51. Dentonin, a Fragment of MEPE, Enhanced Dental Pulp Stem Cell Proliferation
- Author
-
C. Gao, Pamela DenBesten, R.W. Blacher, Y. Kumagai, W. Li, and He Liu
- Subjects
0301 basic medicine ,Ubiquitin-Protein Ligases ,Blotting, Western ,SUMO-1 Protein ,Down-Regulation ,Biology ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Western blot ,In vivo ,Dental pulp stem cells ,Extracellular ,medicine ,Humans ,Osteonectin ,Amino Acid Sequence ,Receptors, Immunologic ,General Dentistry ,Cells, Cultured ,Cyclin-Dependent Kinase Inhibitor p16 ,Dental Pulp ,Glycoproteins ,Glycosaminoglycans ,Oligonucleotide Array Sequence Analysis ,Extracellular Matrix Proteins ,medicine.diagnostic_test ,Cell growth ,Stem Cells ,Cell Differentiation ,030206 dentistry ,Phosphoproteins ,Molecular biology ,Cyclin-Dependent Kinases ,Peptide Fragments ,Fibronectins ,Up-Regulation ,030104 developmental biology ,Bromodeoxyuridine ,MEPE ,Pulp (tooth) ,Stem cell ,Oligopeptides ,Cell Division - Abstract
Matrix extracellular phosphoglycoprotein (MEPE) is a SIBLING protein, found in bone and dental tissues. The purpose of this study was to determine whether a 23-amino-acid peptide derived from MEPE (Dentonin or AC-100) could stimulate dental pulp stem cell (DPSC) proliferation and/or differentiation. DPSCs were isolated from erupted human molars, and the mitogenic potential of Dentonin in DPSCs was measured by BrdU immunoassay and cell-cycle gene SuperArray. Differentiation of DPSCs with Dentonin was characterized by Western blot and by osteogenesis gene SuperArray. Dentonin enhanced DPSC proliferation by down-regulating P16, accompanied by up-regulation of ubiquitin protein ligase E3A and human ubiquitin-related protein SUMO-1. Enhanced cell proliferation required intact RGD and SGDG motifs in the peptide. This study shows that Dentonin can promote DPSC proliferation, with a potential role in pulp repair. Further studies are required to determine the usefulness of this material in vivo.
- Published
- 2004