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Rapid capping in alpha-spectrin-deficient MEL cells from mice afflicted with hereditary hemolytic anemia
- Source :
- The Journal of Cell Biology
- Publication Year :
- 1994
- Publisher :
- The Rockefeller University Press, 1994.
-
Abstract
- A spectrin-based membrane skeleton is important for the stability and organization of the erythrocyte. To study the role of spectrin in cells that possess complex cytoskeletons, we have generated alpha-spectrin-deficient erythroleukemia cell lines from sph/sph mice. These cells contain beta-spectrin, but lack alpha-spectrin as determined by immunoblot and Northern blot analyses. The effects of alpha-spectrin deficiency are apparent in the cells' irregular shape and fragility in culture. Capping of membrane glycoproteins by fluorescent lectin or antibodies occurs more rapidly in sph/sph than in wild-type erythroleukemia cells, and the caps appear more concentrated. The data support the idea that spectrin plays an important role in organizing membrane structure and limiting the lateral mobility of integral membrane glycoproteins in cells other than mature erythrocytes.
- Subjects :
- Time Factors
macromolecular substances
Mice
medicine
Animals
Spectrin
Immunologic Capping
Cytoskeleton
chemistry.chemical_classification
biology
Cell Membrane
H-2 Antigens
EPB41
Cell Biology
Articles
Virology
Mice, Mutant Strains
Cell biology
Friend murine leukemia virus
Red blood cell
Membrane glycoproteins
medicine.anatomical_structure
chemistry
Cell culture
biology.protein
Leukemia, Erythroblastic, Acute
Antibody
Glycoprotein
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 125
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....b515989eb2827a563a21ce6d3fc1741e