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Lifetime of Major Histocompatibility Complex Class-I Membrane Clusters Is Controlled by the Actin Cytoskeleton
- Source :
- Biophysical Journal. 102:1543-1550
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Lateral heterogeneity of cell membranes has been demonstrated in numerous studies showing anomalous diffusion of membrane proteins; it has been explained by models and experiments suggesting dynamic barriers to free diffusion, that temporarily confine membrane proteins into microscopic patches. This picture, however, comes short of explaining a steady-state patchy distribution of proteins, in face of the transient opening of the barriers. In our previous work we directly imaged persistent clusters of MHC-I, a type I transmembrane protein, and proposed a model of a dynamic equilibrium between proteins newly delivered to the cell surface by vesicle traffic, temporary confinement by dynamic barriers to lateral diffusion, and dispersion of the clusters by diffusion over the dynamic barriers. Our model predicted that the clusters are dynamic, appearing when an exocytic vesicle fuses with the plasma membrane and dispersing with a typical lifetime that depends on lateral diffusion and the dynamics of barriers. In a subsequent work, we showed this to be the case. Here we test another prediction of the model, and show that changing the stability of actin barriers to lateral diffusion changes cluster lifetimes. We also develop a model for the distribution of cluster lifetimes, consistent with the function of barriers to lateral diffusion in maintaining MHC-I clusters.
- Subjects :
- Models, Molecular
Phalloidine
Protein Stability
Anomalous diffusion
Chemistry
Vesicle
Membrane
Biophysics
Bridged Bicyclo Compounds, Heterocyclic
Actin cytoskeleton
Actin Cytoskeleton
Mice
Crystallography
Membrane protein
HLA Antigens
Animals
Thiazolidines
Diffusion (business)
Dynamic equilibrium
Exocytic vesicle
Subjects
Details
- ISSN :
- 00063495
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....e8c1fd43e7e9572a0c338b4cc753c3d4