1. Beneficial Effect of Herbal Formulation KM1608 on Inflammatory Bowl Diseases: A Preliminary Experimental Study
- Author
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Bon Am Koo, Myoung-Sook Shin, Sang-Back Kim, Jimin Park, Gwi Seo Hwang, Ki Sung Kang, Han-Seok Choi, Jun Yeon Park, Sullim Lee, and Jaemin Lee
- Subjects
Terminalia chebula Retz ,Phytochemicals ,Anti-Inflammatory Agents ,Pharmaceutical Science ,Pharmacology ,030226 pharmacology & pharmacy ,01 natural sciences ,Analytical Chemistry ,Mice ,0302 clinical medicine ,Drug Discovery ,Phosphorylation ,Chromatography, High Pressure Liquid ,anti-inflammatory ,biology ,Dextran Sulfate ,Interleukin ,Aucklandia lappa DC ,Colitis ,Ulcerative colitis ,Terminalia chebula ,Chemistry (miscellaneous) ,Myeloperoxidase ,Molecular Medicine ,Cytokines ,Tumor necrosis factor alpha ,medicine.symptom ,Inflammation Mediators ,Oxidation-Reduction ,medicine.drug_class ,Inflammation ,Anti-inflammatory ,Article ,Zingiber officinale Roscoe ,lcsh:QD241-441 ,03 medical and health sciences ,lcsh:Organic chemistry ,medicine ,Animals ,Physical and Theoretical Chemistry ,ulcerative colitis ,010405 organic chemistry ,business.industry ,Plant Extracts ,Organic Chemistry ,medicine.disease ,Inflammatory Bowel Diseases ,0104 chemical sciences ,Disease Models, Animal ,RAW 264.7 Cells ,biology.protein ,business ,Proto-Oncogene Proteins c-akt - Abstract
Aucklandia lappa DC., Terminalia chebula Retz and Zingiber officinale Roscoe have been traditionally used in east Asia to treat chronic diarrhea and abdominal pain. This study aimed to evaluated the anti-inflammatory activity of KM1608, which is composed of three natural herbs in a mouse model of dextran sodium sulfate (DSS)-induced ulcerative colitis. The anti-inflammatory activity and underlying mechanism were assessed in vitro using LPS-treated RAW264.7 cells. The in vivo effect of KM1608 on DSS-induced colitis was examined after oral administration in mice. KM1608 significantly inhibited the inflammatory mediators such as nitric oxide, interleukin (IL)-6, monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor (TNF)-&alpha, in LPS-treated RAW264.7 cells. The inhibitory effect of KM1608 was attributed to the reduction of Akt phosphorylation in the LPS-treated cells. In the mouse model, oral administration of KM1608 significantly improved DSS-induced colitis symptoms, such as disease activity index (DAI), colon length, and colon weight, as well as suppressed the expression of IL-6, TNF-&alpha, and myeloperoxidase (MPO) in the DSS-induced colitis tissues. Taken together, KM1608 improved colitis through the regulation of inflammatory responses, suggesting that KM1608 has potential therapeutic use in the treatment of inflammatory diseases.
- Published
- 2018