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Your search keyword '"S. Krüger-Krasagakes"' showing total 7 results
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7 results on '"S. Krüger-Krasagakes"'

1. Expression of interleukin 10 in human melanoma

Author

Tibor Diamantstein, Christoph Hüls, S. Krüger-Krasagakes, Claus Garbe, Thomas Blankenstein, Konstantin Krasagakis, and Edgar Schmitt

Subjects
Keratinocytes, Cancer Research, Pathology, medicine.medical_specialty, medicine.medical_treatment, Gene Expression, Biology, Immune system, Gene expression, Tumor Cells, Cultured, medicine, Humans, RNA, Messenger, RNA, Neoplasm, Neoplasm Metastasis, Melanoma, neoplasms, Skin, Messenger RNA, Biological activity, medicine.disease, Reverse transcriptase, Interleukin-10, Interleukin 10, Cytokine, Oncology, Cancer research, Research Article
Abstract

The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response. Images Figure 1 Figure 2

Published
1994

2. Expression of tumor necrosis factor by different tumor cell lines results either in tumor suppression or augmented metastasis

Author

S. Krüger-Krasagakes, Ulrich Kunzendorf, Zhihai Qin, Tibor Diamantstein, Hanno Hock, and Thomas Blankenstein

Subjects
Pathology, medicine.medical_specialty, Cancer Research, CD30, medicine.medical_treatment, Immunology, Molecular Sequence Data, Biology, Transfection, Metastasis, Mice, medicine, Tumor Cells, Cultured, Immunology and Allergy, Animals, Neoplasm Metastasis, Base Sequence, Tumor Necrosis Factor-alpha, Lymphoblast, Immunotherapy, Articles, Neoplasms, Experimental, medicine.disease, Cytokine, Cell culture, Mice, Inbred DBA, Cancer research, Tumor necrosis factor alpha, Cell Adhesion Molecules
Abstract

Tumor necrosis factor (TNF) produced by tumor cells after gene transfer can effectively suppress the growth of locally growing tumors. We wanted to test the effects of "local" TNF on the growth of a highly metastatic cell line. Therefore, a recombinant retrovirus allowing expression of the TNF gene by the beta-actin promotor has been constructed and used to infect the two tumor cell lines EB and ESB, which grow as solid tumor or metastasize, respectively. Expression of TNF by EB cells resulted in their rapid and dose-dependent rejection. In sharp contrast, mice injected with ESB cells producing similar amounts of TNF showed no signs of tumor suppression, but rather had reduced survival rates that correlated with enhanced hepatic metastases. The accelerated formation of liver metastases by ESB TNF cells could be reversed by an anti-TNF mAb. These results demonstrate the opposite effects TNF may have on tumor growth: suppression of a locally growing tumor and promotion of metastasis formation.

Published
1993

3. Supplement II: Abstracts of the international symposium on Skin Carcinogenesis in man and in experimental models. Heidelberg, 29–31 October 1991 (pp S61–S88)

Author

C. J. Kemp, G. Stingl, C. Caulín, E. G. Jung, H. Tanooka, J. Lassus, E. F. Griffin, Douglas R. Lowy, J. L. Jorcano, J. C. Wang, L. Weber, R. Kato, Paul Janiaud, S. Ohno, A. Schwaaf, R. Gollhausen, N. Sönnichsen, H. Hug, Toshio Kuroki, M. Yaar, J. R. Schlehofer, K. Krasagakis, PE Purkis, Monika M. Gross, H. Heine, H. Mukhtar, J. A. Newton, G. Reisbach, C. Bauer, A. Winter, K. M. Niemi, S. Yamamoto, Bernd L. Sorg, V. A. DeLeo, S. Bruvers, P. Navarro, A. Ootsuyama, G. Tadini, Bert J. Vermeer, D. English, A. B. Bianchi, S. Feil, A. Lehmus, H. Winter, P. T. Strickland, C. Proby, J. M. Foidart, R. Eckert, R. E. Albert, N. E. Fusenig, E. Lee, R. D. Granstein, P. Bums, E. Berti, J. Jürgensmeier, H. Roeser, J. Nährig, A. Anders, F. R. de Gruijl, C. S. Baxter, R. Mailhammer, H. van Weelden, Y. Fujiwara, E. Filvaroff, E. Weber, S. Froschermaier, G. Graf, J. C. Barrett, J. Weiss, H. Weber, B. Hennig, M. Miller, F. Urbach, K. Yamamura, E. Pâques, A. Hülsen, Seymour Garte, B. A. Gilchrest, S. Neill, K. Thalmeier, C. Zechel, Jan P. Vandenbroucke, B. Epe, P. Höfler, B. Przybilla, A. Markey, C. Gilles, C. Bauluz, I. B. Weinstein, U. Van der Piepen, Fokko J. Van Der Woude, T. Jimbo, A. Cano, P. Tomakidi, M. Quintanilla, A. Real, T. Grande, G. T. Bowden, H. Friesel, Y. Mishima, Jan N. Bouwes Bavinck, D. Breitkreutz, Stanley J. Miller, M. Piette, E. Wagner, M. Buček, A. Kopp-Schneider, C. A. Afshari, A. Ranki, M. Garmyn, Margaret L. Kripke, C. Baxter, E. Hecker, Hiroshi Tanooka, F. Harks, E. Lopez-Bran, P. A. Futreal, H. Wei, M. B. Abdel-Naser, A. Diugosz, S. Altmeier, J. Macejewski, Uwe Wollina, J. Römisch, B. Eberlein, E. B. Broecker, Y. Funasaka, M. Glover, M. Haas, S. Gruner, T. Bishop, J. Leers, G. Picht, A. Gilani, W. Diezel, D. S. Silvers, A. Glick, R. Krauß, H. Harris, Anne Østerlind, J. Levy, A. Cerri, E. Danen, K. Schiess, E. Viesel, H. Gröger, B. C. Bastian, K. Hayashibe, K. H. Richter, K. Frenkel, Odilia Popanda, M. Gómez, I. Moll, U. Schleenbecker, M. Ueda, Fritz Anders, H. D. Volk, K. Möller, M. Ichihashi, M. Martín, G. Krauter, S. Krüger-Krasagakes, D. J. Ruiter, J. C. van der Leun, M. Götschl, R. Niedner, Sylvia A. Sedman, T. M. Rünqer, Akira Ootsuyama, Judith P. Johnson, A. Montes, A. G. Ushmorov, G. Bauer, R. Schnapke, S. Kahn, B. Kempkes, C. Garbe, B. Steinbauer, B. K. Armstrong, P. Plein, T. Schneider, C. Missero, B. Schlatterer, M. Schara, P. J. Heenan, M. Stephan, B. A. Burkhart, A. J. P. Klein-Szanto, Eva-B. Bröcker, R. Halaban, S. Grabbe, G. N. P. van Muijen, E. Azizi, D. Schaefer, A. A. Hartmann, C. Ballestin, P. Klein-Bauernschmitt, R. Shukla, G. Kelfkens, M. Nelson, Friedrich Rippmann, M. Kaszkin, S. G. Zubova, Bruce D. Cohen, T. Cody, A. Kricker, V. B. Okulov, P. Fuchs, V. Kinzel, S. Osada, A. Balmain, A. B. Stoler, T. T. Sun, J. Svetek, W. D. Lehmann, F. Larcher, P. Krieg, Jürgen Schweizer, M. Hergenhahn, A. Faissner, G. P. Dotto, C. J. Conti, U. Burcin, L. Hültner, V. Bataille, G. Fürstenberger, EB Lane, A. Smith, D. Jahrens, K. Elgjo, Walter Troll, A. Gandarillas, M. Schön, R. D. Owen, S. Ramón y Cajal, Heinz Walter Thielmann, A. O. Danilov, S. H. Yuspa, J. Cuzick, P. L. Randell, Sylvia Unger, J. A. Boyd, C. Sutter, N. M. Navone, IM Leigh, H. J. Stark, L. A. Annab, R. Gitto, James M. Spencer, C. E. Orfanos, R. M. Lavker, W. Tilgen, R. Albert, H. L. Moses, Eric J. Stanbridge, R. Kosters, Rainer Schmidt, P. Boukamp, E. Schöpf, U. Pascheberg, Yuichi Hashimoto, A. Robledo, F. Marks, J. Sherman, J. Richards, C. E. Klein, Frans H.J. Claas, S. Pečar, Bernard M. Mechler, Doris Rueß, B. Fiebich, Lutz Edler, John T. Schiller, and H. Fujiki

Subjects
Oncology, Cancer Research, medicine.medical_specialty, business.industry, Internal medicine, Medicine, General Medicine, business, Carcinogenesis, medicine.disease_cause
Published
1991

4. Production of Cytokines by Human Melanoma Cells and Melanocytes

Author

S. Krüger-Krasagakes, Claus Garbe, T. Diamantstein, and Konstantin Krasagakis

Subjects
Cytokine, Immune system, Effector, Melanoma, medicine.medical_treatment, medicine, Cancer research, Tumor necrosis factor alpha, Biology, medicine.disease, In vitro, Reverse transcriptase, Metastasis
Abstract

Experimental animal models have shown that various cytokines, depending on their specific properties, may support growth and metastasis of tumor cells or even lead to tumor rejection. The analysis of expression of cytokine genes by melanoma cell lines indicated that melanoma cells constitutively produce both autostimulatory and inhibitory cytokines. Using reverse transcriptase polymerase chain reaction analysis, simultaneous expression of several cytokines, including interleukin-1 beta (IL-1β), IL-6, IL-8, tumor necrosis factor-α, and granulocyte-macrophage colony-stimulating factor, by melanoma cells was found. The same cytokine transcripts were detected in melanocytes, suggesting that cells of the melanocytic lineage express a specific pattern of cytokines in vitro. All these cytokines are known to be able to stimulate effector cells of the host. Additionally, production of mRNA for IL-10, a cytokine with potential immunosuppressive properties, was detected in melanoma cells and melanocytes. These and other cytokines are likely to be involved in the immune response to cancer and at this time it is unknown what the net effects of multiple cytokines are on the outcome of the host response to tumor.

Published
1995

5. Eosinophils infiltrating interleukin-5 gene-transfected tumors do not suppress tumor growth

Author

Günther Richter, Weiqun Li, Thomas Blankenstein, Tibor Diamantstein, and S. Krüger-Krasagakes

Subjects
Adoptive cell transfer, medicine.medical_treatment, Immunology, Molecular Sequence Data, Biology, medicine.disease_cause, Transfection, Polymerase Chain Reaction, Mice, Immune system, medicine, Immunology and Allergy, Animals, Interleukin 5, Immunity, Cellular, Mice, Inbred BALB C, Base Sequence, Neoplasms, Experimental, Eosinophil, medicine.disease, Eosinophils, medicine.anatomical_structure, Cytokine, Oligodeoxyribonucleotides, Cancer research, Plasmacytoma, Female, Interleukin-5, Carcinogenesis
Abstract

Tumor-associated eosinophils have been observed in human tumors and in experimental tumor models, but their function is poorly understood. To study the role of eosinophils during tumor growth, the plasmacytoma J558L and the mammary adenocarcinoma TS/A were transfected with an expression vector encoding the murine gene for interleukin-5 (IL-5), a cytokine inducing proliferation and activation of eosinophils. Injection of parental cells, mock-transfectants and IL-5-producing cells into syngeneic mice showed that local IL-5 secretion induced rapid tumor infiltration by eosinophils, as evidenced by immunohistochemical staining, but nevertheless did not alter the tumor growth kinetics of IL-5 transfectants. Therefore, the mere presence of IL-5 and eosinophils was not sufficient to induce a protective host immune response.

Published
1993

6. 46 Cytokine network regulating melanoma cell growth in vitro

Author

Ch.C. Zouboulis, C. E. Orfanos, Konstantin Krasagakis, and S. Krüger-Krasagakes

Subjects
Cancer Research, Oncology, Cell growth, Cytokine Network, Melanoma, medicine, Cancer research, Dermatology, Biology, medicine.disease, In vitro
Published
1997

7. Production of cytokines by melanoma cells

Author

C. Garbe, T. Diamanstein, Konstantin Krasagakis, and S. Krüger-Krasagakes

Subjects
Cancer Research, Oncology, Melanoma, medicine, Cancer research, Production (economics), Dermatology, Biology, medicine.disease
Published
1993

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