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Enhancement of HIV-1 replication in peripheral blood mononuclear cells by Cryptococcus neoformans is monocyte-dependent but tumour necrosis factor-independent.
- Source :
-
AIDS (London, England) [AIDS] 1994 Apr; Vol. 8 (4), pp. 423-9. - Publication Year :
- 1994
-
Abstract
- Objective: To investigate the possible role of Cryptococcus neoformans in HIV-1 pathogenesis.<br />Design: An in vitro system was developed to study HIV-1 replication in freshly HIV-1-infected peripheral blood mononuclear cells (PBMC) incubated with whole azide-killed C. neoformans.<br />Methods: Human PBMC or peripheral blood lymphocytes were infected with lymphocytotropic HIV-1 and incubated with azide-killed encapsulated or non-encapsulated C. neoformans for 10 days. Viral replication was followed by HIV-1 p24 enzyme-linked immunosorbent assay and median tissue culture infective dose determination. Tumour necrosis factor (TNF) release by PBMC, induced by C. neoformans, was measured. Anti-TNF monoclonal antibodies or pentoxifylline were used to inhibit TNF bioactivity.<br />Results: Both encapsulated and non-encapsulated C. neoformans enhanced HIV-1 replication in PBMC but not in peripheral blood lymphocytes. C. neoformans induced TNF release by PBMC. Inhibition of TNF bioactivity did not block C. neoformans-enhanced HIV-1 replication in PBMC.<br />Conclusions: C. neoformans can enhance HIV-1 replication in T cells only in the presence of monocytic cells. This enhancement is not dependent on encapsulation nor can it be attributed to TNF release.
- Subjects :
- Antibodies, Monoclonal immunology
Cells, Cultured
Humans
Monocytes cytology
Monocytes physiology
Pentoxifylline pharmacology
Tumor Necrosis Factor-alpha immunology
Virus Replication
Cryptococcus neoformans physiology
HIV-1 physiology
Monocytes microbiology
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0269-9370
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 8011245
- Full Text :
- https://doi.org/10.1097/00002030-199404000-00003