101. Nuclear Speckle RNA Binding Proteins Remodel Alternative Splicing and the Non-coding Arabidopsis Transcriptome to Regulate a Cross-Talk Between Auxin and Immune Responses
- Author
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Federico Ariel, Thomas Blein, Richard Rigo, Martin Crespi, Celine Charon, Natali Romero, Jérémie Bazin, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Centre National de Génotypage (CNG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Instituto de Agrobiotecnología del Litoral [Santa Fe] (IAL), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Universidad Nacional del Litoral [Santa Fe] (UNL), Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), ANR-10-LABX-40, ANR grant SPLISIL, Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Bazin, Jérémie, and Crespi, Martin
- Subjects
0106 biological sciences ,0301 basic medicine ,Polyadenylation ,RNA-binding protein ,AUXIN ,Plant Science ,RNA binding proteins ,RNP complexes ,alternative splicing ,immune response ,auxin ,lcsh:Plant culture ,Biology ,01 natural sciences ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,03 medical and health sciences ,IMMUNE RESPONSE ,Gene expression ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,lcsh:SB1-1110 ,ALTERNATIVE SPLICING ,purl.org/becyt/ford/1.6 [https] ,Gene ,Lateral root formation ,ComputingMilieux_MISCELLANEOUS ,Original Research ,Alternative splicing ,RNA ,Bioquímica y Biología Molecular ,Cell biology ,RNA BINDING PROTEINS ,030104 developmental biology ,RNA splicing ,CIENCIAS NATURALES Y EXACTAS ,RNP COMPLEXES ,010606 plant biology & botany - Abstract
Nuclear speckle RNA binding proteins (NSRs) act as regulators of alternative splicing (AS) and auxin-regulated developmental processes such as lateral root formation in Arabidopsis thaliana. These proteins were shown to interact with specific alternatively spliced mRNA targets and at least with one structured lncRNA, named Alternative Splicing Competitor RNA. Here, we used genome-wide analysis of RNAseq to monitor the NSR global role on multiple tiers of gene expression, including RNA processing and AS. NSRs affect AS of 100s of genes as well as the abundance of lncRNAs particularly in response to auxin. Among them, the FPA floral regulator displayed alternative polyadenylation and differential expression of antisense COOLAIR lncRNAs in nsra/b mutants. This may explains the early flowering phenotype observed in nsra and nsra/b mutants. GO enrichment analysis of affected lines revealed a novel link of NSRs with the immune response pathway. A RIP-seq approach on an NSRa fusion protein in mutant background identified that lncRNAs are privileged direct targets of NSRs in addition to specific AS mRNAs. The interplay of lncRNAs and AS mRNAs in NSR-containing complexes may control the crosstalk between auxin and the immune response pathway. Fil: Bazin, Jérémie. Universite Paris-sud Xi; Fil: Romero, Natali. Universite Paris-sud Xi; Fil: Rigo, Richard. Universite Paris-sud Xi; Fil: Charon, Celine. Universite Paris-sud Xi; Fil: Blein, Thomas. Universite Paris-sud Xi; Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Crespi, Martin. Universite Paris-sud Xi
- Published
- 2018