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Amelogenin exons 8 and 9 encoded peptide enhances leucine rich amelogenin peptide mediated dental pulp repair
- Source :
- Huang, Y; Goldberg, M; Le, T; Qiang, R; Warner, D; Witkowska, HE; et al.(2012). Amelogenin exons 8 and 9 encoded peptide enhances leucine rich amelogenin peptide mediated dental pulp repair. Cells Tissues Organs, 196(2), 151-160. doi: 10.1159/000331248. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/1x0259mp, Cells, tissues, organs, vol 196, iss 2
- Publication Year :
- 2012
- Publisher :
- eScholarship, University of California, 2012.
-
Abstract
- Amelogenins containing exons 8 and 9 are alternatively spliced variants of amelogenin. Some amelogenin spliced variants have been found to promote pulp regeneration following pulp exposure. The function of the amelogenin spliced variants with the exons 8 and 9 remains unknown. In this study, we synthesized recombinant leucine rich amelogenin peptide (LRAP, A-4), LRAP plus exons 8 and 9 peptide (LRAP 8, 9) or exons 8 and 9 peptide (P89), to determine their effects on odontoblasts. In vivo analyses were completed following the insertion of agarose beads containing LRAP or LRAP 8, 9 into exposed cavity preparations of rat molars. After 8, 15 or 30 days' exposure, the pulp tissues were analyzed for changes in histomorphometry and cell proliferation by PCNA stainings. In vitro analyses included the effects of the addition of the recombinant proteins or peptide on cell proliferation, differentiation and adhesion of postnatal human dental pulp cells (DPCs). These studies showed that in vivo LRAP 8, 9 enhanced the reparative dentin formation as compared to LRAP. In vitro LRAP 8, 9 promoted DPC proliferation and differentiation to a greater extent than LRAP. These data suggest that amelogenin exons 8 and 9 may be useful in amelogenin-mediated pulp repair. © 2012 S. Karger AG, Basel.
- Subjects :
- Male
Histology
Medical Physiology
Peptide
Dental pulp capping
Cell Growth Processes
Amelogenin exons 8 and 9
Article
law.invention
Rats, Sprague-Dawley
Exon
Dental Enamel Proteins
stomatognathic system
law
Animals
Humans
Dental/Oral and Craniofacial Disease
Dental Pulp
chemistry.chemical_classification
Amelogenin
Odontoblasts
Animal
Alternative splicing
Exons
Molecular biology
In vitro
Rats
Dental pulp cells
Disease Models, Animal
Odontoblast
chemistry
Disease Models
Recombinant DNA
Pulp (tooth)
Sprague-Dawley
Biochemistry and Cell Biology
Anatomy
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Huang, Y; Goldberg, M; Le, T; Qiang, R; Warner, D; Witkowska, HE; et al.(2012). Amelogenin exons 8 and 9 encoded peptide enhances leucine rich amelogenin peptide mediated dental pulp repair. Cells Tissues Organs, 196(2), 151-160. doi: 10.1159/000331248. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/1x0259mp, Cells, tissues, organs, vol 196, iss 2
- Accession number :
- edsair.doi.dedup.....215cec057ab382216c3ba98eefab1c09