51. Development of a label-free electrochemical aptasensor based on diazonium electrodeposition: Application to cadmium detection in water.
- Author
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Rabai S, Benounis M, Catanante G, Baraket A, Errachid A, Jaffrezic Renault N, Marty JL, and Rhouati A
- Subjects
- Biosensing Techniques methods, Cations analysis, Diazonium Compounds chemistry, Dielectric Spectroscopy, Electrodes, Electroplating methods, Gold chemistry, Limit of Detection, Reproducibility of Results, Aptamers, Nucleotide chemistry, Biosensing Techniques instrumentation, Cadmium analysis, Electrochemical Techniques methods, Water analysis
- Abstract
In this study we have developed a new aptasensor for cadmium (Cd
2+ ) detection in water. Gold electrode surface has been chemically modified by electrochemical reduction of diazonium salt (CMA) with carboxylic acid outward from the surface. This was used for amino-modified cadmium aptamer immobilization through carbodiimide reaction. Chemical surface modification was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This latter was also used for Cd2+ detection. The aptasensor has exhibited a good linear relationship between the logarithm of the Cd2+ concentration and the impedance changes in the range from 10-3 to 10-9 M with a correlation R2 of 0.9954. A high sensitivity was obtained with a low limit of detection (LOD) of 2.75*10-10 M. Moreover, the developed aptasensor showed a high selectivity towards Cd2+ when compared to other interferences such as Hg2+ , Pb2+ and Zn2+ . The developed aptasensor presents a simple and sensitive approach for Cd2+ detection in aqueous solutions with application for trace Cd2+ detection in spring water samples., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2021
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