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In recent years, CHO（Chinese hamster ovary）cell engineering transformation mainly changes a single function of cells by knock-in or knock-out genes, but the knock-in and knock-out genes often cannot simultaneously play the corresponding functions in a single experimental operation, which limits the application of multigene synchronous transformation. In this study, Bcl-2, the anti-apoptotic gene in the apoptosis pathway, and FUT8, the key enzyme gene of fucose synthesis in the protein fucosylation pathway, were selected as the knock-in and knock-out genes. CRISPR/Cas9 technology was used to establish the site-specific synchronous knock-in and knock-out gene editing strategy. The monoclonal cell line obtained by using this strategy overexpressed Bcl-2 protein and lost FUT8 protease function. After subculture, it was found that the gene expressions of the cell lines obtained by the established site-specific synchronous knock-in and knock-out gene editing strategy were stable within 60 d. Compared with the original wild-type cells, the cell line demonstrated higher tolerance to serum deprivation and stronger resistance to death. This reveals that the gene editing strategy based on site-specific synchronous knock-in and knock-out is feasible and can be used to construct CHO engineering cell lines for recombinant protein production. [ABSTRACT FROM AUTHOR]

目的以上海市疾病预防控制中心承担项目为例,对上海市公共卫生重点学科建设与人才培养的实践进行 容观评估, 总结项目实施经验, 完善项目评估机制, 强化学科发•展与人才培养对上海市健全公共卫生应急管理体 系的支撑作用。方法 基于学科建设验收评估申请书, 通过定量、定性方法对学科建设期间的项目基本情况、学科 建设成效、跨机构科研协作、参与公共卫生实践以及人才培养与建设等进行描述和分析。结果各学科团队承扭的 科研项目级别较高, 较好促进了学科创新发展;各学科团队注重公共卫生实践与应用能力建设, 实现了科研成果 的转化落地;上海市疾病预防控制中心和复旦大学在论文发表合作网络中占据了主要地位, 在多部门科研协作 中发挥了重要作用;专业机构和高等院校的合作促进了公共卫生复合型人才培养。结论上海■市公共卫生重点学 科龜设与人才培养取得了良好成效. [ABSTRACT FROM AUTHOR]

Objective To establish a quality control system of volatile oil of Chunyang Zhengqi capsules. Methods The chromatogram of volatile oil was established by GC method, and the contents of cinnamaldehyde and eugenol were determined. Results In 15 batches of samples, 19 common peaks were identified, and 9 characteristic peaks were selected to establish the characteristic spectrum. The linear ranges of cinnamaldehyde and eugenol were 0.522 - 1.565 mg/ml (r=0.9994) and 3.038 - 9.115 mg/ml (r=0.9997), respectively. The average recoveries were 97.1% and 97.3%, with RSD of 1.5% and 1.4%, respectively. Conclusion The established GC characteristic map and content determination method could control the quality of essential oil in Chunyang Zhengqi capsules qualitatively and quantitatively. The method is accurate and feasible which could be used as the quality control method of essential oil in Chunyang Zhengqi capsules. [ABSTRACT FROM AUTHOR]

Objective To understand the recent infection status and influencing factors of HIV-1 infected men who have sex with men （MSM） newly reported by non-governmental organizations （NGO） in Kunming city from January 2018 to December 2020, and to provide guidance for AIDS prevention and treatment for this population. Methods The limiting-antigen avidity enzyme immunoassay （LAg-Avidity EIA） combined with CD4+ T lymphocyte count was used to determine the recent infections among HIV-1 antibody positive MSM in Kunming city from January 2018 to December 2020. The influencing factors of recent infections were analyzed by Logistic regression. Results A total of 389 HIV-1 antibody positive samples were collected, and the proportion of HIV-1 recent infections was 48.1% （90/187）, 51.6% （49/95） and 30.8% （33/107） from January 2018 to December 2020. There was no statistical difference in the proportion of recent infections between 2018 and 2019 （P > 0.05） . The proportion of recent infections in 2020 was lower than those in 2018 and 2019 （P < 0.05）. Multivariate Logistic regression analysis showed that students （P = 0.017, OR = 2.989, 95%CI: 1.221～7.318） had a high proportion of recent infections. Conclusion The proportion of HIV-1 recent infection among MSM in Kunming showed a decreasing trend, and the proportion of recent infection was relatively higher in the certain population. The targeted measures should be taken, including strengthening the monitoring of recent infections among MSM, improving access to intervention services and promoting HIV testing through NGO. [ABSTRACT FROM AUTHOR]

A rapid screening method for multi-residue of 180 pesticides in Fructus Citri was established by QuEChERS combined with ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS). An accurate mass database and a high-resolution fragment ion spectrum library were established by optimizing the sample preparation and purification, as well as the search parameters such as the accurate mass extraction window, the retention time window and the absolute peak filter. Moreover, the qualitative and quantitative results of the proposed screening method were verified. The results showed that the linear relationship of the 180 pesticides in Fructus Citri was good within the mass concentration range of 0.005-1 mg/L (r > 0.99), and the screening detection limits (SDLs) of 96.6% of the pesticides were below 0.05 mg/kg. The average recoveries at the three spiked levels (0.02, 0.05, 0.1 mg/kg) were 58%-129%, 68%-131% and 66%- 132%, respectively, and the relative standard deviation (RSD) was 0.7%-18% (n=5). Using TOF-MS first-level accurate mass-to-charge ratio screening combined with the confirmation of high-resolution fragment mass spectrometry, the proposed method significantly improved the accuracy of rapid screening for multiple pesticide residues, efficiently reduced the false positive rate, and has universal applicability in the daily monitoring of pesticide residues in Chinese medicinal materials of Fructus Citri. [ABSTRACT FROM AUTHOR]

Objective·To quantitatively evaluate the injury degree and prognosis of inferior alveolar nerve injury in patients with lower lip numbness caused by inferior alveolar nerve injury by using current perception threshold (CPT), electrical pulp test (EPT) and two-point discrimination (2PD), establish a diagnosis and detection scheme for inferior alveolar nerve injury and provide reference for prognosis evaluation. Methods·We selected patients with unilateral inferior alveolar nerve (IAN) injury for more than one month caused by mandibular third molar extraction who went to the outpatient department of oral surgery of the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from July 2018 to January 2021. All patients were followed up regularly, and the visual analog scale (VAS) was used to determine the degree of numbness recovery. During the follow-up, if the patient's VAS<3, it was determined that the patients recovered from numbness and were included in the recovery group. The followup ended. If the follow-up time was more than 1 year, and the patients' VAS≥3, it was judged that the patients' numbness didn't recover and they were included in the unrecovered group. We performed current perception threshold (CPT), electrical pulp test (EPT) and two-point discrimination (2PD) on patients of different groups, and compared the values of the three tests on the healthy side and the correlation with prognosis. Results·A total of 60 patients were included in this study, including 14 males and 46 females. At the end of follow-up, 44 patients had VAS<3, and were setted as recovery group. The average recovery time was (4.1± 1.4) months. The remaining 16 patients did not have VAS<3, and were setted as the non-recovery group. The differences of two sides of all patients' CPT, EPT and 2PD were statistically significant (all P<0.05). For the data of affected side of patients with and without lower lip sensation recovery, only CPT 5 Hz and EPT were significantly different (both P=0.000). CPT (5 Hz) had high sensitivity (93.8%) and specificity (87.5%) in the evaluation of prognosis. Similarly, EPT also had high sensitivity (93.8%) and specificity (93.8%). The CPT (5 Hz) of the affected side was consistent with the value of EPT of the posterior teeth. Conclusion· CPT, EPT and 2PD can evaluate the injury of inferior alveolar nerve. The value measured at CPT 5 Hz and posterior tooth EPT can evaluate the prognosis of nerve recovery. [ABSTRACT FROM AUTHOR]

Formaldehyde (HCHO) is one of the main pollutants in indoor air, which seriously affects the quality of indoor air and endangers human health. Catalytic oxidation at room temperature is an effective method to remove HCHO, which can completely convert HCHO into C()2 and H2 () at room temperature. However, noble metal catalysts are the main catalysts at present, which are expensive and not conducive to large-scale production and application. Tn this paper, Ti()2 carrier is prepared by sol-gel method, and MnFeO工 is supported by impregnation, evaporation, drying and roasting with manganese nitrate and ferric nitrate as active precursors. Meanwhile, the effects of metal oxide loading, molar ratio, roasting temperature and other factors on the catalytic oxidation performance of catalysts at room temperature are investigated. BET, SEM, XRD, H-TPR, ()2-TPT), in-situ DRIFTS and other techniques are used to characterize and analyze the catalysts. The experimental results show that when MnFeO 工 loading is 1() wt%, n (Mn):, z(Fe)=4:l and calcination temperature is 35() °C, the catalyst exhibits the best catalytic oxidation activity at room temperature, and the removal rate of formaldehyde reaches 87.6 % within 48 h. [ABSTRACT FROM AUTHOR]

This paper points out, based on Paul Pelliot's original text, that there are some errors in Feng cheng-chun's Chinese translation. As for the Chinese transcription "吐蕃" is concerned, some analyses are made from the perspective of the bilabial and labio-dental differentiation. The data of fanqie and Sino-Xenic transcription determine the happening of the bilabial and labio-dental differentiation in the beginning of Dynasty Tang; therefore, the Chinese transcription "吐蕃" might as well be far from the ethnonym "bod" in Tibetan. [ABSTRACT FROM AUTHOR]

目的：探讨米力农对老年心力衰竭的辅助治疗作用。方法选择老年心力衰竭患者104例，随机分为观察组和对照组各52例。两组均给予心力衰竭常规治疗，观察组在常规治疗的基础上给予米力农10 mg＋5％葡萄糖注射液250 mL以0．4～0．6μg／（kg· min）泵注给药，1次／d，连续应用3 d。分别于治疗前及治疗后30天采用ELISA法检测两组血浆基质金属蛋白酶9（MMP-9）、B型钠尿肽（BNP）及血清TNF-α和IL-6；行心脏超声检查，记录左心室射血分数（LVEF）、左心室舒张末期内径（LVESD）。结果与治疗前比较，两组治疗后血浆MMP-9、BNP及血清TNF-α、IL-6水平均下降，LVEF升高、LVESD减小（P均＜0．05）；观察组较对照组变化更明显（P均＜0．05）。结论在老年心力衰竭患者治疗中辅助应用米力农可改善心室结构，提高心室射血分数，降低体内炎症反应。 [ABSTRACT FROM AUTHOR]

The frequency data plays more important role in VaR estimation. This paper presents an M-estimator of a proxy periodic GARCH (p,q) scaling model, and gives corresponding estimation method of VaR. Simultaneously we apply it to forecast out-of-time VaR with 5-min high frequency data of CSI 300 Index and HSI. The empirical analysis shows that the estimation method of VaR based on M-estimator with high-frequency data performs better than the results without the use of high-frequency data and QMLE with high-frequency data. [ABSTRACT FROM AUTHOR]

‘Guiduoku 75' bred by distant hybridization was selected to investigate the major agronomic traits, yield traits and quality traits in ratooning plants under the 40 cm(I), 20 cm(Ⅱ)and 10 cm(Ⅲ)of stubble height treatment. The results indicated that the plant height, shoot weight, grain number per plant, yield per plant and yield in ratooning plants were significantly altered by stubble height when stubble height was less than 40 cm. The values of plant height, shoot weight, grain number per plant, yield per plant and yield in ratooning plants under treatment I were significant or extremely significant higher than those of treatments Ⅱ and Ⅲ, suggesting that higher stubble height is beneficial for vegetative organ growth and yield formation in ratooning plants. ‘Guiduoku 75' seeds contained four protein components as albumine content > gluten content > prolamine content > globulin content. The total protein content and gluten content were significantly altered by stubble height, but the albumin content, prolamine content, globulin content were not when stubble height was less than 40 cm. The total flavonoid content in ratooning plants of ‘Guiduoku 75' ranged from 3.17% to 3.33%, that was little affected by stubble height. The ratooning plants of ‘Guiduoku 75' under 40 cm of stubble height treatment harvested 1.5 t·hm-2 seeds that contained 4.06% of albumin content, 3.26% of gluten content, 1.27% of prolamine content, 0.39% of globulin content and 3.33% of total flavonoid content, exhibiting high nutritional value and medicinal value. [ABSTRACT FROM AUTHOR]

Objective To investigate the application prospect of the most extensive genome engineering pig internationally in preclinical xenotransplantation. Methods Porcine endogenous retrovirus (PERV) knockout combined with 3 major heterologous antigen gene knockouts and 9 humanized genes for inhibition of complement activation, regulation of coagulation disorders, anti-inflammatory and anti-phagocytosis were transferred into a pig (PERV-KO/3-KO/ 9-TG) as a donor, and the heart, liver and kidney were obtained and transplanted to 3 Rhesus macaque recipients respectively to establish a preclinical research model of pig-to-Rhesus macaque xenotransplantation. The functional status of xenografts after blood flow reconstruction was observed and the survival of recipients was summarized. The hemodynamics of xenografts were monitored. The change of hematological indexes of each recipient was compared. The histopathological manifestation of xenografts was observed. Results After the blood flow was reconstructed, all xenografts showed ruddy color, soft texture and good perfusion. The transplant heart, liver and kidney showed full arterial and venous blood flow and good perfusion at 1 d after operation. The postoperative survival time of heart, liver, and kidney transplant recipients was 7, 26, and 1 d, respectively. The levels of creatine kinase, creatine kinase isoenzyme, and lactate dehydrogenase increased in heart transplant recipient at 1 d after operation, and gradually recovered to near normal levels at 6 d after operation. All indexes increased sharply at 7 d after operation. The level of aspartate aminotransferase increased in liver transplant recipients at 2 d after operation, and the alanine aminotransferase basically returned to normal at 10 d after operation, but the total bilirubin continued to increase. Both aspartate aminotransferase and alanine aminotransferase increased at 12 d after operation, and reached a peak at 15 d after operation. The kidney transplant recipient developed mild proteinuria at 1 d after operation, and died of sudden severe arrhythmia. Histopathology showed that the tissue structure of cardiac and renal xenografts was close to normal, and liver xenografts presented with patchy necrosis, the liver tissue structure was disordered, accompanied by inflammatory damage, interstitial hemorrhage and thrombotic microangiopathy. Conclusions PERV-KO/3-KO/9-TG pig shows advantages in overcoming hyperacute rejection, mitigating humoral rejection and coagulation dysregulation. However, whether it can be used as potential donor for clinical xenotransplantation needs further evaluation. [ABSTRACT FROM AUTHOR]

Objective To the investigate the safety and efficacy of reverse use tirofiban and contrast agent through thrombus suction catheter after occlusion artery route in hyperthrombotic load patients with acute myocardial infarction. Methods From July 2018 to October 2019, 376 acute STEMI patients treated by emergency percutaus coronary intervention were admitted to the hospital successively. According to the different treatment methods，the 376 acute STEMI patients were divided into the study group (n = 54) and the control group (n = 322). The effects of two different routes of administration on postoperative myocardial perfusion (TIMI flow grades) and the incidence of severe hemorrhage，stroke and serious cardiac adverse events during hospitalization were compared. Results The preoperative thrombotic load of the study group was significantly higher than that of the control group (3.23±0.95 vs 2.14±0.66，P < 0.05). There was no significant difference in the incidence of intraoperative complications (P > 0.05)，and there was no significant difference in the postoperative rate of TIMI 3 between the two groups （95.3% vs 96.3，P > 0.05). There was no significant infference to the incidence of severe hemorrhage，stroke during PCI and serious cardiac adverse events during the hospitalization between the two groups（P > 0.05). Conclusion With the use of tirofiban and contrast agent through thrombus suction catheter after the occlude artery route，it can effectively improve the myocardial perfusion and reduce the perioperative risk of acute STEMI patients with hyperthrombotic load and it is worthy of further study. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical effect and safety of transcatheter arterial chemoembolization (TACE) combined with microwave ablation (MWA) in the treatment of advanced primary liver cancer. Methods A total of 186 patients with advanced primary liver cancer who were treated in The Fifth Medical Center of Chinese PLA General Hospital from April 2015 to June 2019 were enrolled and divided into study group and control group using a random number table, with 93 patients in each group. Both groups of patients underwent TACE, and the patients in the study group were treated with ultrasound - guided percutaneous MWA. The two groups were compared in terms of clinical outcome and complications. Quantitative real - time PCR was used to measure the serum level of microRNA - 202 (miR - 202), ELISA was used to measure the serum levels of fragile histidine triad (FHIT) and P16 protein, and the changes in the above three indices at 3 months after treatment were compared. The two - independent - samples t test was used for comparison of continuous data between two groups, and the paired t - test was used for comparison within one group before and after treatment; The chi - square test was used for comparison of categorical data between groups. Results The study group had a significantly higher objective response rate than the control group (47.32% vs 27.96%, x² = 7.422, P = 0. 006), and there was no significant difference in disease control rate between the two groups( P > 0. 05) . Both groups had significant increases in the serum levels of miR - 202, FHIT, and P16 protein at 3 months after treatment (all P < 0.05), and compared with the control group, the study group had significantly higher serum levels of miR - 202 (0.84 ± 0.14 vs 0.58 ± 0.17, t = 11.385, P < 0.001), FHIT (1126.35 ± 73.05 pg/ml vs 762.87 ± 56.71 pg/ml, t = 37. 904, P < 0.001), and P16 protein (52. 86 ± 6.51 pg/ml vs 39.06 ± 5.37 pg/ml, t = 15.770, P < 0.001). Conclusion Ultrasound - guided MWA in addition to TACE can improve the short - term response of patients with advanced primary liver cancer and increase the serum levels of miR - 202, FHIT, and P16 protein, with relatively high safety. [ABSTRACT FROM AUTHOR]

Poilution of indoor volatile organic compounds (VOCs), such as formaldehyde and benzene, has be come one of the major environmental problems for the broad masses of the people in our daily life and produc tion. The major methods used to purify VOCs in the room are as follows: adsorption, phorncatalytic oxidation, low temperature plasma and catalytic oxidat ion. In particular, catalytic oxidation at ambient temperature has got more attention due to the advantages of high purification efficiency and stability, no secondary pollution, and wide availability. Therefore, MnCe0x was used as the active component, and palygorskite and kaolin with a lot of pores were used as supporters to develop MnCe0x/palygorskite and MnCeox-palygorskite catalysts in this work. Meanwhile, the effects of loading amount, preparation, composition and structure on the catalytic oxida tion of formaldehyde at room temperature were investigated. It was found that the oxidation activity for the supported MnCeOx/palygorskite catalyst increased with the increasing of the loading amount, and the best method was hybrid method with solutions. In addition, the oxidation activity depended on the types of mixing components for the integrated MnCeox-palygorskite catalysts. Among them, attapulgite or aluminum hydroxide showed preferable performance for catalytic oxidation of formaldehyde at room temperature. [ABSTRACT FROM AUTHOR]

该文对3例疑似新型冠状病毒肺炎(简称新冠肺炎)危重患者早期救治的护理进行总结。对2020年2月该院收 治的疑似新冠肺炎危重患者采取了严密隔离、呼吸支持、皮肤护理、营养支持、功能锻炼及心理护理等护理措施。3例疑似新冠肺 炎危重患者在排除新型冠状病毒感染后脱离生命危险,顺利平稳地转入住院部继续治疗。通过实施严密隔离、呼吸支持、皮肤护 理、营养支持、功能锻炼、心理护理等干预措施是救治疑似新冠肺炎危重患者有效的护理措施. [ABSTRACT FROM AUTHOR]

Objective · To introduce the design and manufacture of the custom-made temporomandibular joint (TMJ)-mandible prosthesis and evaluate its safety, effectiveness and accuracy in clinical application. Methods · Six patients with the TMJ osteoarthrosis were included in this study. The maxillofacial CT data for all the patients were obtained and transferred into the Mimics 18.0 software preoperatively. The custom-made TMJ-mandible prosthesis was designed based on the anatomy and movement of the TMJ, and fabricated by 3D printing and 5-axis milling technologies. The TMJmandible lesion has been removed completely from modified preauricular, post and sub mandibular incisions. The combined prosthesis has been implanted after complete lesion resection. Then, the iliac or fibular bone has been grafted and fixed in the medial side of the prosthesis. The clinical follow-up and radiography examinations were taken after surgery to assess the safety and effectiveness for using. The postoperative CT scan was used to measure the implanted deviation compared with the preoperative design. Results · During the operations, all prosthesis were positioned smoothly, easily adapted to the bone surfaces, and fixed excellently. There were no prosthesis displacement, loosening, and fracture in X-ray postoperatively. The pain, diet, mandibular movement and open mouth had significant improvements after 19 months' follow-up. The merged postoperative CT scans indicated that the mean differences between predesigned positions of prosthesis and postoperative implantation sites were (0.891±0.127) mm for the fossa component and (1.240±0.246) mm for the mandibular component. The total average error was (1.122±0.198) mm. Conclusion · The TMJ-mandible combined prosthesis is safe, effective, and precise for the clinical application. [ABSTRACT FROM AUTHOR]

德谷胰岛素是一种新的基础胰岛素类似物,通过去掉人胰岛素B30位苏氨酸,在B29位赖氨酸由一个L-r-谷氨酸连接一个16碳脂肪二酸侧链,皮下注射后因苯酚的快速弥散和脂肪二酸侧链的自我聚集形成独特的多六聚体长链,在注射部位形成储存库减慢其吸收,并在锌离子的缓慢弥散下以单体的形式入血,再与白蛋白结合到达靶器官从而发挥作用;其半衰期约25 h,作用时间超过40 h,在给药后24 h各时段血药浓度分布均匀,药代动力学曲线平稳,各年龄阶段的药代动力学曲线形状相似,肝肾功能受损患者的药代动力学特点与正常人无显著差异,给药间隔时间在8～40 h内有效性和安全性不受影响,注射时间更灵活。与其他基础胰岛素相比,德谷胰岛素在1型糖尿病、2型糖尿病及糖尿病合并心血管疾病的患者中均能有效降糖,低血糖(包括夜间低血糖)风险低,体质量增加无显著差异;但在合并肾损伤患者中的疗效及低血糖风险结论不一,有研究发现肾小球滤过率(eGFR)<60 mL/(min·1.73 m~2)的糖尿病患者德谷胰岛素的疗效不及甘精胰岛素,随着患者eGFR的下降,德谷胰岛素的低血糖及严重低血糖风险可能增加;德谷胰岛素在糖尿病合并妊娠患者中的应用效果与甘精胰岛素相似。 [ABSTRACT FROM AUTHOR]

Objective To explore the learning curve of fetal system ultrasound examination in middle pregnancy for continuing education physicians. Methods From January 2018 to December 2018， 6 trainee physicians were selected to learn fetal system ultrasound examination during mid-trimester in our department. According to their work experience， they were divided into group A （ultrasound work in gynecology and obstetrics for ≥ 2 years） and group B （ultrasound work for ＜ 2 years）， with 3 physicians in each group. The first 200 cases of ultrasound examination data of each physician were selected， a total of 1 200 cases were analyzed. According to the time sequence of ultrasound examination， each 40 cases was a learning curve segment，and there were 5 learning curve segments. Each case was scored according to the standard ultrasonographic images. The average image scores between each stage and between groups A and B were compared， and the learning curve was drawn. Results The average scores of the two groups showed an upward trend. There was significant difference in the average scores of each stage in each group （ < 0.05） . There was significant difference （ < 0.05） between Group A and Group B at Stage 1～4 ， but no significant difference at stage 5 （ > 0.05） . The inflection point of operation score was 120 cases in Group A and 160 cases in Group B. Conclusion It is a gradual process to learn fetal system ultrasound examination during mid-trimester. Trainee physicians with basic ultrasound operation in gynecology and obstetrics need to complete about 120 cases， and those without basic ultrasound operation in gynecology and obstetrics or with little experience need to complete about 160 cases，then they can master the skills of fetal system ultrasound examination in the second trimester. [ABSTRACT FROM AUTHOR]

To evaluate the role of bacterial infection related inflammatory biomarkers in identifying multi- drug resistant (MDR) bloodstream infections (BSIs) confirmed by blood culture (BC) and predicting pathogen types. Methods A retrospective analysis was conducted on 852 MDR infection patients in the Third People’s Hospital of Hubei Province, while 526 patients with concurrent laboratory test results including blood culture, routine blood tests, C reaction protein (CRP), procalcitonin (PCT) and serum pancreatic amyloid (SAA). The differences of the above indicators were analyzed in negative blood culture and positive blood culture groups, and the diagnostic value for the bloodstream infections was also evaluated. Results In 526 patients, there were 108 BSIs cases, including 42 cases (38.9%) were infected with Gram-positive bacteria and 66 cases (61.1%) were infected with Gran-negative bacteria. MRSA (23 cases), ESBL-positive Escherichia coli (39 cases) and ESBL-positive klebsiella pneumoniae (20 cases) were the major pathogens in BSIs. In BCpositive cases, there were significant differences in CRP, PCT, SAA and NEU between Gram-negative bacteria group and Gram-positive bacteria group (Z=2.448, 5.647, 3.368, 4.905, all P＜0.05). Within cases of Gram-positive bacteria, there was statistical significance in CRP (H=19.021, P＜0.001), with the highest level of MRSA (114.35 mg/L). Within cases of Gram-negative bacteria, both CRP and PCT showed statistical significance (H=19.369, 15.013, P＜0.01), with the highest level in ESBL-positive escherichia coli (164.60 mg/L, 97.42-217.50; 7.361 μg/L, 3.51-9.95). The area under the receiver operating characteristic curves (ROC-AUCs) of CRP, PCT, SAA, WBC and NEU for discriminating positive MDR-BC from MDR-BC negative cases were 0.778, 0.728, 0.658, 0.578, and 0.645, with statistical significance in CRP and PCT (P＜ 0.01). For discriminating Gram-negative bacteria BC, AUCs of CRP and PCT were 0.692 and 0.883 with statistical significance (P＜0.01). Conclusion CRP is more applicable for the early diagnosis of BSI compared with PCT. After confirming BSI, PCT is suitable for discriminating Gram-negative bacteria infection. Therefore, the combination of PCT and CRP may help medical staff to judge the BSI and type the possible pathogens, and to rationally use drugs. [ABSTRACT FROM AUTHOR]

Objective To investigate the validation of fracture risk assessment tool FRAX® in identifying osteoporotic vertebral fracture (OVF) in community-dwelling elderly people in Shanghai. Methods Stratified sampling was performed according to the age distribution of the population in communities in Shanghai.Totally 2809 elderly aged over 60 years from 5 communities were enrolled.Questionnaires and clinical physical examination (height and weight) were recorded. The estimated 10-year absolute risks of major osteoporotic fractures (MOF) were calculated with the FRAX® algorithms for China. OVF were ascertained from X-radiographs of thoracic and lumbar spine.The results were assessed according to the semi-quantitative technique of Genant. Results In females,age,proportion of previous fracture history and FRAX®MOF value of OVF group were higher than that of non-OVF group (P<0.001).In males,the proportion of previous fracture history,alcohol intake,rheumatoid arthritis and FRAX®MOF value of OVF group were higher than that of non-fracture group (P<0.05 or P<0.01).The prevalence of OVF increased with the rising of FRAX®MOF value according to the quartile groups (Q1 to Q4) in females (P<0.001).The prevalence of OVF increased with the rising of FRAX®MOF value according to the quartile groups (Q1 to Q3)and declined in Q4 in males (P=0.035).The sensitivity and specificity for FRAX®MOF to identify OVF was 81.9% and 53.9%,to identify moderate-severe OVF was 91.7% and 56.8%,and to identify multiple OVF was 64.7% and 65.5% in females (P<0.001);the sensitivity and specificity for FRAX®MOF to identify OVF was 67.8% and 45.9% (P<0.001),and to identify moderate-severe OVF was 69.2% and 53.6% in males (P=0.003).Conclusions FRAX® may be a simple and effective tool for identifying the risk of OVF,especially for moderate-severe OVF in community-dwelling elderly women. [ABSTRACT FROM AUTHOR]

Objective To investigate the indications, safety and effectiveness of fascia-pedicled perforator flap for repairing tissue defects after orbital enucleation. Methods From September 2016 to Decmber 2020, according to the anatomical distribution of the famous periorbital artery, 16 patients (16 eyes) were selected to repair the tissue defect after orbital content enucleation with island flap formed by the famous artery as fascia pedicled and transferred to the defect area through subcutaneous tunnel. The defect area was 3.0 cm×3.0 cm~ 14.0 cm×14.0 cm, the flap area was 4.0 cm×4.0 cm~15.0 cm×15.0 cm. The survival and appearance of the flap were observed after 3 to 6 months follow-up. Results The skin flaps survived well. The skin flaps had soft texture and good sensory function. Among them, 2 cases of perforating branch skin flap needed second stage operation to cut the pedicle, and 1 case had venous reflux disorder in early postoperative stage. After active treatment, the skin flap survived well. One case underwent full-thickness free skin grafting 1 month after operation due to epidermal necrosis due to venous stasis. Conclusion Subcutaneous pedicle perforators flap with blood supply, overcome the cat ear tissue thickness moderate texture is more advantageous to improve the appearance, flap late radiation and chemotherapy of the advantages of early intervention, gouge is repairing orbital contents in addition to good skin flap of large peri-orbit tissue defect after surgery. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of acupoint pressing and application based on midnight-noon ebb-flow theory on sleep quality of patients receiving dexamethasone pretreatment before paclitaxel. Methods Totally 88 patients who received dexamethasone pretreatment before paclitaxel were randomly divided into the observation group and control group, with 44 cases in each group. Patients in the control group received routine nursing and acupoint pressing and application, and those in the observation group were given routine nursing and acupoint pressing and application based on midnight-noon ebb-flow theory. The applied interventions lasted 1 week. The sleep quality and quality of life were measured using Pittsburgh Sleep Quality Index (PSQI) and WHO quality of life assessment instrument (WHOQOL-Bref) respectively. The intervention efficacy was measured and compared between two groups. Results Patients in the observation group achieved a better PSQI compared with patients in the control group after intervention(P＜0. 05). The score of WHOQOL-BREF physiological domain in the observation group was higher than that in the control group at first day and 8th day of intervention(P＜0. 05). There was no significant difference in overall score, psychological, social or environmental score between two groups(P＞0. 05). The overall effective rate in the observation group was higher than that in the control group (81. 81% vs. 74. 45%, P＜0. 05). Conclusion Acupoint pressing and application based on midnight-noon ebb-flow theory is potentially effective to improve the sleep quality and quality of life among patients receiving dexamethasone pretreatment before paclitaxel (目的 探讨子午流注纳支法穴位按压贴敷改善地塞米松预处理紫杉醇化疗患者睡眠质量的效果。方法 选取2022年3月—2023年2月医院肿瘤内科行紫杉醇静脉治疗的88例患者作为研究对象, 随机分为观察组(n=44)和对照组(n=44)。对照组在执行常规护理的基础上, 再进行穴位按压贴敷, 但不择时。观察组在执行常规护理的基础上, 利用子午流注理论进行穴位按压贴敷。干预持续1周。在确定化疗方案当天及第8天分别进行匹兹堡睡眠质量指数(PSQI)评分及世界卫生组织生存质量测定量表简表(WHOQOL-BREF)评分, 评估两组患者干预效果。结果 PSQI量表总分改善观察组优于对照组, 差异有统计学意义(P＜0. 05)。观察组WHOQOL-Bref量表生理领域评分在干预后第1天及第8天均高于对照组(P＜0. 05); WHOQOL-Bref量表总分及其他维度评分两组间差异无统计学意义(P＞0. 05)。观察组总有效率81. 81%(36/44), 高于对照组的70. 45%(31/44), 差异有统计学意义(P＜0. 05)。结论 子午流注纳支法穴位按压贴敷能改善大剂量地塞米松预处理紫杉醇化疗患者的睡眠质量, 从而提高患者的生活质量。)

[Objectives] In present work, the effect of ABA synthetic gene StiNCED2 on potato tuber formation and the possible mechanisms were investigated to explore the potential genes for improving potato tuber yields. [Methods] The expression levels of AHA synthetic genes StNCEDl and StNCED2 were analyzed by semi-quantitative KT-PCK assay to identify the main gene in tuber formation. Transgenic lines overexpressing StNCED2 were constructed and obtained by genetic engineering and transformation. FCK-based molecular identification and ABA content determination verified the positive lines. The potato tubers of selected T1 and '17 lines with control were collected by two-season field planting to analyze the yields. QKT-PCK assays analyzed the expression changes of genes involved in ABA signaling, GA synthesis and metabolism, GA signaling in transgenic lines T1, T7 and control. [Results] StNCED1 expressed in initial stolon and elongated stolon, but not in swollen stolon, initial tuber and mature tuber while StNCED)2 expressed in the whole process of tuber formation, and the highest expression was found in elongated stolon, which was selected for further study. StNCED)2 overexpression lines Tl and '17 had higher expression levels of StNCED)2 and more ABA contents than the control. By planting Tl, '17 and control lines in two seasons, potato yield analysis showed that overexpressing StiNCED2 can promote tuber yield due to the increase of single potato tuber weight, not the tuber number. QKT-PCK assay showed that ABA signaling transcription factor St AH El and GA metabolism gene StGA2oxl were up-regulated while GA synthetic genes StGA3ox2, StGA20oxl and GA signaling transcription factor GAMYH were down-regulated in stolon and tubers of overexpression lines, suggesting there might be a direct interaction between ABA and GA. [Conclusions] ABA synthetic gene StNCED2 can promote tuber formation and is a potential gene for increasing potato yield by genetic engineering.[Objectives] In present work, the effect of ABA synthetic gene Sti\CED2 on potato tuber formation and the possible mechanisms were investigated to explore the potential genes for improving potato tuber yields. [Methods] The expression levels of AHA synthetic genes Sti\CEDl and StSCED2 were analyzed by semi-quantitative KT-PCK assay to identify the main gene in tuber formation. Transgenic lines overexpressing StNCED2 were constructed and obtained by genetic engineering and transformation. FCK-based molecular identification and ABA content determination verified the positive lines. The potato tubers of selected T1 and '17 lines with control were collected by two-season field planting to analyze the yields. QKT-PCK assays analyzed the expression changes of genes involved in ABA signaling, GA synthesis and metabolism, GA signaling in transgenic lines T1, T7 and control. [Results] StNCED1 expressed in initial stolon and elongated stolon, but not in swollen stolon, initial tuber and mature tuber while StNCED)2 expressed in the whole process of tuber formation, and the highest expression was found in elongated stolon, which was selected for further study. StNCED)2 overexpression lines T1 and '17 had higher expression levels of StNCED)2 and more ABA contents than the control. By planting T1, '17 and control lines in two seasons, potato yield analysis showed that overexpressing StNCED2 can promote tuber yield due to the increase of single potato tuber weight, not the tuber number. QKT-PCK assay showed that ABA signaling transcription factor St AH El and GA metabolism gene StGA2oxl were up-regulated while GA synthetic genes StGA3ox2, StGA20oxl and GA signaling transcription factor GAMYH were down-regulated in stolon and tubers of overexpression lines, suggesting there might be a direct interaction between ABA and GA. [Conclusions] ABA synthetic gene StNCED2 can promote tuber formation and is a potential gene for increasing potato yield by genetic engineering. [ABSTRACT FROM AUTHOR]

[Objective] The purpose of this study is to explore the effects of Electron beam to target X-rays on Radiation of Red Spider Lily and Zephyranthes grandiflora. [Method] In this study. Red Spider Lily and Zephyrantlies grandiflora bulbs were irradiated by Electron beam to target X-rays at doses of 25, 50. 75, 100 Gy and 2 MeV 10 Ma respectively,and statistical analysis of germination rate,plant height, leaf number, leal area, root length and root number after irradiation was conducted. [Result] The results showed that different doses of irradiation had the damage effect on the plants, mainly manifested as germination lag, shorter plants, fewer leaves, reduced leal area, shorter root length and reduced root number. There was no significant difference in the growth index between the treatment groups, however, the difference between the treatment group and the control group was large. Red Spider Lily and Zephyranthes grandiflora were inhibited bv X-ray irradiation after electron beam irradiation. [Conclusion] The main purpose of this study was to explore the effects of different doses of Electron beam to target X-rays on the growth and development of Red Spider Lily and Zephyranthes grandiflora, so as to lay the foundation for the successful breeding of new varieties and provide a reference for mutation breeding. [ABSTRACT FROM AUTHOR]

目的了解青岛地区2015年1月～2018年12月出生的473 497例活产新生儿先天性甲状腺功能减低症(CH)发病情况。方法 473 497例活产新生儿,出生72 h之内采集足跟血,时间分辨荧光分析法(TRFIA)检测促甲状腺激素(TSH)。初次筛查TSH值<8μU/m L者为阴性,初次筛查TSH值异常(TSH值≥8μU/m L)者为初筛阳性,初筛阳性者需复检足跟血TSH,两次足跟血TSH值均异常(TSH值≥8μU/m L)者需召回复查,采集静脉血并检测血清TSH和游离甲状腺素(FT4)进行确诊。血清TSH值增高且FT4值降低者诊断为CH,血清TSH值增高、FT4值正常者诊断为高TSH血症。结果 473 497例新生儿中,初筛阳性者5 859例,其中5 385例接受复检,最终确诊CH 234例、高TSH血症39例,CH筛查阳性率0. 49‰。234例确诊患儿中,足跟血初筛TSH值>11μU/m L者201例(占85. 90%),TSH值8～11μU/m L者19例(占12. 39%),5μU/m L [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effect and mechanism of dioximine pretreatment on transplanted donor heart in New Zealand white rabbits. Methods Thirty-six healthy adult New Zealand white rabbits are randomly divided into the control group, dioximine pretreatment group, and dioximine + diethyldithiocarbamate sodium(DDTC) pretreatment group. The rabbits in the control group were fed normally. The rabbits in the dioximine pretreatment group were given dioximine 80 mg/kg by stomach tube, twice a day, 10 days before the experiment. The rabbits in the dioximine +DDTC pretreatment group were given dioximine 80 mg/kg by stomach tube, twice a day, 10 days before the experiment, and DDTC 300 mg/kg was injected into the edges of ears at 15 min before heart transplantation. The myocardial enzyme, apoptosis index and water content in the myocardial tissues were compared among groups, and the myocardial tissues were observed under the microscope. Results Compared with the other two groups, the myocardial enzyme index, myocardial apoptosis index, and the water content of myocardial tissues decreased in the dioximing pretreatment group at 30, 60, and90 min after the donor heart resuscitated(all P<0.05), and the myocardial tissue structure under microscope was better. All the indexes in the dioxomine + DDTC pretreatment group were lower than those in the control group, but the difference was not statistically significant(all P>0.05), and the myocardial structure under microscope was severely damaged. Conclusion Dioximine pretreatment has a protective effect on rabbit transplanted donor heart, and its mechanism is related to the improvement of antioxidant free radical ability. [ABSTRACT FROM AUTHOR]

【Objective】R. omeiensis Rolfe f. omeiensis CBF gene was cloned and analyzed to enrich basic data in rose CBF gene. 【Method】Specific primers were designed. The CDS region and part of 5’UTR and 3’UTR of Ro-DREB1 C were cloned. The physicochemical properties, secondary structure, tertiary structure of protein and prediction protein subcellular localization were analyzed by bioinformatics methods.The phylogenetic tree was constructed to identify gene family of Ro-DREB1 C. 【Result】Ro-DREB1 C, a DREB orthologue, was isolated from R. omeiensis Rolfe f. omeiensis. GenBank accession number was KX397104. The Ro-DREB1 C gene contained a 603 bp length CDS, which encoded a polypeptide of 200 amino acids. The protein molecular formula was C968 H1539N263O299S11 S11 with high ratio of Ser(11.5 %), Leu(11.0 %) and Ala(9.5 %). It was an unstable low-acid and hydrophilic protein. The protein could not be classified as specific secondary structure such as pleated sheet or helical peptide segments, because the secondary structure of Ro-DREB1 C protein was mainly random coil(71.5 %). A‘d1 gcca’template was scanned with Ro-DREB1 C protein sequence using Phyre2 service, query sequence range was 59 to119, and 61 residues had been modeled with 99. 9 % confidence. Ro-DREB1 C protein didn’t have transmembrane structure and signal peptide. Subcellular localization of the protein was mainly in the nucleus. Align c DNA region and amino acid sequence between Ro-DREB1 C and RhCBF showed that twenty one SNPs were found, and eight SNPs were synonymous mutations and the others deduced ten variant amino acids. Integrate phylogenetic tree analysis showed that Ro-DREB1 C was member of the DREB subfamily A-1 of ERF/AP2 transcription factor family. 【Conclusion】R. omeiensis Rolfe f. omeiensis can be preserved and used as an excellent cold tolerant species in roses breeding. [ABSTRACT FROM AUTHOR]

In order to solve the design problem of the bidirectional DC/DC converter applied for the two-way interaction between electric cars and power grids, a full research aiming at the characters of the application, load and the characters of the dual active bridge and resonant CLLC topology was conducted. According to the research about the application environment and the load, a topology achieving soft switching in wide gain range should be chosen for the bidirectional DC/DC converter used for this application. Two topologies, dual active bridge and resonant CLLC, were compared based on the application environment, and the better one, resonant CLLC topology was chosen. Design guideline and key calculation formula were given for the resonant CLLC topology. A 3 k W bidirectional DC/DC prototype based on the resonant CLLC topology applied for V2 G was designed based on the design guideline. Experimental results indicate that the efficiency of this converter based on Si MOSFETs achieved 96. 6% in both forward mode and backward mode, when the switch frequency above 100 k Hz. [ABSTRACT FROM AUTHOR]

Objective: To investigate the acceptance of disability for ischemic stroke patients and its related factors, in order to provide a theoretical basis on rehabilitation intervention in ischemic stroke patients with mental disability. Methods: A total of 180 patients with ischemic stroke were recruited. The Adaptation of Disability Scale-Revised (ADS-R), Medical Outcomes Study Social Support Survey (MOS-SSS), Herth Hope Inder (HHI) and Patient Health Questionnaire-9 (PHQ-9) were used to evaluate the patients. Results: The mean score of acceptance of disability was (88. 2 ± 14. 3) in ischemic patients. Multiple stepwise regressions indicated that the religion, education, MOS-SSS scores and HHI scores were positively associated with ADS-R scores) b'= 0. 33, 0. 21,0. 30, 0. 15). The medical insurance, disability)MRS) and PHQ-9 scores were negatively associated with ADS-R scores(b'= -0. 16, - 1.53, -0.43). Conclusion: The acceptance of disability in patients with ischemic stroke may be at a low level. The religion, education, medical insurance, disability, social support, hope level and depression may be related to the adaptation of disability. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aims to understand the volatile components of R. odorala complex and provide experimental evidences for its full utilization. [ Method] Volatile components in flower petals of R. odorala complex were determined by the methods of head-space solid-phase microextraction (HS-SPME)and gas chromatography-mass spectroscopy (GC-MS). [Result] 56 volatile components were identified from Rosa odorala complex. By volatile components analysis of different varieties, it was found that the main aroma components of R. odorala var odorala were alcohols and tire content of phenylethyl alcohol was tire highest. The main volatile components of R. odoralavar var gigalea and R. odorala var odorala were aromatic hydrocarbons, and their contents of 3,5-Dimethoxytoluene were the highest. The main volatile components of R. odorala var pseuxloindica were aromatic hydrocarbons and terpenes, and tire content of 1,3,5-trimethoxybenzene was the highest. [Conclusion] The volatile components were significantly different among R. odorala complex. Ilie results provided a foundation for understanding different aromatic components in R. odorala complex and cloning significant scented gene and theoretical basis for molecular regular mechanism of scent compounds in roses. [ABSTRACT FROM AUTHOR]

In order to improve teachers' work efficiency and teaching quality in the context of maturing image processing technology and various Internet methods, an online marking system, suitable for university-level teaching and testing, is developed and exemplified by calculus at Donghua University, based on scanning and recognition technology. The system is composed of modules such as test paper scanning, online marking, and statistical analysis. Both sides of test papers are scanned and the consequent images are stored digitally. The objective parts of the papers are graded automatically by the computer while the images of the subjective parts are assigned to and marked by teachers online. All the results are collected electronically into the total grades and analyzed in terms of accuracy rate and difficulty of each part. The monthly tests of calculus shows that the online marking system conforms to the growing trend of digitalization, normalization and modernization of education and its management and assessment. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical value of Histogram Strain tissue dispersion imaging in noninvasive diagnosis of chronic liver injury. Methods: Liver elasticity of 180 patients with chronic hepatitis (105 viral hepatitis and 75 non-viral hepatitis) was quantitatively detected by elastic imaging technique. The correlation between liver elasticity characteristic value and liver fibrosis and inflammation degree were analyzed. Results: The LF diagnostic cut-off value in differentiating mild and moderate to severe fibrosis in 180 chronic hepatitis patients was 2.785, with a sensitivity of 76.6% and specificity 48.9%. The LF diagnostic cut-off value in differentiating mild and moderate to severe inflammation in 180 chronic hepatitis patients was 2.925, with a sensitivity of 73.2% and specificity 56.8%. The LF diagnostic cut-off value in differentiating mild and moderate to severe fibrosis in 105 viral hepatitis patients was 2.975, with a sensitivity of 69.2% and specificity 67.1%. The LF diagnostic cut-off value in differentiating mild and moderate to severe inflammation in 105 viral hepatitis patients was 2.915, with a sensitivity of 80.0% and specificity 57.8%. Conclusion: The LF index was not correlated with degrees of liver fibrosis and inflammation in 75 non-viral hepatitis patients. LF index can reflect the degree of liver fibrosis and inflammation, the higher the diagnostic sensitivity, the greater the LF index, the more severe liver fibrosis and the degree of inflammation. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical effect of alendronate (ALN) combined with alpha ossification in the treatment of in postmenopausal osteoporosis(PMO).Methods:98 cases of PMO patients treated in the outpatient department in our hospital from January 2013 to March 2015 were selected and divided into two groups with the method of random number table.The control group,on the basis of conventional calcium,was given the treatment of alcohol;the observation group,on the basis of the control group,was given ALN treatment.The ward's triangle,femoral neck and lumbar spine L2～L4 bone mineral density (BMD) level,turn,flexion and spontaneous low back pain visual simulation score(VAS),serum bone gla protein(BGP),bone alkaline phosphatase(BALP),type I collagen C telopeptide(CTX) and tartrate resistant acid phosphatase 5b(TRACP-5b) level before and after the treatment,and adverse reactions during medication were recorded and compared between the two groups.Results:In the observation group after the treatment,ward triangle area,the femoral neck and lumbar BMD L2～L4 level compared with those before the treatment,significantly improved (P＜0.05),and the improvement degree of the observation group was more obvious (P＜0.05);as for the patients in the observation group,flexion extension,spontaneous and turning back pain VAS scores compared with those before the treatment significantly decreased (P＜0.01),and the improvement degree of the observation group was more significant (P＜0.01);after the treatment,the serum levels ofBGP,BALP,CTX and TRACP-5b in the observation group significantly decreased compared with those before treatment(P＜0.01),and the improvement degree of the observation group was more significant (P＜0.01).Conclusion:ALN combined with alpha ossification in the treatment of the treatment of PMO can significantly improve the level of BMD,relieve patients' pain symptoms and improve bone metabolism indicators,effectively,safely and reliably,with a high clinical reference value. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy of biyuantang syndrome differentiation plus subtraction in the treatment of chronic sinusitis and its effect on the symptoms and pain of patients. Methods: 117 patients with chronic sinusitis admitted to our hospital outpatient from june, 2016 to june, 2018 were selected and randomly divided into the control group and the observation group. In the control group, 58 cases were treated with conventional western medicine, while 59 cases in the observation group were treated with Biyuantang. The clinical effects, clinical symptoms and improvement of pain were compared between the two groups. Results: After treatment, the effective rate of observation group was 89.83%, which was significantly higher than that of the control group (70.69%, P<0.05), while the symptom score of observation group was significantly lower than that of the control group (P<0.05). In addition, the VAS score of observation group after treatment was (0.74+0.13), which was also significantly lower than that of the control group (1.52+0.30) (P<0.05). Conclusion: Biyuantang can significantly improve the clinical symptoms, reduce the pain feeling and improve the clinical efficacy of patients with chronic sinusitis. [ABSTRACT FROM AUTHOR]

This paper designed a simple method to fabricate Fe3O4 microsphere-graphene oxide (Fe3O4-G0) composite which is applied for the determination of phenolic compound. The Fe3O4 microspheres were well-distributed on the graphene oxide ( GO) surface after ultrasonic treatment. GO was synthesized from natural graphite flakes by the hummers method. Fe3O4 microspheres about 200 nm in diameter were synthesized by the solvothermal method, and scanning electron microscopy ( SEM ) was used to explore the morphology of Fe3O4-G0. This work showed that the approach is simple and effective for the preparation of Fe3O4-G0, which can be effectively applied to detect the phenolic compounds. [ABSTRACT FROM AUTHOR]

ABSTRACT Objective: To investigate the effect of SAHA on the radiosensitization and proliferation of pancreatic cancer cell line Patu8988. Methods: Different concentrations (0, 0.5, 1, 2, 4, 6, 8 µmoL L) of SAHA were treated in human pancreatic cancer cell line Patu8988 for 12. 24. 36 and 48 h. the growth inhibition was detected by MTT colorimetric assay, and the effect of IC50 was calculated. The blank control group and SAHA treatment group (20% IC50 SAHA 24 h) were set and treated by 6MV-X (0, 2, 4, 6, 8Gy) irradiation, clone formation radiosensitizing effect on Patu8988 cells was examined by SAHA assay, multi-target click model fitting cell survival curve, radiation related parameters of DO. Dq the value and radiosensitization ratio were calculated. Western Blot was used to detect the effects of different concentrations (0, 4, 8, 12 µmoL/L) of SAHA on the level of histone H4 acetylation. Ku70 and Bax protein expression in Patu8988 cells. Results: SAHA could inhibit the proliferation of Patu8988 cells in a concentration and time dependent, the IC50 of 48h was 5.40 µmol/L. The colony formation rate of SAHA cells treated with Patu8988 combined with radiotherapy was significantly lower than that of radiotherapy alone. The DO was 1.513, 2.229. Dq were 0.783 and 1.321. respectively, and the radiation enhancement ratio (SER) was 1.47. SAHA could increase the level of histone H4 acetylation in Patu8988 cells, inhibit the expression of DNA repair protein Ku70. and promote the expression of apoptosis related genes Bax in a dose-dependent maimer (P<0.05). Conclusion: SAHA could inhibit the proliferation of pancreatic cancer Patu898S cells in a concentration and time-dependent maimer and had radiosensitizing effect, it might be related to the inhibition of DNA double strand repair and promotion of apoptosis. [ABSTRACT FROM AUTHOR]

The study area was divided into four kinds of sampling plots as A (>100 cm),B (60-100 cm), C(40-60 cm), and D (20-40 cm) according to different topsoil thicknesses. The soil or coal gangue samples from different depths of reconstruction soil profile were collected to determine their water content (WC), pH value, bulk density (BD), organic carbon (SOC), microbial biomass carbon (MBC), and dissolved organic carbon (DOC). Thus the authors studied the effect of topsoil thickness on active organic carbon (AOC) distribution. The results show that the SOC content of soil layer 0-20 cm increases 0.48 g/kg in the test field after 5 a, while DOC and MBC average content is respectively 46.86 mg/kg,46.09 mg/kg. The recovery progress of organic carbon stock from the reclaimed soil in the experimental field is slower compared with other areas, which it is mainly subject to the nature of the reclamation soil and land utilization pattern. The SOC content of the same soil layer in the test zone is in relation to A>B>C>D, namely, the SOC content of the reclaimed soil filled with coal gangue also increases with the topsoil thickness increases. The MBC content of soil layer 0-20 cm in the sampling plots B and C is relatively higher, that too thick or thin for topsoil thickness are not conducive to the MBC accumulation of surface soil. The DOC content of coal gangue layer in plot D is as high as 97.90 mg/kg, and that of surface soil also reaches 64.80 mg/kg, which is higher than that of other sampling plots. The DOC of the topsoil may be affected by coal gangue layer when the topsoil thickness is thinner. The Soil qMBC value of coal gangue layer can not accurately reflect the reconstruction soil fertility quality, but the topsoil fertility quality and SOC can be represented with its MBC content.MBC and pH value, BD show a significant negative correlation (p<0.01), while it and WC have a significant positively correlation (p<0.05). The relationship of above mentioned soil physico-chemical properties and SOC, DOC is on the contrary to MBC. [ABSTRACT FROM AUTHOR]

This paper presented a statistics law on the same frequency words in Chinese text based on a large number of experiments. It deduced the mathematical expression of the same frequency words based on Zipf's law, which could be applied to Chinese text better. Moreover, it re-established the boundary points formula of high-frequency words and low-frequency words, and then verified its correctness. Finally, it applied the proposed statistics law to keywords extraction. Previous academic research on how to deal with low-frequency words was rare and nobody gave a concrete solution. This paper provided a standard method on how to deal with the low-frequency words in the application of keywords extraction. It notes that text length must be no less than 3 010 words and it can ignore the calculation of words occurring once and twice when calculating the value of TF-IDF. This method raises the efficiency by 2~7 times but no loss of keywords. [ABSTRACT FROM AUTHOR]

采用阳极氧化技术对不锈钢丝网表面进行修饰，成功制备了ZSM-5分子筛膜.通过XRD、SEM等技术对合成的材料进行表征，并考察了电解液种类、晶化时间、晶化温度以及合成次数等参数对分子筛膜的影响.结果表明，经阳极氧化工艺处理后，不锈钢丝网表面出现了明显的孔径结构，并生成了一层致密的阳极氧化膜.这些孔道结构和阳极氧化膜有利于ZSM-5分子筛膜的生长，是成功合成高结合力分子筛膜的关键.各制备参数对分子筛膜的结构形成亦产生了较大的影响.

采用溶胶凝胶法合成了尖晶石型LiMn2O4正极材料，选取钴、铈和镁作为掺杂元素，考察了钴、铈和镁的掺杂对LiMn2O4正极材料的结构和电化学性能的影响.并运用XRD、IR、BET等方法对所合成的材料进行了表征.实验结果表明，掺杂一定量钴、铈和镁后的LiMn2O4正极材料循环寿命优于未经掺杂的LiMn2O4正极材料，循环50次后，3种掺杂后所得的电池材料的容量保持率均达95%以上.其中以掺杂钴的LiCo0.2Mn1.8O4正极材料循环性能最好，首次充放电容量达到105.6 mAh/g，50次循环后，其充放电容量仍保持在100.8 mAh/g.

制备了用于气相选择性氧化制对叔丁基苯甲醛的催化剂.通过反应温度、进料浓度、空速、催化剂寿命等条件的考察,讨论了影响反应的因素.结果表明,在合适的反应条件下产物对叔丁基苯甲醛选择性可达80 mol%以上,反应物对叔丁基甲苯转化率大于12 mol%,且催化剂经365 h长时间寿命试验活性仍较稳定,无论从工业应用前景角度来看,还是在环境保护方面都具有很大意义.

通过浸渍法制备了一系列选择氧化制取对叔丁基苯甲醛的催化剂，采用O2-TPD、TPR表征技术考察了催化剂的表而氧和晶格氧的热脱附性能以及还原性能.实验结果表明助剂Cu、Tl的添加对催化剂表而氧性质影响较大，并发现催化剂的表而氧性质与其反应性能有很好的对应关系.