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Efficient induction of Semliki Forest virus and mumps virus neutralizing anti-anti-idiotypic antibodies using Quil A as adjuvant.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1991 Feb 15; Vol. 136 (2), pp. 169-75. - Publication Year :
- 1991
-
Abstract
- Rabbit anti-idiotypic sera were prepared against Semliki Forest virus (SFV) neutralizing monoclonal antibody (MAb) UM 1.13 and mumps virus neutralizing MAb UM 10B. From these sera anti-idiotypic antibodies were purified by ammonium sulphate precipitation and subsequent affinity column chromatography. Anti-iso- and anti-allotypic antibodies were removed by binding to normal mouse serum immunoglobulins coupled to CNBr activated Sepharose. Peak protein fractions eluted from columns loaded with homologous MAb were used for anti-anti-idiotypic immunization of BALB/c mice to raise virus neutralizing anti-anti-idiotypic antibodies. Two intracutaneous immunizations, five weeks apart, with affinity purified rabbit polyclonal anti-idiotypic antibody (40 micrograms protein per animal) coupled to keyhole limpet hemocyanin and mixed with the adjuvant Quil A (50 microliters per animal) were sufficient to evoke neutralizing antibodies against either virus. Moreover the mice who developed SFV neutralizing serum antibodies upon anti-idiotypic immunization all survived an otherwise lethal challenge with virulent SFV.
- Subjects :
- Adjuvants, Immunologic
Animals
Antibodies, Anti-Idiotypic biosynthesis
Antibodies, Anti-Idiotypic isolation & purification
Antibody Formation
Chromatography, Affinity
Female
Mice
Mice, Inbred BALB C
Quillaja Saponins
Immunization methods
Mumps virus immunology
Saponins therapeutic use
Semliki forest virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 136
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 1999649
- Full Text :
- https://doi.org/10.1016/0022-1759(91)90003-x