1. Light and Life in Baltimore—and Beyond
- Author
-
Michael Edidin
- Subjects
Microscope ,Light ,Confocal ,Biophysics ,History, 21st Century ,law.invention ,Mice ,Optics ,law ,Fluorescence Resonance Energy Transfer ,Animals ,Humans ,Fluorescent Dyes ,Fluorescence loss in photobleaching ,biology ,business.industry ,Chemistry ,Fluorescence recovery after photobleaching ,Congresses as Topic ,History, 20th Century ,Photobleaching ,Biophysical Review ,Förster resonance energy transfer ,Microscopy, Fluorescence ,Rhodopsin ,Cytoplasm ,Baltimore ,biology.protein ,business ,Biomarkers ,Fluorescence Recovery After Photobleaching - Abstract
Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.
- Published
- 2015