1. High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
- Author
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Bo Hejdeman, Yonas Bekele, Tadepally Lakshmikanth, Francesca Chiodi, Petter Brodin, Maurizio Zazzi, and Stefan Petkov
- Subjects
CD4-Positive T-Lymphocytes ,Male ,0301 basic medicine ,Tcr signaling ,Science ,Immunology ,Programmed Cell Death 1 Receptor ,Human immunodeficiency virus (HIV) ,HIV Infections ,Protein tyrosine phosphatase ,CD8-Positive T-Lymphocytes ,medicine.disease_cause ,Microbiology ,Article ,03 medical and health sciences ,Medical research ,0302 clinical medicine ,Immune system ,medicine ,Humans ,Mass cytometry ,Multidisciplinary ,Chemistry ,Viral Load ,Flow Cytometry ,Molecular biology ,Phenotype ,In vitro ,Up-Regulation ,Blood ,030104 developmental biology ,030220 oncology & carcinogenesis ,HIV-1 ,Medicine ,Cytokines ,Leukocyte Common Antigens ,RNA, Viral ,Female ,CD8 - Abstract
Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45high T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45high cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45high T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45high T cells may contribute to their exhaustion.
- Published
- 2020