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软件定义光传送网采用集中式的控制器进行网络控制,控制器的性能及其测试方法是判断其是否存在性能"瓶颈"的重要手段。文章对评测控制器性能"瓶颈"的性能指标进行了分析,在此基础上,提出了一种针对控制器长期运行性能指标的测试方法,采用此方法可对控制器长期运行的稳定性、可靠性及性能进行综合评判。

本文介绍一个与现有300 MHz CATV电缆传输系统兼容的光缆传输系统,无中继距离可达30km,CNR可达43dB。文中还对国产LD—PIN器件提出了传输模拟信号的特殊要求。

【目的】构建载有小鼠抵抗素（resistin）基因及其反义核酸的重组真核表达质粒，探讨抵抗素对3T3-L1脂肪细胞葡萄糖摄取的影响。【方法】用RT-PCR技术从小鼠脂肪组织扩增抵抗素基因cDNA，A／T克隆于pGEM-T载体，再亚克隆于pcDNA3．1（＋）和pcDNA3．1（-）真核表达载体，构建载有小鼠抵抗素（resistin）基因及其反义核酸的重组真核表达质粒pcDNA3．1^（＋）resistin和pcDNA3．1^（-）-resistin^（-）。将pcDNA3．1^（-）resistin^（-）转染小鼠3T3-L1脂肪细胞，通过G418筛选稳定表达的细胞株。3T3-L1脂肪细胞分为对照、瞬时、载体和稳定4个细胞组（每组n=6），用^3H-2-脱氧葡萄糖进行非胰岛素和胰岛素介导的葡萄糖摄取试验。【结果】插入pcDNA3．1^（＋）的DNA片段的核苷酸序列与小鼠抵抗素基因mRNA编码区序列完全一致．且方向正确：插入pcDNA3．10^（＋）的DNA片段的核苷酸序列与抵抗素基因mRNA编码区核苷酸序列完全一致，且方向相反。对照组、瞬时组、载体组和稳定组的3T3-L1脂肪细胞非胰岛素和胰岛素介导的葡萄糖摄取率的无显著性差别（P〉O．05）。【结论】成功构建了载有抵抗素基因和载有抵抗素基因反义核酸的重组真核表达质粒。抵抗素对3T3-L1脂肪细胞的葡萄糖摄取均无明显影响。

【目的】探讨新诊断2型糖尿病患者血清炎症因子超敏-C反应蛋白（hs-CRP）、肿瘤坏死因子α（TNF-α）、白介素-6（IL-6）水平及外周血单个核细胞（PMNC）中NF-κB的活性变化。【方法】选择健康成人66例，新诊断2型糖尿病患者92例，分为正常对照组和2型糖尿病组，分析比较两组血清炎症因子及PMNC中NF-κB活性水平。血清hs-CRP、TNF-α、IL-6采用酶联免疫吸附法测定。免疫印迹分析检测PMNC中核因子KBp65（Set^536）的磷酸化水平，β-actin作为内参。【结果】2型糖尿病组患者的血清CRP、IL-6、INF-α水平均显著高于正常对照组（P〈0．001）；糖尿病组患者PMNC中NF-κB P65（Ser^536）磷酸化水平高于正常对照组（0．85±0．38vs0．47±0．25，P〈0．05）；在糖尿病组，log（CRP）与质量指数（BMI）、腰围、腰臀比、稳态模型法估计的胰岛素抵抗指数（HOMA-IR）、log（IL-6）、log（TNF-α）、糖基化血红蛋白（hemoglobin，Alc）呈正相关。【结论】结果提示2型糖尿病患者处于炎症状态，血清炎症因子水平及PMNC中NF-κB活性的升高可能与胰岛素抵抗和动脉粥样硬化的形成有关。

【目的】研究抵抗素（resistin）对脐静脉内皮细胞功能的影响，以探讨抵抗素在粥样硬化性疾病中的作用及其机制。【方法】原代培养人脐静脉内皮细胞，不同浓度人抵抗素（0、50、100ng／mL）培养24h。流式细胞检测抵抗素对内皮细胞间黏附分子（ICAM-1）、血管细胞间黏附分子（VCAM-1）与活性氧簇（ROS）表达的影响；RT-PCR检测抵抗素对内皮素（ET-1）、内皮型一氧化氮合酶（eNOS）、诱导型一氧化氮合酶（iNOS）mRNA表达的影响。【结果】人脐静脉内皮细胞经人抵抗素（50ng／mL、100ng／mL）处理24h后ICAM-1和ET-1mRNA表达显著增高，而各组间VCAM-1表达、ROS生成，以及eNOS和iNOS mRNA表达无明显差别。【结论】抵抗素可通过增加ICAM-1表达，上调ET-1表达直接促进内皮细胞激活，提示脂肪细胞与内皮细胞的相互作用可能是动脉粥样硬化性疾病的发病因素之一。

近年来，掺铒光纤放大器（ＥＤＦＡ）与波分复用（ＷＤＭ）相结合的传输技术已成为高速率、大容量光通信发展的主流方向。本文分析总结了国外研制适用于ＷＤＭ的增益平坦ＥＤＦＡ的几种有效方案，为适用于ＷＤＭ的ＥＤＦＡ实用化研究提供参考。

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As a common prosperity demonstration province, Zhejiang Province is facing the dilemma of traditional education methods in the cultivation of high-quality farmers, especially in the group of farmer college students. By sorting out the farmer training models of the advanced agricultural countries in Europe, such as Britain, France, the Netherlands and Germany, we summarized the references for farmer college students in Zhejiang Province, so as to explore the diversified governance paths for the cultivation of high-quality farmers in Zhejiang Province, and provide Zhejiang solutions for the cultivation of high-quality farmers in China. [ABSTRACT FROM AUTHOR]

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Due to the low resolution of spectroscopic instrument, the characteristics of elements with similar peak position overlap when detecting heavy metals in soil. Spectral overlapping peaks seriously affect the accuracy of quantitative analysis results. In order to obtain accurate heavy metal content, spectral overlapping peaks need to be decomposed. The African vulture algorithm was used to optimize the overlapping peaks of convolutional neural networks (AVOA-CNN). Firstly, 150 double Gaussian overlapping peaks and 43 triple Gaussian overlapping peaks with noise were simulated by Gaussian function model. Different wavelet basis functions were selected for spectral data denoising. With signal-to-noise ratio and root mean square error (RMSE) as evaluation indexes, coif 3 wavelet basis function was finally determined, and derivative method was used to pretreat spectral overlapping peaks. Then, AVOA-CNN was used to obtain the convolutional neural network (CNN) prediction results. The analytic results show that AVOA-CNN can decompose the overlapping peaks successfully and with high accuracy, and that the parameters of the double and triple Gaussian overlapping peaks (peak intensity, peak location, Peak width) are 3. 15% and 5. 90%, respectively. Finally, by comparing the sparrow search algorithm to optimize CNN, CNN and AVOA-CNN, the results show that the AVOA-CNN model has the highest prediction accuracy. [ABSTRACT FROM AUTHOR]

Transboundary water resource allocation is the core issue to alleviate water resources conflicts and realize effective management and utilization of water resources. Based on the perspective of equal negotiation, we used the Negotiation Game Model to describe the cooperation path among basin countries. Firstly, we introduced the idea of flexible weight to establish a fair and reasonable allocation mode by using a Multi-criteria Decision Model. Then, we used the negotiation game model to approximate the negotiation process of each basin country, to explore a fair and reasonable initial allocation scheme of transboundary water resources that can be generally recognized by each basin country. Taking the Lancang-Mekong River Basin (LMRB) as an example for empirical analysis, the results show that: ① The negotiation scheme proposed by each basin country based on the constraint of fair and reasonable allocation mode reflects the interest demands of each basin country, but the sum of the ideal water rights allocation ratio of each basin country does not meet the constraint condition equal to 100%, so negotiation is required. Each country bears a certain loss of water rights, and the reduction of water rights borne by each basin country ranges from zero to 5. 02%; ② The average satisfaction of the optimized allocation scheme under different preference scenarios is above 90%, which has higher satisfaction and stability compared with the negotiation scheme proposed by each basin country. It indicates that finding the optimal solution of water resource allocation based on the subject satisfaction function considers the interest demands of each basin country, which can improve the acceptability of the transboundary water rights allocation scheme; ③ Under different allocation scenarios, the proportion of transboundary water rights allocated to China and Thailand is higher, while the proportion allocated to Myanmar is lower. From the perspective of the change rate and the average satisfaction of each basin country before and after the negotiation, the transboundary water rights allocation scheme under the preference equity scenario and preference efficiency scenario is the less controversial scheme for each basin country. This study aims to improve the rationality and feasibility of transboundary water resource allocation and provide support for reducing water resource conflicts. [ABSTRACT FROM AUTHOR]

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