251. Enhancement of HIV-1 replication in peripheral blood mononuclear cells by Cryptococcus neoformans is monocyte-dependent but tumour necrosis factor-independent.
- Author
-
Orendi JM, Nottet HS, Visser MR, Verheul AF, Snippe H, and Verhoef J
- Subjects
- Antibodies, Monoclonal immunology, Cells, Cultured, Humans, Monocytes cytology, Monocytes physiology, Pentoxifylline pharmacology, Tumor Necrosis Factor-alpha immunology, Virus Replication, Cryptococcus neoformans physiology, HIV-1 physiology, Monocytes microbiology, Tumor Necrosis Factor-alpha metabolism
- Abstract
Objective: To investigate the possible role of Cryptococcus neoformans in HIV-1 pathogenesis., Design: An in vitro system was developed to study HIV-1 replication in freshly HIV-1-infected peripheral blood mononuclear cells (PBMC) incubated with whole azide-killed C. neoformans., Methods: Human PBMC or peripheral blood lymphocytes were infected with lymphocytotropic HIV-1 and incubated with azide-killed encapsulated or non-encapsulated C. neoformans for 10 days. Viral replication was followed by HIV-1 p24 enzyme-linked immunosorbent assay and median tissue culture infective dose determination. Tumour necrosis factor (TNF) release by PBMC, induced by C. neoformans, was measured. Anti-TNF monoclonal antibodies or pentoxifylline were used to inhibit TNF bioactivity., Results: Both encapsulated and non-encapsulated C. neoformans enhanced HIV-1 replication in PBMC but not in peripheral blood lymphocytes. C. neoformans induced TNF release by PBMC. Inhibition of TNF bioactivity did not block C. neoformans-enhanced HIV-1 replication in PBMC., Conclusions: C. neoformans can enhance HIV-1 replication in T cells only in the presence of monocytic cells. This enhancement is not dependent on encapsulation nor can it be attributed to TNF release.
- Published
- 1994
- Full Text
- View/download PDF