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Expression of interleukin 10 in human melanoma
- Source :
- British Journal of Cancer
- Publication Year :
- 1994
- Publisher :
- Springer Science and Business Media LLC, 1994.
-
Abstract
- The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response. Images Figure 1 Figure 2
- Subjects :
- Keratinocytes
Cancer Research
Pathology
medicine.medical_specialty
medicine.medical_treatment
Gene Expression
Biology
Immune system
Gene expression
Tumor Cells, Cultured
medicine
Humans
RNA, Messenger
RNA, Neoplasm
Neoplasm Metastasis
Melanoma
neoplasms
Skin
Messenger RNA
Biological activity
medicine.disease
Reverse transcriptase
Interleukin-10
Interleukin 10
Cytokine
Oncology
Cancer research
Research Article
Subjects
Details
- ISSN :
- 15321827 and 00070920
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....7f3a2a09992b1fe7143aea6e2aed1905