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A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9 T , was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9 T grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9 T was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9 T formed a well-supported clade with Tersicoccus solisilvae CGMCC 1.15480 T and Tersicoccus phoenicis 1P05MA T and shared the highest similarity to T. solisilvae CGMCC 1.15480 T (98.9% sequence similarity) and T. phoenicis 1P05MA T (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9 T and T. solisilvae CGMCC 1.15480 T gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C 15:0 , anteiso-C 17:0 and iso-C 16:0 . The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H 2 ) and MK-9(H 2 ) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9 T represents a novel species of the genus Tersicoccus . The name Tersicoccus mangrovi sp. nov. is proposed, with the type strain MR15.9 T (=MCCC 1K08875 T =KCTC 59105 T ).

A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3 T , was isolated from a branch of Kandelia candel , collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CBK3Z-3 T grew at pH 5.0-10.0 (optimum, pH 8.0), 20-37 °C (optimum, 25-30 °C) and with 0-10 % (w/v) NaCl (optimum, 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBK3Z-3 T was closely related to species of genus Stakelama and had the highest 16S rRNA gene sequence similarity of 98.7 % to Stakelama pacifica CGMCC 1.7294 T . The DNA G+C content value of strain CBK3Z-3 T was 62.6 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was meso -diaminopimelic acid and the polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, an unidentified aminolipid and an unidentified lipid. The major fatty acids were C 18 : 1  ω 7 c and C 16 : 0 . The average nucleotide identity, estimated digital DNA-DNA hybridization and average amino acid identity values between strain CBK3Z-3 T and the type strain of Stakelama pacifica CGMCC 1.7294 T were 80.4, 23.1 and 81.5 %, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain CBK3Z-3 T should be designated as a novel species of the genus Stakelama , for which the name Stakelama flava sp.nov. is proposed. The type strain is CBK3Z-3 T (=JCM 34534 T =CGMCC 1.18972 T ).

A Gram-stain-positive, aerobic, non-motile, non-spore-forming and coccus-shaped strain, designated strain G463 T , was isolated from the rhizosphere soil of Salicornia europaea L. collected from Lake Gudzhirganskoe in Siberia. Based on 16S rRNA gene phylogeny, strain G463 T belonged to the genus Hoyosella , with the highest 16S rRNA gene sequence similarity to Hoyosella altamirensis DSM 45258 T (96.1%). The major fatty acids were C 17:1 ω 8 c , C 16:0 , C 15 : 0 and C 17:0 . The strain contained meso -diaminopimelic acid as the cell-wall diagnostic diamino acid and arabinose, galactose and ribose as the whole-cell sugars. MK-8 and MK-7 were the predominant menaquinones. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phosphoglycolipid, two unidentified glycolipids and several unidentified lipids. Acetyl was the muramyl residue. Mycolic acids (C 28 -C 34 ) were present. The G+C content of the genomic DNA was 68.3 mol%. Based on its phylogenetic, phenotypic and chemotaxonomic features, strain G463 T was considered to represent a novel species of the genus Hoyosella , for which the name Hoyosella lacisalsi sp. nov. is proposed. The type strain is G463 T (=JCM 33650 T =CGMCC 1.17230 T ).

A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2 T , was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China. Strain CMS6Z-2 T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %). Strain CMS6Z-2 T possessed meso -diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H 4 ) as the predominant menaquinone. The major fatty acids were iso-C 15 : 0 , C 16 : 0 and C 18 : 1  ω 9 c . The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The G+C content of the genomic DNA was 74.1 mol%. Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2 T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6 T with 98.3 % similarity. Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2 T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6 T within the genus Phycicoccus . The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2 T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2 T represents a novel species of the genus Phycicoccus , for which the name Phycicoccus flavus sp. nov. is proposed. The type strain is CMS6Z-2 T (=KCTC 49240 T =CGMCC4.7549 T ).

A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435 T , was isolated from soil sample collected from the Cholistan Desert, Pakistan. The taxonomic position of the strain was established by using a polyphasic taxonomic approach. The cells were coccoid-shaped and found in single or arrangement of pairs. The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %). Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2 T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711 T (95.2 %). In phylogenetic trees, strain F435 T formed a robust cluster with the only member of the genus Auraticoccus . The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid. The major fatty acid was determined to be anteiso-C 15 : 0 . Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinone was MK-9(H 4 ). The genomic G+C content was calculated as 73.5 mol%. The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435 T and A. monumenti MON 2.2 T were 24.6 and 81.8 %, respectively. Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435 T represents a novel specie of the genus Auraticoccus , for which the name Auraticoccus cholistanensis sp. nov. is proposed. The type strain is F435 T (=JCM 33648 T =CGMCC 1.17443 T ). The description of the genus Auraticoccus has also been emended.

A novel actinobacterial strain, designated 18 T , was isolated from a steppe soil sample collected in Buryatia, Russia and subjected to polyphasic taxonomic characterization. The strain was aerobic and Gram-stain-positive. The 16S rRNA gene sequence of strain 18 T exhibited highest similarity to Glycomyces paridis CPCC 204357 T (97.2 %). Results of phylogenetic analysis showed that strain 18 T formed a distinct branch clearly affiliated to the genus Glycomyces . Whole-cell hydrolysates of the isolate contained meso -diaminopimelic acid as the cell-wall diamino acid. The whole-cell sugar profile was found to contain galactose, glucose, ribose and xylose. MK-10(H 4 ) and MK-11(H 4 ) were the predominant menaquinones. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and four unidentified polar lipids. The major fatty acids identified were anteiso-C 15 : 0 and iso-C 15 : 0 . The G+C content of the genomic DNA of strain 18 T was 68.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 18 T was considered to represent a novel species of the genus Glycomyces , for which the name Glycomyces buryatensis sp. nov. is proposed. The type strain of Glycomyces buryatensis is 18 T (=JCM 33362 T =CGMCC 4.7610 T ).

A novel actinobacterium, designated strain 16Sb5-5 T , was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped. Strain 16Sb5-5 T grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl. The cell-wall peptidoglycan was of the A3 γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP). Ribose, arabinose and glucose were detected in the whole-cell hydrolysates. The predominant menaquinone was MK-9(H 4 ). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids. The major whole-cell fatty acids were anteiso-C 15 : 0 and iso-C 15 : 0 . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5 T was closely related to Desertihabitans aurantiacus CPCC 204711 T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees. In silico genomic comparisons showed that strain 16Sb5-5 T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711 T . The genomic G+C content was 73.3 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5 T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans , for which the name Desertihabitans brevis sp. nov. is proposed. The type strain is 16Sb5-5 T (=KCTC 49116 T =CGMCC 1.16553 T ). The description of the genus Desertihabitans has also been emended.

A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5 T , was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach. Strain 21Sc5-5 T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5 T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7 T (97.30 %), followed by Nocardioides kongjuensis A2-4 T (97.22 %), Nocardioides nitrophenolicus NSP 41 T (97.15 %) and Nocardioides caeni MN8 T (97.15 %). The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H 4 ) as the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids. The major fatty acids were iso-C 16 : 0 and 10-methyl C 18 : 0 . The genome length of strain 21Sc5-5 T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%. On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5 T represents a novel species of the genus Nocardioides , for which the name Nocardioides vastitatis sp. nov. is proposed. The type strain is 21Sc5-5 T (=JCM 33365 T =CGMCC 4.7608 T ).

Two novel strains, designated 11W25H-1 T and 8H24J-4-2 T , were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China. The strains were characterized by a polyphasic approach in order to clarify their taxonomic positions. They were Gram-stain positive, aerobic, non-motile, non-spore-forming and rod-shaped. The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176 T (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961 T (98.9% and 99.0%, respectively). The sequence similarity between strains 11W25H-1 T and 8H24J-4-2 T was 99.4%. Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176 T and L. endophytica CPCC 203961 T . Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation. The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species. The data demonstrated that strains 11W25H-1 T and 8H24J-4-2 T represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp. nov. (type strain 11W25H-1 T =JCM 33144 T =CGMCC 1.16700 T ) and Labedella populi sp. nov. (type strain 8H24J-4-2 T =JCM 33143 T =CGMCC 1.16697 T ) are proposed., (Copyright © 2019 Elsevier GmbH. All rights reserved.)

A Gram-stain-positive, aerobic, coccoid-shaped, non-spore-forming actinobacterial strain, designated M5W7-7 T , was isolated from a hot spring soil sample collected from Anshan, Liaoning province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M5W7-7 T clustered closely with species of the genus Kocuria, and showed the highest sequence similarity of 97.1 % to Kocuria subflava YIM 13062 T . Strain M5W7-7 T grew at 10-37 °C (optimum, 37 °C), pH6.0-11.0 (pH 6.0-7.0) and in the presence of 0-7 % (w/v) NaCl (0 %). Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested. Strain M5W7-7 T contained MK-6(H2) and MK-7(H2) as the dominant menaquinones. The polar lipid profile of strain M5W7-7 T contained diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The predominant whole-cell sugars were galactose and glucose. The predominant fatty acid was anteiso-C15 : 0. The DNA G+C content of strain M5W7-7 T was 67.0 mol%. On the basis of phylogenetic relationships, phenotypic characterization and chemotaxonomic analyses, strain M5W7-7 T represents a novel species of the genus Kocuria, for which the name Kocuriasoli sp. nov. is proposed. The type strain is M5W7-7 T (=KCTC 49195 T =CGMCC 1.13744 T ).

A Gram-stain-positive, aerobic, motile, non-spore-forming and rod-shaped actinobacterium, designated strain 4Q3S-7 T , was isolated from a piece of surface-sterilized bark of Kandelia candel collected at the Cotai Ecological Zone in Macao, China. Colonies were yellowish white, circular, smooth and convex. The 16S rRNA gene sequence of strain 4Q3S-7 T exhibited highest similarities to Marmoricola ginsengisoli Gsoil 097 T (97.6 %), Marmoricola solisilvae KIS18-7 T (97.6 %) and Marmoricola pocheonensis Gsoil 818 T (97.3 %). Phylogenetic analysis showed that strain 4Q3S-7 T clustered with species of the genus Marmoricola and was clearly affiliated to the genus Marmoricola. Genomic analyses, including average nucleotide identity and DNA-DNA hybridization, clearly separated strain 4Q3S-7 T from M. ginsengisoli Gsoil 097 T , M. solisilvae KIS18-7 T and M. pocheonensis Gsoil 818 T with values below the thresholds for species delineation. Strain 4Q3S-7 T had ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall. The major fatty acids (>10 % of total fatty acids) were C18 : 0 10-methyl (TBSA), C18 : 1ω9c, iso-C16 : 0 and iso-C16 : 0 2-OH. The predominant menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content of strain 4Q3S-7 T was 72.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 4Q3S-7 T is considered to represent a novel species of the genus Marmoricola, for which the name Marmoricola mangrovicus sp. nov. is proposed. The type strain is 4Q3S-7 T (=KCTC 39790 T =CGMCC 4.7424 T ).

A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3 T , was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Strain 13S1-3 T grew optimally at 28-30 °C, pH 7.0 and with 0-1 % (w/v) NaCl. The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid. Ribose, xylose, glucose and galactose were detected as cell-wall sugars. The acyl type of the muramic acid was acetyl. The predominant menaquinones were MK-12, MK-11, MK-13 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid. The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 70.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3 T represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02 T (97.11 %). Phylogenetic trees revealed that 13S1-3 T formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3 T is distinguishable from phylogenetically related genera in the family Microbacteriaceae, and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp. nov. is proposed. The type strain is 13S1-3 T (=KCTC 49115 T =CGMCC 1.16554 T ).

A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5 T , was isolated from a surface-sterilized bark of Neriumindicum Mill. collected from Guizhou, China, and investigated by a polyphasic approach to determine its taxonomic position. Strain M8JJ-5 T grew optimally without NaCl at 28 °C and at pH 7.0-8.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5 T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037 T . The DNA G+C content of strain M8JJ-5 T was 68.6 mol%. The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5 T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp. nov. is proposed. The type strain is M8JJ-5 T (=KCTC 49089 T =CGMCC 1.16390 T ).

A Gram-stain-positive, aerobic, non-motile, non-spore-forming, coccus-shaped actinobacterium, designated strain 12Sc4-1 T , was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China. Strain 12Sc4-1 T grew at 8‒35 °C (optimum, 28‒30 °C), pH 6.0‒9.0 (optimum, pH 7.0) and in the presence of 0‒3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 12Sc4-1 T belonged to the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity to Nakamurella silvestris S20-107 T (96.94 %). Strain 12Sc4-1 T showed <96.0 % 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella. Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, glucose, mannose and galactose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, phosphatidylinositol, an unidentified phospholipid, an unidentified phosphoglycolipid and an unidentified glycolipid. The major fatty acids were C16 : 0 and anteiso-C15 : 0. The DNA G+C content was 72.1 mol% (draft genome sequence). On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 12Sc4-1 T represents a novel species of the genus Nakamurella, for which the name Nakamurelladeserti sp. nov. is proposed. The type strain is 12Sc4-1 T (=KCTC 49114 T =CGMCC 1.16555 T ).

A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2 T , was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position. Strain M1HQ-2 T grew at 4-37 °C (optimum, 30 °C), pH 5.0-11.0 (pH 8.0) and in the presence of 0-15 % (w/v) NaCl (1-3 %). Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2 T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3 T . Strain M1HQ-2 T contained MK-7 as the dominant menaquinone. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipids profile of strain M1HQ-2 T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid. The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content of strain M1HQ-2 T was 71.0 mol%. The average nucleotide identity value between strain M1HQ-2 T and type strain of Brachybacterium sacelli was 76.7 %. The estimated DNA-DNA hybridization value between strain M1HQ-2 T and type strain of B. sacelli was 20.6 %. On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2 T represents a novel species of the genus Brachybacterium, for which the name Brachybacteriumendophyticum sp. nov. is proposed. The type strain is M1HQ-2 T (=KCTC 49087 T =CGMCC 1.16391 T ).

A Gram-negative, aerobic, motile and short-rod-shaped bacterium, designated strain 5T4P-12-1 T , was isolated from a piece of surface-sterilized bark of Aegiceras corniculatum collected from Cotai Ecological Zones in Macao, China and tested by a polyphasic approach to clarify its taxonomic position. Strain 5T4P-12-1 T grew optimally with 0-1 % (w/v) NaCl at 30 °C and at pH 7.0-8.0. The 16S rRNA gene sequence of strain 5T4P-12-1 T had the highest similarity (96.7 %) to Aureimonas altamirensis DSM 21988 T . Phylogenic analysis based on 16S rRNA gene sequences and coding sequences of 98 protein clusters showed that the strain represented a novel genus of the family Aurantimonadaceae. The predominant quinone system of strain 5T4P-12-1 T was ubiquinone 10. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unidentified aminolipids, three unidentified phospholipids and three unidentified lipids. The major fatty acids (>10 % of total fatty acids) were C18 : 1ω7c (55.4 %) and C18 : 1 2-OH (15.6 %). The DNA G+C content of strain 5T4P-12-1 T was 66.5 mol%. Based on the phylogenic, phenotypic and chemotaxonomic features, strain 5T4P-12-1 T is considered to represent a novel species of a new genus in the family Aurantimonadaceae, for which the name Mangrovicella endophytica gen. nov., sp. nov. is proposed. The type strain is 5T4P-12-1 T (=KCTC 62053 T =CGMCC 1.16279 T ).

A Gram-stain-positive, aerobic, non-motile, non-spore-forming and short-rod-shaped actinobacterium, designated strain 1T4Z-3 T , was isolated from a piece of surface-sterilized branch of Aegiceras corniculatum collected from the Cotai Ecological Zones in Macao, China. Comparative 16S rRNA gene sequence analysis showed that strain 1T4Z-3 T was clearly affiliated to the genus Amnibacterium and exhibited 97.9 % gene sequence similarity to Amnibacterium kyonggiense JCM 16463 T , 97.3 % gene sequence similarity to Amnibacterium soli JCM 19015 T and less than 96.4 % gene sequence similarities to other genera of the family Microbacteriaceae. Strain 1T4Z-3 T had L-2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid. The major fatty acids (>10 % of total fatty acids) were iso-C16 : 0 (46.6 %) and anteiso-C15 : 0 (27.3 %). The predominant menaquinones of strain 1T4Z-3 T were MK-11 (81.4 %) and MK-12 (14.1 %). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids, four unidentified phospholipids and two unidentified lipids. The DNA G+C content of strain 1T4Z-3 T was 71.4 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain 1T4Z-3 T is considered to represent a novel species of the genus Amnibacterium, for which the name Amnibacterium endophyticum sp. nov. is proposed. The type strain of Amnibacterium endophyticum is 1T4Z-3 T (=KCTC 39983 T =CGMCC 1.16066 T ).

A novel endophytic actinobacterium, designated strain 8BXZ-J1 T , was isolated from surface-sterilized branches of Thespesia populnea collected from Beilun Estuary Mangrove Forest National Nature Reserve in Guangxi, China, and examined by a polyphasic approach to determine its taxonomic position. Cells of the isolate were Gram-stain-positive, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 8BXZ-J1 T belonged to the genus Marmoricola, sharing highest similarity with Marmoricola solisilvae DSM 27140 T (96.9 %). The isolate grew at 10-35 °C (optimum, 28-30 °C), at pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 0-10 % (w/v) NaCl (optimum, 0-5.0 %). The organism contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, MK-8(H4) as the major menaquinone, and a polar lipid profile including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown lipids. The major fatty acids of strain 8BXZ-J1 T were C18 : 0 10-methyl, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA was 68.7 mol%. These data demonstrate that strain 8BXZ-J1 T is representative of a novel species of the genus Marmoricola, for which the name Marmoricola endophyticus sp. nov. is proposed. The type strain is 8BXZ-J1 T (=KCTC 39789 T =CGMCC 1.16067 T ).

A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain 2T4P-2-4T, was isolated from a piece of surface-sterilized bark of Aegiceras corniculatum collected from Cotai Ecological Zones in Macao, China, and tested by a polyphasic approach to clarify its taxonomic position. Strain 2T4P-2-4T grew optimally without NaCl at 28-30 °C, pH 7.0-8.0. The 16S rRNA gene sequence of strain 2T4P-2-4T had the highest similarity (96.2 %) to Aureimonas rubiginis CC-CFT034T. Phylogenetic analysis showed that the strain grouped with species of the genus Aureimonas. The predominant quinone system of strain 2T4P-2-4T was ubiquinone 10 (Q-10). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sulfoquinovosyldiacylglycerol, phosphatidylmethylethanolamine, two unidentified amino lipids, an unidentified aminophospholipid and five unidentified lipids. The predominant cellular fatty acid was C18 : 1ω7c (61.2 %). The DNA G+C content of strain 2T4P-2-4T was 69.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain 2T4P-2-4T is a representative of a novel species of the genus Aureimonas, for which the name Aureimonas endophytica sp. nov. is proposed. The type strain of Aureimonas endophytica sp. nov. is 2T4P-2-4T (=KCTC 52217T=CGMCC 1.15367T).

A novel endophytic actinobacterium, designated strain S3Cf-2T, was isolated from a surface-sterilized bark of Bruguiera sexangula collected from Dongzhaigang National Nature Reserve in Hainan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain S3Cf-2T fell within the family Micromonosporaceae and formed a distinct clade in the Micromonosporaceae phylogenetic tree. The 16S rRNA gene sequence similarity values between strain S3Cf-2T and the type species of 30 genera in the family Micromonosporaceae were 91.55-97.45 %. Strain S3Cf-2T formed extensively branched substrate mycelia without fragmentation. An oval or rod-like spore with a smooth surface was borne singly at the end of substrate mycelium. The novel isolate possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan, and glucose, galactose, mannose, arabinose, xylose and ribose as whole-cell sugars. The acyl type of the cell-wall peptidoglycan was glycolyl and mycolic acids were absent. The major polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown aminolipid, corresponding to phospholipid type PII. The major menaquinones were MK-9(H6) and MK-9(H8). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 71.4 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain S3Cf-2T represents a novel species of a new genus within the family Micromonosporaceae, for which the name Mangrovihabitans endophyticus gen. nov., sp. nov. is proposed. The type strain of the type species is S3Cf-2T (=DSM 100693T=CGMCC 4.7299T).

A coccus-shaped, non-spore-forming actinobacterium, designated strain 4Q3S-3T, was isolated from surface-sterilized bark of the mangrove plant Kandelia candel collected from Cotai Ecological Zones in Macao, China, and tested by a polyphasic approach to clarify its taxonomic position. This actinobacterium was Gram-stain-positive and aerobic. Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested. Strain 4Q3S-3T grew optimally without NaCl at 28-30 °C, pH 7.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain 4Q3S-3T belonged to the genus Friedmanniella and shared the highest 16S rRNA gene sequence similarity with Friedmanniellaflava W6T (96.57 %). The DNA G+C content of strain 4Q3S-3T was 69.5 mol%. The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid, and MK-9(H4) was the predominant menaquinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, unidentified glycolipid, amino lipids and two unidentified phospholipids. The predominant fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. Based on phylogenetic, phenotypic and chemotaxonomic data, strain 4Q3S-3T represents a novel species of the genus Friedmanniella, for which the name Friedmanniella endophytica sp. nov. is proposed. The type strain is 4Q3S-3T (=DSM 100723T=CGMCC 4.7307T).

A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain 2Q3S-4-2T, was isolated from the surface-sterilized bark of Kandelia candel, collected from Cotai Ecological Zones in Macao, PR China. It was tested using a polyphasic approach to determine its taxonomic position. Strain 2Q3S-4-2T grew optimally without NaCl at 28-30 °C and at pH 7.0. Substrate mycelia and aerial mycelia were not formed and no diffusible pigments were observed on the media tested. Phylogenetic analysis, based on 16S rRNA gene sequences, suggested that strain 2Q3S-4-2T belonged to the genus Nakamurella, sharing highest 16S rRNA gene sequence similarity with Nakamurella flavida DS-52T (96.76 %). The DNA G+C content of strain 2Q3S-4-2T was 67.8 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid and MK-8(H4) was the predominant menaquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipids and phosphatidylinositol. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0. On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain 2Q3S-4-2T represents a novel species of the genus Nakamurella, for which the name Nakamurella endophytica sp. nov. is proposed. The type strain is 2Q3S-4-2T ( = DSM 100722T = CGMCC 4.7308T).