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This study elucidates methods for isolation and biochemical identification of thermophilic bacteria isolated from the Gauri Kund hot spring of Uttarakhand, India. Heavy metals are discharged from various industries and man-made activities into the soil and water resources. Increased toxic concentrations of heavy metals are responsible for disturbing metabolism and may cause lethal and chronic diseases in humans and plants. Therefore, this study aims to isolate and identify some metalresistant bacterial strains of thermophiles. A total of 3 iron (Fe) and 3 manganese (Mn) resistant bacterial strains were isolated. These strains were capable of tolerating the presence of Fe, and Mn metal ions. Additionally, the metal tolerance limit of isolated strains was determined by minimum inhibitory concentration (MIC). The thermophilic strain GA7 showed the highest MIC (475 μg/lt.) against Mn ions. However, for more accurate results, molecular characterization is also required and reported in another study with the results of a detailed bioremediation assay. The unique cell wall structures and thermoadaptation mechanisms of thermophilic bacteria help in heavy metal Bioremediation. It is concluded that bacterial bioremediation is a very good, eco-friendly, and cost-effective biotechnological approach to reduce heavy metal contamination from the soil and water bodies. [ABSTRACT FROM AUTHOR]

Background: Curcuma longa (Zingiberaceae), Azadirachta indica (Meliaceae), Psoralea corylifolia (Fabaceae), Pongamia pinnata (Fabaceae), and Woodfordia fruticosa (Lythraceae) stand as renowned medicinal plants in India. Traditionally, different parts of these plants have been employed for healing, particularly in the context of treating psoriasis and various other ailments. This catalyzed our investigation into the potential antipsoriatic properties of these botanicals. Objectives: We set out to evaluate the preliminary phytochemical analysis and in vitro antipsoriatic capabilities of acetone, ethanol, and water extracts derived from Curcuma longa, Azadirachta indica, Psoralea corylifolia, Pongamia pinnata, and Woodfordia fruticosa. Materials and Methods: The antipsoriatic potential of these extracts was assessed through the MTT assay, using HaCaT cells. Additionally, we conducted LDH assays and utilized confocal microscopy to gain further insights. Results: Our investigations unveiled the promising antiproliferative activity of these plants on skin keratinocytes. Notably, compounds A, B, and C demonstrated a significant decrease in cell viability compared to the control group. In contrast, compounds D and E did not exhibit significant differences compared to the control. Discussion and Conclusion: These findings substantiate the traditional uses of these plants in the treatment of psoriasis and underscore their potential as valuable resources in the quest for effective antipsoriatic treatments. [ABSTRACT FROM AUTHOR]

Objective To report experiences in Bihar, India's most densely populated state, with a state government programme to train community health workers (CHWs) to combat the coronavirus disease 2019 (COVID-19) pandemic in the state's predominantly rural population of 128 million. Methods In May 2021, during the second wave of the COVID-19 pandemic in India, the Bihari government initiated a 1-day COVID-19 training programme for rural, unaccredited CHWs who had recently completed a community health education course from the National Institute of Open Schooling. The use of primary health centre buildings and doctors to deliver COVID-19 training and the existence of certification data on CHWs who participated in the community health education course streamlined implementation and minimized costs. After COVID-19 training, CHWs were paid as first responders and COVID-19 treatment workers by the Bihari government. Findings Overall, 15 000 CHWs in Bihar completed the COVID-19 training programme in 2021 and a further 30 000 were enrolled. A survey of CHWs carried out after COVID-19 training had started found that 80% (81/102) were satisfied with training and felt they were receiving information from reliable sources. Conclusion The training and mobilization of a team of CHWs helped ease pressure on a stressed, rural, health-care system in Bihar and improved its preparedness for future COVID-19 outbreaks. The success of the training programme illustrates how local initiatives can help address gaps in the health workforce and extend the reach of public health care into rural areas, in addition to improving COVID-19 responses. [ABSTRACT FROM AUTHOR]

COVID‐19's second wave had a significant impact on India, on May 7, 2021, the largest daily recorded case count was a little more than 4 million, and it has since fallen. Although the number of new cases reported has dropped, during the third week of May 2021, India accounted for about 45% of new cases identified globally and around 34% of deaths. As India maintains its present level of stability, a new urgent threat has emerged in the form of coronavirus‐associated mucormycosis. Mucormycosis, an acute and deadly fungal infection caused by Mucorales‐related fungal species, is a fungal emergency with a particularly aggressive propensity for contiguous spread, associated with a poor prognosis if not properly and immediately identified, and treated. Mucormycosis, sometimes referred to as the "black fungus," has increased more rapidly in India during the second wave of COVID‐19 than during the first wave, with at least 14,872 cases as of May 28, 2021. Uncontrolled diabetic mellitus (DM) and other immunosuppressive diseases such as neutropenia and corticosteroid treatment have traditionally been identified as risk factors for mucormycosis. Therefore, the use of glucocorticoids or high doses of glucocorticoids in mild COVID‐19 cases (without hypoxemia) should be avoided. In addition, drugs that target the immune pathway, such as tocilizumab, are not recommended without clear benefits. [ABSTRACT FROM AUTHOR]

Objective: There is a high rate of death due to burns and wound infections in India. Innovators and manufacturers of wound and burn dressings need to keep themselves abreast of the most current rules governing medical devices. The concept of advanced dressings is emerging in India. However, local manufacturing is hindered by an absence of clear regulatory guidelines. The lack of advanced wound and burn dressings on the market critically affects patient survival rates, while imports from other countries make dressings unaffordable and beyond the reach of most patients. This article presents the details of an approval procedure for advanced dressings and provides manufacturers, innovators and investors with a better understanding of the classification rules, import, export and marketing approval processes. We also discus the reasons for high levels of imports and costs, as well as suggestions on how to address this. Method: A critical analysis was conducted of 54 research and review articles, including regulatory guidelines to control the marketing of wound and burn care dressings recommended by international regulatory agencies. Conclusion: India is highly dependent on imports of advanced dressings. Due to lack of clear, quality regulatory guidelines, innovators and manufacturers are facing issues while showcasing their product in the Indian market. Thus development of clear quality regulatory guidelines is crucial. Therefore, this article may guide and encourage manufacturers to develop advanced dressings in India [ABSTRACT FROM AUTHOR]

Objective: To assess the prevalence of diabetic cardiomyopathy in patients with diabetes mellitus in Manipur and its correlation with different parameters like obesity, blood pressure, lipids, duration of diabetes, and glycemic control. Materials and Methods: A total of 100 type 2 diabetic patients were selected randomly. Anthropometric parameters were recorded, blood glucose levels and lipid proiles were determined, and the echocardiographic examinations were performed in all patients according to standard techniques. Ejection fraction (EF) was calculated by the formula LVEF% = (LVID)2 - (LVIDS)2. Left ventricular EF was considered normal when EF was 55 to 75%. Diastolic dysfunction was calculated by measuring E and A transmitral inlow velocity. Left ventricular mass in grams is calculated by the formula LVM (gm) = 1.04 X 0.8 [(LVID + PWT + IVST)3 - LVID3] + 0.6. Results and Conclusions: Diabetic cardiomyopathy was found in 40 patients (40%) of the total study, 29 males (44.6%) and 11 females (31.4%). [ABSTRACT FROM AUTHOR]

• Idiopathic Scoliosis of young Indian population is reported by surface topography. • Results are compared with standard radiograph. • Strong correlation was found at kyphosis, lordosis & left vertebral rotation angle. • Moderate correlation was observed at left vertebral rotation angle. • ANOVA indicates significant relation between surface topography and radiography. In India, the measurement of Idiopathic Scoliosis (IS) is assessed by multiple radiographs. The long-term exposure of x-ray radiation ill effects. The Statico 3D system based on surface topography is compared the first time with radiography for the assessment of twenty-two subjects identified with IS. In the case of surface topography, a standard deviation of ±3.4 degrees and the reliability coefficient were found 0.996. At 95% confidence interval, a strong correlation (Pearson coefficient > 0.75) was observed in thoracic kyphosis, lumbar lordosis angle and left vertebral rotation while a moderate correlation (Pearson coefficient > 0.65) was measured in right vertebral rotation using radiograph and the Statico 3D system. ANOVA indicated a significant relation (p-value < 0.0001 and Alpha 0.05) for lumbar lordosis, thoracic kyphosis, vertebral rotation (right & left) between radiographs and surface topography using Statico 3D system. [ABSTRACT FROM AUTHOR]

Gait analysis is considered as the most systematic study of human motion. The analysis of gait includes visual and analytical perception of the individual, augmentation of various mechanical instrumentations for measuring movement of body, muscles activity and body mechanics. Past study focused on gait analysis of various animal locomotion and humans mainly on sports biomechanics. This paper aims to quantify the gait performance with Jaipur Knee, which is one of the most widely used prosthesis in Indian population. Gait data with Jaipur knee joint is not available till date. The proposed study targets to predict the performance of Jaipur knee joint in terms of gait symmetry with transfemoral Indian amputees. Gait symmetry may be the basis of recommendation of knee joint to prosthetic patients. This study used kinematics and kinetics parameters together to quantify the performance of Jaipur knee joint to evaluate gait symmetry. This research will be helpful for clinician to predict and further to prevent the degenerated musculoskeletal effects generally seen in unilateral transfemoral amputees. [ABSTRACT FROM AUTHOR]

Previous studies on mitochondrial DNA analysis of sheep from different regions of the world have revealed the presence of two major- A and B, and three minor- C, D and E maternal lineages. Lineage A is more frequent in Asia and lineage B is more abundant in regions other than Asia. We have analyzed mitochondrial DNA sequences of 330 sheep from 12 different breeds of India. Neighbor-joining analysis revealed lineage A, B and C in Indian sheep. Surprisingly, multidimensional scaling plot based on FST values of control region of mtDNA sequences showed significant breed differentiation in contrast to poor geographical structuring reported earlier in this species. The breed differentiation in Indian sheep was essentially due to variable contribution of two major lineages to different breeds, and sub- structuring of lineage A, possibly the latter resulting from genetic drift. Nucleotide diversity of this lineage was higher in Indian sheep (0.014 ± 0.007) as compared to that of sheep from other regions of the world (0.009 ± 0.005 to 0.01 ± 0.005). Reduced median network analysis of control region and cytochrome b gene sequences of Indian sheep when analyzed along with available published sequences of sheep from other regions of the world showed that several haplotypes of lineage A were exclusive to Indian sheep. Given the high nucleotide diversity in Indian sheep and the poor sharing of lineage A haplotypes between Indian and non-Indian sheep, we propose that lineage A sheep has also been domesticated in the east of Near East, possibly in Indian sub-continent. Finally, our data provide support that lineage B and additional lineage A haplotypes of sheep might have been introduced to Indian sub-continent from Near East, probably by ancient sea trade route. [ABSTRACT FROM AUTHOR]

• First report on Meta -topolin (m T)-mediated in vitro regeneration and phenolic acid biosynthesis in Crinum malabaricum. • Different concentrations of m T influenced the growth and development of C. malabaricum. • m T-treated cultures had higher levels of hydroxybenzoic and hydroxycinnamic acid derivatives. • This study will be helpful for large scale propagation and cultures can be manipulated to influence the production of phenolic acids. Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta -Topolin (m T) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 µM m T for six weeks, whereby 7.5 µM m T resulted in the maximum multiplication of adventitious shoots, much higher than the control. The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis, and it appeared that m T-treated cultures exhibited the highest concentration of phenolic acids. In particular, increased concentrations of gallic acid, protocatechuic acid, syringic acid, p -hydroxybenzoic acid, salicylic acid and vanillic acid were detected compared to the control. m T (2.5 and 5.0 µM) produced the maximum amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate the rising importance of m T for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medically important C. malabaricum. [ABSTRACT FROM AUTHOR]

Importance: A surge of COVID-19 occurred from March to June 2021, in New Delhi, India, linked to the B.1.617.2 (Delta) variant of SARS-CoV-2. COVID-19 vaccines were rolled out for health care workers (HCWs) starting in January 2021., Objective: To assess the incidence density of reinfection among a cohort of HCWs and estimate the effectiveness of the inactivated whole virion vaccine BBV152 against reinfection., Design, Setting, and Participants: This was a retrospective cohort study among HCWs working at a tertiary care center in New Delhi, India., Exposures: Vaccination with 0, 1, or 2 doses of BBV152., Main Outcomes and Measures: The HCWs were categorized as fully vaccinated (with 2 doses and ≥15 days after the second dose), partially vaccinated (with 1 dose or 2 doses with <15 days after the second dose), or unvaccinated. The incidence density of COVID-19 reinfection per 100 person-years was computed, and events from March 3, 2020, to June 18, 2021, were included for analysis. Unadjusted and adjusted hazard ratios (HRs) were estimated using a Cox proportional hazards model. Estimated vaccine effectiveness (1 - adjusted HR) was reported., Results: Among 15 244 HCWs who participated in the study, 4978 (32.7%) were diagnosed with COVID-19. The mean (SD) age was 36.6 (10.3) years, and 55.0% were male. The reinfection incidence density was 7.26 (95% CI: 6.09-8.66) per 100 person-years (124 HCWs [2.5%], total person follow-up period of 1696 person-years as time at risk). Fully vaccinated HCWs had lower risk of reinfection (HR, 0.14 [95% CI, 0.08-0.23]), symptomatic reinfection (HR, 0.13 [95% CI, 0.07-0.24]), and asymptomatic reinfection (HR, 0.16 [95% CI, 0.05-0.53]) compared with unvaccinated HCWs. Accordingly, among the 3 vaccine categories, reinfection was observed in 60 of 472 (12.7%) of unvaccinated (incidence density, 18.05 per 100 person-years; 95% CI, 14.02-23.25), 39 of 356 (11.0%) of partially vaccinated (incidence density 15.62 per 100 person-years; 95% CI, 11.42-21.38), and 17 of 1089 (1.6%) fully vaccinated (incidence density 2.18 per 100 person-years; 95% CI, 1.35-3.51) HCWs. The estimated effectiveness of BBV152 against reinfection was 86% (95% CI, 77%-92%); symptomatic reinfection, 87% (95% CI, 76%-93%); and asymptomatic reinfection, 84% (95% CI, 47%-95%) among fully vaccinated HCWs. Partial vaccination was not associated with reduced risk of reinfection., Conclusions and Relevance: These findings suggest that BBV152 was associated with protection against both symptomatic and asymptomatic reinfection in HCWs after a complete vaccination schedule, when the predominant circulating variant was B.1.617.2.

Medical devices, being life-saving tools, are considered to be a boon for healthcare system. However, in addition to their therapeutic effects, there are several ill consequences that are caused by these devices. An effective cohort vigilant system was needed to manage such adverse effects. This had led to the introduction of materiovigilance. Materiovigilance is the study and follow-up of occurrences that arise as a result from the usage of the medical equipment. It not only manages adverse events (AE) but also creates harmonization among countries. Keeping these objectives in focus, the principles, perspectives, and practices with regard to materiovigilance that are followed in the USA, Europe, China, Japan, Australia, Canada, and India are being compared. Such a comparison is essential, which will help us to understand the gaps in the current regulatory systems in the above-mentioned countries and furthermore will provide a comprehensive picture to the regulatory authorities to amend any existing laws if required. These amendments may ensure optimal patient safety by providing them a benign experience from the use of medical devices., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

This study represents the summary of the water quality of River Ganga during mass bathing in Haridwar during Maha Kumbha of 2010 in terms of microbiological and molecular analysis. The sample was collected from River Ganga during Makar Sankranti to Shakh Poornima and assessed for fecal indicator bacteria Escherichia colt along with Standard Plate Count (SPC) to determine total bacterial load in the river. Of all the nine days of sample collection (mass bathing days) results on the main royal bath (Baisakhi) displayed maximum SPC (log 6.79 cfu ml(-1)) and most probable number (210 and 150 MPN 100 ml(-1) for total and fecal coli form, respectively). The water was extremely contaminated and not suitable for drinking on Somvati Amavasya, Maghi Poornima, Maha Shivratri and Baisakhi. The results clearly indicated that the mass bathing coupled with ritual activities performed by bathers was most probable cause of increased values of different parameters. The polymerase chain reaction analysis targeting malate dehydrogenase (mdh) gene proved to be more rapid and sensitive than classical culture techniques.