1. Guided bone regeneration with a gelatin layer and adenoviral delivery of c-myb enhances bone healing in rat tibia
- Author
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Jyoti Shrestha Takanche, Ji-Eun Kim, Jeong-Seok Kim, and Ho-Keun Yi
- Subjects
Bone mineral ,0303 health sciences ,Embryology ,Pathology ,medicine.medical_specialty ,food.ingredient ,Stromal cell ,Chemistry ,Bone Injury ,medicine.medical_treatment ,Biomedical Engineering ,030206 dentistry ,Bone healing ,Bone grafting ,Gelatin ,03 medical and health sciences ,0302 clinical medicine ,food ,medicine.anatomical_structure ,medicine ,Bone marrow ,Bone regeneration ,030304 developmental biology - Abstract
Aim: Bone healing becomes problematic during certain states, such as trauma. This study verifies whether the application of c-myb with gelatin promotes bone healing during bone injuries. Materials & methods: A biodegradable membrane was modified with adenoviral vector c-myb ( Ad/c-myb) and gelatin and applied in the bone injury site of rat tibia. Results: c-myb enhanced osteogenic differentiation and mineralization in bone marrow stromal cells after induction with osteogenic media. In vivo examination of rat tibia after application of the biodegradable membrane with Ad/c-myb and a gelatin layer demonstrated increased bone volume, bone mineral density, new bone formation and osteogenic molecules, compared with Ad/LacZ. Conclusion: c-myb has the potential to assist bone healing and may be applicable to the treatment of bone during injury.
- Published
- 2020