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Increasing environmental concerns about organic waste in paper mill effluents demand alternative wastewater management technology. We reported novel activation of fulvic acid-like in paper mill effluents using hydrogen peroxide (H 2 O 2 ) as oxidizer and titanium oxide (TiO 2 ) as catalyst. Spectroscopic characteristics of fulvic acid-like in paper mill effluents before and after activation (PFA and PFA-Os, respectively) were compared with a benchmark fulvic acid extracted from leonardite (LFA). Results indicated that PFA-Os exhibited less lignin structures, more functional groups and lower molecular weight than PFA, sharing much similarity with LFA. Among PFA-Os with varying degrees of oxidation, PFA-O-3 activated with 1:2 vol ratio of paper mill effluent and 30% H 2 O 2 for 20 min digestion at 90 °C stands out to be the optimal for further examination of its biological activity. Bioassays with rice seed/seedling indicated that applications of LFA at 2-5 mg-C/L and PFA-O-3 at 60-100 mg-C/L significantly increased rice seed germination rate and seedling growth under salt stress imposed with 100 mM NaCl. The mechanism was mainly through reduced oxidative damage via activation of antioxidative enzymes and lipid peroxidation. This study provides the needed technical basis of safer and cleaner technologies for innovative management of paper mill effluents., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Paper mulberry is a high-quality woody feed resource plant with high crude protein content. It is widely distributed in China and has excellent characteristics of salt and alkali tolerance. Paper mulberry has ecological and economic importance. Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth. In the saline-alkali environments, transcriptome expression is altered leading to phenotypic defects in most plants. However, the regulatory mechanism related to paper mulberry's salt-stress (SS) response is not clearly understood. In the present study de novo transcriptomic assembly was performed, and gene expression levels were measured between different SS and natural conditions (25°C) as a control for paper mulberry plants. According to the results of our study, under NaCl stress conditions, the differential gene expression was observed in the leaves of paper mulberry compared with the control. A total of 2126 differentially expressed unigenes were observed. Among these unigenes the expression of 812 DEGs was up-regulated and the expression of 1,314 DEGs was down-regulated. Additionally, The GO and KEGG analyses regarding differentially expressed unigenes (DEUs) revealed that the observed critical transcriptomic alterations under salt stress (SS) conditions were associated with primary and secondary metabolism, photosynthesis, and plant hormone signaling pathways. Further investigations such as gene function studies regarding the unigenes depicting altered expression under salt stress conditions in paper mulberry will help understand the mechanism of salt tolerance, and this information can be utilized in paper mulberry breeding and improvement programs. [ABSTRACT FROM AUTHOR]

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Salinity is known to affect plant productivity by limiting leaf-level carbon exchange, root water uptake, and carbohydrates transport in the phloem. However, the mechanisms through which plants respond to salt exposure by adjusting leaf gas exchange and xylem-phloem flow are still mostly unexplored. A physically based model coupling xylem, leaf, and phloem flows is here developed to explain different osmoregulation patterns across species. Hydraulic coupling is controlled by leaf water potential, ψ l , and determined under four different maximization hypotheses: water uptake (1), carbon assimilation (2), sucrose transport (3), or (4) profit function - i.e. carbon gain minus hydraulic risk. All four hypotheses assume that finite transpiration occurs, providing a further constraint on ψ l . With increasing salinity, the model captures different transpiration patterns observed in halophytes (nonmonotonic) and glycophytes (monotonically decreasing) by reproducing the species-specific strength of xylem-leaf-phloem coupling. Salt tolerance thus emerges as plant's capability of differentiating between salt- and drought-induced hydraulic risk, and to regulate internal flows and osmolytes accordingly. Results are shown to be consistent across optimization schemes (1-3) for both halophytes and glycophytes. In halophytes, however, profit-maximization (4) predicts systematically higher ψ l than (1-3), pointing to the need of an updated definition of hydraulic cost for halophytes under saline conditions., (© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.)

Glutathione S-transferases (GSTs) have poorly understood roles in plant responses to environmental stresses. A polyethylene glycol (PEG)-induced tau class GST was identified in rice roots by protein microsequencing. PEG and the heavy metals Cd (20 μM), Zn (30 μM), Co and Ni rapidly and markedly induced osgstu4 and osgstu3 in rice seedling roots. Osgstu4 and osgstu3 were also induced in roots by hypoxic stress but not by cold nor heat shock. Salt stress and abscisic acid (ABA) rapidly induced osgstu3 in rice roots, whereas osgstu4 exhibited a late salt stress and no ABA response. Salicylic acid, jasmonic acid and the auxin α-naphthalene acetic acid triggered osgstu4 and osgstu3 expression. Osgstu4 and osgstu3 were rapidly and markedly induced by the antioxidant dithiothreitol and the strong oxidant hydrogen peroxide, which suggested that redox perturbations and reactive oxygen species are involved in their stress response regulations. [Copyright &y& Elsevier]

This study aimed to assess global trends in research on salt stress in rice and provide new directions for future studies. The subjects in this study are a plain text file with full records and cited references (Web of Science core collection as the database, "rice" and "salt" as the retrieved title with the date range from 1 January 2000 to 31 December 2021). The bibliometric method was used in this study, and the results were visualized using Scimago Graphica, VOSviewer, and CiteSpace. The results showed that China, India, and Japan contributed most of the literature in this field, and the institutes with the largest academic output were the Chinese Academy of Science, the International Rice Research Institute, and Nanjing Agriculture University. This study argues that research on salt stress in rice has been conducted in three main areas: phenotypes, response mechanisms, and remediation strategies. Inoculation of rhizosphere bacteria, ion homeostasis, soil remediation, and gene editing will be popular topics in rice salt stress research in the future. This study aimed to provide a potential theoretical direction for research on salt stress in rice as well as a reference for feasible studies on the exploitation of saline–alkali lands. [ABSTRACT FROM AUTHOR]

Soil salinity pose a significant challenge to global agriculture, threatening crop yields and food security. Understanding the salt tolerance mechanisms of plants is crucial for improving their survival under salt stress. AFP2, a negative regulator of ABA signaling, has been shown to play a crucial role in salt stress tolerance during seed germination. Mutations in AFP2 gene lead to increased sensitivity to salt stress. However, the underline mechanisms by which AFP2 regulates seed germination under salt stress remain elusive. In this study, we identified a protein interaction between AFP2 and SOS2, a Ser/Thr protein kinase known to play a critical role in salt stress response. Using a combination of genetic, biochemical, and physiological approaches, we investigated the role of the SOS2-AFP2 module in regulating seed germination under salt stress. Our findings reveal that SOS2 physically interacts with AFP2 and stabilizes it, leading to the degradation of the ABI5 protein, a negative transcription factor in seed germination under salt stress. This study sheds light on previously unknown connections within salt stress and ABA signaling, paving the way for novel strategies to enhance plant resilience against environmental challenges., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Large quantities of marine ballast water discharged by ocean-going vessels can cause salinity increases in freshwater ports, which in turn negatively affects indigenous plankton in the ports. In this study, we investigated the impacts of marine ballast water discharge on the plankton community in a freshwater wharf through field surveys. It was found that salinity stress caused reductions in community indicators such as plankton community composition, abundance and diversity, thus threatening the structure and function of the plankton community in the wharf. In terms of the impact range, the salinity stress had a significant effect on all plankton in the waters near the discharge point and the phytoplankton in the waters 50 m from the discharge point, but had no significant effect on the plankton in the waters further away. Ballast water discharge also caused a significant decrease in the alpha diversity and richness of the plankton community but had no significant effect on the evenness of the plankton community. Moreover, phytoplankton were more tolerant of salinity changes than zooplankton in our study. This study provides an ecological reference for the scientific management of marine ballast water discharge and the risk of exogenous nutrient inputs to freshwater ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Proanthocyanidins (PAs) are flavonoid compounds with important defensive roles in plants. The application of PAs in industries such as the pharmaceutical industry has led to increased interest in enhancing their biosynthesis. In Arabidopsis thaliana, PAs are biosynthesized under the regulation of an R2R3-MYB transcription factor TRANSPARENT TESTA 2 (TT2), which can interact with other proteins, including TRANSPARENT TESTA GLABRA 1 (TTG1), while also regulating a plant's response to abiotic stressors. However, the regulation of PA biosynthesis in the high-value medicinal plant Panax ginseng (ginseng) has not yet been studied. Understanding the mechanism of PAs biosynthesis regulation in ginseng may be helpful in increasing the plant's range of pharmacological applications. This study found that the overexpression of PgTT2 increased PA biosynthesis by an average of 67.3% in ginseng adventitious roots and 50.5% in arabidopsis seeds. Furthermore, transgenic arabidopsis plants overexpressing PgTT2 produced increased reactive oxygen species (ROS) scavenging ability by influencing abscisic acid synthesis and signaling. However, under high salinity stress, seed germination and growth rate of seedlings were decreased. An expression analysis of plants facing salt stress revealed increased transcripts of an ABA biosynthetic gene, NCED3, and ABA signaling genes ABI5 and ABI3. Moreover, the PgTT2 protein showed a direct interaction with PgTTG1 in yeast two-hybrid assays. This study therefore reveals novel information on the transcriptional regulation of PA production in ginseng and shows how PgTT2 influences the ABA response pathway to regulate responses to ROS and salt stress., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ok Ran Lee reports financial support was provided by National Research Foundation of Korea, Rural Development Administration, and Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Below-ground herbivory impacts plant development and often induces systemic responses in plants that affect the performance and feeding behavior of above-ground herbivores. Meanwhile, pest-damaged root tissue can enhance a plant's susceptibility to abiotic stress such as salinity. Yet, the extent to which herbivore-induced plant defenses are modulated by such abiotic stress has rarely been studied. In this study, we examine whether root feeding by larvae of the turnip moth, Agrotis segetum (Lepidoptera: Noctuidae) affects the performance of the above-ground, sap-feeding aphid Aphis gossypii (Hemiptera: Aphididae) on cotton, and assess whether those interactions are modulated by salinity stress. In the absence of salinity stress, A. segetum root feeding does not affect A. gossypii development. On the other hand, under intense salinity stress (i.e., 600 mM NaCl), A. segetum root feeding decreases aphid development time by 16.1 % and enhances fecundity by 72.0 %. Transcriptome, metabolome and bioassay trials showed that root feeding and salinity stress jointly trigger the biosynthesis of amino acids in cotton leaves. Specifically, increased titers of valine in leaf tissue relate to an enhanced performance of A. gossypii. Taken together, salinity stress alters the interaction between above- and below-ground feeders by changing amino acid accumulation. Our findings advance our understanding of how plants cope with concurrent biotic and abiotic stressors, and may help tailor plant protection strategies to varying production contexts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The crosstalk of phytohormones in the regulation of growth and development and the response of plants to environmental stresses is a cutting-edge research topic, especially in crop species. In this paper, we study the role and crosstalk between abscisic acid (ABA), ethylene (ET), and jasmonate (JA) in the control of germination and seedling growth in water or in standard nutrient solution and under salt stress (supplemented with 100-200 mM NaCl). The roles of ET and JA were studied using squash ET- and JA-deficient mutants aco1a and lox3a , respectively, while the crosstalk between ET, JA, and ABA was determined by comparing the expression of the key ABA, JA, and ET genes in wild-type (WT) and mutant genotypes under standard conditions and salt stress. Data showed that ET and JA are positive regulators of squash germination, a function that was found to be mediated by downregulating the ABA biosynthesis and signaling pathways. Under salt stress, aco1a germinated earlier than WT, while lox3a showed the same germination rate as WT, indicating that ET, but not JA, restricts squash germination under unfavorable salinity conditions, a function that was also mediated by upregulation of ABA. ET and JA were found to be negative regulators of plant growth during seedling establishment, although ET inhibits both the aerial part and the root, while JA inhibits only the root. Both aco1a and lox3a mutant roots showed increased tolerance to salt stress, a phenotype that was found to be mainly mediated by JA, although we cannot exclude that it is also mediated by ABA.

The Bacillus genus is widely distributed in nature, has bacteriostatic and growth-promoting activities, and has broad application potential in agriculture. An exopolysaccharide (EPS) was extracted and purified from Bacillus velezensis HY23. Structural characterisation of the EPS was performed by chemical and spectroscopic analyses. Methylation analysis showed that the EPS of HY23 was composed of mannose and glucose at a ratio of 82:18 and was identified as glucomannan. Combined with the nuclear magnetic resonance (NMR) analysis, EPS from HY23 had a backbone of →2)-α-D-Manp-(1 → and →2,6)-α-D-Manp-(1 → branched at C-6 with terminal α-(3-O-Me)-D-Manp-(1 → and →6)-α-D-Manp-(1 → residues as the side chain. A certain amount of β-D-Glcp residues were also present in backbone. Moreover, EPS significantly improved the nitrogen-fixing activity and salt resistance of soybean seedlings by regulating the antioxidant pool and expression of ion transporters. These findings indicate that EPS from B. velezensis HY23 is a potential biostimulant for enhancing plant resistance to salt stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

To explore the intrinsic influence of different salinity content on aniline biodegradation system in high temperature condition of 35 ± 1 °C, six groups at various salinity concentration (0.0%-5.0%) were applied. The results showed that the salinity exerted insignificant impact on aniline removal performance. The low-level salinity (0.5%-1.5%) stimulated the nitrogen metabolism performance. The G5-2.5% had excellent adaptability to salinity while the nitrogen removal capacity of G6-5.0% was almost lost. Moreover, high throughput sequencing analysis revealed that the g__norank_f__NS9_marine_group, g__Thauera and g__unclassified_f__Rhodobacteraceae proliferated wildly and established positive correlation each other in low salinity systems. The g__SM1A02 occupying the dominant position in G5 ensured the nitrification performance. In contrast, the Rhodococcus possessing great survival advantage in tremendous osmotic pressure competed with most functional genus, triggering the collapse of nitrogen metabolism capacity in G6. This work provided valuable guidance for the aniline wastewater treatment under salinity stress in high temperature condition., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The Manila clam (Ruditapes philippinarum) is a commercially important marine bivalve, which inhabits the estuarine and mudflat areas. The osmoregulation is of great significance for molluscs adaptation to salinity fluctuations. In this study, we investigated the effects of low salinity (10 psu) and high salinity (40 psu) stress on survival and osmoregulation of the R. philippinarum. The results of physiological parameters showed that the ion (Na + , K + , Cl - ) concentrations and Na + /K + -ATPase (NKA) activity of R. philippinarum decreased significantly under low salinity stress, but increased significantly under high salinity stress, indicating that there are differences in physiological adaptation of osmoregulation of R. philippinarum. In addition, we conducted the transcriptome analysis in the gills of R. philippinarum exposed to low (10 psu) and high (40 psu) salinity challenge for 48 h using RNA-seq technology. A total of 153 and 640 differentially expressed genes (DEGs) were identified in the low salinity (LS) group and high salinity (HS) group, respectively. The immune (IAP, TLR6, C1QL4, Ank3), ion transport (Slc34a2, SLC39A14), energy metabolism (PCK1, LDLRA, ACOX1) and DNA damage repair-related genes (Gadd45g, HSP70B2, GATA4) as well as FoxO, protein processing in endoplasmic reticulum and endocytosis pathways were involved in osmoregulation under low salinity stress of R. philippinarum. Conversely, the ion transport (SLC6A7, SLC6A9, SLC6A14, TRPM2), amino acid metabolism (GS, TauD, ABAT, ALDH4A1) and immune-related genes (MAP2K6, BIRC7A, CTSK, GVIN1), and amino acid metabolism pathways (beta-Alanine, Alanine, aspartate and glutamate, Glutathione) were involved in the process of osmoregulation under high salinity stress. The results obtained here revealed the difference of osmoregulation mechanism of R. philippinarum under low and high salinity stress through physiological and molecular levels. This study contributes to the assessment of salinity adaptation of bivalves in the context of climate change and provides useful information for marine resource conservation and aquaculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Expansin is a cell wall relaxant protein that is common in plants and directly or indirectly participates in the whole process of plant root growth, development and morphogenesis. A well-developed root system helps plants to better absorb water and nutrients from the soil while effectively assisting them in resisting osmotic stress, such as salt stress. In this study, we observed and quantified the morphology of the roots of Arabidopsis overexpressing the TaEXPAs gene obtained by the research group in the early stage of development. We combined the bioinformatics analysis results relating to EXPA genes in five plants and identified TaEXPA7-B , a member of the EXPA family closely related to root development in winter wheat. Subcellular localization analysis of the TaEXPA7-B protein showed that it is located in the plant cell wall. In this study, the TaEXPA7-B gene was overexpressed in rice. The results showed that plant height, root length and the number of lateral roots of rice overexpressing the TaEXPA7-B gene were significantly higher than those of the wild type, and the expression of the TaEXPA7-B gene significantly promoted the growth of lateral root primordium and cortical cells. The plants were treated with 250 mM NaCl solution to simulate salt stress. The results showed that the accumulation of osmotic regulators, cell wall-related substances and the antioxidant enzyme activities of the overexpressed plants were higher than those of the wild type, and they had better salt tolerance. This paper discusses the effects of winter wheat expansins in plant root development and salt stress tolerance and provides a theoretical basis and relevant reference for screening high-quality expansin regulating root development and salt stress resistance in winter wheat and its application in crop molecular breeding.

Soil salinity poses a substantial threat to agricultural productivity, resulting in far-reaching consequences. Green-synthesized lignin nanoparticles (LNPs) have emerged as significant biopolymers which effectively promote sustainable crop production and enhance abiotic stress tolerance. However, the defensive role and underlying mechanisms of LNPs against salt stress in Zea mays remain unexplored. The present study aims to elucidate two aspects: firstly, the synthesis of lignin nanoparticles from alkali lignin, which were characterized using Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Fourier Infrared Spectroscopy (FT-IR) and Energy Dispersive X-Ray Spectroscopy (EDX). The results confirmed the purity and morphology of LNPs. Secondly, the utilization of LNPs (200 mg/L) in nano priming to alleviate the adverse effects of NaCl (150 mM) on Zea mays seedlings. LNPs significantly reduced the accumulation of Na + (17/21%) and MDA levels (21/28%) in shoots/roots while increased lignin absorption (30/31%), resulting in improved photosynthetic performance and plant growth. Moreover, LNPs substantially improved plant biomass, antioxidant enzymatic activities and upregulated the expression of salt-tolerant genes (ZmNHX3 (1.52 & 2.81 FC), CBL (2.83 & 3.28 FC), ZmHKT1 (2.09 & 4.87 FC) and MAPK1 (3.50 & 2.39 FC) in both shoot and root tissues. Additionally, SEM and TEM observations of plant tissues confirmed the pivotal role of LNPs in mitigating NaCl-induced stress by reducing damages to guard cells, stomata and ultra-cellular structures. Overall, our findings highlight the efficacy of LNPs as a practical and cost-effective approach to alleviate NaCl-induced stress in Zea mays plants. These results offer a sustainable agri-environmental strategy for mitigating salt toxicity and enhancing crop production in saline environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Soil salinization and sodication harm soil fertility and crop production, especially in dry regions. To combat this, using biochar combined with gypsum, lime, and farm manure is a promising solution for improving salt-affected soils. In a pot experiment, cotton stick biochar (BC) was applied at a rate of 20 t/ha in combination with gypsum (G), lime (L), and farm manure (F) at rates of 5 and 10 t/ha. These were denoted as BCG-5, BCL-5, BCF-5, BCG-10, BCL-10, and BCF-10. Three different types of soils with electrical conductivity (EC) to sodium adsorption ratio (SAR) ratios of 2.45:13.7, 9.45:22, and 11.56:40 were used for experimentation. The application of BCG-10 led to significant improvements in rice biomass, chlorophyll content, and overall growth. It was observed that applying BCG-10 to soils increased the membrane stability index by 75% in EC:SAR (2.45:13.7), 97% in EC:SAR (9.45:22), and 40% in EC:SAR (11.56:40) compared to respective control treatments. After BCG-10 was applied, the hydrogen peroxide in leaves dropped by 29%, 23%, and 21% in EC:SAR (2.45:13.7), EC:SAR (9.45:22), and EC:SAR (11.56:40) soils, relative to their controls, respectively. The application of BCG-10 resulted in glycine betaine increases of 60, 119, and 165% in EC: SAR (2.45:13.7), EC: SAR (9.45:22), and EC: SAR (11.56:40) soils. EC: SAR (2.45:13.7), EC: SAR (9.45:22), and EC: SAR (11.56:40) soils all had 70, 109, and 130% more ascorbic acid in BCG-10 applied treatment, respectively. The results of this experiment show that BCG-10 increased the growth and physiological traits of rice plants were exposed to different levels of salt stress. This was achieved by lowering hydrogen peroxide levels, making plant cells more stable, and increasing non-enzymatic activity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Coastal ecotones can disrupt natural conditions, yielding intricate ecological contexts where salinity plays a variable role. The aim of this study was to assess the salinity effect on three species representatives of semifixed dune (Crucianella maritima, Helianthemum caput-felis and Teucrium dunense). Field data were collected to assess plant cover in semifixed dunes, ecotone with other coastal habitats, and artificial Posidonia oceanica wracks. Soil samples were collected, and conductivity measured. Then, experimental exposure to salinity was conducted with 6 seawater (SW) treatments (Control, 6.25 % SW, 12.5 % SW, 25 % SW, 50 % SW, 100 % SW). Flowering, gas exchange, chlorophyll fluorescence and enzymatic antioxidant measurements were conducted after two months of exposure. In the field trial, species presence varied depending on the habitat and was null on P. oceanica. The relation between conductivity and species abundance showed moderate tolerance for the three species. For C. maritima this relation was variable depending on the habitat. Experimental data suggest moderate tolerance with stress occurring at 25 % SW onwards. Gas exchange response to salinity was similar among species, but more drastic reduction in assimilation rate and larger decrease in water use efficiency was observed for C. maritima. Instead, photoinhibition occurred in H. caput-felis and T. dunense but was absent in C. maritima likely related to the fact that H. caput-felis and T. dunense activated catalase and superoxide dismutase enzymes, while C. maritima showed activation of glutathione-related enzymes. Malondialdehyde (MDA) increased in C. maritma and decreased for the other species indicating a more complex involvement of MDA under stress conditions. Flowering response to salinity was overall more resilient in T. dunense. Our results, based on field conductivity data and measurements of physiological, antioxidant, and reproductive traits, delineate specific tolerance differences and strategies towards salinity for Mediterranean semifixed dune species., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The Mediterranean basin is highly susceptible to climate change, with soil salinization and the increase in average temperatures being two of the main factors affecting crop productivity in this region. Following our previous studies on describing the detrimental effects of heat and salt stress co-exposure on tomato plants, this study aimed to understand if substrate supplementation with a combination of arbuscular mycorrhizal fungi (AMF) and biochar could mitigate the negative consequences of these stresses. Upon 21 days of exposure, stressed tomato plants grown under supplemented substrates showed increased tolerance to heat (42 °C for 4 h/day), salt (100 mM NaCl), and their combination, presenting increased biomass and flowering rate. The beneficial effects of AMF and biochar were associated with a better ionic balance (i.e. lower sodium accumulation and higher uptake of calcium and magnesium) and increased photosynthetic efficiency. Indeed, these plants presented higher chlorophyll content and improved CO 2 assimilation rates. Biochemical data further supported that tomato plants grown with AMF and biochar were capable of efficiently modulating their defence pathways, evidenced by the accumulation of proline, ascorbate, and glutathione, coupled with a lower dependency on energy-costly enzymatic antioxidant players. In summary, the obtained data strongly point towards a beneficial role of combined AMF and biochar as sustainable tools to improve plant growth and development under a climate change scenario, where soil salinization and heat peaks often occur together., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Cultivating productive paddy crops on salty soil to maximise production is a challenging approach to meeting the world's growing food demand. Thus, determining salinity tolerance rates in specific paddy cultivars is urgently needed. In this study, the salt tolerance traits of selected paddy cultivars, ADT45 and ADT39, were investigated by analysing germination, metabolites (pigments and biomolecules), and enzymatic (Superoxide dismutase (SOD), Catalase (CAT), and Peroxidase (POD) adaptation strategies as salt-stress tolerance mechanisms. This study found that salinity-induced reactive oxygen species (ROS) were efficiently detoxified by the antioxidant enzymes Superoxide dismutase (SOD), Catalase (CAT), and Peroxidase (POD) in ADT45 paddy varieties, followed by ADT39. Salinity stress had a significant impact on pigments and essential biomolecules in ADT45 and ADT39 paddy cultivars, including total chlorophyll, anthocyanin, carotenoids, ascorbic acid, hydrogen peroxide (H 2 O 2 ), malondialdehyde, and proline. ADT45 demonstrated a significant relationship between H 2 O 2 and antioxidant enzyme levels, followed by ADT39 paddy but not IR64. Morphological, physiological, and biochemical analyses revealed that ADT45, followed by ADT39, is a potential salt-tolerant rice cultivar., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Neurotransmitters are naturally found in many plants, but the molecular processes that govern their actions still need to be better understood. Acetylcholine, γ-Aminobutyric acid, histamine, melatonin, serotonin, and glutamate are the most common neurotransmitters in animals, and they all play a part in the development and information processing. It is worth noting that all these chemicals have been found in plants. Although much emphasis has been placed on understanding how neurotransmitters regulate mood and behaviour in humans, little is known about how they regulate plant growth and development. In this article, the information was reviewed and updated considering current thinking on neurotransmitter signaling in plants' metabolism, growth, development, salt tolerance, and the associated avenues for underlying research. The goal of this study is to advance neurotransmitter signaling research in plant biology, especially in the area of salt stress physiology., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

The challenge of desert farming with a high salt level has become an ecological task due to salt stress negatively affecting plant growth and reproduction. The current study deals with the cultivation of sorghum under salt stress conditions to counteract the effect of chitosan and gibberellic acid (GA 3 ). Here, the effects of chitosan, GA 3 and nano-composite (GA 3 @chitosan) on biochemical contents, growth and seed yield of sorghum under salinity stress conditions were studied. The results showed that spraying with GA 3 @chitosan increased sorghum grain yield by 2.07, 1.81 and 1.64 fold higher than salinity stressed plants, chitosan treatment and GA 3 treatment, respectively. Additionally, compared to the control of the same variety, the GA 3 @chitosan spraying treatment improved the concentration of microelements in the grains of the Shandweel-1 and Dorado by 24.51% and 18.39%, respectively for each variety. Furthermore, spraying GA 3 @chitosan on sorghum varieties increased the accumulation of the macroelements N, P, and K by 34.03%, 47.61%, and 8.67% higher than salt-stressed plants, respectively. On the other hand, the proline and glycinebetaine content in sorghum leaves sprayed with nano-composite were drop by 51.04% and 11.98% less than stressed plants, respectively. The results showed that, in Ras Sudr, the Shandweel-1 variety produced more grain per feddan than the Dorado variety. These findings suggest that GA 3 @chitosan improves the chemical and biochemical components leading to a decrease in the negative effect of salt stress on the plant which reflects in the high-yield production of cultivated sorghum plants in salt conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Populus euphratica phospholipase Dδ (PePLDδ) is transcriptionally regulated and mediates reactive oxygen species (ROS) and ion homeostasis under saline conditions. The purpose of this study is to explore the post-transcriptional regulation of PePLDδ in response to salt environment. P. euphratica PePLDδ was shown to interact with the NADP-dependent malic enzyme (NADP-ME) by screening the yeast two-hybrid libraries. The transcription level of PeNADP-ME increased upon salt exposure to NaCl (200 mM) in leaves and roots of P. euphratica. PeNADP-ME had a similar subcellular location with PePLDδ in the cytoplasm, and the interaction between PeNADP-ME and PePLDδ was further verified by GST pull-down and yeast two-hybrid. To clarify whether PeNADP-ME interacts with PePLDδ to enhance salt tolerance, PePLDδ and PeNADP-ME were overexpressed singly or doubly in Arabidopsis thaliana. Dual overexpression of PeNADP-ME and PePLDδ resulted in an even more pronounced improvement in salt tolerance compared with single transformants overexpressing PeNADP-ME or PePLDδ alone. Greater Na +  limitation and Na +  efflux in roots were observed in doubly overexpressed plants compared with singly overexpressed plants with PeNADP-ME or PePLDδ. Furthermore, NaCl stimulation of SOD, APX, and POD activity and transcription were more remarkable in the doubly overexpressed plants. It is noteworthy that the enzymic activity of NADP-ME and PLD, and total phosphatidic acid (PA) concentrations were significantly higher in the double-overexpressed plants than in the single transformants. We conclude that PeNADP-ME interacts with PePLDδ in Arabidopsis to promote PLD-derived PA signaling, conferring Na +  extrusion and ROS scavenging under salt stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

The olive tree (Olea europaea L.) is an evergreen tree that occupies 19% of the woody crop area and is cultivated in 67 countries on five continents. The largest olive production region is concentrated in the Mediterranean basin, where the olive tree has had an enormous economic, cultural, and environmental impact since the 7th century BC. In the Mediterranean region, salinity stands out as one of the main abiotic stress factors significantly affecting agricultural production. Moreover, climate change is expected to lead to increased salinization in this region, threatening olive productivity. Salt stress causes combined damage by osmotic stress and ionic toxicity, restricting olive growth and interfering with multiple metabolic processes. A large variability in salinity tolerance among olive cultivars has been described. This paper aims to synthesize information from the published literature on olive adaptations to salt stress and its importance in salinity tolerance. The morphological, physiological, biochemical, and molecular mechanisms of olive tolerance to salt stress are reviewed. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to investigate the important role of NAC gene in the growth and development of Cinnamomum camphora and its role in salt stress, and to clarify the response mechanism of C. camphora under salt stress. 【Method】The genome-wide NAC genes of C. camphora were identified and analyzed by bioinformatics to investigate the mode of response to abiotic stress in C. camphora. By identifying the members of NAC family of C. camphora,the paper aonducteda systematic analysis in terms of physicochemical properties, phylogenetic analysis, conserved motifs and gene structures, chromosomal localization and inter-species covariance, cis-acting element prediction and expression patterns.【Result】A total of 103 CcNAC genes were identified, the amino acid counts ranged from 137 to 1136, the isoelectric points from 4.54 to 10.00, the relative molecular masses from 15 644.40 to 129 859.29 u, and the mean hydrophobicity of all proteins was negative, indicating hydrophilicity. Subcellular localization was predicted to be in the nucleus and cytoplasm. A phylogenetic evolutionary tree was constructed with Arabidopsis thaliana NAC gene sequences, which classified CcNAC genes into 16 subfamilies. The NAM subfamily had the most members, with 17 CcNAC genes, while 25 CcNAC genes were not included. Conservative motif and gene structure analysis revealed that CcNAC genes were found to contain 2-8 introns and 10 motifs. Among these, motif3 appeared to be particularly stable in the genetic evolution of C. camphora genes, while motif9 and motif10 were distributed across the NAM subfamily. Chromosome localization and replication event analysis revealed that 103 genes were distributed across 12 chromosomes, with the highest number of genes（13） found in CcNAC genes distributed across chromosomes Chr02, with the lowest number of genes found on Chr10 and Chr11, which contained two genes each; Fragment duplication was the main mode of amplification for the CcNAC family. Multi-species co-linkage analysis revealed that CcNAC genes formed 162 pairs of co-linkage genes with CcNAC genes, indicating a closer relationship than with other species. The CcNAC genes were more closely related to the CcNAC genes and to those of other species than to any other. The CcNAC gene promoters contained several abiotic stress, growth and development, and hormone response elements. The results of the qRT-PCR showed that in leaves, the expression levels of the CcNAC058 and CcNAC092 genes were maximised under low salt stress. In roots, the expression of CcNAC007, CcNAC033, CcNAC058 and CcNAC092 genes increased with increasing salt concentration.【Conclusion】The NAC gene family may be involved in the process of salt stress response during the growth and development of C. camphora. The CcNAC092 and CcNAC058 genes exhibite significant responses in both leaves and roots under salt stress. It is hypothesised that CcNAC058 and CcNAC092 are the key genes contributing to the ability of the CcNAC to produce self-defensive behaviours under salt stress. [ABSTRACT FROM AUTHOR]

The motility behaviors at the individual-cell level and the collective physiological responsive behaviors of aerobic denitrifier, Enterobacter cloacae strain HNR under high salt stress were investigated. The results revealed that as salinity increased, electron transport activity and adenosine triphosphate content decreased from 15.75 μg O 2 /g/min and 593.51 mM/L to 3.27 μg O 2 /g/min and 5.34 mM/L, respectively, at 40 g/L, leading to a reduction in the rotation velocity and vibration amplitude of strain HNR. High salinity stress (40 g/L) down-regulated genes involved in ABC transporters (amino acids, sugars, metal ions, and inorganic ions) and activated the biofilm-related motility regulation mechanism in strain HNR, resulting in a further decrease in flagellar motility capacity and an increase in extracellular polymeric substances secretion (4.08 mg/g cell of PS and 40.03 mg/g cell of PN at 40 g/L). These responses facilitated biofilm formation and proved effective in countering elevated salt stress in strain HNR. Moreover, the genetic diversity associated with biofilm-related motility regulation in strain HNR enhanced the adaptability and stability of the strain HNR populations to salinity stress. This study enables a deeper understanding of the response mechanism of aerobic denitrifiers to high salt stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Rice straw breakdown is sluggish, which makes agricultural waste management difficult, however pretreatment procedures and cellulolytic fungi can address this issue. Through ITS sequencing, Chaetomium globosum C1, Aspergillus sp. F2, and Ascomycota sp. SM2 were identified from diverse sources. Ascomycota sp. SM2 exhibited the highest carboxymethyl cellulase (CMCase) activity (0.86 IU/mL) and filter-paper cellulase (FPase) activity (1.054 FPU/mL), while Aspergillus sp. F2 showed the highest CMCase activity (0.185 IU/mL) after various pretreatments of rice straw. These fungi thrived across a wide pH range, with Ascomycota sp. SM2 from pH 4 to 9, Aspergillus sp. F2, and Chaetomium globosum C1 thriving in alkaline conditions (pH 9). FTIR spectroscopy revealed significant structural changes in rice straw after enzymatic hydrolysis and solid-state fermentation, indicating lignin, cellulose, and hemicellulose degradation. Soil amendments with pretreated rice straw, cow manure, biochar, and these fungi increased root growth and soil nutrient availability, even under severe salt stress (up to 9.3 dS/m). The study emphasizes the need for a better understanding of Ascomycota sp. degradation capabilities and proposes that using cellulolytic fungus and pretreatment rice straw into soil amendments could mitigate salt-related difficulties and improve nutrient availability in salty soils. [ABSTRACT FROM AUTHOR]

The SnRK (sucrose non-fermentation-related protein kinase) plays an important role in regulating various signals in plants. However, as an important bamboo shoot and wood species, the response mechanism of PheSnRK in Phyllostachys edulis to hormones, low energy and stress remains unclear. In this paper, we focused on the structure, expression, and response of SnRK to hormones and sugars. In this study, we identified 75 PheSnRK genes from the Moso bamboo genome, which can be divided into three groups according to the evolutionary relationship. Cis-element analysis has shown that the PheSnRK gene can respond to various hormones, light, and stress. The PheSnRK2.9 proteins were localized in the nucleus and cytoplasm. Transgenic experiments showed that overexpression of PheSnRK2.9 inhibited root development, the plants were salt-tolerant and exhibited slowed starch consumption in Arabidopsis in the dark. The results of yeast one-hybrid and dual luciferase assay showed that PheIAAs and PheNACs can regulate PheSnRK2.9 gene expression by binding to the promoter of PheSnRK2.9. This study provided a comprehensive understanding of PheSnRK genes of Moso bamboo, which provides valuable information for further research on energy regulation mechanism and stress response during the growth and development of Moso bamboo. [ABSTRACT FROM AUTHOR]