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Populus euphratica PeNADP-ME interacts with PePLDδ to mediate sodium and ROS homeostasis under salinity stress.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 May; Vol. 210, pp. 108600. Date of Electronic Publication: 2024 Apr 04. - Publication Year :
- 2024
-
Abstract
- Populus euphratica phospholipase Dδ (PePLDδ) is transcriptionally regulated and mediates reactive oxygen species (ROS) and ion homeostasis under saline conditions. The purpose of this study is to explore the post-transcriptional regulation of PePLDδ in response to salt environment. P. euphratica PePLDδ was shown to interact with the NADP-dependent malic enzyme (NADP-ME) by screening the yeast two-hybrid libraries. The transcription level of PeNADP-ME increased upon salt exposure to NaCl (200 mM) in leaves and roots of P. euphratica. PeNADP-ME had a similar subcellular location with PePLDδ in the cytoplasm, and the interaction between PeNADP-ME and PePLDδ was further verified by GST pull-down and yeast two-hybrid. To clarify whether PeNADP-ME interacts with PePLDδ to enhance salt tolerance, PePLDδ and PeNADP-ME were overexpressed singly or doubly in Arabidopsis thaliana. Dual overexpression of PeNADP-ME and PePLDδ resulted in an even more pronounced improvement in salt tolerance compared with single transformants overexpressing PeNADP-ME or PePLDδ alone. Greater Na <superscript>+</superscript>  limitation and Na <superscript>+</superscript>  efflux in roots were observed in doubly overexpressed plants compared with singly overexpressed plants with PeNADP-ME or PePLDδ. Furthermore, NaCl stimulation of SOD, APX, and POD activity and transcription were more remarkable in the doubly overexpressed plants. It is noteworthy that the enzymic activity of NADP-ME and PLD, and total phosphatidic acid (PA) concentrations were significantly higher in the double-overexpressed plants than in the single transformants. We conclude that PeNADP-ME interacts with PePLDδ in Arabidopsis to promote PLD-derived PA signaling, conferring Na <superscript>+</superscript>  extrusion and ROS scavenging under salt stress.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Arabidopsis metabolism
Arabidopsis genetics
Gene Expression Regulation, Plant drug effects
Plant Roots metabolism
Plant Roots genetics
Plant Roots drug effects
Plants, Genetically Modified
Reactive Oxygen Species metabolism
Salt Tolerance genetics
Sodium Chloride pharmacology
Two-Hybrid System Techniques
Homeostasis
Phospholipase D metabolism
Phospholipase D genetics
Plant Proteins metabolism
Plant Proteins genetics
Populus metabolism
Populus genetics
Populus drug effects
Salt Stress genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2690
- Volume :
- 210
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 38593488
- Full Text :
- https://doi.org/10.1016/j.plaphy.2024.108600