to identify patients with a low and intermediate CVR to detect subclinical atherosclerosis earlier and personalize therapy.
- Published
- 2018
- Full Text
- View/download PDF
118. Implementing a Systematic Approach to Improve Governance and Deployment of Imaging Codes in Radiology.
- Author
-
Guimaraes CV, Smith LA, Garza JA, Blado ME, Lokey CS, and Donnelly LF
- Subjects
- Humans, International Classification of Diseases, Clinical Governance, Current Procedural Terminology, Quality Improvement, Radiology standards
- Abstract
Unlike medical procedure (Current Procedural Terminology [CPT]) and diagnostic (International Classification of Diseases [ICD]) codes, imaging (IMG) codes are defined at the local, institutional level. IMG codes are used within an organization to define IMG procedures and how they are routed between information systems. Our purpose is to describe the implementation of a reorganization (referred to as the IMG code cleanup) and governance structure deployed at a large children's hospital and the initial effects of that implementation. The number of IMG codes precleanup was 1388 and postcleanup was 826. This is a reduction by 40%. The mean number of addendums to radiology reports for billing reasons per month was 31.7 before and 18.6 after the IMG code cleanup. This represents a 41% decrease. We believe that the reorganization, standardization of the approach to naming and coding, reduction in the number of IMG codes, as well as governance structure put in place to maintain that organization has had both direct and indirect effects on the department's ability to both provide reliable IMG services and position the department to improve., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
119. Disseminated actinomycetoma due to Nocardia wallacei.
- Author
-
Welsh O, Salinas-Carmona MC, Brown-Elliott BA, Smith T, Cardenas-De La Garza JA, and Wallace RJ Jr
- Subjects
- Adolescent, Amputation, Surgical methods, Biopsy, Needle, Disease Progression, Drug Resistance, Multiple, Follow-Up Studies, Humans, Immunohistochemistry, Lower Extremity physiopathology, Male, Mexico, Microbial Sensitivity Tests, Mycetoma microbiology, Mycetoma surgery, Nocardia isolation & purification, Nocardia Infections diagnosis, Time Factors, Treatment Outcome, Anti-Bacterial Agents therapeutic use, Mycetoma diagnosis, Mycetoma drug therapy, Nocardia drug effects, Nocardia Infections drug therapy
- Abstract
Background: Actinomycetoma caused by Nocardia usually responds well to antibiotics. Emerging species of Nocardia, such as N. wallacei, can be a therapeutic challenge., Aims: Confirm the therapeutic effectivity of linezolid in multidrug resistant Nocardia Wallacei actinomycetoma., Materials and Methods: We evaluated the medical management of an 18-year-old man with multidrug resistant actinomycetoma of the left leg caused by N. transvalensis complex treated 17 years ago with linezolid 1200 mg a day. This bacteria was recently reclassified as Nocardia Wallacei by specific molecular biology technique., Results: The infection was cured after 3 months of treatment; the patient remained asymptomatic for the past 17 years. No adverse effects were found., Discussion: Frequently, strains of N. transvalensis complex have aminoglycoside resistance; in this case, we highlight the effectiveness of linezolid for the successful medical management of multidrug resistant actinomycetoma., Conclusion: Linezolid can be an alternative for the treatment of multidrug resistant Nocardia Wallacei., (© 2018 The International Society of Dermatology.)
- Published
- 2018
- Full Text
- View/download PDF
120. Unveiling a Drift Resistant Cryptotope within Marburgvirus Nucleoprotein Recognized by Llama Single-Domain Antibodies.
- Author
-
Garza JA, Taylor AB, Sherwood LJ, Hart PJ, and Hayhurst A
- Abstract
Marburg virus (MARV) is a highly lethal hemorrhagic fever virus that is increasingly re-emerging in Africa, has been imported to both Europe and the US, and is also a Tier 1 bioterror threat. As a negative sense RNA virus, MARV has error prone replication which can yield progeny capable of evading countermeasures. To evaluate this vulnerability, we sought to determine the epitopes of 4 llama single-domain antibodies (sdAbs or VHH) specific for nucleoprotein (NP), each capable of forming MARV monoclonal affinity reagent sandwich assays. Here, we show that all sdAb bound the C-terminal region of NP, which was produced recombinantly to derive X-ray crystal structures of the three best performing antibody-antigen complexes. The common epitope is a trio of alpha helices that form a novel asymmetric basin-like depression that accommodates each sdAb paratope via substantial complementarity-determining region (CDR) restructuring. Shared core contacts were complemented by unique accessory contacts on the sides and overlooks of the basin yielding very different approach routes for each sdAb to bind the antigen. The C-terminal region of MARV NP was unable to be crystallized alone and required engagement with sdAb to form crystals suggesting the antibodies acted as crystallization chaperones. While gross structural homology is apparent between the two most conserved helices of MARV and Ebolavirus , the positions and morphologies of the resulting basins were markedly different. Naturally occurring amino acid variations occurring in bat and human Marburgvirus strains all mapped to surfaces distant from the predicted sdAb contacts suggesting a vital role for the NP interface in virus replication. As an essential internal structural component potentially interfacing with a partner protein it is likely the C-terminal epitope remains hidden or "cryptic" until virion disruption occurs. Conservation of this epitope over 50 years of Marburgvirus evolution should make these sdAb useful foundations for diagnostics and therapeutics resistant to drift.
- Published
- 2017
- Full Text
- View/download PDF
121. Autohydrolysis pretreatment assessment in ethanol production from agave bagasse.
- Author
-
Rios-González LJ, Morales-Martínez TK, Rodríguez-Flores MF, Rodríguez-De la Garza JA, Castillo-Quiroz D, Castro-Montoya AJ, and Martinez A
- Subjects
- Cellulase, Ethanol, Fermentation, Hydrolysis, Agave, Cellulose
- Abstract
The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6w/v ratio (solid:liquid) and 200rpm. The solids obtained under the selected autohydrolysis conditions were subjected to enzymatic hydrolysis with a commercial cellulase cocktail, and the enzymatic hydrolysate was fermented using Saccharomyces cerevisiae. The results obtained from the pretreatment process showed that the glucan content in the pretreated solid was mostly preserved, and an increase in the digestibility was observed for the case with a SF of 4.13 (190°C, 30min). Enzymatic hydrolysis of the pretreated solids showed a yield of 74.3%, with a glucose concentration of 126g/L, resulting in 65.26g/L of ethanol after 10h of fermentation, which represent a 98.4% conversion according to the theoretical ethanol yield value., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
122. Prevalence of comorbidities in Mexican mestizo patients with rheumatoid arthritis.
- Author
-
Galarza-Delgado DA, Azpiri-Lopez JR, Colunga-Pedraza IJ, Cárdenas-de la Garza JA, Vera-Pineda R, Wah-Suárez M, Arvizu-Rivera RI, Martínez-Moreno A, Ramos-Cázares RE, Torres-Quintanilla FJ, Valdovinos-Bañuelos A, Esquivel-Valerio JA, and Garza-Elizondo MA
- Subjects
- Adult, Aged, Antirheumatic Agents therapeutic use, Arthritis, Rheumatoid diagnosis, Arthritis, Rheumatoid drug therapy, Comorbidity, Cross-Sectional Studies, Female, Humans, Male, Mexico epidemiology, Middle Aged, Prevalence, Prognosis, Prospective Studies, Risk Factors, Time Factors, Arthritis, Rheumatoid ethnology, Indians, North American
- Abstract
Patients with rheumatoid arthritis (RA) have a high risk for comorbid conditions which increase mortality, hospital admissions, costs of care and inability. To evaluate the prevalence of comorbidities in Mexican mestizo patients with RA and determine the associated risk factors. Cross-sectional study in which RA patients admitted to our outpatient clinic were consecutively enrolled. We collected data regarding demographics, disease characteristics and comorbidities at the time of the patient's visit to the clinic. We analyzed 225 patients. Their mean age was 55.7 ± 8.3 years; disease duration, 9.5 (3.8-15.5) years; female gender, 93.8%; Disease Activity Score using 28 joints-C-reactive protein, 3 (2-4); methotrexate use, 84.9%; use of any other conventional disease modifying anti-rheumatic drug, 65.7%; use of biological agents, 8%. The most frequently associated diseases were: hypertension, 29.8%; dyslipidemia, 27.1%; osteoporosis, 19.1%; diabetes, 12.4%; hypothyroidism, 6.2%; solid malignancies 4.4%. Risk factors were also evaluated, the most prevalent was overweight in 101 (44.9%) of our patients. A total of 71 (31.6%) had obesity. We also detected high blood pressure in 12.4%, hyperglycemia in 27.1% and hyperlipidemia in 49.8%. Due to the high frequency of comorbidities among RA patients, it is important to follow existing recommendations for their timely detection and management. Cardiovascular diseases must be evaluated with priority. The initial evaluation should include a thorough examination to prevent the deleterious effect of comorbidities in RA.
- Published
- 2017
- Full Text
- View/download PDF
123. Assessment of six cardiovascular risk calculators in Mexican mestizo patients with rheumatoid arthritis according to the EULAR 2015/2016 recommendations for cardiovascular risk management.
- Author
-
Galarza-Delgado DA, Azpiri-Lopez JR, Colunga-Pedraza IJ, Cardenas-de la Garza JA, Vera-Pineda R, Serna-Peña G, Arvizu-Rivera RI, Martinez-Moreno A, Wah-Suarez M, and Garza Elizondo MA
- Subjects
- Aged, Arthritis, Rheumatoid epidemiology, Cardiovascular Diseases epidemiology, Cross-Sectional Studies, Female, Humans, Male, Mexico epidemiology, Middle Aged, Risk Assessment methods, Arthritis, Rheumatoid complications, Cardiovascular Diseases etiology
- Abstract
Variability of the 10-year cardiovascular (CV) risk predicted by the Framingham Risk Score (FRS) using lipids, FRS using body mass index (BMI), Reynolds Risk Score (RRS), QRISK2, Extended Risk Score-Rheumatoid Arthritis (ERS-RA), and algorithm developed by the American College of Cardiology and the American Heart Association in 2013 (ACC/AHA 2013) according to the European League Against Rheumatism (EULAR) 2015/2016 update of its evidence-based recommendations for cardiovascular risk management in patients with rheumatoid arthritis (RA) has not been evaluated in Mexican mestizo patients. CV risk was predicted using six different risk calculators in 116 patients, aged 40-75, who fulfilled the ACR/EULAR 2010 classification criteria. Results were multiplied by 1.5 according to the EULAR 2015/2016 update. Global comparison of the risk predicted by all scales was done using the Friedman test, considering a P value of ≤0.05 as statistically significant. Individual comparison between the algorithms was made using the Wilcoxon signed-rank test, and a P value of ≤0.003 was considered statistically significant. All calculators showed to be different in the Friedman test (p ≤ 0.001). Median values of predicted 10-year CV risk were 11.02% (6.18-17.55) for FRS BMI; 8.47% (4.6-13.16) for FRS lipids; 5.55% (2.5-11.85) for QRISK2; 5% (3.1-8.65) for ERS-RA; 3.6% (1.5-9.3) for ACC/AHA 2013; and 1.5% (1.5-4.5) for RRS. ERS-RA showed no difference when compared against QRISK2 (p = 0.269). CV risk calculators showed variability among them and cannot be used indistinctly in RA-patients.
- Published
- 2017
- Full Text
- View/download PDF
124. Comparison of statin eligibility according to the Adult Treatment Panel III, ACC/AHA blood cholesterol guideline, and presence of carotid plaque by ultrasound in Mexican mestizo patients with rheumatoid arthritis.
- Author
-
Galarza-Delgado DA, Azpiri-Lopez JR, Colunga-Pedraza IJ, Cardenas-de la Garza JA, Vera-Pineda R, Garcia-Colunga JI, Arvizu-Rivera RI, Martinez-Moreno A, Villarreal-Perez JZ, Elizondo-Riojas G, and Garza Elizondo MA
- Subjects
- Aged, Atherosclerosis blood, Atherosclerosis complications, Atherosclerosis diagnostic imaging, Carotid Artery Diseases blood, Carotid Artery Diseases complications, Carotid Artery Diseases diagnostic imaging, Cross-Sectional Studies, Female, Humans, Male, Mexico, Middle Aged, Plaque, Atherosclerotic blood, Plaque, Atherosclerotic complications, Plaque, Atherosclerotic diagnostic imaging, Risk Assessment, Ultrasonography, Arthritis, Rheumatoid complications, Atherosclerosis drug therapy, Carotid Artery Diseases drug therapy, Cholesterol blood, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Plaque, Atherosclerotic drug therapy
- Abstract
Atherosclerotic cardiovascular disease (ASCVD) is the leading cause of death in rheumatoid arthritis (RA) patients. Guidelines of the American College of Cardiology and the American Heart Association (ACC/AHA) 2013 and the Adult Treatment Panel III (ATP-III) differ in their strategies to recommend initiation of statin therapy. The presence of carotid plaque (CP) by carotid ultrasound is an indication to begin statin therapy. We aimed to compare the recommendation to initiate statin therapy according to the ACC/AHA 2013 guidelines, ATP-III guidelines, and CP by carotid ultrasound. We then carried out an observational, cross-sectional study of 62 statin-naive Mexican mestizo RA patients, aged 40 to 75, who fulfilled the 1987 or 2010 ACR/European League Against Rheumatism (EULAR) classification criteria. CP was evaluated with B-mode ultrasound. Cohen's kappa (k) was used to assess agreement between ACC/AHA 2013 guidelines, ATP-III guidelines, and the presence of CP, considering a p < 0.05 as statistically significant. Agreement was classified as slight (0.01-0.20), fair (0.21-0.40), moderate (0.41-0.60), substantial (0.61-0.80), and an almost perfect agreement (0.81-1.00). Slight agreement (k = 0.096) was found when comparing statin recommendation between CP and ATP-III. Fair agreement (k = 0.242) was revealed between ACC/AHA 2013 and ATP-III. Comparison between ACC/AHA 2013 and CP showed moderate agreement (k = 0.438). ACC/AHA 2013 guidelines could be an adequate and cost-effective tool to evaluate the need of statin therapy in Mexican mestizo RA patients, with moderate agreement with the presence of CP by ultrasound.
- Published
- 2016
- Full Text
- View/download PDF
125. Biological effect on drug distribution and vascular healing via paclitaxel-coated balloon technology in drug eluting stent restenosis swine model.
- Author
-
Li Y, Tellez A, Rousselle SD, Dillon KN, Garza JA, Barry C, and Granada JF
- Subjects
- Animals, Cardiovascular Agents pharmacokinetics, Coronary Angiography, Coronary Restenosis diagnostic imaging, Coronary Restenosis etiology, Coronary Restenosis metabolism, Coronary Vessels diagnostic imaging, Coronary Vessels metabolism, Disease Models, Animal, Equipment and Supplies, Fibrin metabolism, Metals, Neointima, Paclitaxel pharmacokinetics, Percutaneous Coronary Intervention adverse effects, Swine, Tissue Distribution, Tomography, Optical Coherence, Cardiac Catheterization instrumentation, Cardiac Catheters, Cardiovascular Agents administration & dosage, Coated Materials, Biocompatible, Coronary Restenosis therapy, Coronary Vessels drug effects, Paclitaxel administration & dosage, Percutaneous Coronary Intervention instrumentation, Stents, Wound Healing drug effects
- Abstract
Objectives: To evaluate the biological effect of a paclitaxel-coated balloon (PCB) technology on vascular drug distribution and healing in drug eluting stent restenosis (DES-ISR) swine model., Background: The mechanism of action and healing response via PCB technology in DES-ISR is not completely understood., Methods: A total of 27 bare metal stents were implanted in coronary arteries and 30 days later the in-stent restenosis was treated with PCB. Treated segments were harvested at 1 hr, 14 days and 30 days post treatment for the pharmacokinetic analysis. In addition, 24 DES were implanted in coronary arteries for 30 days, then all DES-ISRs were treated with either PCB (n = 12) or uncoated balloon (n = 12). At day 60, vessels were harvested for histology following angiography and optical coherence tomography (OCT)., Results: The paclitaxel level in neointimal tissue was about 18 times higher (P = 0.0004) at 1 hr Cmax , and retained about five times higher (P = 0.008) at day 60 than that in vessel wall. A homogenous distribution of paclitaxel in ISR was demonstrated by using fluorescently labeled paclitaxel. Notably, in DES-ISR, both termination OCT and quantitative coronary angioplasty showed a significant neointimal reduction and less late lumen loss (P = 0.05 and P = 0.03, respectively) post PCB versus post uncoated balloon. The PES-ISR + PCB group displayed higher levels of peri-strut inflammation and fibrin scores compared to the -limus DES-ISR + PCB group., Conclusions: In ISR, paclitaxel is primarily deposited in neointimal tissue and effectively retained over time following PCB use. Despite the presence of metallic struts, a uniform distribution was characterized. PCB demonstrated an equivalent biological effect in DES-ISR without significantly increasing inflammation. © 2015 Wiley Periodicals, Inc., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
- Full Text
- View/download PDF
126. Safety and Clinical Outcome of the Delivery of Radiofrequency Nerve Ablation Therapy in a Renal Artery of Unusual Anatomy.
- Author
-
de Leon-Martinez EP, Garza JA, Azpiri-Lopez JR, Dillon KN, Salazar LO, Canepa-Campos F, Rousselle SD, and Tellez A
- Subjects
- Antihypertensive Agents therapeutic use, Drug Resistance, Humans, Hypertension diagnosis, Hypertension physiopathology, Male, Middle Aged, Renal Artery diagnostic imaging, Renal Artery innervation, Tomography, X-Ray Computed, Treatment Outcome, Blood Pressure drug effects, Catheter Ablation, Hypertension surgery, Kidney blood supply, Renal Artery abnormalities, Renal Artery surgery, Sympathectomy methods
- Abstract
Introduction: Catheter-based renal sympathetic denervation is an emerging therapy for resistant hypertension (RHTN) patients, resulting in a significant blood pressure reduction. The presence of accessory renal arteries and anomalous branching patterns are reported in approximately 20-27 % of patients. However, accessory renal arteries, when smaller than 4 mm in diameter, they are out of the inclusion criteria for renal denervation therapy. For this reason patients with evidence of accessory renal arteries have been excluded in previous clinical trials. Recent data suggest that accessory renal arteries may play an important role in non-response therapy when they do not receive renal denervation treatment., Case Report: In this report, we present the outcome of a patient with resistant hypertension and an anomalous right renal artery, having undergone denervation of both principal and accessory renal arteries. The renal ablation by radiofrequency energy of a distant accessory renal artery resulted in a safe procedure with no clinical complications., Conclusion: Consistent with literature the RDN of all, main and accessory renal arteries, was effective in decreasing patient blood pressure while decreasing the need for antihypertensive medication.
- Published
- 2015
- Full Text
- View/download PDF
127. Neuromatous regeneration as a nerve response after catheter-based renal denervation therapy in a large animal model: immunohistochemical study.
- Author
-
Rousselle SD, Brants IK, Sakaoka A, Hubbard B, Jackson ND, Wicks JR, Dillon KN, Naiche LA, Hart R, Garza JA, and Tellez A
- Subjects
- Animals, Biomarkers metabolism, Female, GAP-43 Protein metabolism, Models, Animal, S100 Proteins metabolism, Sus scrofa, Tyrosine 3-Monooxygenase metabolism, Catheter Ablation methods, Denervation, Kidney innervation, Nerve Regeneration physiology, Sympathetic Nervous System physiology
- Abstract
Background: Renal denervation (RDN) emerged as a therapeutic option for resistant hypertension. Nerve regrowth after RDN has been questioned. We aimed to characterize the nerve response after RDN., Methods and Results: Swine underwent bilateral RDN and were followed up for 7, 30, and 90 days and evaluated with S100 (Schwann cell), tyrosine hydroxylase (TH; efferent nerves), and growth-associated protein 43 (neurite regeneration) markers. At 7 days, nerve changes consisted of necrosis associated with perineurial fibrosis and distal atrophy with inflammation. At 30 days changes were substituted by healing changes (ie, fibrosis). This response progressed through 90 days resulting in prominent neuroma formation. Immunohistochemistry at 7 days: TH staining was strongly decreased in treated nerves. Early regenerative attempts were observed with strongly TH and growth-associated protein 43 positive and weak S100 disorganized nerve sprouts within the thickened perineurium. Distal atrophic nerves show weak staining for all 3 markers. At 30 days, affected nerves show a weak TH and S100 staining. Evident growth-associated protein 43+ disorganized neuromatous tangles in the thickened perineurium of severed nerves were observed. At 90 days, some TH expression was observed together with prominent S100+ and growth-associated protein 43+ neuromatous tangles with disorganized architecture. The potential for regenerative activity is unlikely based on the disrupted architecture of these neuromatous tangles at the radiofrequency lesion sites., Conclusions: This study is the first documentation that a progressive regenerative response occurs as early as 7 days after RDN, resulting in a poorly organized neuromatous regeneration. This finding is of paramount importance to further establish the potential functional significance of a regeneration after RDN., (© 2015 American Heart Association, Inc.)
- Published
- 2015
- Full Text
- View/download PDF
128. Peri-strut low-intensity areas in optical coherence tomography correlate with peri-strut inflammation and neointimal proliferation: an in-vivo correlation study in the familial hypercholesterolemic coronary swine model of in-stent restenosis.
- Author
-
Tellez A, Afari ME, Buszman PP, Seifert P, Cheng Y, Milewski K, McGregor JC, Garza JA, Roberts MB, Yi GH, Kaluza GL, and Granada JF
- Subjects
- Animals, Coronary Restenosis metabolism, Coronary Vessels metabolism, Disease Models, Animal, Drug-Eluting Stents, Fibrin metabolism, Graft Occlusion, Vascular metabolism, Hyperplasia, Inflammation metabolism, Male, Neointima metabolism, Stents, Swine, Coronary Artery Disease, Coronary Restenosis pathology, Coronary Vessels pathology, Graft Occlusion, Vascular pathology, Hyperlipoproteinemia Type II, Inflammation pathology, Neointima pathology, Tomography, Optical Coherence
- Abstract
Background: Peri-strut low-intensity area (PLI) is a common imaging finding during the evaluation of in-stent neointima using optical coherence tomography (OCT). We aimed to determine the biological significance of PLI by comparing in-vivo OCT images with the corresponding histological sections obtained from the familial hypercholesterolemic swine model of coronary stenosis., Methods: A total of 26 coronary vessels of nine familial hypercholesterolemic swine were injured with 30% balloon overstretch and then immediately followed by everolimus eluting or bare metal stent placement at 20% overstretch. At 30 days, all stented vessels were subjected to in-vivo OCT analysis and were harvested for histological evaluation. For OCT analysis, stent cross-sections (three per stent) were categorized into presence (PLI+) or absence (PLI-) of PLI. In histology, inflammation and fibrin deposition were scored semiquantitatively from 0 (none) to 3 (severe)., Results: PLI was found in 64.9% of stent sections. Peri-strut inflammation was more frequently observed in OCT sections PLI (+) compared with PLI (-) (56.0 vs. 7.4%, P=0.01). In contrast, peri-strut fibrin deposits was similar in both groups (PLI+=58.0% vs. PLI-=59.3%, P=0.94). Histological neointimal thickness was significantly higher in PLI (+) sections (mean±SE: 0.68±0.06 vs. 0.34±0.02 mm; P<0.01), yielding a higher percent area stenosis compared with PLI (-) (mean±SE: 59.0±4.4 vs. 34.1±2.2%, P<0.01). The PLI diagnostic sensitivity and specificity for inflammation were 80 and 76.1%, respectively (>56% PLI, area under the curve=0.86, P<0.01), whereas for fibrin deposition, the sensitivity and specificity were 42.2 and 76.1%, respectively (area under the curve=0.56, P=NS). Area under the receiver operating characteristic curve was significantly higher for identifying inflammation than fibrin (0.86 vs. 0.56, P<0.01). The severity of PLI correlated with the neointimal thickness when assessed by OCT (R=0.79, P<0.001)., Conclusion: The presence of PLI in OCT correlates with neointimal thickness and appears to have a diagnostic value in the recognition of peri-strut inflammation, therefore possibly serving as a surrogate for in-vivo assessment of stent efficacy.
- Published
- 2014
- Full Text
- View/download PDF
129. Female breast symptoms in patients attended in the family medicine practice.
- Author
-
González-Pérez B, Salas-Flores R, Sosa-López ML, Barrientos-Guerrero CE, Hernández-Aguilar CM, Gómez-Contreras DE, and Sánchez-Garza JA
- Subjects
- Adult, Aged, Family Practice, Female, Humans, Middle Aged, Primary Health Care, Young Adult, Breast Diseases diagnosis, Breast Neoplasms diagnosis
- Abstract
Background: there are few studies on breast symptoms (BS) in patients attended at primary care units in Mexico. The aim was to determine the frequency and types of BS overall and by age-group and establish which BS were related to diagnosis of breast cancer., Methods: data from all female patients with a breast-disease-related diagnosis, attended from 2006 to 2010, at the Family Medicine Unit 38, were collected. The frequencies of BS were determined by four age-groups (< 19, 20-49, 50-69, > 70 years) and likelihood ratios for breast cancer for each breast-related symptom patient, with a 95 % confidence interval (CI)., Results: the most frequent BS in the study population were lump/mass (71.7 %) and breast pain (67.7 %) of all breast complaints, and they were more noted in women age group of 20-49 years. Overall, 120 women had breast cancer diagnosed with a median age of 53.51 + 12.7 years. Breast lump/mass had positive likelihood ratios for breast cancer 4.53 (95 % CI = 2.51-8.17) and breast pain had increased negative LR = 1.08 (95 % CI = 1.05-1.11)., Conclusions: breast lump/mass was the predominant presenting complaint among females with breast symptoms in our primary care unit, and it was associated with elevated positive likelihood of breast cancer.
- Published
- 2013
130. First reported case of pneumonia caused by Cedecea lapagei in America.
- Author
-
Lopez LA, Ibarra BS, de la Garza JA, Rada Fde J, Nuñez AI, and López MG
- Subjects
- Adult, Enterobacteriaceae classification, Enterobacteriaceae Infections diagnosis, Enterobacteriaceae Infections epidemiology, Humans, Leukemia, Promyelocytic, Acute complications, Male, Mexico epidemiology, Pneumonia, Bacterial diagnosis, Pneumonia, Bacterial epidemiology, Enterobacteriaceae isolation & purification, Enterobacteriaceae Infections microbiology, Pneumonia, Bacterial microbiology
- Abstract
Cedecea represents a genus in the Enterobacteriaceae family that has been rarely associated with human infection. The clinical relevance of Cedecea lapagei has yet to be elucidated. This is the first reported case of pneumonia due to C. lapagei in a patient with acute promyelocytic leukemia., (Copyright © 2013 Elsevier Editora Ltda. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
131. Timing of the onset of acute respiratory distress syndrome: a population-based study.
- Author
-
Shari G, Kojicic M, Li G, Cartin-Ceba R, Alvarez CT, Kashyap R, Dong Y, Poulose JT, Herasevich V, Garza JA, and Gajic O
- Subjects
- Age of Onset, Aged, Aged, 80 and over, Female, Follow-Up Studies, Humans, Male, Middle Aged, Minnesota epidemiology, Retrospective Studies, Risk Factors, Time Factors, Population Surveillance, Respiratory Distress Syndrome epidemiology
- Abstract
Background: Many patients with acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) have had recent healthcare interventions prior to developing ALI/ARDS., Objective: To determine the timing of ALI/ARDS onset in relation to hospital admission and other healthcare interventions., Methods: We conducted a population-based observational cohort study with a validated electronic surveillance tool, and identified patients with possible ALI/ARDS among critically ill adults at Mayo Clinic hospitals that provide critical care services for Olmsted County, Minnesota, in 2006. Trained investigators independently reviewed electronic medical records and confirmed the presence and timing of ALI/ARDS based on the American-European consensus definition., Results: Of 124 episodes of ALI in 118 patients, only 5 did not fulfill the ARDS criteria. The syndrome developed a median 30 hours (IQR 10-82 h) after hospital admission in 79 patients (67%). ARDS was present on admission in 39 patients (33%), of whom 14 had recent hospitalization, 6 were transferred from nursing homes, and 3 had recent out-patient contact (1 antibiotic prescription, 1 surgical intervention, and 1 chemotherapy). Only 16 ARDS patients (14%) did not have known recent contact with a healthcare system. Compared to ARDS on admission, hospital-acquired ARDS was more likely to occur in surgery patients (54% vs 15%, P < .001), and had longer adjusted hospital stay (mean difference 8.9 d, 95% CI 0.3-17.4, P = .04)., Conclusions: ARDS in the community most often develops either during hospitalization or in patients who recently had contact with a healthcare system. These findings have important implications for potential preventive strategies.
- Published
- 2011
- Full Text
- View/download PDF
132. [Upper GI tract diseases II. Eosinophilic esophagitis].
- Author
-
Madrazo de la Garza JA
- Subjects
- Eosinophilic Esophagitis therapy, Esophagus pathology, Humans, Eosinophilic Esophagitis pathology
- Published
- 2010
133. Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents.
- Author
-
Conway JO, Sherwood LJ, Collazo MT, Garza JA, and Hayhurst A
- Subjects
- Animals, Antibodies, Neutralizing immunology, Camelids, New World, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Antibodies immunology, Antibody Specificity, Botulinum Toxins immunology, Indicators and Reagents
- Abstract
Background: There are currently 7 known serotypes of botulinum neurotoxin (BoNT) classified upon non-cross reactivity of neutralizing immunoglobulins. Non-neutralizing immunoglobulins, however, can exhibit cross-reactivities between 2 or more serotypes, particularly mosaic forms, which can hamper the development of highly specific immunoassays, especially if based on polyclonal antisera. Here we employ facile recombinant antibody technology to subtractively select ligands to each of the 7 BoNT serotypes, resulting in populations with very high specificity for their intended serotype., Methods and Findings: A single llama was immunized with a cocktail of 7 BoNT toxoids to generate a phage display library of single domain antibodies (sdAb, VHH or nanobodies) which were selected on live toxins. Resulting sdAb were capable of detecting both toxin and toxin complex with the best combinations able to detect 100s-10s of pg per 50 microL sample in a liquid bead array. The most sensitive sdAb were combined in a heptaplex assay to identify each of the BoNT serotypes in buffer and milk and to a lesser extent in carrot juice, orange juice and cola. Several anti-A(1) sdAb recognized A2 complex, showing that subtype cross-reactivity within a serotype was evident. Many of our sdAb could act as both captor and tracer for several toxin and toxin complexes suggesting sdAb can be used as architectural probes to indicate BoNT oligomerisation. Six of 14 anti-A clones exhibited inhibition of SNAP-25 cleavage in the neuro-2A assay indicating some sdAb had toxin neutralizing capabilities. Many sdAb were also shown to be refoldable after exposure to high temperatures in contrast to polyclonal antisera, as monitored by circular dichroism., Conclusions: Our panel of molecularly flexible antibodies should not only serve as a good starting point for ruggedizing assays and inhibitors, but enable the intricate architectures of BoNT toxins and complexes to be probed more extensively.
- Published
- 2010
- Full Text
- View/download PDF
134. Kinetic, dynamic, ligand binding properties, and structural models of a dual-substrate specific nudix hydrolase from Schizosaccharomyces pombe.
- Author
-
Garza JA, Ilangovan U, Hinck AP, and Barnes LD
- Subjects
- Amino Acid Sequence, Binding Sites, Biocatalysis, Catalytic Domain, Dinucleoside Phosphates chemistry, Dinucleoside Phosphates metabolism, Gene Deletion, Inositol Phosphates chemistry, Inositol Phosphates metabolism, Kinetics, Magnesium Chloride chemistry, Models, Molecular, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Phytic Acid chemistry, Phytic Acid metabolism, Protein Binding, Protein Conformation, Protein Structure, Secondary, Pyrophosphatases genetics, Pyrophosphatases metabolism, Recombinant Proteins chemistry, Schizosaccharomyces enzymology, Schizosaccharomyces pombe Proteins genetics, Schizosaccharomyces pombe Proteins metabolism, Sequence Homology, Amino Acid, Titrimetry, Pyrophosphatases chemistry, Schizosaccharomyces pombe Proteins chemistry
- Abstract
Schizosaccharomyces pombe Aps1 is a nudix hydrolase that catalyzes the hydrolysis of both diadenosine 5',5'''-P(1),P(n)-oligophosphates and diphosphoinositol polyphosphates in vitro. Nudix hydrolases act upon a wide variety of substrates, despite having a common 23 amino acid catalytic motif; hence, the residues responsible for substrate specificity are considered to reside outside the common catalytic nudix motif. The specific residues involved in binding each substrate of S. pombe Aps1 are unknown. In this study, we have conducted mutational and kinetic studies in combination with structural homology modeling and NMR spectroscopic analyses to identify potential residues involved in binding each class of substrates. This study demonstrates several major findings with regard to Aps1. First, the determination of the kinetic parameters of K(m) and k(cat) indicated that the initial 31 residues of Aps1 are not involved in substrate binding or catalysis with respect to Ap(6)A. Second, NMR spectroscopic analyses revealed the secondary structure and three dynamic backbone regions, one of which corresponds to a large insert in Aps1 as compared to other putative fungal orthologues. Third, two structural models of Aps1Delta2-19, based on the crystal structures of human DIPP1 and T. thermophilus Ndx1, were generated using homology modeling. The structural models were in excellent agreement with the NMR-derived secondary structure of Aps1Delta2-19. Fourth, NMR chemical shift mapping in conjunction with structural homology models indicated several residues outside the catalytic nudix motif that are involved in specific binding of diphosphoinositol polyphosphate or diadenosine oligophosphate ligands.
- Published
- 2009
- Full Text
- View/download PDF
135. [Digestive system functional disorders in children].
- Author
-
Madrazo de la Garza JA
- Subjects
- Child, Digestive System Diseases classification, Digestive System Diseases etiology, Guidelines as Topic, Humans, Digestive System Diseases diagnosis
- Published
- 2006
136. Burnout in residency: a statewide study.
- Author
-
Garza JA, Schneider KM, Promecene P, and Monga M
- Subjects
- Depersonalization epidemiology, Female, Gynecology education, Humans, Male, Obstetrics education, Prevalence, Surveys and Questionnaires, Texas epidemiology, Burnout, Professional epidemiology, Internship and Residency
- Abstract
Objective: To determine the prevalence of burnout in residents in obstetrics and gynecology through the use of a validated tool., Methods: The Maslach Burnout Inventory Human Services questionnaire is a previously validated tool that measures burnout. Obstetrics and gynecology residents from Texas were invited to participate in this study in 2002. The Maslach Burnout Inventory Human Services questionnaire and a demographic survey were distributed to each resident. Responses were anonymous and returned by mail. Contingency coefficient and chi2 tests were used for analysis; values of P < 0.05 were significant., Results: Residents (n = 368) from 17 programs in Texas were surveyed. Responses were received from 14 programs (82.4%), with 136 surveys (37%) returned. Overall, 38.2% reported high emotional exhaustion, 47.1% reported high depersonalization, and 19.1% reported reduced personal accomplishment. The number of residents experiencing true burnout (high emotional exhaustion, high depersonalization, and low personal accomplishment) was 17.6% (n = 24)., Conclusions: High levels of emotional exhaustion and depersonalization occur in some residents. Burnout in residents included in this study was approximately 18%.
- Published
- 2004
- Full Text
- View/download PDF
137. Potential for Dispersal of Fusarium oxysporum f. sp. erythroxyli by Infested Seed.
- Author
-
Gracia-Garza JA, Fravel DR, Nelson AJ, Elias KS, Bailey BA, Gardini EA, and Darlington LC
- Abstract
Fusarium oxysporum f. sp. erythroxyli causes a vascular wilt of the narcotic plant coca (Erythroxylum coca var. coca). To determine whether this pathogen can be transmitted by infested seed, fruit from symptomatic and asymptomatic plants was collected from different coca-growing areas in Peru and from an experimental field site in Hawaii. A total of 202 fruit from Peru and 69 fruit from Hawaii were surface-disinfested and separated into five parts: pedicel, pericarp, seed coat, endosperm, and cotyledons. After the pedicel and pericarp were removed from the seed coat, the seed was surface disinfested again. Each fruit part was plated separately. Both F. oxysporum and F. moniliforme were recovered from fruit collected in Peru. Both species were isolated from all parts of some fruit. F. oxysporum was isolated from 33% of the fruit plated and most (35%) of these isolates were obtained from the seed coat. Slightly greater numbers of isolates (57%) were recovered from asymptomatic plants than from symptomatic plants (43%). Only F. oxysporum was isolated from fruit collected in Hawaii. Most of these isolates (59%) were from the pedicels of fruit collected from symptomatic plants. Out of 91 isolates of F. oxysporum, 21 were pathogenic to coca seedlings in a bioassay. Six of these pathogenic isolates were originally from the pedicel of the fruit, eight from the pericarp, four from the seed coat, and three from the endosperm. No isolates from the cotyledons were pathogenic. Most of the pathogenic isolates (76%) were from symptomatic plants. The pathogenic isolates were characterized using random amplified polymorphic DNA analysis and vegetative compatibility groups. Based on these analyses, two different subpopulations of the forma specialis erythroxyli were found in Peru, whereas only one was present in Hawaii. These data indicate that infested seed may contribute significantly to dissemination of this pathogen because seed is collected by growers and planted fresh or fermented briefly before planting.
- Published
- 1999
- Full Text
- View/download PDF
138. Effect of Relative Humidity on Sporulation of Fusarium oxysporum in Various Formulations and Effect of Water on Spore Movement Through Soil.
- Author
-
Gracia-Garza JA and Fravel DR
- Abstract
ABSTRACT Fusarium oxysporum f. sp. erythroxyli is being investigated as a mycoherbicide for the narcotic plant coca. Sporulation of the fungus in seven formulations containing different organic substrates and movement of its propagules through soil were studied. The formulations were a granular wheat flour/kaolin (pesta); an extruded wheat and rice flour (C-6); and five alginate pellet products containing corn cobs, soybean hull fiber, canola meal, rice flour, or rice flour plus canola oil. Formulations were incubated at 25 degrees C for 6 weeks in desiccators with various salt solutions to provide nine relative humidities (RH), ranging from 100% (pure deionized water) to 0% (anhydrous (CaSO(4)). Hyphae of F. oxysporum f. sp. erythroxyli grew out of alginate pellets with canola meal, rice, and rice plus canola oil as early as 24 h at 100% constant RH. Alginate pellets of rice plus canola oil and granular C-6 and pesta formulations consistently produced more microconidia, macroconidia, and CFU than the other four formulations at all RH tested. The C-6 formulation produced more propagules than the other formulations at low RH (<53%). Canola meal pellets produced more spores than three other formulations when exposed to fluctuating RH (100 to 75%). The effect of percolating water on spore movement through soil was studied using a plant-pathogenic isolate of F. oxysporum f. sp. niveum. To determine the effect of water percolation on propagule movement, formulations were placed on soil columns and artificial rain was applied. In general, 10-fold fewer CFU were recovered at a 8- to 10-cm depth compared with a 0- to 2-cm depth.
- Published
- 1998
- Full Text
- View/download PDF
139. Dispersal of Formulations of Fusarium oxysporum f. sp. erythroxyli and F. oxysporum f. sp. melonis by Ants.
- Author
-
Gracia-Garza JA, Fravel DR, Bailey BA, and Hebbar PK
- Abstract
ABSTRACT A natural epidemic of Fusarium wilt on coca (Erythroxylum coca) in Peru prompted the suggestion of possibly using the pathogen Fusarium oxysporum f. sp. erythroxyli as a mycoherbicide against this narcotic plant. During field trials conducted in Kauai, HI, to test the pathogenicity of the coca wilt pathogen, ants were observed removing formulations from test plots. While removal of formulations by ants was considered detrimental with respect to conducting field tests, ant removal was considered potentially beneficial in disseminating the mycoherbicide. Thus, research was initiated to assess the ability of formulation additives to alter the preference of ants for the formulated mycoherbicide. In Hawaii, preference of indigenous ants for removing formulations was tested using three different food bases (rice, rice plus canola oil, and wheat flour [gluten]). Similar tests were conducted at Beltsville, MD, using F. oxysporum f. sp. melonis, in which the formulation based on wheat flour was replaced by a formulation based on canola meal. Formulations based on wheat were preferred by ants in both locations; up to 90% of the wheat plus rice flour granules (C-6) and the wheat gluten plus kaolin granules (pesta) were removed within 24 h, while only 20% of those containing rice without oils were taken. However, when either canola, sunflower (Maryland only), or olive oil was added to the rice formulation, up to 90% of the granules were taken. The formulation based on canola meal was less attractive to ants, as only 65% of the granules were removed within a period of 24 h. Ants showed no preference with respect to presence or absence of fungal biomass. To alter the attractiveness of the C-6 formulation to ants, C-6 was amended with three natural products. Canna and tansy leaves were added to C-6 at a ratio of 1:5 (wt/wt), while chili powder was added at 1:25 or 1:2.5 (wt/wt). Canna, tansy, and the higher rate of chili powder significantly reduced the number of C-6 granules removed by ants. Canna and tansy leaves affected neither germination nor sporulation of the mycoherbicide, while the high concentration of chili powder reduced viability of propagules in the formulation. More F. oxysporum f. sp. erythroxyli-type colonies were recovered from inside ant nests (9 cm depth) than from nest surfaces, indicating that ants may distribute the mycoherbicide in the soil profile. Ants passively carried propagules of F. oxysporum f. sp. erythroxyli outside their bodies, as well as either very closely adhering to the outside or within their bodies.
- Published
- 1998
- Full Text
- View/download PDF
140. [Constipation in the pediatric patient].
- Author
-
Madrazo de la Garza JA
- Subjects
- Child, Humans, Constipation diagnosis, Constipation etiology, Constipation therapy
- Published
- 1996
141. [Eradication of Helicobacter pylori and chronic abdominal pain].
- Author
-
Madrazo de la Garza JA
- Subjects
- Adolescent, Anti-Ulcer Agents administration & dosage, Child, Drug Therapy, Combination administration & dosage, Humans, Omeprazole administration & dosage, Abdominal Pain drug therapy, Anti-Bacterial Agents, Anti-Ulcer Agents pharmacology, Drug Therapy, Combination pharmacology, Helicobacter Infections drug therapy, Helicobacter pylori drug effects, Omeprazole pharmacology
- Published
- 1996
142. [Gastroesophageal reflux in pediatrics].
- Author
-
Madrazo de la Garza JA and González Ortiz B
- Subjects
- Age Factors, Child, Diagnosis, Differential, Diet, Esophagitis, Peptic diagnosis, Esophagitis, Peptic etiology, Esophagitis, Peptic therapy, Humans, Gastroesophageal Reflux diagnosis, Gastroesophageal Reflux therapy
- Abstract
The frequency of gastroesophageal reflux disease in pediatrics has increased. There is not a clear explanation, some believe there is more awareness of the disease, others believe that new formulas, which are richer in nutrients, may irritate the gastrointestinal tract of the infant. Clinically, children present with regurgitation-malnutrition, respiratory disease, and esophagitis. The medical treatment aims to improve the eating techniques, to decrease the gastric acid output, and to improve the motility function of the esophago-gastrointestinal tract. Surgical treatment is rarely needed.
- Published
- 1994
143. A new direct pancreatic function test in pediatrics.
- Author
-
Madrazo-de la Garza JA, Gotthold M, Lu RB, Hill ID, and Lebenthal E
- Subjects
- Child, Preschool, Cholecystokinin, Duodenum enzymology, Endoscopy, Exocrine Pancreatic Insufficiency diagnosis, Fiber Optic Technology, Humans, Intestinal Secretions chemistry, Male, Pancreatic Function Tests standards, Secretin, Suction, Pancreatic Function Tests methods, Pediatrics
- Abstract
Traditional methods for collecting duodenal fluid are time consuming and technically difficult. A simple endoscopic method is proposed in this report as a means of collecting duodenal fluid to perform exocrine pancreatic function tests. Thirty-five patients between 24 and 36 months of age were studied for pancreatic exocrine function. Twenty-seven presented with chronic diarrhea and 8 with failure to thrive. In 20 patients (group 1), duodenal fluid was collected by means of a double-lumen tube and sequential administration of pancreozymin (PZN) and secretin (SEC). The rest (group 2) had duodenal aspiration from the level of the papilla of Vater through a fiberoptic endoscope following administration of SEC only. The procedure took approximately 3 h in group 1 and 45 min in group 2. Secretin administration produced comparable levels of enzymes in both groups. Pancreozymin produced the highest enzyme levels, but this was only significantly higher than SEC-induced levels in the case of lipase. Endoscopic collection of duodenal fluid following SEC administration is a safe, quick, and reliable method of collecting pancreatic secretion.
- Published
- 1991
- Full Text
- View/download PDF
144. Brain-stem tuberculoma.
- Author
-
Rangel-Guerra RA, Martinez HR, Garza JA, and Ancer J
- Subjects
- Brain Diseases diagnosis, Diagnosis, Differential, Humans, Brain Stem, Tuberculoma diagnosis
- Published
- 1991
- Full Text
- View/download PDF
145. Massive fat and necrotic bone marrow embolization in a previously undiagnosed patient with sickle cell disease.
- Author
-
Garza JA
- Subjects
- Adult, Anaphylaxis diagnosis, Anemia, Sickle Cell complications, Anemia, Sickle Cell pathology, Diagnosis, Differential, Disseminated Intravascular Coagulation etiology, Disseminated Intravascular Coagulation pathology, Embolism etiology, Embolism, Fat etiology, Female, Humans, Infarction complications, Infarction pathology, Iron administration & dosage, Necrosis, Anemia, Sickle Cell diagnosis, Bone Marrow pathology, Embolism pathology, Embolism, Fat pathology
- Abstract
A case of sickle cell disease diagnosed postmortem is described. A 37-year-old black woman presented with anemia, respiratory distress, and abdominal and back pain. Death followed an intramuscular injection of iron, and anaphylaxis was clinically diagnosed. At autopsy, massive fat and necrotic bone marrow embolization of pulmonary and renal vessels was found. In the vertebral column, multifocal areas of ischemic necrosis were present, and proved to be the source of this embolization. Sickled red cells appeared in bone marrow sinusoids, and signs of disseminated intravascular coagulation were present.
- Published
- 1990
- Full Text
- View/download PDF
146. Population genetics in the state of Nuevo León, México. VI. Frequencies of ABO, Rh(D), MN and other genetic traits among normal and partially colour-blind males.
- Author
-
Garza-Chapa R, Villarreal-Garza JA, Leal-Garza CH, and Cerda-Flores RM
- Subjects
- Blood Group Antigens genetics, Gene Frequency, Genetic Markers, Humans, Indians, North American, Male, Mexico, Spain ethnology, Color Vision Defects genetics
- Published
- 1983
147. Response of exocrine pancreas to corticosterone and aldosterone after adrenalectomy.
- Author
-
Alliet P, Lu RB, Madrazo de la Garza JA, Santer R, Lebenthal E, and Lee PC
- Subjects
- Adrenal Glands physiology, Adrenalectomy, Amylases metabolism, Animals, Body Weight, Female, Pancreas enzymology, Rats, Rats, Inbred Strains, Spironolactone metabolism, Aldosterone physiology, Corticosterone physiology, Pancreas physiology
- Abstract
The long-term effect of adrenalectomy (Adx) on the exocrine pancreas was examined in female adult rats. Pancreatic amylase concentration decrease to 50% of the control level starting 10 days after Adx, whereas the levels of trypsinogen and lipase remained unchanged. Replacement studies beginning 24 h after surgery with corticosterone (B, 1 mg/100 g body wt) or aldosterone (ALDO, 8 micrograms/100 g body wt) alone did not prevent the decline in amylase after Adx. However, when both hormones were administered together, pancreatic amylase concentration was maintained at a level similar to that of the control group. Serum corticosterone levels in the rats receiving B alone or B + ALDO were not different, and were comparable to levels found in normal rats. Both ALDO and B, given for 5 days starting 10 days after Adx, were required to restore amylase concentrations toward control values. When spironolactone (SPIRO, 3 mg/100 g body wt), a specific mineralocorticoid receptor blocker was administered bid together with ALDO + B, it blocked the increase in pancreatic amylase seen in ALDO + B treated rats but did not affect the serum corticosterone levels. These results suggest that mineralocorticoids are also involved in modulating the level of amylase in the rat exocrine pancreas.
- Published
- 1989
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.