1. Are IVS4 SNPs of OLR1 gene associated with coronary artery disease: Is there a linkage between IVS4 SNPs?
- Author
-
Elif Ozkok, Ozlem Kucukhuseyin, Oğuz Öztürk, Ozlem Kurnaz-Gomleksiz, Hulya Yilmaz-Aydogan, and Zehra Bugra
- Subjects
Linkage disequilibrium ,medicine.medical_specialty ,Candidate gene ,Genotype ,Medicine (miscellaneous) ,Blood lipids ,Single-nucleotide polymorphism ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Internal medicine ,Internal Medicine ,OLR1 ,Humans ,Medicine ,Genetic Predisposition to Disease ,Pharmacology (medical) ,Allele ,Genetics (clinical) ,business.industry ,Scavenger Receptors, Class E ,Endocrinology ,030228 respiratory system ,Case-Control Studies ,Reviews and References (medical) ,Restriction fragment length polymorphism ,business - Abstract
Background The OLR1 gene has been identified as a candidate gene for coronary artery disease (CAD). Six single-nucleotide polymorphisms (SNPs) of the OLR1 gene located within intron 4 (IVS4-27G>C, IVS4-73C>T, IVS4-14A>G), intron 5 (IVS5-70A>G, IVS5-27G>T) and 3'UTR (188C>T) comprise a linkage disequilibrium (LD) block, which is strongly associated with the elevated risk of CAD. Objectives We aimed to investigate the effects of the OLR1 IVS4-14A>G and -73C>T SNPs on metabolic parameters in Turkish CAD patients, and the linkage between these 2 genetic variants. Material and methods The present study was carried out in 97 CAD patients and 78 healthy individuals. The OLR1 IVS4 genotypings were performed by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. Results Serum high-density lipoprotein (HDL) cholesterol levels and body mass index (BMI) were higher in control subjects with IVS4-73CC genotype than in T allele carriers (CT+TT) (respectively, p = 0.002 and p = 0.024), while BMI values were lower in patients with CC genotype (p = 0.046). Patients with IVS4-14G allele (AG+GG) had a statistically higher low-density lipoprotein (LDL) cholesterol level (p = 0.027) than patients with -14AA genotype. Also the systolic blood pressure (SBP) levels were statistically higher in IVS4- 73C allele carriers (CT+CC) than in non-carriers (TT) (p = 0.045). A strong linkage between IVS4-14A>G and -73C>T SNPs of the OLR1 gene was detected in patients (D > 0.76). Conclusions Our results indicated that the intron 4-14A>G and -73C>T SNPs of the OLR1 gene can be inherited together. The present data also suggests that the OLR1 gene may contribute to the development of hypercholesterolemia in patients with CAD.
- Published
- 2018