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126 results on '"Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL)"'

1. Association between thermal responses, medical events, performance, heat acclimation and health status in male and female elite athletes during the 2019 Doha World Athletics Championships

Author: Sebastien Racinais, George Havenith, Polly Aylwin, Mohammed Ihsan, Lee Taylor, Paolo Emilio Adami, Maria-Carmen Adamuz, Marine Alhammoud, Juan Manuel Alonso, Nicolas Bouscaren, Sebastian Buitrago, Marco Cardinale, Nicol van Dyk, Chris J Esh, Josu Gomez-Ezeiza, Frederic Garrandes, Louis Holtzhausen, Mariem Labidi, Gűnter Lange, Alexander Lloyd, Sebastien Moussay, Khouloud Mtibaa, Nathan Townsend, Mathew G Wilson, Stephane Bermon, ASPETAR Orthopaedic and Sports Medicine Hospital [Qatar], Loughborough University, National University of Singapore (NUS), University of Technology Sydney (UTS), Laboratoire Motricité Humaine Expertise Sport Santé (LAMHESS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université de Toulon (UTLN)-Université Côte d'Azur (UCA), Centre d'Investigation Clinique de La Réunion - INSERM (CIC 1410), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), University College of London [London] (UCL), School of Sport, Exercise & Health Sciences, Stellenbosch University, University of Pretoria [South Africa], Mobilités : Vieillissement, Pathologie, Santé (COMETE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), GIP Cyceron (Cyceron), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Qatar University, Hamad Bin Khalifa University (HBKU), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, exercise, Acclimatization, Health Status, [SDV]Life Sciences [q-bio], [SCCO.NEUR]Cognitive science/Neuroscience, hot temperature, [SHS.PSY]Humanities and Social Sciences/Psychology, Physical Therapy, Sports Therapy and Rehabilitation, Walking, General Medicine, Athletes, Humans, Female, Orthopedics and Sports Medicine, Body Temperature Regulation, Retrospective Studies
Abstract: PurposeTo determine associations between thermal responses, medical events, performance, heat acclimation and health status during a World Athletics Championships in hot-humid conditions.MethodsFrom 305 marathon and race-walk starters, 83 completed a preparticipation questionnaire on health and acclimation. Core (Tcore; ingestible pill) and skin (Tskin; thermal camera) temperatures were measured in-competition in 56 and 107 athletes, respectively. 70 in-race medical events were analysed retrospectively. Performance (% personal best) and did not finish (DNF) were extracted from official results.ResultsPeak Tcore during competition reached 39.6°C±0.6°C (maximum 41.1°C). Tskin decreased from 32.2°C±1.3°C to 31.0°C±1.4°C during the races (pcore was not related to DNF (25% of starters) or medical events (p≥0.150), whereas Tskin, Tskin rate of decrease and Tcore-to-Tskin gradient were (p≤0.029). A third of the athletes reported symptoms in the 10 days preceding the event, mainly insomnia, diarrhoea and stomach pain, with diarrhoea (9% of athletes) increasing the risk of in-race medical events (71% vs 17%, pcore (39.4°C±0.4°C vs 39.8°C±0.7°C, p=0.044) and larger in-race decrease in Tskin (−1.4°C±1.0°C vs −0.9°C±1.2°C, p=0.060), than non-acclimated athletes. Although not significant, they also showed lower DNF (19% vs 30%, p=0.273) and medical events (19% vs 32%, p=0.179).ConclusionTskin, Tskin rate of decrease and Tcore-to-Tskin gradient were important indicators of heat tolerance. While heat-acclimated athletes ranked better, recent diarrhoea represented a significant risk factor for DNF and in-race medical events.
Published: 2022

2. Skeletal Ryanodine Receptors Are Involved in Impaired Myogenic Differentiation in Duchenne Muscular Dystrophy Patients

Author: Jean-Marie Cuisset, Jérôme Cottalorda, Stefan Matecki, Gérald Hugon, Alain Lacampagne, Pierre Meyer, Cécile Notarnicola, Hugues Chevassus, Albano C. Meli, Pascal Sabouraud, François Rivier, Léonard Féasson, Mirna Khalil, Gilles Carnac, Jérémy Salvador, Claude Cances, Isabelle Desguerre, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre d'investigation clinique de Toulouse (CIC 1436), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Cité (UPC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre Hospitalier Universitaire de Reims (CHU Reims), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Pôle Santé publique et médecine publique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Paris Cité (UPCité), Herrada, Anthony, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Université de Paris (UP)
Subjects: Male, Duchenne muscular dystrophy, Muscle Fibers, Skeletal, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Muscle Development, Calcium in biology, Dystrophin, Myoblasts, 0302 clinical medicine, Myocyte, Biology (General), Child, Spectroscopy, Cells, Cultured, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Ryanodine receptor, Myogenesis, Cell Differentiation, General Medicine, musculoskeletal system, Computer Science Applications, Chemistry, Sarcoplasmic Reticulum, medicine.anatomical_structure, Child, Preschool, myogenesis, tissues, Intracellular, musculoskeletal diseases, medicine.medical_specialty, congenital, hereditary, and neonatal diseases and abnormalities, QH301-705.5, Catalysis, Article, Inorganic Chemistry, Tacrolimus Binding Proteins, 03 medical and health sciences, ryanodine receptor, endomysial fibrosis, human, Internal medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine, Humans, Calcium Signaling, Physical and Theoretical Chemistry, Muscle, Skeletal, Molecular Biology, QD1-999, 030304 developmental biology, RYR1, business.industry, Organic Chemistry, Skeletal muscle, Ryanodine Receptor Calcium Release Channel, medicine.disease, Muscular Dystrophy, Duchenne, Endocrinology, Calcium, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030217 neurology & neurosurgery
Abstract: Duchenne muscular dystrophy (DMD) is characterized by progressive muscle wasting following repeated muscle damage and inadequate regeneration. Impaired myogenesis and differentiation play a major role in DMD as well as intracellular calcium (Ca2+) mishandling. Ca2+ release from the sarcoplasmic reticulum is mostly mediated by the type 1 ryanodine receptor (RYR1) that is required for skeletal muscle differentiation in animals. The study objective was to determine whether altered RYR1-mediated Ca2+ release contributes to myogenic differentiation impairment in DMD patients. The comparison of primary cultured myoblasts from six boys with DMD and five healthy controls highlighted delayed myoblast differentiation in DMD. Silencing RYR1 expression using specific si-RNA in a healthy control induced a similar delayed differentiation. In DMD myotubes, resting intracellular Ca2+ concentration was increased, but RYR1-mediated Ca2+ release was not changed compared with control myotubes. Incubation with the RYR-calstabin interaction stabilizer S107 decreased resting Ca2+ concentration in DMD myotubes to control values and improved calstabin1 binding to the RYR1 complex. S107 also improved myogenic differentiation in DMD. Furthermore, intracellular Ca2+ concentration was correlated with endomysial fibrosis, which is the only myopathologic parameter associated with poor motor outcome in patients with DMD. This suggested a potential relationship between RYR1 dysfunction and motor impairment. Our study highlights RYR1-mediated Ca2+ leakage in human DMD myotubes and its key role in myogenic differentiation impairment. RYR1 stabilization may be an interesting adjunctive therapeutic strategy in DMD.
Published: 2021

3. Left ventricular dyssynchrony and post-systolic shortening in young bodybuilders using anabolic-androgenic steroids

Author: Iris Schuster, Thomas Rupp, Antoine Grandperrin, Omar Izem, Stéphane Nottin, Philippe Obert, EA4278 Laboratoire de Pharm-Ecologie Cardiovasculaire (LaPEC), Avignon Université (AU), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), MORNET, Dominique, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, medicine.medical_specialty, Adolescent, Physiology, Cardiac fibrosis, [SDV]Life Sciences [q-bio], Heart Ventricles, Diastole, Context (language use), 030204 cardiovascular system & hematology, Ventricular Function, Left, 03 medical and health sciences, Basal (phylogenetics), 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Physiology (medical), Internal medicine, Medicine, Humans, Mass index, 030212 general & internal medicine, Ventricular dyssynchrony, Exercise, Testosterone Congeners, Anabolic-androgenic steroids, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Ejection fraction, biology, Ventricular Remodeling, business.industry, Athletes, Post-systolic shortening, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, 16. Peace & justice, biology.organism_classification, medicine.disease, Myocardial Contraction, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Dyssynchrony, Cardiac hypertrophy, Cardiology, Cardiology and Cardiovascular Medicine, business
Abstract: International audience; Background: Left ventricular (LV) remodeling, characterized by increased LV hypertrophy and depressed function, is observed in strength-trained athletes who use anabolic-androgenic steroids (AAS). Previous studies reported an increase in cardiac fibrosis in these athletes, which could promote intraventricular dyssynchrony. In this context, this study evaluated LV dyssynchrony in strength-trained athletes using AA; hypothesizing that the use of AAS leads to an increase in LV dyssynchrony with an increase in post-systolic shortening.Methods: Forty-four participants (aged 20-40 years) were divided into three age-matched groups: strength-trained athletes using AAS (users, n=14) and those who were not (non-users, n=15), and healthy sedentary men (controls, n=15),. After completing a survey, each participant was assessed with 2D-strain echocardiography. Left ventricular dyssynchrony was quantified using the standard deviation of the time to peak for longitudinal strains (SD), the longitudinal strain delay index (LSDI) and the segmental post-systolic index (PSI).Results: Users exhibited a greater LV mass index and higher systolic and diastolic functions than both controls and non-users. The decrease in LV strains in users was predominantly observed at the interventricular segments. The SD, LSDI and PSI, calculated on the basal inferoseptal, basal anteroseptal and basal inferolateral segments, were higher in users.Conclusion: The results strongly support that the specific LV remodeling observed in young AAS users was associated with an increase in LV dyssynchrony. The correlations with ejection fraction suggested that wasted energy, due to post-systolic shortenings, contributed in part to the decrease in LV function in strength-trained athletes using AAS.
Published: 2021

4. Association between physical activity and sedentary behaviour on carotid atherosclerotic plaques: an epidemiological and histological study in 90 asymptomatic patients

Author: Antoine Millon, Mathilde Mura, Philippe Connes, Stéphanie Chanon, Nellie Della-Schiava, Vincent Pialoux, Patrick Lermusiaux, Aurélie Vieille-Marchiset, Pauline Mury, Virginie Nicaise, Diane Collet-Benzaquen, Erica N. Chirico, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire sur les Vulnérabilités et l'Innovation dans le Sport (EA 7428) (L-VIS), Université de Lyon-Université de Lyon, Laboratoire Technipath, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Hôpital Edouard Herriot [CHU - HCL], Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Claude Bernard Lyon 1 (UCBL), Cooper Medical School of Rowan University [Camden] (CMSRU), Institut Universitaire de France, and Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, [SDV]Life Sciences [q-bio], medicine.medical_treatment, physical activity, Carotid endarterectomy, 030204 cardiovascular system & hematology, Metabolic equivalent, [SCCO]Cognitive science, 0302 clinical medicine, Risk Factors, Medicine, Orthopedics and Sports Medicine, 030212 general & internal medicine, Stroke, ComputingMilieux_MISCELLANEOUS, exercise physiology, Endarterectomy, Aged, 80 and over, Endarterectomy, Carotid, education.field_of_study, biology, cardiovascular, Fibrous cap, General Medicine, Plaque, Atherosclerotic, Carotid Arteries, medicine.anatomical_structure, Cardiovascular Diseases, Cardiology, Female, medicine.symptom, medicine.medical_specialty, Population, potential conflict of interest, Hemorrhage, Physical Therapy, Sports Therapy and Rehabilitation, Asymptomatic, lipids, 03 medical and health sciences, Internal medicine, absence of any commercial or financial relationships that could be construed as a, Humans, education, Exercise, Aged, business.industry, medicine.disease, Cross-Sectional Studies, Asymptomatic Diseases, Histopathology, Sedentary Behavior, business
Abstract: ObjectiveCarotid atherosclerotic plaques are a source of emboli for stroke. ‘Unstable’ carotid atherosclerotic plaques may have intraplaque haemorrhages, neovessels, prevalent macrophages, excessive calcium deposits, a large lipid core and a thin fibrous cap. Regular physical activity (PA) may lower the risk of plaques becoming unstable. We evaluated the association of both PA and sedentary behaviour (SB) with carotid plaque histopathology.Methods90 asymptomatic patients who were undergoing carotid endarterectomy for carotid artery narrowing identified on ultrasound reported their PA and SB by questionnaires. We calculated PA intensity in MET (metabolic equivalent of task)-min/week. For analysis, the population was divided into tertiles according to PA (T1PA: the less PA patients; T2PA: the intermediate PA patients; T3PA: the most physically active patients) (T1PAResultsIntraplaque haemorrhage was less frequent in the most physically active tertile (T3PA, 48%) versus T1PA (74%) and in the least sedentary tertile T1SB (50%) versus T3SB (71%). The intraplaque haemorrhage was less frequent in those who exercised more than 900 MET-min/week (59% vs 47% for >900 and ConclusionIn this cross-sectional study of asymptomatic patients who underwent endarterectomy (i) higher reported PA, (ii) intensity of PA and (iii) lower reported SB were associated with lower prevalence of intraplaque haemorrhage. This could be a mechanism whereby PA protects against cerebrovascular disease (stroke) and death.
Published: 2019

5. Active versus local vibration warm-up effects on knee extensors stiffness and neuromuscular performance of healthy young males

Author: Darryl J. Cochrane, Thibault Besson, Alessandro Zambelli, Robin Souron, Loïc Espeit, Thomas Lapole, Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), Université de Nantes (UN), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, medicine.medical_specialty, Warm-Up Exercise, Vastus medialis, [SDV]Life Sciences [q-bio], Physical Therapy, Sports Therapy and Rehabilitation, Electromyography, Athletic Performance, Vibration, Quadriceps Muscle, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Isometric Contraction, medicine, Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, Passive stiffness, ComputingMilieux_MISCELLANEOUS, Young male, Cross-Over Studies, Knee extensors, medicine.diagnostic_test, business.industry, Stiffness, 030229 sport sciences, Muscle stiffness, musculoskeletal system, Crossover study, Elasticity, medicine.symptom, business
Abstract: To compare the effects of local-vibration and active warm-up on knee extensors muscle stiffness and neuromuscular performance.Experimental crossover study.Thirteen participants performed three 15-min warm-up protocols of control (CON), active (ACT) and local-vibration (LV) in separate testing session. Passive stiffness of vastus lateralis (VL) and vastus medialis (VM) by shear wave elastography and neuromuscular performance were assessed before and 2-min after each condition.A decrease in muscle stiffness was reported after ACT for VL (-16.0±6.6%; p0.001) and VM (-10.2±8.7%; p=0.03) while no changes were reported after CON (p=0.46 and p=0.34 for VL and VM, respectively) and LV (p=0.07 and p=0.46 for VL and VM, respectively). Maximal jump performances increased during squat (+8.5±6.6%; p0.001) and countermovement jump (+5.2±5.8%; p0.001) after ACT while no changes were reported after CON and LV during squat (p=0.16 and p=0.81, respectively) and countermovement jump (p=0.18 and p=0.31, respectively). We further report that each condition was ineffective to inducing changes in maximal voluntary isometric contraction force (p=0.18), rate of force development (p=0.92), twitch parameters (p0.05) as well as central modulations as reported by the unchanged voluntary activation level (p=0.24) and maximal electromyography (EMG) recorded from the VL (p=0.44).The active warm-up acutely reduced muscle stiffness and increased muscle performance during maximal dynamic tasks. With regard to LV, further studies are required to determine optimal parameters (frequency, amplitude, duration) to significantly increase muscle performance.
Published: 2019

6. Determining the change in length of the anterolateral ligament during knee motion: A three-dimensional optoelectronic analysis

Author: Sven Putnis, Rémi Philippot, Frédéric Farizon, Thomas Neri, Samuel Grasso, Rodolphe Testa, Loic Laurendon, Margaux Dehon, David A. Parker, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Univ Lyon – UJM-Saint-Etienne, Inter-university Laboratory of Human Movement Science, EA 7424, Université Jean Monnet [Saint-Étienne] (UJM), Department of Computer Science [Hull], University of Hull, Centre d'Orthopédie et Traumatologie (COT), and CHU Saint-Etienne
Subjects: Male, musculoskeletal diseases, Anterolateral ligament, Knee Joint, Rotation, Intraclass correlation, [SDV]Life Sciences [q-bio], Biophysics, Kinematics, 03 medical and health sciences, Imaging, Three-Dimensional, 0302 clinical medicine, Cadaver, medicine, Humans, Orthopedics and Sports Medicine, Range of Motion, Articular, ComputingMilieux_MISCELLANEOUS, Reliability (statistics), Mathematics, Reproducibility, Ligaments, business.industry, Reproducibility of Results, 030229 sport sciences, Middle Aged, musculoskeletal system, Biomechanical Phenomena, medicine.anatomical_structure, Optoelectronics, Female, business, Cadaveric spasm, 030217 neurology & neurosurgery
Abstract: Background The variation of the anterolateral ligament (ALL) length during knee motion is still unclear, and the knee position in which a reconstruction graft should be tensioned remains controversial. The objective of this study was to determine the variation of the ALL length during knee motion using a three-dimensional optoelectronic system. Methods Kinematic analyses of 20 cadaveric knees were performed using a Motion Analysis® system. The variability of the measurements made during the five acquisition cycles was studied. Reliability was evaluated by two separate measurement sessions, with complete system reinstallation, using different cadavers and a new operator. The ALL length was analysed from extension to full flexion in three rotational conditions. Findings When analysing the reliability of the five cycles, 82% of the measurements we found to have an Intra Class Correlation (ICC) >0.85. The reproducibility of inter-sessional measures by different operators and different cadavers was either good (ICC >0.75) or excellent (ICC >0.85). The ALL length was maximum in full internal rotation with the knee at 25° of flexion. Interpretation This three-dimensional optoelectronic protocol allowed us to analyse the variation of the ALL length during intact knee motion with good reliability and the required accuracy to analyse this variable. The maximal length and highest tension of the ALL was reported at 25° of knee flexion in internal rotation, suggesting this as the optimal position for the knee joint when tensioning an ALL reconstruction.
Published: 2019

7. Hydroxyurea therapy modulates sickle cell anemia red blood cell physiology: Impact on RBC deformability, oxidative stress, nitrite levels and nitric oxide synthase signalling pathway

Author: Elie Nader, Marc Romana, Nicolas Guillot, Philippe Connes, Cyril Martin, Céline Renoux, Philippe Joly, Romain Fort, Marijke Grau, Bianca Collins, Alexandra Gauthier, Solène Poutrel, Giovanna Cannas, Katja Walpurgis, Wilhelm Bloch, Arnaud Hot, Mario Thevis, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Service de Médecine Interne, Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Center for Preventive Doping Research, Institut de recherche en informatique de Toulouse (IRIT), Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Pharmacogénétique et abords thérapeutiques des maladies héréditaires, Université des Antilles et de la Guyane (UAG)-Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
Subjects: Adult, Male, Nitroprusside, 0301 basic medicine, Cancer Research, Erythrocytes, Physiology, Clinical Biochemistry, Anemia, Sickle Cell, Nitric Oxide, medicine.disease_cause, Biochemistry, Nitric oxide, 03 medical and health sciences, chemistry.chemical_compound, Erythrocyte Deformability, medicine, Humans, Hydroxyurea, Phosphorylation, Nitrites, chemistry.chemical_classification, Reactive oxygen species, biology, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, hemic and immune systems, medicine.disease, Sickle cell anemia, 3. Good health, Nitric oxide synthase, Oxidative Stress, Red blood cell, 030104 developmental biology, medicine.anatomical_structure, chemistry, biology.protein, Female, Sodium nitroprusside, Hemoglobin, Nitric Oxide Synthase, Reactive Oxygen Species, Proto-Oncogene Proteins c-akt, Oxidative stress, Signal Transduction, circulatory and respiratory physiology, medicine.drug
Abstract: International audience; Hydroxyurea (HU) has been suggested to act as a nitric oxide (NO) donor in sickle cell anemia (SCA). However, little is known about the HU NO-related effects on red blood cell (RBC) physiology and NO signalling pathway. Thirty-four patients with SCA (22 under HU treatment (HU+) and 12 without (HU-)) and 17 healthy subjects (AA) were included. RBC nitrite content, deformability and reactive oxygen species (ROS) levels were measured. RBC NO-synthase (RBC-NOS) signalling pathway was assessed by the measurement of RBC-NOS serine 1177 and RBC-AKT serine 473 phosphorylation. We also investigated the in vitro effects of Sodium Nitroprusside (SNP), a NO donor, on the same parameters in SCA RBC. RBC nitrite content was higher in HU+ than in HU-and AA. RBC deformability was decreased in SCA patients compared to AA but the decrease was more pronounced in HU-. RBC ROS level was increased in SCA compared to AA but the level was higher in HU-than in HU+. RBC-NOS serine 1177 and RBC-AKT serine 473 phosphorylation were decreased in HU+ compared to HU-and AA. SCA RBC treated with SNP showed increased deformability, reduced ROS content and a decrease in AKT and RBC-NOS phosphorylation. Our study suggests that HU, through its effects on foetal hemoglobin and possibly on NO delivery, would modulate RBC NO signalling pathway, RBC rheology and oxidative stress.
Published: 2018

8. Pedobarographic Statistical Parametric Mapping may identify specific plantar pressure patterns in patients with diabetes mellitus among different degrees of peripheral neuropathy: A pilot study

Author: Giorgina Barbara Piccoli, Jean-Michel Audebrand, Baptiste Morel, Antoine Chatrenet, Bruno Beaune, Massimo Torreggiani, Motricité, interactions, performance EA 4334 / Movement - Interactions - Performance (MIP), Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Le Mans Université (UM), Centre Hospitalier Le Mans (CH Le Mans), Service Néphrologie [CH Le Mans], Department of Biological and Clinical Sciences, University of Turin, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Le Mans Université (UM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), Université de Nantes (UN)-Université de Nantes (UN), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, [SDV.CAN]Life Sciences [q-bio]/Cancer, Pilot Projects, Walking, Statistical parametric mapping, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Physical medicine and rehabilitation, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Diabetic Neuropathies, Diabetes mellitus, Internal Medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Diabetes Mellitus, Pressure, Medicine, Humans, In patient, 030212 general & internal medicine, Lead (electronics), Pedobarography, ComputingMilieux_MISCELLANEOUS, Aged, business.industry, Foot, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Middle Aged, medicine.disease, Gait, Peripheral neuropathy, Case-Control Studies, Female, Complication, business, Gait Analysis
Abstract: Aims Peripheral neuropathy (PN) in patients with diabetes can lead to changes in the distribution of plantar pressure during walking, which can be recorded with pedobarography. Compared to traditional spatial data reduction analysis, the pedobarographic Statistical Parametric Mapping (pSPM) allows comparison of the footprints with the advantage that sub-regions do not need to be defined a priori. Aim of the study was to test the potential of pSPM in identifying specific distribution of spatial pressure in different stages of PN. Methods PN was defined according to usual tools (i.e., tendon reflexes and sensory tests). Four groups were compared: patients with diabetes without PN (n = 24; 239 steps); with signs of mild PN (n = 12; 117 steps); with signs of severe PN (n = 6; 52 steps) and a control group without diabetes (n = 12; 124 steps). Traditional spatial data reduction and pSPM were performed to compare plantar pressures in the different groups. Results In patients with PN, traditional spatial data reduction analysis showed lower plantar pressures with PN severity. pSPM analysis is able to better define the initial changes: mild PN patients presents higher pressures on the anterior side of the metatarsal heads compared to patients without neuropathy. Patients with severe PN are characterised by higher pressures under the medial foot arch compared to other groups. Conclusions pSPM may identify specific features of plantar pressure distribution during walking in patients with mild PN and may become a useful screening tool for a timely identification of this complication.
Published: 2021

9. Lower limb muscle injury location shift from posterior lower leg to hamstring muscles with increasing discipline-related running velocity in international athletics championships

Author: Laurent Navarro, Jean-Benoit Morin, Christine Hanon, Lilian Lacourpaille, Pedro Branco, Antonio J. Morales-Artacho, Karsten Hollander, Astrid Junge, Gaël Guilhem, Pascal Edouard, Sébastien Le Garrec, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT), Motricité, interactions, performance EA 4334 / Movement - Interactions - Performance (MIP), Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Le Mans Université (UM), French Institute of Sport (INSEP), Research Department, Laboratory Sport, Expertise and Performance (EA7370) (SEP (EA7370)), Institut national du sport, de l'expertise et de la performance (INSEP), Fédération Française d'Athlétisme (FFA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), French Institute of Sport (INSEP), Pôle Médical, Centre Européen de Réalité Virtuelle (CERV), École Nationale d'Ingénieurs de Brest (ENIB), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, medicine.medical_specialty, Hamstring muscles, Competitive Behavior, Sports injury, Epidemiology, Physical Therapy, Sports Therapy and Rehabilitation, Context (language use), Hamstring Muscles, Marathon Running, Injury surveillance, Track and field, Running, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Lower limb muscle, medicine, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, Prospective Studies, Sex Distribution, Top-level athletes, Track and field athletics, Muscle, Skeletal, Long distance runners, biology, Athletes, business.industry, Incidence, 030229 sport sciences, biology.organism_classification, Muscle injury risk, Female, Injury location, business, human activities, Hamstring, Leg Injuries
Abstract: International audience; Objective: To analyse the rates of lower limb muscle injuries in athletics disciplines requiring different running velocities during international athletics championships.Design: Prospective total population study.Methods: During 13 international athletics championships (2009–2019) national medical teams and local organizing committee physicians daily reported all newly incurred injuries using the same study design, injury definition and data collection procedures. In-competition lower limb muscle injuries of athletes participating in disciplines involving running (i.e. sprints, hurdles, jumps, combined events, middle distances, long distances, and marathon) were analysed.Results: Among the 12,233 registered athletes, 344 in-competition lower limb muscle injuries were reported (36% of all in-competition injuries). The proportion, incidence rates and injury burden of lower limb muscles injuries differed between disciplines for female and male athletes. The most frequently injured muscle group was hamstring in sprints, hurdles, jumps, combined events and male middle distances runners (43–75%), and posterior lower leg in female middle distances, male long distances, and female marathon runners (44–60%). Hamstring muscles injuries led to the highest burden in all disciplines, except for female middle distance and marathon and male long distance runners. Hamstring muscles injury burden was generally higher in disciplines requiring higher running velocities, and posterior lower leg muscle injuries higher in disciplines requiring lower running velocities.Conclusions: The present study shows discipline-specific injury location in competition context. Our findings suggest that the running velocity could be one of the factors that play a role in the occurrence/location of muscle injuries.
Published: 2021

10. Differential effects of adenylyl cyclase-protein kinase A cascade on shear-induced changes of sickle cell deformability

Author: Gokce Yavas, Philippe Joly, Giovanna Cannas, Yves Bertrand, Philippe Connes, Céline Renoux, Mine Turkay, Alexandra Gauthier, Buse Eglenen, Ozlem Yalcin, Arnaud Hot, Elif Ugurel, Ali Cenk Aksu, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Hospices Civils de Lyon (HCL), Service de Médecine Interne, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, medicine.medical_specialty, Erythrocytes, Physiology, Cell, Erythrocytes, Abnormal, Anemia, Sickle Cell, 030204 cardiovascular system & hematology, 030218 nuclear medicine & medical imaging, Pentoxifylline, Adenylyl cyclase, 03 medical and health sciences, chemistry.chemical_compound, Young Adult, [SCCO]Cognitive science, 0302 clinical medicine, stomatognathic system, Physiology (medical), Internal medicine, medicine, Erythrocyte deformability, Humans, Protein kinase A, ComputingMilieux_MISCELLANEOUS, technology, industry, and agriculture, Phosphodiesterase, Hematology, Cyclic AMP-Dependent Protein Kinases, Red blood cell, Endocrinology, medicine.anatomical_structure, chemistry, Female, Signal transduction, Cardiology and Cardiovascular Medicine, medicine.drug, Adenylyl Cyclases
Abstract: BACKGROUND Erythrocyte deformability is impaired in sickle cell disease (SCD). The regulation of cytoskeletal protein organization plays a key role in erythrocyte deformability. The activation of adenylyl cyclase (AC)/cAMP/Protein kinase A (PKA) signaling pathway was associated with increased deformability in healthy erythrocytes, however the role of this pathway in SCD is unknown. OBJECTIVE We evaluated mechanical responses of sickle red blood cells under physiological levels of shear stress and the possible link between their deformability and AC/cAMP/PKA signaling pathway. METHODS The shearing of sickle red blood cells at physiological level (5 Pa) and the measurement of deformability were performed by a laser assisted optical rotational cell analyzer (LORRCA). RESULTS Red blood cell deformability increased of 2.5-6.5% by blocking the activity of phosphodiesterase with Pentoxifylline (10μM) (p
Published: 2020

11. Impact of a 10 km running trial on eryptosis, red blood cell rheology, and electrophysiology in endurance trained athletes: a pilot study

Author: Mélanie Robert, Michèle Germain, Agnès Cibiel, Alexander Scheer, Elie Nader, Sarah Skinner, Philippe Connes, Céline Renoux, Emeric Stauffer, Stéphane Egée, David Monedero, Jules Hugonnet, Philippe Joly, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Blood Glucose, Male, medicine.medical_specialty, Erythrocytes, Physiology, Blood viscosity, Eryptosis, Hematocrit, Running, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Oxygen Consumption, Cell-Derived Microparticles, Heart Rate, Physiology (medical), Internal medicine, medicine, Humans, Orthopedics and Sports Medicine, Lactic Acid, Mean corpuscular volume, ComputingMilieux_MISCELLANEOUS, Oxygen saturation (medicine), medicine.diagnostic_test, Mean corpuscular hemoglobin concentration, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, Public Health, Environmental and Occupational Health, hemic and immune systems, 030229 sport sciences, General Medicine, Blood flow, Red blood cell, Endocrinology, medicine.anatomical_structure, Athletes, Hemorheology, Female, business, Perfusion, 030217 neurology & neurosurgery, circulatory and respiratory physiology, Physical Conditioning, Human
Abstract: Blood rheology is a key determinant of blood flow and tissue perfusion. There are still large discrepancies regarding the effects of an acute running exercise on blood rheological properties and red blood cell (RBC) physiology. We investigated the effect of a 10 km running trial on markers of blood rheology and RBC physiology in endurance trained athletes. Blood was sampled before and after the exercise to measure lactate and glucose, hematological and hemorheological parameters (blood viscosity, RBC deformability, and aggregation), eryptosis markers (phosphatidylserine and CD47 exposure, RBC reactive oxygen species), RBC-derived microparticles (RBC-MPs), and RBC electrophysiological activity. Weight was measured before and after exercise. Peripheral oxygen saturation and heart rate were monitored before and during the trial. Blood lactate and glucose levels increased after exercise and subjects significantly lost weight. All athletes experienced a significant fall in oxygen saturation. Mean corpuscular volume (MCV) was increased from 95.1 ± 3.2 to 96.0 ± 3.3 and mean corpuscular hemoglobin concentration (MCHC) decreased after exercise suggesting a slight RBC rehydration. Exercise increased RBC deformability from 0.344 ± 0.04 to 0.378 ± 0.07, decreased RBC aggregates strength and blood viscosity, while hematocrit (Hct) remained unaffected. While RBC electrophysiological recording suggested a modulation in RBC calcium content and/or chloride conductance, eryptosis markers and RBC-MPs were not modified by the exercise. A 10 km acute running exercise had no effect on RBC senescence and membrane blebbing. In contrast, this exercise increased RBC deformability, probably through rehydration process which resulted in a decrease in blood viscosity.
Published: 2020

12. The therapeutic role of motor imagery during the chronic phase after total knee arthroplasty: a pilot randomized controlled trial

Author: Nady Hoyek, Franck Di Rienzo, Marcel Moukarzel, Aymeric Guillot, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), and Université Saint-Esprit de Kaslik (USEK)
Subjects: Male, 030506 rehabilitation, medicine.medical_specialty, Imagery, Psychotherapy, medicine.medical_treatment, Population, Physical Therapy, Sports Therapy and Rehabilitation, Pilot Projects, Walk Test, Timed Up and Go test, law.invention, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Physical medicine and rehabilitation, Motor imagery, Randomized controlled trial, law, medicine, Humans, Muscle Strength, Range of Motion, Articular, education, Arthroplasty, Replacement, Knee, Physical Therapy Modalities, ComputingMilieux_MISCELLANEOUS, Aged, education.field_of_study, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, Rehabilitation, Recovery of Function, Middle Aged, Arthroplasty, Female, 0305 other medical science, Range of motion, business, Motor learning, human activities, 030217 neurology & neurosurgery, Oxford knee score
Abstract: BACKGROUND There is now ample evidence that motor imagery contributes to enhance motor learning and promote motor recovery in patients with motor disorders. Whether motor imagery practice is likely to facilitate mobility in patients suffering from knee osteoarthritis, at 6-months after total knee arthroplasty, remains unknown. AIM This trial was designed to evaluate the therapeutic effectiveness of implementing motor imagery into the classical course of physical therapy at 6-months after total knee arthroplasty. DESIGN Randomized controlled trial. POPULATION Twenty-four patients with unilateral total knee arthroplasty were assigned to a motor imagery or control group in a test-retest procedure, following a rehabilitation program as outpatients. METHODS During both the pre- and post-test, a set of strength and functional mobility measures were assessed: quadriceps strength, peak knee flexion during the swing phase, performance at the timed up and go test, stair climbing test, and 6-minute walk test, and finally Oxford knee score. In addition to a common physical therapy program, the motor imagery group practiced additional motor imagery exercises, while participants of the control group were subjected to a period of neutral activities for an equivalent amount of time. RESULTS Data provided evidence that motor imagery enhanced the quadriceps muscle strength of the operated knee (F (1, 22)=10.36, P=0.003), improved the peak knee flexion during the swing phase (F (1, 22)=31.52, P
Published: 2019

13. How Fast Is a Horizontal Squat Jump?

Author: Jérémy Rossi, Jean-Benoit Morin, Jean Romain Rivière, Pierre Samozino, Pedro Jiménez-Reyes, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire Motricité Humaine Expertise Sport Santé (LAMHESS), Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université de Toulon (UTLN)-Université Côte d'Azur (UCA)
Subjects: Adult, Male, Strength training, Movement, [SDV]Life Sciences [q-bio], Physical Therapy, Sports Therapy and Rehabilitation, Squat, Plyometric Exercise, Body weight, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Squat jump, Task Performance and Analysis, Humans, Orthopedics and Sports Medicine, Muscle Strength, Power output, ComputingMilieux_MISCELLANEOUS, Mathematics, Body Weight, Resistance Training, 030229 sport sciences, Mechanics, Biomechanical Phenomena, Lower Extremity, 030217 neurology & neurosurgery
Abstract: Velocity strength training requires exercise modalities that allow athletes to reach very high movement velocity, which is limited during vertical movements involving body weight. Purpose: To quantify the mechanical outputs developed during horizontal squat jumps (HSJs), notably the movement velocity, in comparison with vertical squat jumps (SJs) with and without loads. Methods: Thirteen healthy male athletes performed SJs without additional loads (SJ0) and with a load of ∼60% of body mass (SJ60), and during HSJs performed lying on a roller device with (assisted HSJ [AHSJ]) and without (HSJ) rubber-band assistance. Instantaneous lower-limb extension velocity, force, and power output were measured and averaged over the push-off phase. Results: The force was significantly higher during SJ60 than during SJ0, which was higher than during HSJ and AHSJ. Extension velocity was significantly different across all conditions, with 0.86 (0.07), 1.29 (0.10), 1.59 (0.19), and 1.83 (0.19) m·s−1 for SJ60, SJ0, HSJ, and AHSJ conditions, respectively. Differences in force and velocity values between SJ0 and the other conditions were large to extremely large. Differences were observed in power values only between SJ60 and SJ0, SJ60 and AHSJ, and SJ0 and HSJ. Conclusions: HSJ modalities allow athletes to reach very to extremely largely greater lower-limb extension velocities (HSJ +24.0% [16%], AHSJ +42.8% [17.4%]) compared to those achieved during SJ0. HSJ and AHSJ modalities are inexpensive and practical modalities to train limb-extension velocity capabilities, that is, the ability of the neuromuscular system to produce force at high contraction velocities.
Published: 2018

14. Muscle Oxygen Supply and Use in Type 1 Diabetes, From Ambient Air to the Mitochondrial Respiratory Chain: Is There a Limiting Step?

Author: Aurélien Descatoire, Pierre Fontaine, Régis Matran, Frédéric N. Daussin, Serge Berthoin, Erwan Leclair, Phanélie Berthon, Julien Aucouturier, Elsa Heyman, Adrien Combes, Valerie Wieczorek, Robert Caiazzo, Sémah Tagougui, Gaelle Marais, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université de Lille-Université du Littoral Côte d'Opale (ULCO), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Recherche translationnelle sur le diabète - U 1190 (RTD), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Service de chirurgie générale et endocrinienne, Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (GI3M), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille, LillOA, Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Physiotherapy, Human Physiology and Anatomy, Human Physiology and Sports Physiotherapy Research Group, Université de Lille, Univ. Artois, Univ. Littoral Côte d’Opale, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369, Recherche translationnelle sur le diabète (RTD) - U1190, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 [URePSSS], and Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Subjects: Male, Oxyhemoglobins/analysis, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Blood volume, Oxygen Consumption/physiology, 0302 clinical medicine, Exercise/physiology, Diabetes Mellitus, Type 1/metabolism, 030212 general & internal medicine, Spectroscopy, Near-Infrared, Oxygen–haemoglobin dissociation curve, 3. Good health, [SDV] Life Sciences [q-bio], Mitochondrial respiratory chain, medicine.anatomical_structure, RESPIRATION, young adult, Female, Adult, medicine.medical_specialty, Adolescent, Vastus lateralis muscle, Cell Respiration, 030209 endocrinology & metabolism, Case-control studies, Electron Transport, 03 medical and health sciences, Oxygen Consumption, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Aerobic exercise, Humans, Muscle, Skeletal, Exercise, Advanced and Specialized Nursing, Muscle, Skeletal/metabolism, business.industry, Skeletal muscle, Mitochondria, Muscle/metabolism, medicine.disease, Mitochondria, Muscle, Oxygen/analysis, Oxygen, Endocrinology, Diabetes Mellitus, Type 1, Oxyhemoglobins, Hemoglobin, business, Electron Transport/physiology
Abstract: OBJECTIVE Long before clinical complications of type 1 diabetes (T1D) develop, oxygen supply and use can be altered during activities of daily life. We examined in patients with uncomplicated T1D all steps of the oxygen pathway, from the lungs to the mitochondria, using an integrative ex vivo (muscle biopsies) and in vivo (during exercise) approach. RESEARCH DESIGN AND METHODS We compared 16 adults with T1D with 16 strictly matched healthy control subjects. We assessed lung diffusion capacity for carbon monoxide and nitric oxide, exercise-induced changes in arterial O2 content (SaO2, PaO2, hemoglobin), muscle blood volume, and O2 extraction (via near-infrared spectroscopy). We analyzed blood samples for metabolic and hormonal vasoactive moieties and factors that are able to shift the O2-hemoglobin dissociation curve. Mitochondrial oxidative capacities were assessed in permeabilized vastus lateralis muscle fibers. RESULTS Lung diffusion capacity and arterial O2 transport were normal in patients with T1D. However, those patients displayed blunted exercise-induced increases in muscle blood volume, despite higher serum insulin, and in O2 extraction, despite higher erythrocyte 2,3-diphosphoglycerate. Although complex I– and complex II–supported mitochondrial respirations were unaltered, complex IV capacity (relative to complex I capacity) was impaired in patients with T1D, and this was even more apparent in those with long-standing diabetes and high HbA1c. O2max was lower in patients with T1D than in the control subjects. CONCLUSIONS Early defects in microvascular delivery of blood to skeletal muscle and in complex IV capacity in the mitochondrial respiratory chain may negatively impact aerobic fitness. These findings are clinically relevant considering the main role of skeletal muscle oxidation in whole-body glucose disposal.
Published: 2019

15. Effects of Action Observation and Action Observation Combined with Motor Imagery on Maximal Isometric Strength

Author: Ursula Debarnot, Christian Collet, Tadhg E. MacIntyre, Franck Di Rienzo, Pierric Joassy, Yoann Blache, Thiago Ferreira Dias Kanthack, Aymeric Guillot, Christophe Hautier, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: 0301 basic medicine, Adult, Male, medicine.medical_specialty, Elbow flexor, Isometric exercise, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Motor Activity, Biceps, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Motor imagery, [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication, Motor cognition, Isometric Contraction, Motor system, Elbow Joint, medicine, Humans, Force platform, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Electromyography, General Neuroscience, 030104 developmental biology, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, Action observation, Arm, Imagination, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Female, Psychology, 030217 neurology & neurosurgery
Abstract: Action observation (AO) alone or combined with motor imagery (AO + MI) has been shown to engage the motor system. While recent findings support the potential relevance of both techniques to enhance muscle function, this issue has received limited scientific scrutiny. In the present study, we implemented a counterbalanced conditions design where 21 participants performed 10 maximal isometric contractions (12-s duration) of elbow flexor muscles against a force platform. During the inter-trial rest periods, participants completed i) AO of the same task performed by an expert athlete, ii) AO + MI, i.e. observation of an expert athlete while concurrently imagining oneself performing the same task, and iii) watching passively a video documentary about basketball shooting (Control). During force trials, we recorded the total force and integrated electromyograms from the biceps brachii and anterior deltoideus. We also measured skin conductance from two finger electrodes as an index of sympathetic nervous system activity. Both AO and AO + MI outperformed the Control condition in terms of total force (2.79–3.68%, p
Published: 2019

16. Short Trail Running Race

Author: Jean-Benoit Morin, Fabrice Vercruyssen, Marcus Peikriszwili Tartaruga, Christopher S. Easthope, Jeanick Brisswalter, Sabine Ehrström, Laboratory of Human Motricity, Education Sport and Health, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université de Toulon (UTLN), University of Zurich, and Ehrström, Sabine
Subjects: Adult, Male, medicine.medical_specialty, Physical Therapy, [SDV]Life Sciences [q-bio], 610 Medicine & health, Physical Therapy, Sports Therapy and Rehabilitation, Sports Therapy and Rehabilitation, Athletic Performance, Running, 03 medical and health sciences, Oxygen Consumption, 2732 Orthopedics and Sports Medicine, 0302 clinical medicine, Physical medicine and rehabilitation, Humans, Medicine, Orthopedics and Sports Medicine, Muscle Strength, 3612 Physical Therapy, Sports Therapy and Rehabilitation, Fatigue, business.industry, VO2 max, 030229 sport sciences, Middle Aged, Exercise Test, Physical Endurance, Running economy, Muscle strength, 10046 Balgrist University Hospital, Swiss Spinal Cord Injury Center, Ventilatory threshold, business, 030217 neurology & neurosurgery
Abstract: International audience; PURPOSE:This study aimed to examine the extent to which the classical physiological variables of endurance running performance (maximal oxygen uptake (V˙O2max), %V˙O2max at ventilatory threshold (VT), and running economy (RE)) but also muscle strength factors contribute to short trail running (TR) performance.METHODS:A homogeneous group of nine highly trained trail runners performed an official TR race (27 km) and laboratory-based sessions to determine V˙O2max, %V˙O2max at VT, level RE (RE0%) and RE on a +10% slope, maximal voluntary concentric and eccentric knee extension torques, local endurance assessed by a fatigue index (FI), and a time to exhaustion at 87.5% of the velocity associated with V˙O2max. A simple regression method and commonality analysis identifying unique and common coefficients of each independent variable were used to determine the best predictors for the TR race time (dependent variable).RESULTS:Pearson correlations showed that FI and V˙O2max had the highest correlations (r = 0.91 and r = -0.76, respectively) with TR performance. The other selected variables were not significantly correlated with TR performance. The analysis of unique and common coefficients of relative V˙O2max, %V˙O2max at VT, and RE0% provides a low prediction of TR performance (R = 0.48). However, adding FI and RE on a +10% slope (instead of RE0%) markedly improved the predictive power of the model (R = 0.98). FI and V˙O2max showed the highest unique (49.8% and 20.4% of total effect, respectively) and common (26.9% of total effect) contributions to the regression equation.CONCLUSIONS:The classic endurance running model does not allow for meaningful prediction of short TR performance. Incorporating more specific factors into TR such as local endurance and gradient-specific RE testing procedures should be considered to better characterize short TR performance.
Published: 2018

17. The therapeutic role of motor imagery during the acute phase after total knee arthroplasty: a pilot study

Author: Moukarzel, Marcel, Di Rienzo, Franck, Lahoud, Jean-Claude, Hoyek, Fadi, Collet, Christian, Guillot, Aymeric, Hoyek, Nady, Rienzo, Di, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), and Université Saint-Esprit de Kaslik (USEK)
Subjects: Male, 030506 rehabilitation, medicine.medical_specialty, Imagery, Psychotherapy, Knee Joint, [SDV]Life Sciences [q-bio], [SHS.EDU]Humanities and Social Sciences/Education, medicine.medical_treatment, Total knee arthroplasty, Pilot Projects, Postoperative recovery, [SCCO]Cognitive science, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Motor imagery, Humans, Medicine, In patient, Range of Motion, Articular, Arthroplasty, Replacement, Knee, Physical Therapy Modalities, ComputingMilieux_MISCELLANEOUS, Aged, Pain Measurement, Pain, Postoperative, Rehabilitation, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, Recovery of Function, Middle Aged, Treatment Outcome, Female, 0305 other medical science, business, 030217 neurology & neurosurgery
Abstract: The aim of this study was to measure physical and functional outcomes during the acute postoperative recovery in patients who underwent total knee arthroplasty. Motor imagery has been shown to decrease pain and promote functional recovery after both neurological and peripheral injuries. Yet, whether motor imagery can be included as an adjunct effective method into physical therapy programs following total knee arthroplasty remains a working hypothesis that we aim to test in a pilot study.Twenty volunteers were randomly assigned to either a motor imagery or a control group. Pain, range of motion, knee girth as well as quadriceps strength and Timed Up and Go Test time were the dependent variables during pre-test and post-test.The motor imagery group exhibited larger decrease of ipsilateral pain and knee girth, a slightly different evolution of range of motion and an increase of ipsilateral quadriceps strength compared to the control group. No effects of motor imagery on Timed Up and Go Test scores were observed.Implementing motor imagery practice into the course of physical therapy enhanced various physical outcomes during acute postoperative recovery after total knee arthroplasty. According to this pilot study, motor imagery might be relevant to promote motor relearning and recovery after total knee arthroplasty.Partial effect-sizes should be conducted in the future. Implications for rehabilitation Adding motor imagery to physical therapy sessions during the acute period following total knee arthroplasty: • Enhances quadriceps strength. • Alleviates pain. • Enhances range of motion. • Does not have any effect on basic functional mobility. • Does not have any effect on knee girth.
Published: 2017

18. Effects of hydroxyurea on blood rheology in sickle cell anemia: A two-years follow-up study

Author: Yann Lamarre, Xavier Waltz, Marc Romana, Marie-Dominique Hardy-Dessources, Philippe Connes, Berenike Möckesch, Sophie Antoine-Jonville, Keyne Charlot, Nathalie Lemonne, Yohann Garnier, Maryse Etienne-Julan, Unité Transversale de la Drépanocytose, CHU Pointe-à-Pitre/Abymes [Guadeloupe], Université des Antilles (Pôle Guadeloupe), Université des Antilles (UA), Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Transfusion Sanguine [Paris] (INTS), Dynamique des Structures et Interactions des Macromolécules Biologiques - Pôle de La Réunion (DSIMB Réunion), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Transfusion Sanguine [Paris] (INTS), Adaptation, Climat Tropical, Exercice et Santé (ACTES), Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Hôpital Ricou, Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA), Laboratory of Excellence GR-Ex 'The red cell: from genesis to death', PRES Sorbonne Paris Cité, Université des Antilles et de la Guyane (UAG), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, medicine.medical_specialty, Physiology, [SDV]Life Sciences [q-bio], Blood viscosity, Anemia, Sickle Cell, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Antisickling Agents, Physiology (medical), White blood cell, Internal medicine, Fetal hemoglobin, medicine, Humans, Hydroxyurea, Platelet, blood rheology, business.industry, Follow up studies, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, Hematology, medicine.disease, Acute chest syndrome, Sickle cell anemia, 3. Good health, medicine.anatomical_structure, clinical severity, 030220 oncology & carcinogenesis, sickle cell disease, Female, Hemoglobin, Rheology, Cardiology and Cardiovascular Medicine, business, Follow-Up Studies, 030215 immunology
Abstract: International audience; The aim of the present study was to test the effects of hydroxyurea (HU) therapy on clinical, hematological and hemorheological parameters in adult patients with sickle cell anemia (SCA). Hematological and hemorheological parameters were measured in 28 SCA patients before HU therapy (i.e., baseline) and at 6, 12 and 24 months of treatment. RBC deformability was determined by ektacytometry at 30 Pa. RBC aggregation properties were investigated by light-backscatter method. Blood viscosity was measured at 225 s-1 by a cone-plate viscometer. The rates of vaso-occlusive crises and acute chest syndrome were lower at 1 and 2 years of HU therapy compared to baseline. The proportion of patients with leg ulcers tended to decrease after 2 years of treatment. Hemoglobin oxygen saturation improved with HU therapy. HU therapy induced a decrease of platelet and white blood cell counts and a rise in fetal hemoglobin level and mean cell volume. While hemoglobin concentrations increased under HU, blood viscosity remained unchanged all along the study. RBC deformability increased over baseline values at 6 months of HU therapy and continued to rise until the end of the follow-up period. In conclusion, the improvement in RBC deformability probably compensates the increase of hemoglobin on blood viscosity and participates to the improvement of the clinical status of patients.
Published: 2017

19. Superimposition of elastic and nonelastic compression bandages

Author: Jérôme Molimard, Clothilde Helouin-Desenne, Pierre Badel, Fanette Chassagne, Pascal Giraux, Reynald Convert, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, Supine position, Compression Bandage, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Posture, 030204 cardiovascular system & hematology, Sitting, Elastic bandage, Young Adult, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Compression Bandages, Interface pressure, Pressure, medicine, Humans, Superimposition, ComputingMilieux_MISCELLANEOUS, Spiral, Aged, Venous Thrombosis, business.industry, Middle Aged, Female, Surgery, Cardiology and Cardiovascular Medicine, business, Bandage, Biomedical engineering
Abstract: The objective of this study was to investigate the pressure applied by superimposed bandages and to compare it with the pressure applied by single-component bandages.Six different bandages, composed of one elastic bandage, one nonelastic bandage, or both, were applied in a spiral pattern on both legs of 25 patients at risk of venous thrombosis as a consequence of central or peripheral motor deficiency. Pressure was measured at four measurement points on the leg (B1 and C on the medial and lateral sides of the leg) and in three positions: supine, sitting, and standing.The two single bandages applied similar pressure in the supine position. Their superimposition showed different pressure levels (P .05) but similar static stiffness index, depending on the order in which the bandage components were applied on the leg. The highest interface pressure was measured at point B1 on the medial side of the leg. This point also showed the highest pressure increase from supine to standing position. The pressure applied by the superimposition of two bandages was computed as a linear combination of the pressure applied by each single component (with a constant term set to 0). However, this linear combination did not properly fit the experimental pressure measurements.The order of bandage application showed a significant impact on interface pressure. However, the poor correlation between the pressure applied by each bandage component and the pressure resulting from their superimposition underlined the poor understanding of interface pressure generated by the superimposition of compression bandages and should lead to further investigations.
Published: 2017

20. Understanding wear in dual mobility total hip replacement: first generation explant wear patterns

Author: Jean Geringer, Rémi Philippot, Thomas Neri, Bertrand Boyer, Alexandre Di Iorio, Frédéric Farizon, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), and Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F - 42023 Saint-Etienne France
Subjects: Male, musculoskeletal diseases, medicine.medical_specialty, Arthroplasty, Replacement, Hip, [SDV]Life Sciences [q-bio], Joint Dislocations, Total hip replacement, Dentistry, Prosthesis Design, [SPI]Engineering Sciences [physics], 03 medical and health sciences, Imaging, Three-Dimensional, 0302 clinical medicine, medicine, Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, Qualitative Research, 030222 orthopedics, business.industry, Dual mobility, First generation, Prosthesis Failure, Surgery, Orthopedic surgery, Hip Prosthesis, Implant, business, human activities, Total hip arthroplasty, Explant culture
Abstract: Dual mobility was introduced by Pr. G. Bousquet to improve the dislocation risk of total hip arthroplasty (THR). The wear mechanisms of this implant remain to be understood. Could enhanced explant wear analysis explain liner wear behaviour?Sixty-six explanted liners were selected. Non-destructive 3D scanning and heat colour treatment allowed reporting wear patterns on both convex and concave sides of the liners.For many liners, liner convexity wear was found to be homogeneous, and linked to a longer implantation time. A few liners had signs of blocking of the convexity, leading to excessive internal damage. Intra prosthetic dislocations were found to only have internal circular wear without liner penetration.Heat colour treatment showed its effectiveness for highlighting wear patterns. Optimal convexity mobility seemed to be leading to a longer implantation time, with decreased contact stresses on concavity. The retentive rim should have a distinct wear analysis.
Published: 2016

21. Evolution of blood rheology and its relationship to pulmonary hemodynamic during the first days of exposure to moderate altitude

Author: Jorge Lopez Ayerbe, Patrick Mucci, Philippe Connes, Fabienne Durand, Antoine Raberin, Elie Nader, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université de Lille-Université du Littoral Côte d'Opale (ULCO), Laboratoire Européen Performance Santé Altitude (LEPSA), Université de Perpignan Via Domitia (UPVD), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), and Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - EA 7369 (URePSSS)
Subjects: Adult, Male, medicine.medical_specialty, Pulmonary Circulation, Physiology, Blood viscosity, Hemodynamics, 030204 cardiovascular system & hematology, Hematocrit, Acclimatization, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, [SCCO]Cognitive science, Young Adult, 0302 clinical medicine, Physiology (medical), Internal medicine, Hypoxic pulmonary vasoconstriction, medicine, Humans, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, business.industry, Altitude, Hematology, Hemoconcentration, Healthy Volunteers, Red blood cell, medicine.anatomical_structure, Vascular resistance, Cardiology, Cardiology and Cardiovascular Medicine, business, Rheology
Abstract: Blood rheology and hemodynamic parameters have never been explored together during acclimatization to altitude. This study aimed to investigate changes in blood rheology parameters and pulmonary hemodynamics during the first days of real moderate altitude exposure.Seventeen athletes were tested at sea-level, 20 hours after their arrival at 2,400 meters of altitude (H1) and five days later (H2). Blood was sampled to analyze red blood cell (RBC) aggregation, blood viscosity and hematocrit. Pulmonary arterial pressure (PAP), pulmonary capillary pressure (Pcap) and pulmonary vascular resistance (PVR) were assessed by echocardiography.We observed a rise in hematocrit, blood viscosity, RBC aggregation, PAP, Pcap and PVR between sea-level and H1. In H2, RBC aggregation, hematocrit, PAP, Pcap and PVR remained different compared to sea-level and no difference was observed between H1 and H2. Blood viscosity decreased in H2 and returned to sea-level values.Our results suggest that hemoconcentration occurring within the first hours of altitude exposure increased blood viscosity, which contributed to the changes in pulmonary hemodynamic. When blood viscosity decreased in H2, no change occurred in pulmonary hemodynamic parameters suggesting that hypoxic pulmonary vasoconstriction was still present. The elevated RBC aggregation observed after in H2 could participate in the increase of Pcap.
Published: 2019

22. Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients

Author: Thomas Dietz, Aram Prokop, Bianca Collins, Wilhelm Bloch, Max Jerke, Philippe Connes, Céline Renoux, Alexander Schenk, Daniel A. Bizjak, Philippe Joly, Elie Nader, Marijke Grau, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Male, 0301 basic medicine, Erythrocytes, Lipid peroxidation, [SCCO]Cognitive science, chemistry.chemical_compound, 0302 clinical medicine, Erythrocyte deformability, ComputingMilieux_MISCELLANEOUS, reproductive and urinary physiology, Multidisciplinary, biology, hemic and immune systems, Nitric oxide synthase, medicine.anatomical_structure, Medicine, Female, Signal Transduction, circulatory and respiratory physiology, medicine.medical_specialty, Adolescent, Anemia, Science, Anemia, Sickle Cell, Article, Nitric oxide, 03 medical and health sciences, Erythrocyte Deformability, Internal medicine, medicine, Humans, Exercise physiology, Exercise, business.industry, Sickle cell disease, Diagnostic markers, medicine.disease, Oxidative Stress, Red blood cell, 030104 developmental biology, Endocrinology, chemistry, Case-Control Studies, biology.protein, Nitric Oxide Synthase, Ventilatory threshold, business, 030217 neurology & neurosurgery
Abstract: Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subjects reached the first ventilatory threshold (VT 1). Blood was sampled at rest and at VT 1. At rest, free haemoglobin level was higher and RBC count, haemoglobin and haematocrit were lower in SCA compared to AA. RBC deformability was lower in SCA. Exercise had no effect on the tested parameters. RBC NO level was higher in SCA compared to AA at rest and significantly decreased after exercise in SCA. This might be related to a reduction in RBC-NO synthase (RBC-NOS) activation which was only observed in SCA after exercise. Free radical levels were higher in SCA at rest but concentration was not affected by exercise. Marker for lipid peroxidation and antioxidative capacity were similar in SCA and AA and not affected by exercise. In conclusion, a single acute submaximal bout of exercise has no deleterious effects on RBC deformability or oxidative stress markers in SCA, and seems to modulate RBC-NOS signalling pathway.
Published: 2019

23. Impact of Hip Flexion Angle on Unilateral and Bilateral Nordic Hamstring Exercise Torque and High-Density Electromyography Activity

Author: Jean-Patrick Giacomo, Neil J. Cronin, Pauline Gerus, Jean-Benoit Morin, Johan Lahti, András Hegyi, Laboratoire Motricité Humaine Expertise Sport Santé (LAMHESS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université de Toulon (UTLN)-Université Côte d'Azur (UCA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Adult, Male, medicine.medical_specialty, Knee Joint, medicine.medical_treatment, High density, Hamstring Muscles, Physical Therapy, Sports Therapy and Rehabilitation, Electromyography, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, medicine, Humans, Range of Motion, Articular, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Exercise, ComputingMilieux_MISCELLANEOUS, Hamstring injury, Rehabilitation, medicine.diagnostic_test, business.industry, 030229 sport sciences, General Medicine, medicine.disease, Torque, Athletes, population characteristics, Hip Joint, business, 030217 neurology & neurosurgery, Hamstring, Hip flexion, Muscle Contraction
Abstract: In the bilateral Nordic hamstring exercise (NHE), hamstrings operate at relatively short lengths, which may limit the efficacy of the NHE in hamstring injury prevention.To examine knee flexion torque and biceps femoris long head (BFLH) and semitendinosus (ST) high-density electromyography (EMG) activity during the unilateral and bilateral NHE, performed with either neutral (NHE0) or 90°-flexed (NHE90) hips.In this laboratory study, exercises were performed on a novel device at the eccentric 1-repetition maximum load defined for 90° to 15° of knee range of motion. Torque and EMG signals normalized to maximal voluntary isometric activity were compared in different phases of the exercises with statistical parametric mapping.The EMG levels were lower in NHE90 than in NHE0, mainly in the second half of the movement. Knee flexor eccentric torque was higher in NHE90 than in NHE0 from the beginning to 87% of the bilateral movement, and over the entire unilateral movement. In NHE0, ST activity compared to BFLH activity was higher during the initial movement phase and lower when the movement was close to knee extension. Torque and EMG activity were generally similar in the bilateral and unilateral modes.If performed with neutral hips, the NHE selectively activates the BFLH near full knee extension. Performing the NHE with hips flexed to 90° is preferable when higher passive torque and ST selectivity are targeted at a longer muscle length. Performing these exercises unilaterally could help train each limb separately, with similar torque and EMG output to those of the bilateral conditions.
Published: 2019

24. Magnesium transport and homeostasis-related gene expression in skeletal muscle of young and old adults: analysis of the transcriptomic data from the PROOF cohort Study

Author: Coudy-Gandilhon, Cécile, Gueugneau, Marine, Taillandier, Daniel, Combaret, Lydie, Polge, Cécile, Roche, Frédéric, Barthélémy, Jean-Claude, Feasson, Léonard, Maier, Jeanette, Mazur, André, Béchet, Daniel, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Unité de nutrition et métabolisme protéique, Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Université Clermont Auvergne (UCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Jean Monnet [Saint-Étienne] (UJM), Unité de Myologie, Centre Référent Maladies Neuromusculaires Rares Rhône-Alpes, Unité de nutrition cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Cancer Resistance Exploring and Targeting (CREaT), Université d'Auvergne - Clermont-Ferrand I (UdA), Service de Physiologie Clinique et de l'Exercice [CHU de Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Università degli Studi di Milano = University of Milan (UNIMI), French government IDEX-ISITE initiative 16-IDEX-0001 (CAP 20-25), Hôpital Nord - Faculté de MédecineJacques Lisfranc, Service de Physiologie Clinique et de l'Exercice, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Service de Physiologie Clinique et de l’Exercice, Faculté de Médecine Jacques Lisfranc, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), and Department of Biomedical and Clinical Sciences, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, University of Milan, Milan, Italy
Subjects: Adult, Male, [SDV]Life Sciences [q-bio], Skeletal muscle, magnesium, Cohort Studies, sarcopenia, Young Adult, MESH: Magnesium, Homeostasis, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Muscle, Skeletal, MESH: Cohort Studies, Aged, Oligonucleotide Array Sequence Analysis, MESH: Aged, MESH: Muscle, Skeletal, MESH: Humans, MESH: Transcriptome, aging, MESH: Adult, MESH: Male, MESH: Young Adult, MESH: Homeostasis, MESH: Oligonucleotide Array Sequence Analysis, transcriptome, magnesium transporters
Abstract: International audience; Magnesium (Mg2+) is critical for a number of biological processes and 25% body Mg2+ is located in the skeletal muscle. Mg2+ transport and homeostasis systems (MgTHs) regulate intracellular Mg2+ concentration and muscle MgTHs are thus related to whole body Mg2+ homeostasis. Nonetheless, few studies have investigated the regulation of muscle MgTHs under (patho)physiological conditions. Herein, we assessed the relationship between the expression of MgTHs genes (Trpm6, Trpm7, Magt1, Mrs2, Cnnm1-4, Slc41a1-3) and relevant pathways in human sarcopenia, which is one of the most dramatic physiologic changes affecting the human body. Transcriptomic data were compared between young adult (YO, 22 y, n = 11) and old (EL, 73 y, n = 13) men from the PROOF cohort. MgTH mRNA levels did not change with aging, with the exception of a slight decrease for Slc41a3. Nevertheless, interindividual variations of mRNA levels revealed strong correlations between MgTHs in the YO group, while few were maintained in the EL muscle. Moreover, in the YO muscle, different clusters of MgTH mRNAs strongly correlated with divers physiological (BMI, blood pressure) and muscle characteristics (intramyocellular droplets, capillarization); however, most correlations changed or disappeared in the EL muscle. Further investigations of the whole transcriptome identified several sets of mRNAs correlated with defined MgTHs. There again was a sharp difference between YO and EL muscles, as the number of mRNAs correlated with MgTHs strongly decreased with aging. Gene ontology analyses of these sets of correlated mRNAs revealed 6 biological processes common to YO and EL, 3 specific to the YO (RNA processing, translation, respiration), and 2 (regulation of catabolic process, Wnt signaling) to the EL muscle. Overall, these observations lead to questions about potential resilience to muscle Mg2+ homeostasis in the elderly.
Published: 2019

25. Combined and differential effects of alpha‐thalassemia and HbF‐quantitative trait loci in Senegalese hydroxyurea‐free children with sickle cell anemia

Author: Ibrahima Diagne, Rokhaya Ndiaye Diallo, Pape Amadou Diop, Papa Madieye Gueye, Fatou Gueye Tall, Aynina Cisse, Cyril Martin, Philippe Connes, Céline Renoux, Philomène Lopez Sall, Indou Deme Ly, Philippe Joly, El Hadji Malick Ndour, Faculté de Médecine, de Pharmacie et d’Odontostomatologie [Dakar, Sénégal] (FMPOS), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre Hospitalier National d'Enfants Albert Royer, Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, Genotype, Composite score, Quantitative Trait Loci, Anemia, Sickle Cell, Alpha-thalassemia, Quantitative trait locus, Gastroenterology, [SCCO]Cognitive science, 03 medical and health sciences, 0302 clinical medicine, sickle cell anemia, alpha-Thalassemia, G6PD deficiency, hemic and lymphatic diseases, Internal medicine, Humans, Medicine, Clinical severity, HbF QTL, Young adult, Child, Fetal Hemoglobin, Hemoglobin H, business.industry, Hematology, Senegal haplotype, genetic modifiers, medicine.disease, Differential effects, Senegal, Sickle cell anemia, 3. Good health, Oncology, 030220 oncology & carcinogenesis, Pediatrics, Perinatology and Child Health, Female, business, 030215 immunology
Abstract: International audience; BACKGROUND: Our objective was to investigate the combined and differential effects of alpha-thalassemia -3.7 kb deletion and HbF-promoting quantitative trait loci (HbF-QTL) in Senegalese hydroxyurea (HU)-free children and young adults with sickle cell anemia (SCA).PROCEDURE: Steady-state biological parameters and vaso-occlusive crises (VOC) requiring emergency admission were recorded over a 2-year period in 301 children with SCA. The age of the first hospitalized VOC was also recorded. These data were correlated with the alpha-globin and HbF-QTL genotypes. For the latter, three different genetic loci were studied (XmnI, rs7482144; BCL11A, rs1427407; and the HBS1L-MYB region, rs28384513) and a composite score was calculated, ranging from zero (none of these three polymorphisms) to six (all three polymorphisms at the homozygous state).RESULTS: A positive clinical impact of the HbF-QTL score on VOC rate, HbF, leucocytes, and C-reactive protein levels was observed only for patients without alpha-thalassemia deletion. Conversely, combination of homozygous -3.7 kb deletion with three to six HbF-QTL was associated with a higher VOC rate. The age of the first hospitalized VOC was delayed for patients with one or two alpha-thalassemia deletions and at least two HbF-QTL.CONCLUSION: Alpha-thalassemia -3.7 kb deletion and HbF-QTL are modulating factors of SCA clinical severity that interact with each other. They should be studied and interpreted together and not separately, at least in HU-free children.
Published: 2019

26. Anatomic risk factor for meniscal lesion in association with ACL rupture

Author: Sébastien Lustig, Elvire Servien, Robert A. Magnussen, Philippe Neyret, Romain Gaillard, Cécile Batailler, Service de Chirurgie Orthopédique [Centre Albert Trillat], Centre Albert Trillat [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL)-Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Ohio State University [Columbus] (OSU), Laboratoire de Biomécanique et Mécanique des Chocs (LBMC UMR T9406 ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Gustave Eiffel, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: CT scan, Adult, Male, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, Adolescent, Menisci, Tibial, ANATOMIE, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Sex Factors, lcsh:Orthopedic surgery, Risk Factors, medicine, Humans, Orthopedics and Sports Medicine, Femur, Single-Blind Method, Tibia, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Risk factor, Meniscal tear, Retrospective Studies, 030203 arthritis & rheumatology, 030222 orthopedics, Anterior Cruciate Ligament Reconstruction, business.industry, ACL, Anterior Cruciate Ligament Injuries, Middle Aged, musculoskeletal system, Sagittal plane, Surgery, lcsh:RD701-811, Acl rupture, medicine.anatomical_structure, Concomitant, Orthopedic surgery, Female, lcsh:RC925-935, Anatomy, business, Meniscal lesions, Research Article
Abstract: Background To assess anatomic risk factors for meniscal lesions in association with acute ACL rupture. The primary hypothesis was that tibiofemoral anatomic measures will be different in those with and without concomitant meniscus tears. Methods A retrospective review of patients who underwent acute ACL reconstruction in the department was performed. All patients underwent a postoperative CT scan. The concavity and/or convexity on the femur and the tibia were measured by two blinded observers on the sagittal plane with different ratios, and these measures were compared in patients with and without meniscus tears in each compartment. Intra- and inter-rater reliabilities were assessed. Results Four hundred twelve patients (268 males and 144 females) were included from October 2012 to February 2015. One hundred sixty-seven patients had a medial meniscal tear (119 males/48 females), and 100 had a lateral meniscal tear (80 males/20 females). The mean time from injury to surgery was 3 months. The average ICC for all measurements was 0.87 (range 0.82–0.98) indicating good reliability. The medial femoral condyle was noted to be significantly longer than the medial tibial plateau in the sagittal plane in patients with a medial meniscal tear (p = 0.04), and the lateral femoral condyle was noted to be significantly longer than the lateral tibial plateau in the sagittal plane in patients with a lateral meniscal tear (p
Published: 2019

27. Evaluation of agreement between hemoglobin A1c, fasting glucose, and fructosamine in Senegalese individuals with and without sickle-cell trait

Author: Sarah Skinner, Mor Diaw, Maïmouna Ndour Mbaye, Philippe Joly, Céline Renoux, Céline Masson, Charlotte Cuerq, Philomène Lopez, Malick Ndour, Demba Diedhiou, Djiby Sow, Saliou Diop, Abdoulaye Samb, Vincent Pialoux, Philippe Connes, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Hôpital Principal de Dakar, Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Laboratoire de Biochimie et Toxicologie [Centre Hospitalier Lyon Sud - HCL], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Faculté de Médecine, de Pharmacie et d’Odontostomatologie [Dakar, Sénégal] (FMPOS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Blood Glucose, Male, HbA1c, Endocrine Disorders, Science, Biochemistry, Sickle Cell Trait, Geographical Locations, [SCCO]Cognitive science, Endocrinology, Diabetes diagnosis and management, Diabetes Mellitus, Humans, Hemoglobin, ComputingMilieux_MISCELLANEOUS, Glycated Hemoglobin, Medicine and health sciences, Biology and life sciences, Proteins, Fasting, Middle Aged, Senegal, Diagnostic medicine, Type 2 Diabetes, Metabolic Disorders, Hyperglycemia, People and Places, Africa, Fructosamine, Medicine, Female, Biomarkers, Research Article
Abstract: Fasting glucose (FG) and glycated hemoglobin A1c (HbA1c) perform sub-optimally in people of African origin, especially in individuals with sickle-cell trait (SCT). The purpose of this study was to compare the relationships between HbA1c, FG, and fructosamine in individuals from Senegal with and without SCT. HbA1c, FG, and fructosamine were measured in 203 adults from Senegal (100 control: 45 with type 2 diabetes (T2D); 103 SCT: 51 with T2D). Significant, positive correlations were observed between HbA1c and FG, fructosamine and FG, and fructosamine and HbA1c in both groups. The limits of agreement were inappropriately large in both groups for the Bland-Altman plots of HbA1c and FG (control: -95.97 to 83.97%; SCT: -115.9 to 91.52%), fructosamine and FG (control: -100.6 to 99.89%; SCT: -105.6 to 100.6%), and fructosamine and HbA1c (control: -52.03 to 38.98%; SCT: -88.04 to 71.41%). In both groups, the greatest proportion of subjects were considered above the clinical cut-point for hyperglycemia when fructosamine was used as the criterion (control: 33%; SCT: 44.6%), and the lowest percentage of subjects were classified as over the clinical cut-point when HbA1c was used as the criterion (control: 21%; SCT: 27.7%).Substantial disparities between HbA1c, FG, and fructosamine were observed in both groups, and these differences were exaggerated in the SCT group. Therefore, these three biomarkers should not be considered to be interchangeable measures of glycemic control. These biomarkers should be used thoughtfully, and special care should be taken when using them in individuals with SCT.
Published: 2019

28. Automatic depletion with Spectra Optia allows a safe 16% reduction of red blood cell pack consumption in exchanged sickle cell anemia patients

Author: Kamila Kebaili, Olivier Hequet, Alexandra Gauthier, Yves Bertrand, Solène Poutrel, A. Guironnet‐Paquet, Y. Chelghoum, M. Raba, Arnaud Hot, Giovanna Cannas, Jean-François Nicolas, Philippe Connes, Daniela Revesz, Céline Renoux, Marc Vocanson, Fabrice Cognasse, Philippe Joly, Engineering Service, Edouard Herriot Hospital, Service de Médecine Interne [Hôpital Edouard Herriot - HCL], Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS), Service d'hématologie : Immuno-Hématologie pédiatrique et transplantation de moelle osseuse, Hôpital Debrousse, Hospices Civils de Lyon (HCL), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire national des champs magnétiques intenses - Grenoble [2016-2019] (LNCMI-G [2016-2019]), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Service de Médecine Interne, Laboratoire Aimé Cotton (LAC), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire national des champs magnétiques intenses - Grenoble (LNCMI), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UPS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Institut d'hématologie et oncologie pédiatriques, 69008 Lyon, France, parent, Immunologie de l'allergie cutanée et vaccination – Immunology of skin allergy and vaccination, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Institut d'hématologie et d'oncologie pédiatrique [CHU - HCL] (IHOPe), Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Université Jean Monnet [Saint-Étienne] (UJM), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Jean Monnet - Saint-Étienne (UJM), and Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3)
Subjects: Adult, Male, Erythrocytes, Adolescent, Anemia, [SDV]Life Sciences [q-bio], Immunology, Blood volume, Anemia, Sickle Cell, 030204 cardiovascular system & hematology, Hematocrit, 03 medical and health sciences, Young Adult, [SCCO]Cognitive science, 0302 clinical medicine, Heart rate, medicine, Immunology and Allergy, Humans, Prospective Studies, Adverse effect, Child, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, business.industry, Hematology, medicine.disease, Sickle cell anemia, 3. Good health, Red blood cell, medicine.anatomical_structure, Blood pressure, Anesthesia, Female, business, Erythrocyte Transfusion, 030215 immunology
Abstract: Background Chronic red blood cell exchanges (RBCXs) are frequently used to prevent complications in patients with sickle cell anemia, but the scarcity of matched red blood cell packs (RBCPs) is a serious concern. The main goal of this study was to compare the number of RBCPs used during RBCXs between the Spectra Optia (SO) device (with the automatic depletion step) and the former Cobe Spectra (CSP) device. Study design and methods The performances and safety of 300 SO sessions using the automatic depletion step (SO/DE) in 50 patients with sickle cell anemia under a chronic transfusion program over a 1-year period were prospectively analyzed. The numbers of RBCPs saved using this protocol compared to the SO device without depletion and to the CSP device were determined. Results The SO/DE protocol appeared to be safe, as only 5% and 17% of the sessions were characterized by a significant decrease in blood pressure and increase in heart rate (grade 2 adverse events), respectively. Postapheresis hematocrit and fraction of cells remaining reached expected values. The SO/DE protocol required 16% fewer RBCPs compared to SO without depletion, allowing a mean saving of 12 RBCPs per patient and per year and 13% fewer compared to CSP device. Interestingly, the saving was more important for patients with high total blood volume and/or high preapheresis hematocrit. Conclusion The SO/DE protocol is an efficient, safe and cost-effective procedure for patients with sickle cell anemia under a chronic transfusion program.
Published: 2019

29. Transient liver injury and severe neonatal cholestasis in infant with glucose-6-phosphate dehydrogenase deficiency due to a new mutation

Author: P. Joly, Dominique Plantaz, S. Collardeau-Frachon, D. Adjaoud, N. Thomassin, C. Barro, D. Ben Fredj, Laboratoire d'Hématologie, CHU Grenoble, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: Genetic Markers, Male, medicine.medical_specialty, congenital, hereditary, and neonatal diseases and abnormalities, [SDV]Life Sciences [q-bio], Dehydrogenase, macromolecular substances, Gene mutation, Glucosephosphate Dehydrogenase, Pediatrics, Hemolysis, 03 medical and health sciences, g6pd mutations, 0302 clinical medicine, Cholestasis, 030225 pediatrics, Internal medicine, hemic and lymphatic diseases, G6PD deficiency, parasitic diseases, medicine, Hepatic Insufficiency, Humans, Neonatal cholestasis, Hyperbilirubinemia, Liver injury, business.industry, New mutation, Infant, Newborn, Liver insufficiency, nutritional and metabolic diseases, medicine.disease, 3. Good health, Endocrinology, Glucosephosphate Dehydrogenase Deficiency, Pediatrics, Perinatology and Child Health, Mutation (genetic algorithm), Mutation, business, Glucose-6-phosphate dehydrogenase deficiency
Abstract: We report the case of a neonate with a new, previously undescribed, glucose-6-phosphate dehydrogenase (G6PD) gene mutation, which was revealed by severe cholestasis, hyperbilirubinemia, and transient liver dysfunction. The severity of the clinical phenotype with ongoing chronic hemolytic anemia suggests that this mutation belongs to class 1 G6PD deficiency. The hemizygous mutation "c.675G\textgreaterc; p.Trp225Cys" was detected by genomic sequencing. Since severe G6PD deficiency can be revealed by cholestasis, it is important to check G6PD enzyme activity when faced with a case of liver dysfunction in the neonatal period. (C) 2019 Published by Elsevier Masson SAS on behalf of French Society of Pediatrics.
Published: 2019

30. New insights into red cell rheology and adhesion in patients with sickle cell anaemia during vaso‐occlusive crises

Author: Jacques Elion, Marie-Dominique Hardy-Dessources, Sara El Hoss, Régine Hierso, Nathalie Lemonne, Keyne Charlot, Philippe Connes, Claudine Lapoumeroulie, Marc Romana, Caroline Le Van Kim, Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Transfusion Sanguine [Paris] (INTS), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Adaptations au Climat Tropical, Exercice et Santé (ACTES), Université des Antilles et de la Guyane (UAG), Unité Transversale de la Drépanocytose, CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Hôpital Ricou, Insern, UMR 763, Hôpital Robert Debré, Université Paris Diderot - Paris 7 (UPD7), Institut National de la Transfusion Sanguine [Paris] (INTS), Pharmacogénétique et abords thérapeutiques des maladies héréditaires, Université des Antilles et de la Guyane (UAG)-Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Adaptation, Climat Tropical, Exercice et Santé (ACTES), Université des Antilles (UA), Protéines de la membrane érythrocytaire et homologues non-érythroides, and Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adult, Male, medicine.medical_specialty, Erythrocytes, Adolescent, Endothelium, Anemia, sickle cell anaemia, Anemia, Sickle Cell, irreversibly sickled cells, Microcirculation, Young Adult, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Internal medicine, Cell Adhesion, medicine, Humans, Cell adhesion, ComputingMilieux_MISCELLANEOUS, blood rheology, vaso-occlusive crisis, Red Cell, business.industry, hemic and immune systems, Hematology, medicine.disease, adhesion, Red blood cell, medicine.anatomical_structure, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, 030220 oncology & carcinogenesis, Cardiology, Female, Rheology, Complication, business, Vaso-occlusive crisis, 030215 immunology
Abstract: International audience; Painful vaso-occlusive crisis (VOC) represents the most frequent complication encountered by SCA patients. In animal models, increased red blood cell (RBC) adhesion to the endothelium has been demonstrated to initiate VOC by increasing the transit time of RBC through the microcirculation, predisposing them to sickle and occlude the vessels (Kaul et al.
Published: 2018

31. Moderate-intensity endurance-exercise training in patients with sickle-cell disease without severe chronic complications (EXDRE): an open-label randomised controlled trial

Author: Thomas Rupp, Jean-François Grenot, Jean-Benoît Arlet, Barnabas Gellen, Noemie Delrieux, Jugurtha Berkenou, Angèle N. Merlet, Pablo Bartolucci, Jean-Antoine Ribeil, Emma Guillet, Roxane Lacroix, S. Mattioni, Justine Gellen-Dautremer, Frédéric Galactéros, José Mira, Arthur Peyrard, Laurent Messonnier, Christophe Hourdé, Cyril Martin, Anoosha Habibi, François Lionnet, Gaetana Di Liberto, Arnauld Garcin, Léonard Féasson, Etienne Audureau, Sandrine Peyrot, Polyclinique de Poitiers, Laboratory of Exercise Physiology, University of Savoie, Chambery, and University Jean Monnet of Saint-Etienne, Saint-Etienne, Laboratory of Exercise Physiology, Unité des maladies génétiques du globule rouge, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Centre de référence des syndromes drépanocytaires majeurs-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité INSERM 955, Equipe 2, Centre de Référence des Syndromes Drépanocytaires Majeurs, Université Grenoble Alpes - UFR de Langues étrangères (LLCE et LEA) (UGA UFR LLCE LEA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Cytokines, hématopoïèse et réponse immune (CHRI), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Biologie, Ingénierie et Imagerie de la Greffe de Cornée (EA 2521, JE2521, IFR143), Université Jean Monnet [Saint-Étienne] (UJM), La grossesse normale et pathologique: développement et fonctions du placenta et de l'utérus (UMR_S 767), Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Centre de référence des syndromes drépanocytaires majeurs-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri-Mondor, Université Grenoble Alpes (UGA), CHU Tenon [APHP], Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adult, Male, medicine.medical_specialty, Anemia, [SDV]Life Sciences [q-bio], Anemia, Sickle Cell, 030204 cardiovascular system & hematology, Chest pain, law.invention, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, Endurance training, law, Internal medicine, medicine, Humans, Adverse effect, ComputingMilieux_MISCELLANEOUS, Pregnancy, business.industry, Hazard ratio, Hematology, medicine.disease, Acute chest syndrome, 3. Good health, Endurance Training, Treatment Outcome, 030220 oncology & carcinogenesis, Female, medicine.symptom, business
Abstract: Summary Background Exercise could be a triggering factor for vaso-occlusive crises in patients with sickle-cell disease. We aimed to investigate whether a patient-adapted training programme of moderate endurance exercise could be safe and beneficial for patients with sickle-cell disease. Methods We did a multicentre, prospective, open-label, randomised controlled trial at four university hospitals in France. Eligible patients were older than 18 years, with an HbSS or S/β0-thalassaemia genotype, and with no severe chronic complications. All patients underwent cardiopulmonary exercise tests (CPETs) on a stationary bicycle with cardiac, pulmonary, laboratory, and muscle parameter evaluations at the start and end of the study period. We randomly assigned patients (1:1) to the training group (three 45-min exercise sessions per week, for 8 weeks) or the control group (no lifestyle changes) using a central computer-generated randomisation list. During baseline evaluation, patients and researchers were masked to group assignment; randomisation was done after completion of the baseline evaluation to minimise bias. The primary outcome was difference in power output at a blood lactate concentration of 4 mmol/L during CPET between baseline and the end of the 8-week training period. Patients were analysed on a per-protocol basis, excluding those who missed more than 20% of the training sessions or had other major protocol violations. This trial is registered with ClinicalTrials.gov, number NCT02571088, and is completed. Findings Between Sept 8, 2014, and Dec 11, 2015, 40 patients were enrolled (20 to each group). After exclusion of seven patients (one pregnancy, one appendicitis, one protocol violation, two lost to follow-up, and two incompatibilities with work schedule), 33 patients were analysed (15 in the training group, 18 in the control group). At the 8-week follow-up, the absolute change from baseline in mean power output at 4 mmol/L blood lactate was 7·2 W (SD 8·7) in trained patients (from 70·4 W [SD 16·2] at baseline to 77·6 W [15·1] at end of intervention) compared with −0·3 W (9·4) in controls (from 66·2 W [13·8] to 65·9 W [15·6]; mean difference between groups 7·3 W [95% CI 0·7–13·8], p=0·031). No adverse events requiring hospital admission occurred in the training group, whereas five occurred in the control group: four (20%) vaso-occlusive crises (one complicated by an acute chest syndrome), and one (6%) viral infection with isolated chest pain (hazard ratio 0·143 [95% CI 0·024–0·827; p=0·029). Interpretation Moderate-intensity endurance-exercise training seems to be safe for adults with sickle-cell disease without severe chronic complications and significantly improved their functional capacity, especially for exercise levels close to those needed for daily activities. Our findings support consideration of endurance-exercise training as a novel therapeutic strategy for patients with sickle-cell disease. Funding Societe Francaise de Cardiologie and Institut National du Sport, de l'Expertise et de la Performance (INSEP).
Published: 2018

32. Lactate recovery kinetics in response to high-intensity exercises

Author: Roger Oullion, Carine Bret, Pascal Edouard, Hubert Freund, Benjamin Chatel, Laurent Messonnier, Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Laboratoire d'Ingéniérie et Science des Matériaux - EA 4695 (LISM), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Performance, Santé, Métrologie, Société - EA 7507 (PSMS), Université de Reims Champagne-Ardenne (URCA), CHU Saint-Etienne, Laboratory of Exercise Physiology, University of Savoie, Chambery, and University Jean Monnet of Saint-Etienne, Saint-Etienne, Laboratory of Exercise Physiology, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Laboratoire de Physiologie de l'Exercice (LPE), and Université Jean Monnet - Saint-Étienne (UJM)
Subjects: Male, medicine.medical_specialty, Metabolic Clearance Rate, Physiology, [SDV]Life Sciences [q-bio], Physical Exertion, Kinetics, Passive recovery, Models, Biological, Running, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Animal science, Physiology (medical), medicine, Blood lactate, Humans, Computer Simulation, Orthopedics and Sports Medicine, Lactic Acid, Muscle, Skeletal, Exercise, ComputingMilieux_MISCELLANEOUS, Chemistry, High intensity, Public Health, Environmental and Occupational Health, 030229 sport sciences, General Medicine, Human physiology, Lactic acid, Surgery, Time function, Physical Endurance, Whole body, 030217 neurology & neurosurgery
Abstract: The aim of this study was to investigate lactate recovery kinetics after high-intensity exercises. Six competitive middle-distance runners performed 500-, 1000-, and 1500-m trials at 90 % of their current maximal speed over 1500 m. Each event was followed by a passive recovery to obtain blood lactate recovery curves (BLRC). BLRC were fitted by the bi-exponential time function: La(t) = La(0) + A 1(1–e −γ1t ) + A 2(1–e −γ2t ), where La(0) is the blood lactate concentration at exercise completion, and γ 1 and γ 2 enlighten the lactate exchange ability between the previously active muscles and the blood and the overall lactate removal ability, respectively. Applications of the model provided parameters related to lactate release, removal and accumulation rates at exercise completion, and net amount of lactate released during recovery. The increase of running distance was accompanied by (1) a continuous decrease in γ 1 (p
Published: 2016

33. Intrasession and Intersession Reliability of Running Mechanics During Treadmill Sprints

Author: Grégoire P. Millet, Olivier Girard, Franck Brocherie, Jean-Benoit Morin, Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland, French Institute of Sport (INSEP), Research Department, Laboratory Sport, Expertise and Performance (EA7370) (SEP (EA7370)), Institut national du sport, de l'expertise et de la performance (INSEP), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Male, medicine.medical_specialty, Intraclass correlation, Physical Therapy, Sports Therapy and Rehabilitation, Athletic Performance, Instrumented treadmill, Running, 03 medical and health sciences, 0302 clinical medicine, medicine, sprint performance, Humans, Orthopedics and Sports Medicine, Treadmill, reproducibility, Reliability (statistics), Mathematics, [SHS.SPORT]Humanities and Social Sciences/Sport, [SHS.SPORT.AGS]Humanities and Social Sciences/Sport/Sport gesture analysis, within session, Reproducibility of Results, 030229 sport sciences, Mechanics, between sessions, Biomechanical Phenomena, kinematics, kinetics, Exercise Test, Physical therapy, 030217 neurology & neurosurgery
Abstract: Purpose:To determine the intrasession and intersession (ie, within- and between-days) reliability in treadmill sprinting-performance outcomes and associated running mechanics.Methods:After familiarization, 13 male recreational sportsmen (team- and racket-sport background) performed three 5-s sprints on an instrumented treadmill with 2 min recovery on 3 different days, 5–7 d apart. Intrasession (comparison of the 3 sprints of the first session) and intersession (comparison of the average of the 3 sprints across days) reliability of performance, kinetics, kinematics, and spring-mass variables were assessed by intraclass correlation coefficient (ICC) and coefficients of variation (CV%).Results:Intrasession reliability was high (ICC > .94 and CV < 8%). Intersession reliability was good for performance indices (.83 < ICC < .89 and CV < 10%, yet with larger variability for mean velocity than for distance covered or propulsive power) and kinetic parameters (ICC > .94 and CV < 5%, yet with larger variability for mean horizontal forces than for mean vertical forces) and ranged from good to high for all kinematic (.88 < ICC < .95 and CV ≤ 3.5%) and spring-mass variables (.86 < ICC < .99 and CV ≤ 6.5%). Compared with intrasession, minimal detectable differences were on average twice larger for intersession designs, except for sprint kinetics.Conclusion:Instrumented treadmill sprint offers a reliable method of assessing running mechanics during single sprints either within the same session or between days.
Published: 2016

34. The neuromuscular fatigue induced by repeated scrums generates instability that can be limited by appropriate recovery

Author: Baptiste Morel, Christophe Hautier, Sol Agro et hydrosystème Spatialisation (SAS), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Male, medicine.medical_specialty, [SDV]Life Sciences [q-bio], Football, Passive recovery, Physical Therapy, Sports Therapy and Rehabilitation, Electromyography, Standard deviation, Quadriceps Muscle, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Humans, Medicine, Knee, Orthopedics and Sports Medicine, Muscle Strength, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Knee extensors, medicine.diagnostic_test, Muscle fatigue, business.industry, Biomechanics, 030229 sport sciences, Sagittal plane, medicine.anatomical_structure, Neuromuscular fatigue, Athletes, Muscle Fatigue, Physical therapy, business, 030217 neurology & neurosurgery
Abstract: The aim of this study was to evaluate the influence of the fatigue on the machine scrum pushing sagittal forces during repeated scrums and to determine the origin of the knee extensor fatigue. Twelve elite U23 rugby union front row players performed six 6-s scrums every 30 s against a dynamic scrum machine with passive or active recovery. The peak, average, and the standard deviation of the force were measured. A neuromuscular testing procedure of the knee extensors was carried out before and immediately after the repeated scrum protocol including maximal voluntary force, evoked force, and voluntary activation. The average and peak forces did not decrease after six scrums with passive recovery. The standard deviation of the force increased by 70.2 ± 42.7% (P
Published: 2016

35. Impaired oxygen uptake efficiency slope and off-transient kinetics of pulmonary oxygen uptake in sickle cell anemia are associated with hemorheological abnormalities

Author: Philippe Connes, Valérie Soter, Marie-Dominique Hardy-Dessources, Olivier Hue, Stéphane Sinnapah, Maryse Etienne-Julan, Nathalie Lemonne, Keyne Charlot, Mona Hedreville, Xavier Waltz, Adaptation, Climat Tropical, Exercice et Santé (ACTES), Université des Antilles (UA), Unité Transversale de la Drépanocytose, CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Hôpital Ricou, Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pointe-à-Pitre/Abymes [Guadeloupe], Université des Antilles (Pôle Guadeloupe), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Male, medicine.medical_specialty, Physiology, Anemia, [SDV]Life Sciences [q-bio], Physical fitness, Population, Hematocrit, Incremental exercise, Oxygen Consumption, Erythrocyte Deformability, Physiology (medical), Internal medicine, medicine, Humans, education, hemorheology, education.field_of_study, medicine.diagnostic_test, Physical activity, business.industry, exercise recovery, Hematology, Middle Aged, medicine.disease, Sickle cell anemia, 3. Good health, Kinetics, Immunology, physical fitness, Cardiology, Female, sickle cell disease, Hemorheology, Cardiology and Cardiovascular Medicine, Ventilatory threshold, business
Abstract: International audience; Oxygen uptake efficiency slope (OUES) and excess post-exercise oxygen consumption (EPOC) are markers of physical fitness in the general population but have never been characterized in sickle cell anemia (SCA) where hematological and hemorheological properties are severely altered. Eight SCA patients and eleven healthy subjects (CONT) performed a submaximal incremental exercise conducted until the first ventilatory threshold (VT1). OUES was calculated from the data collected during the incremental period and EPOC parameters (amplitude [A] and time constant [τ]) were calculated from the data measured during exercise recovery. We found that OUES (p = 0.007) and A (p = 0.010) were lower, and τ (p = 0.035) was higher, in SCA patients compared to CONT subjects. OUES and τ were significantly correlated with hematocrit, red blood cell (RBC) deformability and RBC aggregates strength. Our findings suggest that both the abilities to use oxygen during exercise and to recover after a physical activity are impaired in SCA patients. This poor physical fitness seems to depend on the degree of anemia and RBC rheological alterations.
Published: 2015

36. Inflammatory Response 24 h Post-Exercise in Youth with Juvenile Idiopathic Arthritis

Author: Christophe Hourdé, Stéphane Echaubard, Emmanuelle Rochette, Pascale Duché, Etienne Merlin, Bruno Peraira, Bertrand Evrard, CHU Clermont-Ferrand, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Impact de l'Activité Physique sur la Santé (IAPS), Université de Toulon (UTLN), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, Time Factors, [SDV]Life Sciences [q-bio], Arthritis, Pituitary-Adrenal System, Systemic inflammation, Gastroenterology, [SHS]Humanities and Social Sciences, 0302 clinical medicine, Cytokine Receptor gp130, Medicine, Orthopedics and Sports Medicine, Child, ComputingMilieux_MISCELLANEOUS, Pain Measurement, biology, Venous blood, Disease Progression, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, medicine.symptom, medicine.medical_specialty, Hypothalamo-Hypophyseal System, Adolescent, Physical Therapy, Sports Therapy and Rehabilitation, Proinflammatory cytokine, 03 medical and health sciences, Adrenocorticotropic Hormone, Internal medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Juvenile, Humans, Exercise physiology, Interleukin 6, Exercise, 030203 arthritis & rheumatology, [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics, business.industry, Interleukin-6, 030229 sport sciences, Dehydroepiandrosterone, medicine.disease, Receptors, Interleukin-6, Arthritis, Juvenile, biology.protein, Calprotectin, business, Leukocyte L1 Antigen Complex, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: The aim of this study was to measure the impact, at 24 h post-exercise, of a single exercise bout on plasma inflammatory markers such as calprotectin, IL-6, sIL-6 R, sgp130 and the hypothalamic-pituitary-adrenal (HPA) axis in children with juvenile idiopathic arthritis (JIA).Twelve children with JIA attended the laboratory on three consecutive days (control day, exercise day and 24 h post-exercise), including a 20-min exercise bout on a cycle-ergometer at 70% of max. HR at 8:30 a.m. on day 2. Plasma concentrations of calprotectin, IL-6, sIL-6 R, sgp130, cortisol, ACTH and DHEA were measured on venous blood samples taken every day.at rest and at 8:30, 8:50, 9:30, 10:30 a.m. and 12:00, 3:00, 5:30 p.m.A single exercise bout increased plasma calprotectin 1.7-fold (pPhysical activity in children with JIA results in a slight transient systemic inflammation but seems to be followed by counter-regulation at 24 h post-exercise with a decrease in proinflammatory markers.
Published: 2018

37. Implementing Cognitive Training Into a Surgical Skill Course: A Pilot Study on Laparoscopic Suturing and Knot Tying

Author: Franck Di Rienzo, Nady Hoyek, Xavier Martin, Ye Haixia, Benjamin De Witte, Christian Collet, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Hôpital Edouard Herriot [CHU - HCL], and Hospices Civils de Lyon (HCL)
Subjects: Adult, Male, [SDV]Life Sciences [q-bio], education, Pilot Projects, Visual feedback, Workload, Space (commercial competition), Course (navigation), [SCCO]Cognitive science, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Motor imagery, Cognition, Human–computer interaction, Surgical skills, Medicine, Humans, ComputingMilieux_MISCELLANEOUS, Haptic technology, Cognitive Behavioral Therapy, business.industry, Suture Techniques, Internship and Residency, Cognitive training, Knot tying, Motor Skills, 030220 oncology & carcinogenesis, Surgery, Female, Laparoscopy, Clinical Competence, business, 030217 neurology & neurosurgery, Psychomotor Performance
Abstract: Mini-invasive surgery—for example, laparoscopy—has challenged surgeons’ skills by extending their usual haptic space and displaying indirect visual feedback through a screen. This may require new mental abilities, including spatial orientation and mental representation. This study aimed to test the effect of cognitive training based on motor imagery (MI) and action observation (AO) on surgical skills. A total of 28 postgraduate residents in surgery took part in our study and were randomly distributed into 1 of the 3 following groups: (1) the basic surgical skill, which is a short 2-day laparoscopic course + MI + AO group; (2) the basic surgical skill group; and (3) the control group. The MI + AO group underwent additional cognitive training, whereas the basic surgical skill group performed neutral activity during the same time. The laparoscopic suturing and knot tying performance as well as spatial ability and mental workload were assessed before and after the training period. We did not observe an effect of cognitive training on the laparoscopic performance. However, the basic surgical skill group significantly improved spatial orientation performance and rated lower mental workload, whereas the 2 others exhibited lower performance in a mental rotation test. Thus, actual and cognitive training pooled together during a short training period elicited too high a strain, thus limiting potential improvements. Because MI and AO already showed positive outcomes on surgical skills, this issue may, thus, be mitigated according to our specific learning conditions. Distributed learning may possibly better divide and share the strain associated with new surgical skills learning.
Published: 2018

38. Do aerobic characteristics explain isometric exercise-induced neuromuscular fatigue and recovery in upper and lower limbs?

Author: Gianluca Vernillo, Guillaume Y. Millet, Matthieu Martin, John Temesi, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Imagerie Ultrasonore, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adult, Male, medicine.medical_specialty, Peripheral nerve stimulation, Elbow, 030209 endocrinology & metabolism, Physical Therapy, Sports Therapy and Rehabilitation, Isometric exercise, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Physical medicine and rehabilitation, Oxygen Consumption, Isometric Contraction, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], medicine, Humans, Orthopedics and Sports Medicine, Knee, Power output, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Muscle fatigue, business.industry, Electromyography, VO2 max, 030229 sport sciences, Evoked Potentials, Motor, Transcranial Magnetic Stimulation, Peripheral, medicine.anatomical_structure, Neuromuscular fatigue, Muscle Fatigue, Exercise Test, business
Abstract: This study investigated the relationships between aerobic characteristics and (i) neuromuscular fatigue induced by 2-min sustained isometric maximal voluntary contractions (MVC) and (ii) subsequent recovery, in the upper and lower limbs. In a pseudo-randomized order, eleven healthy males completed four sessions on different days: maximal incremental cycling test (100 W + 40 W every 2 min); maximal arm-cranking test (50 W + 20 W every 2 min); and 2-min sustained isometric MVCs of the knee extensors (KE) and elbow flexors (EF). Neuromuscular assessment was performed with transcranial magnetic and peripheral nerve stimulation to evaluate central and peripheral neuromuscular factors of fatigue and the subsequent recovery. Peak oxygen uptake, gas exchange threshold and the corresponding power outputs were correlated with recovery of voluntary force after the 2-min KE MVC. Regression analysis showed that power output at the gas exchange threshold alone explained 72% of the variability in ∆recovery of KE voluntary force. No relationships with fatigue or recovery in EF were observed. These results suggest that participants with greater aerobic capacities experience the same amount of fatigue and faster recovery of voluntary force in KE but not EF. The potential reasons behind the relationship in KE but not EF are discussed.
Published: 2018

39. Pulse wave velocity is lower in trained than in untrained sickle cell trait carriers

Author: Abdou Khadir Sow, Abdoulaye Samb, Mor Diaw, M. Hallab, Valentin Ouedraogo, Mountaga Diop, Arnaud Jean Florent Tiendrebeogo, Rhonda BeLue, Georges Leftheriotis, Moussa Seck, Abdoulaye Ba, Philippe Connes, Julien Tripette, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Laboratory of Biorheology and Medical Ultrasonics (LBUM), Université de Montréal (UdeM)-Hospital Research Center, Laboratoire de Physiologie, Faculté de Médecine et de Pharmacie, Université de Nantes (UN), Laboratory of Chemistry, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), and Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Adult, Male, Sickle cell trait, medicine.medical_specialty, Benign condition, Adolescent, Physiology, pulse wave velocity, [SDV]Life Sciences [q-bio], Blood viscosity, Population, Physical exercise, Pulse Wave Analysis, 030204 cardiovascular system & hematology, Young Adult, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, Physiology (medical), Internal medicine, hemic and lymphatic diseases, medicine, Humans, education, Pulse wave velocity, education.field_of_study, business.industry, 030229 sport sciences, Hematology, medicine.disease, surgical procedures, operative, Cardiology, Arterial stiffness, blood viscosity, Population study, Female, Cardiology and Cardiovascular Medicine, business, therapeutics, human activities, exercise training
Abstract: BACKGROUND Sickle cell trait (SCT) is a benign condition of sickle cell disease. Nevertheless, previous reports showed that SCT carriers have increased blood viscosity and decreased vascular reactivity compared to non-SCT carrier. The benefit of regular exercise on vascular function has been well documented in the general population but no study focused on the SCT population. PURPOSE The aim of our study was to compare arterial stiffness and blood viscosity between trained and untrained SCT carriers, as well as a group of untrained non-SCT. METHODS Arterial stiffness (finger-toe pulse wave velocity) and blood viscosity were evaluated in untrained non-SCT carriers (n = 10), untrained SCT carriers (n = 23) and trained SCT carriers (n = 17) who reported at least 10 hours of physical exercise per week. RESULTS Untrained SCT carriers had higher pulse wave velocity (p = 0.032) and blood viscosity (p
Published: 2018

40. Eryptosis and hemorheological responses to maximal exercise in athletes: Comparison between running and cycling

Author: Ivan Hancco, Philippe Connes, Céline Renoux, Nicolas Guillot, R. Fort, L. Lavorel, Elie Nader, Philippe Joly, Marie Germain, Emeric Stauffer, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire de Médecine Respiratoire [HCL - Hôpital de la Croix Rousse, Lyon], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Service de Médecine Interne [Hôpital Croix Rousse, CHU Lyon], CHU Lyon-Hôpital de la Croix-Rousse [CHU - HCL], Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: Blood Glucose, Male, [SDV]Life Sciences [q-bio], Blood viscosity, Hemodynamics, 030204 cardiovascular system & hematology, Hematocrit, hemodynamics, Running, Hypoxemia, 0302 clinical medicine, eryptosis, Erythrocyte deformability, Orthopedics and Sports Medicine, deformability, Treadmill, Hypoxia, ComputingMilieux_MISCELLANEOUS, mechanisms, medicine.diagnostic_test, exercise, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, red blood cell deformability, medicine.anatomical_structure, Female, rheology, medicine.symptom, Adult, medicine.medical_specialty, hematocrit, Physical Therapy, Sports Therapy and Rehabilitation, Phosphatidylserines, 03 medical and health sciences, Oxygen Consumption, Erythrocyte Deformability, Internal medicine, medicine, Humans, red-blood-cells, Lactic Acid, relevance, arterial hypoxemia, business.industry, sickle-cell-anemia, 030229 sport sciences, Hypoxia (medical), Bicycling, Red blood cell, Endocrinology, viscosity, blood viscosity, Calcium, Reactive Oxygen Species, business, Sport Sciences
Abstract: International audience; We compared the effects of cycling and running exercise on hemorheological and hematological properties, as well as eryptosis markers. Seven endurance-trained subjects randomly performed a progressive and maximal exercise test on a cycle ergometer and a treadmill. Blood was sampled at rest and at the end of the exercise to analyze hematological and blood rheological parameters including hematocrit (Hct), red blood cell (RBC) deformability, aggregation, and blood viscosity. Hemoglobin saturation (SpO2), blood lactate, and glucose levels were also monitored. Red blood cell oxidative stress, calcium content, and phosphatidylserine exposure were determined by flow cytometry to assess eryptosis level. Cycling exercise increased blood viscosity and RBC aggregation whereas it had no significant effect on RBC deformability. In contrast, blood viscosity remained unchanged and RBC deformability increased with running. The increase in Hct, lactate, and glucose concentrations and the loss of weight at the end of exercise were not different between running and cycling. Eryptosis markers were not affected by exercise. A significant drop in SpO2 was noted during running but not during cycling. Our study showed that a progressive and maximal exercise test conducted on a cycle ergometer increased blood viscosity while the same test conducted on a treadmill did not change this parameter because of different RBC rheological behavior between the 2 tests. We also demonstrated that a short maximal exercise does not alter RBC physiology in trained athletes. We suspect that exercise-induced hypoxemia occurring during running could be at the origin of the RBC rheological behavior differences with cycling.
Published: 2018

41. Dual mobility cups in total hip arthroplasty after failed internal fixation of proximal femoral fractures

Author: Frédéric Farizon, Sandrine Boulat, Rémi Philippot, Bertrand Boyer, Thomas Neri, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Biologie intégrative du tissu osseux, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM), Centre d'Orthopédie et Traumatologie (COT), and CHU Saint-Etienne
Subjects: musculoskeletal diseases, Adult, Male, Reoperation, medicine.medical_specialty, Radiography, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Arthroplasty, Replacement, Hip, Context (language use), 03 medical and health sciences, Fracture Fixation, Internal, 0302 clinical medicine, medicine, Internal fixation, Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, Treatment Failure, Aged, Retrospective Studies, Aged, 80 and over, 030222 orthopedics, Proximal femur, business.industry, Hip Fractures, Retrospective cohort study, Middle Aged, Dual mobility, Surgery, Prosthesis Failure, Female, Hip Joint, Implant, Hip Prosthesis, business, Total hip arthroplasty
Abstract: Introduction Performing total hip arthroplasty (THA) following failed internal fixation of proximal femur fractures is associated with an elevated risk of implant dislocation. We hypothesized that using a dual mobility (DM) cup will help to reduce the risk of postoperative instability in this specific context. Material and methods This was a retrospective study of 33 consecutive patients who underwent DM THA following failed internal fixation of a proximal femur fracture. The clinical assessment consisted of the Postel-Merle d’Aubigne and HHS scores along with an analysis of preoperative and follow-up radiographs. The primary outcome was the occurrence of implant dislocation. Results At the last follow-up (44 ± 24 months), 7 patients had died and 0 were lost to follow-up. Only one dislocation had occurred (3%). The mean PMA and HSS scores of 14.8 and 80 respectively were significantly better than the preoperative scores. There were no cases of aseptic loosening. Conclusion The use of DM cups in the context of THA following failed internal fixation of proximal femur fractures helps to reduce the risk of dislocation. Thus DM cups are recommended in this indication with high risk of postoperative instability.
Published: 2018

42. Blood rheological abnormalities in sickle cell anemia

Author: Marie-Dominique Hardy-Dessources, Cyril Martin, Philippe Connes, Céline Renoux, Marc Romana, Samir K. Ballas, Manouk Abkarian, Philippe Joly, Adaptation, Climat Tropical, Exercice et Santé (ACTES), Université des Antilles (UA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Biochimie Structurale [Montpellier] (CBS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Fiabilité / Puissance, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Transfusion Sanguine [Paris] (INTS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université des Antilles et de la Guyane (UAG), Laboratory of Excellence GR-Ex 'The red cell: from genesis to death', and PRES Sorbonne Paris Cité
Subjects: Male, medicine.medical_specialty, Physiology, Anemia, [SDV]Life Sciences [q-bio], Priapism, Blood viscosity, Anemia, Sickle Cell, 030204 cardiovascular system & hematology, 03 medical and health sciences, red blood cell aggregation, 0302 clinical medicine, Glomerulopathy, Physiology (medical), Internal medicine, Erythrocyte Deformability, medicine, Erythrocyte deformability, Humans, ComputingMilieux_MISCELLANEOUS, business.industry, hemic and immune systems, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, Hematology, red blood cell deformability, medicine.disease, Sickle cell anemia, Hemolysis, 3. Good health, Red blood cell, medicine.anatomical_structure, Cardiology, blood viscosity, Female, Cardiology and Cardiovascular Medicine, business, Rheology, 030215 immunology
Abstract: International audience; This review focuses on the contribution of abnormal blood rheology in the pathophysiology of sickle cell anemia (SCA). SCA is characterized by a reduction of red blood cell (RBC) deformability but this reduction is very heterogeneous among patients. Recent works have shown that patients with the lowest RBC deformability (measured by ektacytometry) have enhanced hemolysis and would be more prone to develop several complications such as priapism, leg ulcers and glomerulopathy. In contrast, patients with the highest deformability, and not under hydroxyurea therapy, seem to develop more frequently vaso-occlusive like events. Although less studied, RBC aggregation properties are very different between SCA and healthy individuals and it was demonstrated that increased RBC aggregates strength could be involved in some complications. Finally, several studies have established that the vascular system of SCA patients could not fully compensate any increase in blood viscosity because of the loss of vascular reactivity, which may result in vaso-occlusive crises.
Published: 2018

43. Cross sectional study on prevalence of sickle cell alleles S and C among patients with mild malaria in Ivory Coast

Author: Serge-Brice Assi, Baba Coulibaly, Stephane Koui Tossea, David N’golo Coulibaly, Philippe Joly, Eric Gbessi Adji, Berenger Ako Ako, Ronan Jambou, André Offianan Touré, Institut Pasteur de Côte d'Ivoire, Réseau International des Instituts Pasteur (RIIP), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Unité de Pathologie Moléculaire du Globule Rouge, Hospices Civils de Lyon (HCL)-Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Institut Pierre Richet (IPR), Programme National de Lutte Contre le Paludisme [Côte d'Ivoire], Ministère de la Santé [Côte d'Ivoire], Département Parasites et Insectes vecteurs - Department of Parasites and Insect Vectors, Institut Pasteur [Paris], This study was supported by a grant from the International Rotary foundation and the District of Paris Rotary., Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Institut Pasteur [Paris] (IP)
Subjects: Male, Cross-sectional study, [SDV]Life Sciences [q-bio], Hemoglobin, Sickle, lcsh:Medicine, Comorbidity, 0302 clinical medicine, Pregnancy, Genotype, Prevalence, 030212 general & internal medicine, Child, lcsh:QH301-705.5, education.field_of_study, Geography, Hemoglobin C, General Medicine, Sickle cell anemia, 3. Good health, Research Note, Child, Preschool, Female, Ivory Coast, Adult, medicine.medical_specialty, Adolescent, 030231 tropical medicine, Population, Anemia, Sickle Cell, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Young Adult, Internal medicine, parasitic diseases, medicine, Humans, lcsh:Science (General), education, Alleles, Retrospective Studies, business.industry, lcsh:R, Infant, Newborn, Infant, Retrospective cohort study, medicine.disease, Abnormal hemoglobin, Malaria, Cote d'Ivoire, Cross-Sectional Studies, lcsh:Biology (General), Pregnancy Complications, Parasitic, FRET, Hemoglobin, business, lcsh:Q1-390
Abstract: Objectives Sickle cell anemia is due to a mutations on the betaglobin gene, inducing abnormal hemoglobin. In West Africa the main mutations lead to S or C types of hemoglobin. Patients with homozygote mutations seem protected against severe malaria, but not against mild disease. The prevalence of abnormal hemoglobin among patients attending dispensaries for mild malaria is thus unknown. A retrospective study was conducted to update data on the prevalence of S and C hemoglobin among patients attending dispensaries with mild malaria. Enrolment of patients was conducted during in vivo malaria treatment efficacy survey following the 42 days WHO protocol. A group of non-infected pregnant women and a group of patients with fever different from malaria, were also recruited in the same dispensaries. Results 794 blood samples were included. S and C genotypes were found in all the regions of Ivory Coast with the highest prevalence in the Northern region (S and C genotypes, 27%). In non-infected patients, prevalence of mutations was higher than in malaria patients. Conclusion A high proportion of patients with mild malaria carried genetic hemoglobin disorder. This population of high risk must be better investigated to control treatment efficacy and to manage complications. Electronic supplementary material The online version of this article (10.1186/s13104-018-3296-7) contains supplementary material, which is available to authorized users.
Published: 2018

44. Lipid profiles in French West Indies sickle cell disease cohorts, and their general population

Author: Benoît Tressières, Marc Romana, Yann Lamarre, Philippe Connes, Marie-Laure Lalanne-Mistrih, Marie-Dominique Hardy-Dessources, Vanessa Tarer, Nathalie Lemonne, Dominique Mougenel, Maryse Etienne-Julan, Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Unité Transversale de la Drépanocytose [Pointe-à-Pitre, Guadeloupe], CHU Pointe-à-Pitre/Abymes [Guadeloupe], Centre d'investigation clinique Antilles-Guyane (CIC - Antilles Guyane), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Transversale de la Drépanocytose, CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Hôpital Ricou, Centre de référence maladies rares pour la drépanocytose aux Antilles-Guyane, Laboratory of Excellence GR-Ex 'The red cell: from genesis to death', PRES Sorbonne Paris Cité, Université des Antilles et de la Guyane (UAG), and BMC, BMC
Subjects: Male, 0301 basic medicine, Epidemiology, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Clinical Biochemistry, [SDV.GEN] Life Sciences [q-bio]/Genetics, 030204 cardiovascular system & hematology, Gastroenterology, 0302 clinical medicine, Endocrinology, Genotype, Medicine, Guadeloupe, lcsh:RC620-627, Abdominal obesity, ComputingMilieux_MISCELLANEOUS, Body mass index, education.field_of_study, Osteonecrosis, Middle Aged, Lipids, Sickle cell anemia, 3. Good health, Non HDL-cholesterol, [SDV] Life Sciences [q-bio], lcsh:Nutritional diseases. Deficiency diseases, Female, medicine.symptom, Adult, medicine.medical_specialty, Lipoproteins, Population, Anemia, Sickle Cell, Clinical nutrition, Hemolysis, 03 medical and health sciences, Internal medicine, Acute Chest Syndrome, Genetics, Humans, Vascular Diseases, education, Triglycerides, Retrospective Studies, [SDV.GEN]Life Sciences [q-bio]/Genetics, business.industry, Research, Sickle cell disease, Biochemistry (medical), Case-control study, medicine.disease, Acute chest syndrome, 030104 developmental biology, Apolipoproteins, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Case-Control Studies, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business
Abstract: International audience; AbstractBackgroundThe pathophysiology of sickle cell disease (SCD) and the variability of its clinical expression remain not fully understood, whether within or between different SCD genotypes. Recent studies have reported associations between lipid levels and several SCD complications. If lipid levels have been previously described as low in sickle cell anemia (SCA), few data have been provided for sickle cell SC disease (SCC). We designed our epidemiological study to isolate lipid levels and profiles by genotype in Guadeloupian cohorts of SCA and SCC adult patients, at steady state. We compared SCD lipid levels with those of the Guadeloupian general population (GGP), and analyzed potential associations between lipid levels and SCD complications (vaso-occlusive crises, acute chest syndrome and osteonecrosis).MethodsLipids, apolipoproteins, biological variables and anthropometric evaluation, were collected at steady state from medical files for 62 SCC and 97 SCA adult patients. Clinical SCD complications were collected from the clinical files. Analysis was conducted by genotype for all variables.ResultsDifferent SCC and SCA lipid profiles, both distinct from their GGP’s, were identified. Compared to SCC and GGP, higher triglyceride (TG) levels were observed in SCA patients, independent of hydroxyurea, hemolysis, gender, age, body mass index (BMI), abdominal obesity and clinical nutritional status. Our survey highlights also subsequent anthropometrical phenotypes, with an over-representation of abdominal obesity with normal BMI in SCA patients, and affecting almost exclusively females in both genotypes. Moreover, more frequent positive history of acute chest syndrome (ACS) was observed in SCA patients with TG level higher than 1.50 g/l, and of osteonecrosis in SCC patients having non high-density lipoprotein-cholesterol level (Non HDL-C) higher than 1.30 g/l.ConclusionsThis study reveals that SCA and SCC patients exhibit distinct lipid profiles and suggests that high TG and Non HDL-C levels are associated with past histories of ACS and osteonecrosis in SCA and SCC patients, respectively.
Published: 2018

45. Fifteen Minutes Daily Brisk Walk May Be a New Best Target in Very Old Adults: Age Is Not an Excuse to Not Exercise

Author: Remi Bouvier, Bienvenu Bongue, Nathalie Barth, Rémi Poillerat, Jérémy Raffin, Marc Thillays, Thierry Busso, Frédéric Roche, Léonard Féasson, Jean-Claude Barthélémy, Martin Garet, Claude Montuy-Coquard, Amandine Devun, David Hupin, Service de Physiologie Clinique et de l’Exercice, Faculté de Médecine Jacques Lisfranc, Système Nerveux Autonome - Epidémiologie, Physiologie, Ingénierie, Santé (SNA-EPIS), Université Jean Monnet [Saint-Étienne] (UJM)-Centre Hospitalier Universitaire de Saint-Etienne, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Unité de Myologie, Centre Référent Maladies Neuromusculaires Rares Rhône-Alpes, Unité de recherche clinique [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU), Centre Technique d'Appui et de Formation des Centres d'Examens de Santé (CETAF), CETAF, Service de Physiologie Clinique et de l'Exercice, CHU Nord, Faculté de Médecine Jacques Lisfranc, Université Jean Monnet (Saint-Etienne), Laboratoire sur les Vulnérabilités et l'Innovation dans le Sport (EA 7428) (L-VIS), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon
Subjects: Adult, Male, Aging, medicine.medical_specialty, MEDLINE, Walking, 030204 cardiovascular system & hematology, [SHS]Humanities and Social Sciences, 03 medical and health sciences, 0302 clinical medicine, Cause of Death, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, Medicine, 030212 general & internal medicine, Exercise, General Nursing, ComputingMilieux_MISCELLANEOUS, Aged, Cause of death, business.industry, Health Policy, General Medicine, Excuse, Walking Speed, Preferred walking speed, Physical therapy, Geriatrics and Gerontology, business
Abstract: International audience
Published: 2018

46. Intraprosthetic dislocation of dual mobility total hip arthroplasty: still occurring?

Author: Thomas Neri, Jacques H Caton, Alexandre Di Iorio, Frédéric Farizon, Bertrand Boyer, Jean Geringer, Remi PhiIippot, Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F - 42023 Saint-Etienne France, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre d'Orthopédie et Traumatologie (COT), CHU Saint-Etienne, Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Centre Hospitalier Universitaire de Lyon, Service de Chirurgie Orthopédique et Traumatologique, Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre Albert Trillat [Hôpital de la Croix-Rousse - HCL], and Hôpital de la Croix-Rousse [CHU - HCL]
Subjects: Adult, Male, medicine.medical_specialty, Adolescent, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Arthroplasty, Replacement, Hip, Dentistry, Computed tomography, Prosthesis Design, [SPI]Engineering Sciences [physics], 03 medical and health sciences, Young Adult, 0302 clinical medicine, Imaging, Three-Dimensional, medicine, Hip Dislocation, Humans, Orthopedics and Sports Medicine, Range of Motion, Articular, ComputingMilieux_MISCELLANEOUS, Reduction (orthopedic surgery), Device Removal, Aged, 030203 arthritis & rheumatology, Aged, 80 and over, 030222 orthopedics, Insert (composites), medicine.diagnostic_test, business.industry, Middle Aged, bacterial infections and mycoses, Dual mobility, Prosthesis Failure, Orthopedic surgery, Surgery, Female, Hip Prosthesis, Dislocation, business, Tomography, X-Ray Computed, Total hip arthroplasty
Abstract: The objective was to identify predictive factors for intraprosthetic dislocation (IPD) and to understand how improvements in dual mobility cups (DMC) have helped to reduce dramatically the occurrence of this complication. DM mobile inserts retrieved from 93 hips were divided into three groups: first-generation DMC with IPD (“firstDMC-IPD”), first-generation DMC with over 15 years of implantation without IPD (“firstDMC-noIPD”), and latest-generation DMC (“newDMC”). The predictive factors for IPD based on clinical, prosthetic, radiological and intraoperative characteristics were analysed by multivariate analysis. The surface of each retrieved mobile insert was analysed using three-dimensional CT scan in order to compare their rim wear. Three predictive factors for IPD were found: a high BMI, a wide rough stem neck and a large cup size. Wear of the firstDMC-noIPD inserts was significantly less than those of firstDMC-IPD inserts and significantly more than those of newDMC inserts. For the firstDMC-IPD inserts, the rim’s outer surface wear was significantly greater than the rim’s inner surface wear. IPD is a specific complication related to wear of the DM mobile insert due to failure of the liner’s retaining rim, especially from the rim’s outer surface. This long-term issue is different to the early traumatic complication, which can happen after an attempt at closed reduction of a DM THA dislocation. Recent modifications in the design and the coating of contemporary DMC and femoral stems, as well as improvements in the mobile insert itself, seem to corroborate our assumptions about the IPD mechanism and contribute to the quasi-disappearance of this complication.
Published: 2018

47. Long-term wear of dual mobility total hip replacement cups: explant study

Author: Bertrand Boyer, Frédéric Farizon, Alexandre Di Iorio, Rémi Philippot, Jean Geringer, Thomas Neri, Centre d'Orthopédie et Traumatologie (COT), CHU Saint-Etienne, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Centre Hospitalier Universitaire de Lyon, Service de Chirurgie Orthopédique et Traumatologique, Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Ingénierie et Santé (CIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), and Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F - 42023 Saint-Etienne France
Subjects: musculoskeletal diseases, Adult, Male, medicine.medical_specialty, medicine.medical_treatment, Arthroplasty, Replacement, Hip, [SDV]Life Sciences [q-bio], Total hip replacement, Dentistry, Prosthesis Design, Prosthesis, 03 medical and health sciences, [SPI]Engineering Sciences [physics], 0302 clinical medicine, Green led, Medicine, Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, Aged, Retrospective Studies, 030222 orthopedics, business.industry, technology, industry, and agriculture, Level iv, Middle Aged, equipment and supplies, Dual mobility, Surgery, Prosthesis Failure, surgical procedures, operative, Fully automatic, Female, Hip Prosthesis, business, human activities, Total hip arthroplasty
Abstract: Dual mobility THA implantations are increasing. THA survival of more than 15 years is considered long-term survival based on the Charnley prosthesis, which has a median wear rate of 39 to 98 mm3/year. What is the median wear rate of dual mobility THA cups and its repartition on the liner volume? What are the factors influencing dual mobility wear? Thirty-five explants were analysed. The liners were entirely mapped with a fully automatic stripe light scanner that uses green LED light. The liners had a median wear rate of 38 mm3/year. The distribution of the wear between the two bearings varied greatly. No factor was found influencing wear volumes. The median annual wear of dual mobility liners is at least as low as the wear of cemented polyethylene liners and lower than equivalent cementless liners. The two articulations of the dual mobility THA do not cause more wear. Level IV - case series.
Published: 2018

48. Training at maximal power in resisted sprinting: Optimal load determination methodology and pilot results in team sport athletes

Author: Cross, Matt R., Lahti, Johan, Brown, Scott R., Chedati, Mehdi, Jimenez-Reyes, Pedro, Samozino, Pierre, Eriksrud, Ola, Morin, Jean-Benoit, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Côte d'Azur (UCA), Laboratoire Motricité Humaine Expertise Sport Santé (LAMHESS), Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université de Toulon (UTLN)-Université Côte d'Azur (UCA)
Subjects: Adult, Male, velocity, human performance, Physiology, [SDV]Life Sciences [q-bio], education, lcsh:Medicine, Pilot Projects, Athletic Performance, Research and Analysis Methods, Remote Sensing, Weight-Bearing, Motion, Mathematical and Statistical Techniques, Medicine and Health Sciences, running, Humans, Statistical Methods, lcsh:Science, robotics, Behavior, Biological Locomotion, Physics, Mechanical Engineering, lcsh:R, Biology and Life Sciences, Classical Mechanics, Resistance Training, acceleration, Sports Science, Athletes, Physical Sciences, linear regression analysis, Recreation, Regression Analysis, Engineering and Technology, Female, lcsh:Q, sports, human activities, Mathematics, Statistics (Mathematics), Research Article, radar
Abstract: Aims: In the current study we investigated the effects of resisted sprint training on sprinting performance and underlying mechanical parameters (force-velocity-power profile) based on two different training protocols: (i) loads that represented maximum power output (Lopt) and a 50% decrease in maximum unresisted sprinting velocity and (ii) lighter loads that represented a 10% decrease in maximum unresisted sprinting velocity, as drawn from previous research (L10). Methods: Soccer [n = 15 male] and rugby [n = 21; 9 male and 12 female] club-level athletes were individually assessed for horizontal force-velocity and load-velocity profiles using a battery of resisted sprints, sled or robotic resistance respectively. Athletes then performed a 12-session resisted (10 × 20-m; and pre- post-profiling) sprint training intervention following the L10 or Lopt protocol. Results: Both L10 and Lopt training protocols had minor effects on sprinting performance (average of -1.4 to -2.3% split-times respectively), and provided trivial, small and unclear changes in mechanical sprinting parameters. Unexpectedly, Lopt impacted velocity dominant variables to a greater degree than L10 (trivial benefit in maximum velocity; small increase in slope of the force-velocity relationship), while L10 improved force and power dominant metrics (trivial benefit in maximal power; small benefit in maximal effectiveness of ground force orientation). Conclusions: Both resisted-sprint training protocols were likely to improve performance after a short training intervention in already sprint trained athletes. However, widely varied individualised results indicated that adaptations may be dependent on pre-training force-velocity characteristics.
Published: 2018

49. Maternal exercise modifies body composition and energy substrates handling in male offspring fed a high-fat/high-sucrose diet

Author: Quiclet, Charline, Dubouchaud, Herve, Berthon, Phanélie, Sanchez, Hervé, Vial, Guillaume, Siti, Farida, Fontaine, Eric, Batandier, Cécile, Couturier, Karine, Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Institut de Recherche Biomédicale des Armées (IRBA), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), University of Jakarta, Centre Hospitalier Universitaire [Grenoble] (CHU), SFR Sport Exercice Motricité, Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hamant, Sarah, Université Grenoble Alpes - UFR Activités physiques et sportives (UGA UFR APS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Hypoxie : Physiopathologie Respiratoire et Cardiovasculaire (HP2 ), Universitas Indonesia (UI ), and Université Grenoble Alpes (UGA)
Subjects: Male, Sucrose, offspring, [SDV]Life Sciences [q-bio], education, Diet, High-Fat, [SDV] Life Sciences [q-bio], gestation, pancreas, Body Composition, insulin sensitivity, Endocrine, Muscle, Skeletal, metabolism, Exercise, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; Key points: Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood. Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet.Abstract: Maternal exercise during gestation has been reported to modify offspring metabolism and health. Whether these effects are exacerbated when offspring are receiving a high-fat diet remains unclear. Our purpose was to evaluate the effect of maternal exercise before and during gestation on the offspring fed a high-fat/high-sucrose diet (HF) by assessing its body composition, pancreatic function and energy substrates handling by two major glucose-utilizing tissues: liver and muscle. Fifteen-week-old nulliparous female Wistar rats exercised 4 weeks before as well as during gestation at a constant submaximal intensity (TR) or remained sedentary (CT). At weaning, pups from each group were fed either a standard diet (TRCD or CTCD) or a high-fat/high-sucrose diet (TRHF or CTHF) for 10 weeks. Offspring from TR dams gained less weight compared to those from CT dams. Selected fat depots were larger with the HF diet compared to control diet (CD) but significantly smaller in TRHF compared to CTHF. Surprisingly, the insulin secretion index was higher in islets from HF offspring compared to CD. TR offspring showed a higher muscle insulin sensitivity estimated by the ratio of phosphorylated protein kinase B to total protein kinase B compared with CT offspring (+48%, P < 0.05). With CD, permeabilized isolated muscle fibres from TR rats displayed a lower apparent affinity constant (Km ) for pyruvate and palmitoyl coenzyme A as substrates compared to the CT group (-46% and -58%, respectively, P < 0.05). These results suggest that maternal exercise has positive effects on young adult offspring body composition and on muscle carbohydrate and lipid metabolism depending on the nutritional status.
Published: 2017

50. Downregulation of myostatin pathway in neuromuscular diseases may explain challenges of anti-myostatin therapeutic approaches

Author: Thomas Voit, R. Joubert, Julie Dumonceaux, Laurent Servais, Caroline Bogni, Michael G. Hanna, Ana Buj-Bello, Tanya Stojkovic, Christophe Hourdé, Rozen Le Panse, Olivier Benvensite, Thierry Maisonobe, Pedro Machado, Francesco Muntoni, Léonard Féasson, Virginie Mariot, Immunologie et génétique du diabète de type 1, génétique multifactorielle en endocrinologie pédiatrique (U986), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Social and Community Medicine School, University of Bristol [Bristol], Dubowitz Neuromuscular Centre, Great Ormond Street Hospital for Children [London] (GOSH)-Institute of Child Health, Fédération de Neurophysiologie clinique, GH Pitié-Salpêtrière, Paris, Service of Clinical Trials and Databases, Institut de Myologie, GENETHON, Genethon, Centre de recherche en myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Department of Genetics, University of Cambridge, University of Cambridge [UK] (CAM), Généthon, Evry, Thérapie des maladies du muscle strié, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), UMR9196, Physiologie et Pathologie Moléculaires des Rétrovirus Endogènes et Infectieux, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Unité de Recherche PPEH, Service de Physiologie Clinique et de l'Exercice, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), The Dubowitz Neuromuscular Centre, Imperial College London, Service de Neurophysiologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Généthon, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Genetics [Cambridge], Institute of Child Health-Great Ormond Street Hospital for Children [London] (GOSH), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Université Pierre et Marie Curie - Paris 6 (UPMC), Unité de recherches Avicoles, Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Sorbonne Université (SU), Physiologie et physiopathologie des rétrovirus endogènes et infectieux (RETRO-ENDO), Institut Gustave Roussy (IGR)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Neurophysiologie Clinique [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC)
Subjects: Male, 0301 basic medicine, Follistatin, Myotubularin, Activin Receptors, Type II, General Physics and Astronomy, Myostatin, Bioinformatics, 0302 clinical medicine, lcsh:Science, Wasting, ComputingMilieux_MISCELLANEOUS, Mice, Knockout, Regulation of gene expression, Multidisciplinary, biology, Neuromuscular Diseases, Activin receptor, Middle Aged, Protein Tyrosine Phosphatases, Non-Receptor, musculoskeletal system, 3. Good health, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, medicine.symptom, Myopathies, Structural, Congenital, Adult, Science, Down-Regulation, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Downregulation and upregulation, medicine, Animals, Humans, business.industry, Skeletal muscle, General Chemistry, Disease Models, Animal, 030104 developmental biology, Gene Expression Regulation, biology.protein, lcsh:Q, business, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Muscular dystrophies are characterized by weakness and wasting of skeletal muscle tissues. Several drugs targeting the myostatin pathway have been used in clinical trials to increase muscle mass and function but most showed limited efficacy. Here we show that the expression of components of the myostatin signaling pathway is downregulated in muscle wasting or atrophying diseases, with a decrease of myostatin and activin receptor, and an increase of the myostatin antagonist, follistatin. We also provide in vivo evidence in the congenital myotubular myopathy mouse model (knock-out for the myotubularin coding gene Mtm1) that a down-regulated myostatin pathway can be reactivated by correcting the underlying gene defect. Our data may explain the poor clinical efficacy of anti-myostatin approaches in several of the clinical studies and the apparent contradictory results in mice regarding the efficacy of anti-myostatin approaches and may inform patient selection and stratification for future trials., Drugs targeting myostatin reverse muscle wasting in animal models, but have limited efficacy in patients. The authors show that the myostatin pathway is downregulated in patients, possibly explaining the poor outcome of anti-myostatin approaches, and that it can be reactivated by correcting disease-causing mutations in mice.
Published: 2017

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