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Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients
- Source :
- Vaccine
- Publication Year :
- 2013
-
Abstract
- The relevance of CD4+T-cells, viral load and age in the immunological response to influenza infection and vaccination in HIV-1 infected individuals has previously been pointed out. Our study aimed at assessing, in the setting of 2009 A(H1N1)pdm09 influenza vaccination, whether quantification of activation-induced deaminase (AID) expression in blood B-cells may provide additional indications for predicting antibody response to vaccination in HIV-1 infected patients with similar CD4+T-cell counts and age. Forty-seven healthy controls, 37 ART-treated and 17 treatment-naïve HIV-1 infected patients were enrolled in the study. Blood was collected prior to A(H1N1)pdm09 vaccination and at 1, 3 and 6 months after vaccination. Antibody titers to A(H1N1)pdm09 vaccine were measured by hemagglutination inhibition (HI) assay while the mRNA expression levels of AID were measured by quantitative real time PCR. Upon B-cell activation in vitro, AID increase correlated to antibody response to the A(H1N1)pdm09 vaccine at 1 month after vaccination in all individuals. In addition, the maximum expression levels of AID were significantly higher in those individuals who still carried protective levels of A(H1N1)pdm09 antibodies after 6 months from vaccination. No correlation was found between CD4+T-cell counts or age at vaccination or HIV-1 viral load and levels of A(H1N1)pdm09 antibodies. Assessing AID expression before vaccination may be an additional useful tool for defining a vaccination strategy in immune-compromised individuals at risk of immunization failure.
- Subjects :
- Adult
Male
HIV Infections
Antibodies, Viral
Lymphocyte Activation
03 medical and health sciences
Influenza A Virus, H1N1 Subtype
0302 clinical medicine
Cytidine Deaminase
Influenza, Human
Activation-induced (cytidine) deaminase
Humans
Medicine
030304 developmental biology
B-Lymphocytes
0303 health sciences
Hemagglutination assay
General Veterinary
General Immunology and Microbiology
biology
business.industry
Vaccination
Public Health, Environmental and Occupational Health
Antibody titer
virus diseases
Hemagglutination Inhibition Tests
Middle Aged
Viral Load
Virology
Healthy Volunteers
CD4 Lymphocyte Count
3. Good health
Infectious Diseases
Immunization
Influenza Vaccines
Antibody Formation
Immunology
HIV-1
biology.protein
Molecular Medicine
Biomarker (medicine)
Female
Antibody
business
Viral load
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....aeb78c0f584e1f92f4db736912408007
- Full Text :
- https://doi.org/10.1016/j.vaccine.2013.03.002