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Preparation of Herbal Formulation for Inflammatory Bowel Disease Based on In Vitro Screening and In Vivo Evaluation in a Mouse Model of Experimental Colitis
- Source :
- Molecules, Volume 24, Issue 3, Molecules, Vol 24, Iss 3, p 464 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Many medicinal plants have been used traditionally in East Asia for the treatment of gastrointestinal disease and inflammation. The aim of this study was to evaluate the anti-inflammatory activity of 350 extracts (175 water extracts and 175 ethanol extracts) from 71 single plants, 97 mixtures of two plants, and seven formulations based on traditional medicine, to find herbal formulations to treat inflammatory bowel disease (IBD). In the in vitro screening, nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 levels were determined in LPS-treated RAW264.7 cells and the TNF-α induced monocyte-epithelial cell adhesion assay was used for the evaluation of the anti-inflammatory activity of the compounds. Dextran sulfate sodium (DSS)-induced colitis model and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis model were used to evaluate the therapeutic effect against IBD of the samples selected from the in vitro screening. KM1608, composed of Zingiber officinale, Terminalia chebula and Aucklandia lappa, was prepared based on the screening experiments. The oral administration of KM1608 significantly attenuated the severity of colitis symptoms, such as weight loss, diarrhea, and rectal bleeding, in TNBS-induced colitis. In addition, inflammatory mediators, such as myeloperoxidase, TNF-α, and IL-6 levels decreased in the lysate of colon tissues treated with KM1608. Collectively, KM1608 ameliorated colitis through the regulation of inflammatory responses within the colon, which indicated that KM1608 had potential for the treatment of IBD.
- Subjects :
- Zingiber officinale
Anti-Inflammatory Agents
Drug Evaluation, Preclinical
Pharmaceutical Science
Pharmacology
Inflammatory bowel disease
Monocytes
Analytical Chemistry
Aucklandia lappa
Mice
Terminalia chebula
0302 clinical medicine
Drug Discovery
anti-inflammatory activity
0303 health sciences
Dextran Sulfate
Interleukin
Colitis
Diarrhea
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Cytokines
Molecular Medicine
Female
Tumor necrosis factor alpha
medicine.symptom
Inflammation
Nitric Oxide
Article
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
inflammatory bowel disease
In vivo
medicine
Animals
Physical and Theoretical Chemistry
030304 developmental biology
Plant Extracts
Tumor Necrosis Factor-alpha
business.industry
Organic Chemistry
Epithelial Cells
Inflammatory Bowel Diseases
medicine.disease
Disease Models, Animal
RAW 264.7 Cells
Nitric Oxide Synthase
business
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....72418d7c42eeed8008fe5dd4109bb543