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Wood burning contributes to indoor and ambient particulate matter (PM) pollution and has been associated with increased morbidity and mortality. Here, we present an integrated methodology that allows to generate, sample, and characterize wood smoke derived from different moisture contents and representative combustion conditions using pine wood as a model. Flaming, smoldering, and incomplete combustion were assessed for low-moisture pine, whereas both low-moisture pine and high-moisture pine were investigated under flaming conditions. Real-time monitoring of carbon monoxide, volatile organic compounds, and aerosol number concentration/size in wood smoke was performed. The PM was size-fractionated, sampled, and characterized for elemental/organic carbon, organic functional groups, and inorganic elements. Bioactivity of PM was assessed by measuring the sterile alpha motif (SAM) pointed domain containing ETS (E-twenty-six) transcription factor (SPDEF) gene promoter activity in human embryonic kidney 293 (HEK-293T) cells, a biomarker for mucin gene expression. Findings showed that moisture content and combustion condition significantly affected the organic and inorganic elemental composition of PM

As a renewable biological macromolecule with aromatic structure, lignin can serve as matrix substance to maintain cell wall integrity and is regarded as the natural biomass recalcitrance. Substantial differences in the cell wall lignin topochemistry between opposite (Ow) and compression wood (Cw) trachieds in Pinus bungeana Zucc. were visualized during [Emim][OAc] pretreatment at room temperature. The ionic liqiuds treatment induced a more obvious wall swelling for highly lignified Cw tracheids than that of Ow, while dynamic Raman spectra analysis indicated the higher lignin and carbohydrates removal for Ow tracheids. Raman imaging further revealed that both lignin and carbohydrates were dissolved simultaneously within the middle lamella and secondary wall of Ow and pretreatment has little effects on Cw tracheids wall. Moreover, it was demonstrated that lignin composition was the key factor to affect the composition dissolution. In particular, lignin G-units were selectively removed from cell corner middle lamella (52.3 %) and secondary wall (62.0 %) of Ow tracheids. When cotton fiber, as a reference was treated under the same conditions, lattice conversion moving from cellulose I to II occurred. The findings confirmed the important role of lignin compostion in the dissolution behavior of carbohydrate dominant tracheids wall.

Sustainable biomass resources are favored by researchers on account of their biodegradability and biocompatibility, which is a replacement for non-renewable fossil fuels. The development of low-carbon, green, and high-value bio-based adhesives are the inevitable trend of the industry development. However, the main factors limiting their application are poor water resistance and low bonding performance. Herein, the crosslinking network was constructed based on the reaction between the epoxy groups of trimethylolpropane glycidyl ether (TMPEG) and the amino groups of the synthesized aminated cellulose (AC) to form an interlocking bond. Through the synergy of covalent bond, electrostatic interaction, and hydrogen bond, the bonding strength and water resistance of the proposed adhesive can be effectively improved. Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and solid-state nuclear magnetic resonance spectroscopy (

Biomass-based adhesives are considered to be the preferred alternative to formaldehyde-type wood adhesives due to their wide range of sources, low cost, and sustainability. Herein, an environmentally friendly Schiff base cross-linked compact three-dimensional network structure bio-adhesive (DAC-PEI-U) derived from polyethyleneimine (PEI), urea, and cellulose was successfully prepared, verifying by detailed FTIR, NMR, and XPS analysis. Schiff base bridging between aldehyde groups in dialdehyde cellulose (DAC) and amino groups in polyurea (PEIU) not only constructed crosslinking networks but also endowed adhesives with good adhesion property. The dry bond strength of DAC-PEI-U adhesive reached 2.71 MPa, and the wet shear strength was 1.51 MPa (hot water) and 1.34 MPa (boiling water), respectively. It not only improves the water resistance and bonding process, but also displays simple synthesis and low cost. The improved performance of DAC-PEI-U adhesive is attributed to the generation of hyperbranched cross-linking structure in the adhesive system, which results in increased cross-linking density and promotes the formation of dense cross-sections in the curing adhesive. This work paves a solid way for developing cellulose-based wood adhesives with wet bonding properties, thus holding great potential as an alternative to formaldehyde-type adhesives in wood-based panel and indoor panel bonding industries.

The current study investigates for the first time the physico-chemical performances of lignins from cactus waste seeds (CWS) and spent coffee (SC) in comparison to previously isolated lignins from sugar byproducts (bagasse (SCB) and beet pulp (SBP)). In this work, lignin-phenol formaldehyde (LPF) resins were formulated using various lignin loadings (5-30 wt%), characterized and applied in the manufacturing of plywood panels. Several characterization techniques were applied to identify the chemical and morphological properties, thermal stability, and phenolic content of the extracted lignins, as well as the bonding strength and wood failure of the formulated resins. Results showed that the CWS and SC could be considered as an important source for lignin recovery with a considerable yield of 15.46 % and 27.08 % and an important hydroxyl phenolic content of 1.26 mmol/g and 1.36 mmol/g for CWS and SC, respectively. Interestingly, 20 wt% of extracted lignins in PF adhesives were the optimal formulation showing an improved modulus of elasticity (MOE) of about 3505, 3536 and 3515 N/mm

The cellulose nanocrystals (CNCs) were produced from spruce wood using less hazardous and toxic reagents with understanding of influence of process parameters on CNCs properties. This study employed acetosolv pulping followed by alkaline-peroxide bleaching, eliminating highly reactive chemicals such as Na-chlorites and Na-sulfite for cellulose pulp extraction from spruce wood. Cellulose pulp yield of 41.5 ± 0.7 wt% of dry wood was obtained from pulping followed by bleaching treatment. Cellulose pulp was hydrolyzed with 59.0-65.0 wt% sulfuric acid followed by ultrasonic treatment to produce CNCs. CNCs yield of 8.0 ± 3.2 wt% of dry wood was obtained at 65 wt% acid concentration and yield of 25.1 ± 0.7 wt% at 62 wt% acid concentration. The optimization of acid hydrolysis and ultrasonic treatment resulted in CNCs with high aspect ratios (length/width) up to 48.1. It was demonstrated that higher acid concentration requires lower intensity of ultrasonic treatment for CNCs dispersion, and that higher intensity could enhance aspect ratio without impacting the crystallinity index. However, ultrasonic treatment for longer than 5 min led to destruction of the whisker morphology of CNCs. The extracted CNCs possess high crystallinity index of 80.8 ± 1.7 %, low residual hemicellulose (2.0 %) and lignin (0.7 %), and high-char content of 26.7 wt% from thermal degradation.

Wood and wood-based materials are used by being processed in various machines in furniture, joinery and construction industries. Various cutting forces arise based on mechanics of machining. It is stated that the cutting power and power consumption factors depend on the cutting forces. The cutting forces are especially used as the main output in the analysis of the cutting process and physico-mechanical cutting models are very important in understanding the cutting factors. Vibration, noise, temperature and machining defects as well as cutting power, surface and chip quality are usually not considered. Determination of optimal machining conditions with efficient and economical works are based on material, tool geometry and mechanics of machining in terms of obtaining smooth surfaces with the lowest power consumption in machining. In this research, the factors affecting the mechanics of machining in the processing of wood and wood-based materials are discussed. Cutting forces, cutting power and their measurement methods with tool and machining geometry were investigated.g geometry were investigated., Odun ve odun kökenli malzemeler mobilya, doğrama ve yapı endüstrilerinde çeşitli makinelerde işlenerek kullanılmaktadır. İşleme mekaniklerine dayalı çeşitli kesme kuvvetleri ortaya çıkmaktadır. Kesme gücü ve güç tüketimi faktörlerinin kesme kuvvetlerine bağlı olduğu belirtilmektedir. Kesiş sürecinin analizinde özellikle kesme kuvvetleri ana çıktı olarak kullanılmakta olup, kesişte etkili faktörlerin daha iyi anlaşılmasında fiziko-mekanik kesiş modelleri oldukça önemlidir. Odun ve odun kökenli malzemelerin fiziksel ve teknolojik özellikleri, makinede işleme koşulları ve kesici aletlerin mekanik durumu işleme mekaniklerini etkileyen faktörler olarak belirtilmektedir. Titreşim, ses, sıcaklık ve işleme kusurları yanında kesme gücü ile yüzey ve yonga kalitesi genellikle göz önünde bulundurulmamaktadır. İşlemede en düşük güç tüketimi ile düzgün yüzeyler elde edilebilmesi bakımından verimli ve ekonomik çalışmalar ile uygun işleme koşullarının belirlenmesi, malzeme ve kesici geometrisi ile işleme mekaniklerine dayandırılmaktadır. Bu araştırmada, odun ve odun kökenli malzemelerin işlenmesinde işleme mekaniklerini etkileyen faktörler tartışılmıştır. Kesme kuvvetleri, kesme gücü ve bunların ölçüm yöntemleri ile kesici ve işleme geometrisi incelenmiştir.

To promote the development of the green and low-carbon wood industry and explore the current status and trend of China's used-furniture recycling, this paper summarizes the research status of used-furniture recycling and interprets governmental strategies, such as the development of used-furniture recycling system, the way of handling used-furniture by manufacturers, and the attitudes of customers towards used-furniture, through extensive literature reviews and market research. From such three respects as government, furniture manufacturers, and consumers, the problems of national used-furniture recycling are analyzed, for example, imperfect system, high cost, and weak awareness. Therefore, to solve the above issues, it is necessary to construct national used-furniture recycling system and methods. The government should pay attention to the construction of recycling network, the supervision and support of entrepreneurial responsibilities, and the formulation of recycling standards. For furniture manufacturers, the improvement of the durability and recyclability of furniture and the development of new models and new technologies for furniture recycling and transportation are their primary assignment. And for consumers, the improvements of their concepts of consuming and the environmental protection are in focus. In sum, it aims to provide both suggestions for the amelioration of the measures and methods, and support for the understanding of the change, of used-furniture recycling in China.

A Bronze Age palstave found at Emmen. In 2019, a Bronze Age palstave was found in a field west of Emmen by a father-and-son team of metal detectorists. In agreement with the landowner, the find was reported to PAN. During the process of conservation, the opportunity presented itself to study the alloy using pXRF analysis. This suggested that the copper ores used in the bronze alloy ultimately derived from Swiss or Slovakian mines, but that the metal was cast locally into a form well-known in the sandy regions of the Low Countries. A review of the find in its topographical and cultural context indicated that it – like various other items in the Bronze Age – had been deposited in (the margin of) wetland parts of the landscape, not far from a contemporaneous settlement.

The high density and poor thermal insulation of traditional wood-plastic composites limited the application in the field of building materials. In this paper, wood fiber (WF) and PLA were used as raw materials and azodicarbonamide was used as the foaming agent. Lightweight WF/PLA composites were prepared by the hot-pressing foaming method, aiming to obtain renewable, low-density material with high strength-to-weight ratio and thermal insulation performance. The results showed that after adding 20 % WF into PLA, the cell morphology was excellent and the cell size was uniform. The magnification reached the minimum value of 0.36 g/cm

Indoor concentrations of black carbon (BC) were measured when wood was burned for traditional cultural activities in a study in a Cree community located in subarctic Canada. The study also included an intervention using a propane-fuelled heater to mitigate in situ BC. Mass concentrations of BC were measured in a game-smoking tent for 39 days and in hunting cabins on the west coast of James Bay, Canada, for 8 days. Five-minute averaged BC mass concentration (N = 12,319) data were recorded and assessed using optimised noise-reduction averaging. Mean BC mass concentrations were lower in hunting cabins (mean = 8.25 micrograms per cubic metre (µg m−3)) and higher in the game-smoking tent (mean = 15.46 µg m−3). However, excessive BC peaks were recorded in the game-smoking tent (maximum = 3076.71 µg m−3) when the fire was stoked or loaded. The intervention with the propane heater in a hunting cabin yielded a 90% reduction in measured BC mass concentrations. We do not presume that exposure to BC is of concern in hunting cabins with appropriate wood-burning appliances that are well-sealed and vent outside. In game-smoking tents, we advise that persons take intermittent breaks outside of the tent for fresh air.

Chef-d’œuvre du département des Sculptures du musée du Louvre, le Christ dit Courajod (RF 1082) a été étudié puis restauré dans les ateliers du C2RMF entre 2007 et 2011. La polychromie de cette œuvre présentait un état de conservation médiocre, résultant essentiellement d’une tentative de dégagement non maîtrisé en 1956. Cette intervention a créé un état de surface confus induisant une perte de visibilité des qualités plastiques de la sculpture. L’élimination de l’ensemble des repeints a permis de découvrir une polychromie originale romane d’une facture remarquable et d’une grande finesse, aux coloris subtils jouant sur un contraste entre des carnations pâles, des cheveux et une barbe d’un noir soutenu et un périzonium d’un bleu intense rehaussé d’or. A masterpiece of the Louvre’s Sculpture Department, the figure of Christ from a Descent from the Cross, known as the Courajod Christ (RF 1082), was examined then restored in the C2RMF workshops between 2007 and 2011. The polychromy of this work was in a mediocre condition, basically as a result of a misfired attempt to remove overpaints in 1956. This restoration left the work with a muddled surface concealing some of the sculpture’s artistic qualities. The removal of all the repaints revealed the great finesse and remarkable handling of the original Romanesque polychromy. Its subtle use of colour plays on a contrast between the pale flesh tints, the dark hair and beard and the deep blue perizonium enhanced with gold.

Exploring the reusability of wood-based panels is imperative in the wood industry for sustainable development and carbon balance. Non-reusable adhesives make wood-based panel recycling difficult. In this study, inspired by the adhesion and de-adhesion behavior of snail slime, we built dynamic covalent/hydrogen hybrid networks into adhesive system for achieving both high bonding performance and reusability. Specifically, the softwood lignin was purified and pretreated by ultrasonication to form a catechol structure (UAL) and then combined with soybean protein to develop a 100 % bio-based wood adhesive. The catechol structure of UAL formed dynamic covalent bonds (CN) with the amino groups of the protein to improve the water resistance and formed multiple hydrogen bonds as a sacrificial network to improve the toughness of the adhesive. Thus, the wet shear strength of plywood bonded by the resultant adhesive improved by 101.4 % to 1.37 MPa. The adhesive also exhibited flame retardancy (LOI = 37.7 %), mildew resistance (60 h), and antibacterial performance (inhibition zone = 8 mm). Notably, owing to the rearrangement of dynamic covalent/hydrogen hybrid networks and the thermoplastic property of UAL, the resultant adhesive was reusable (3 cycles) and degradable (2 months), which provides a potential method for the reuse of wood-based panels.

Irregular hemorrhagic traumas always threaten the health of patients due to uncontrollable bleeding and wound infections. The traditional hemostatic materials show dissatisfactory hemostatic efficiency and antibacterial activity in solving these potential bleeding dangers. Herein, we proposed a kind of composites based on flexible wood membrane (FWM) loaded with chitosan/alginate derivative for accelerating rapid hemostasis and preventing infection. FWM was removed part of hemicellulose and lignin by using NaOH/Na

Hemicellulose is an important component of the plant cell wall which vary in structure and composition between plant species. The research of hemicellulose structures is primarily focused on fast-growing plants during xylogenesis, with slow-growing and rare trees receiving the least attention. Here, hemicellulose structure of the rare species Castanopsis hystrix during xylogenesis was analyzed. Acetyl methyl glucuronide xylan was the most common type of hemicellulose in C. hystrix, with a unique tetrasaccharide structure at the reducing end. Hemicellulose type, structure, molecular weight, thermal stability, biosynthesis and acetyl substitution content and pattern remained stable during the xylogenesis in C. hystrix, which could be attributed to its slow growth. The stable polymer type, low side chain modification and high acetyl substitution of hemicellulose throughout the stems are among the reasons for the hardness and corrosion resistance properties of C. hystrix wood. Genetic modification can be used to improve these properties.

This study was undertaken to determine the effect of wood dust on the respiratory system and oxidative stress in furniture workers and to determine whether any associations exist between respiratory parameters and oxidative stress.This cross-sectional study was performed on 45 furniture workers and 45 office workers as a reference group in Iran. The NIOSH method 0600 was used to determine the concentration of particulates. The prevalence of respiratory symptoms was estimated via the European Community Respiratory Health Survey (ECRHS) questionnaire. Oxidative stress biomarkers and respiratory parameters were also measured.The mean concentrations of respirable and non-respirable dust were found to be 1.51 mg/mThe present study results indicated that exposure to wood dust significantly increased respiratory disorders and confirmed the association between lung function parameters and oxidative stress.

As a soybean oil extractive byproduct, high temperature defatted soy meal (HSM) presents great potential as a raw material for vegetable protein adhesives to replace aldehyde-based adhesives in the wood-based panel production. However, the application has been hindered by its poor cold-pressing adhesive performance. Herein, a novel HSM-based adhesive with excellent cold-pressing adhesion performance was developed based on mussel-inspired cation-π interactions. Highly reactive polyamidoamine-epichlorohydrin (PAE) and folic acid (FA) were added into an HSM-based adhesive to construct a dual-network system stabilized by strong cation-π interactions. The coacervate formed by PAE and FA served as an "internal adhesive" to bond HSM particles together, yielding high initial viscosity but easy sizing. As expected, the prepared adhesive exhibited an excellent cold-pressing bonding strength of 423 kPa, showing a 295% improvement compared to the soy protein (SP) adhesive. To improve the hot-pressing bonding strength of the adhesives, inorganic calcium carbonate (CaCO

To reveal the existence of p-hydroxyphenyl (H) units in compressed wood lignin, four different milled wood lignins were extracted using Pinus massoniana Lamb compressed wood, Pinus massoniana Lamb normal wood, and sugarcane bagasse as raw materials. Then, three dehydrogenation polymers (DHPs) were synthesized using coniferyl/p-coumaryl alcohol as raw materials to reveal the interunit linkages of H units. The lignin and DHP samples were systematically characterized by

Human health and environmental protection demand wood-based panel industry for innovative soy-based adhesives with high production efficiency, straightforward synthesis processes, non-toxicity, and high bonding performance. A simple and efficient microwave pretreatment process and low addition of bio-derived crosslinking agent was used in this study to prepare a non-toxic and high-bonding performance soybean protein-based adhesive. After 4 min of microwave pretreatment time, the complex quaternary structure of soybean protein molecule unfolds, the soybean protein disperses evenly and stably, and active groups of soybean protein molecules are exposed. After adding 3.85% crosslinking agent, the moisture absorption rate of the soybean protein-based adhesive decreases by 41.77%, the residual rate increases by 3.68%, and the wet shear strength of the resultant plywood increases to 1.12 MPa, which satisfies requirement of interior use plywood. Compared with previously reported soy-based adhesives, this adhesive is dependent on fewer chemical reagents, but has good bonding performance. The 204.41% of relative cell viability indicates the resultant adhesive was non-toxic. The proposed high-efficiency, high-performance, non-toxic biomass adhesive has great prospects for the industrial application.

Lung cancer is the leading cause of death among cancer patients. The majority of lung cancer is the Non-Small Lung Carcinoma (NSLC). This study evaluated the potency of brazilin isolated from Caesalpinia sappan wood to induce apoptosis on non-small lung carcinoma cell line, A549, by examining the expression of p53, caspase-9, and caspase-3.Brazilin was isolated from Caesalpinia sappan wood following a guided assay and it was determined by using Brazilin®SIGMA as standard. The activity of brazilin on the growth of A549 cell line was analysed by MTT assay and the apoptosis was evaluated by flowcytometer following Annexin V (FITC) and PI staining. The expression of p53, caspase-9, and caspase-3 was examined by immunocytochemistry.The IC50 of brazilin on A549 cell line was 43µg/mL. Cell treatment with 20 µg/mL and 40 µg/mL of brazilin significantly increased early apoptosis (p0.001). Cell treatment with 40 µg/mL of Brazilin significantly increased late apoptosis (p0.001). Brazilin significantly increased the expression of p53, Caspase-9, and caspase-3 (p0.001).This study showed evidence of the activity of brazilin to induce intrinsic apoptosis on a NSLC cell line A549.

Air is one of the most essential elements that living things need to survive. Air pollution has become even more important due to reasons such as population growth and the difficulties brought by global warming and war. The factors that make up air pollution are also one of the most important factors of environmental pollution and cause serious pollution problems. While some metals in the air positively affect plant growth as nutrient elements in agricultural activities, they cause deterioration of plant development due to the accumulation of high concentrations in plant organs. This study, as an indicator of the time-dependent variation of Mg and K concentrations with atmospheric accumulation, reveals the variation and year-related amounts ofPinus pinasterspecies in Düzce province.&nbsp

Natural resistance of some wood species used consistently for construction in Nigerian to termite was examined in this study. Wood samples were collected from a Sawmill in Akure and cut to stakes of 35 × 35 × 450 mm which were to be buried half way in the soil, their density was determined and classified across three density classes; high, medium and low. They were exposed to subterranean termites attack for 12 weeks (3 months) in a timber graveyard. Weekly visual estimation of the stakes was done in accordance with ASTM D 3345/1980 rating scale and gravimetric weight loss assessment was carried out after 12 weeks of exposure. Results showed that there was significant difference (p

A bud bank is a pool of dormant meristems that enable plants to resprout after injury. While the bud bank on stem organs is established prior to injury as the stem grows, the bud bank on roots is considered at least partly formed as a response to disturbance events. To date, only woody species have been examined, and the establishment of reparative buds after injury without connection to the root vascular system has been confirmed; for herbs, no data are available. We tested whether root buds are formed spontaneously or induced after plant damage by studying root anatomy following plant injury in two congeneric perennial herbs. In a pot experiment with young plants of Inula britannica (root sprouter) and I. salicina (non-root sprouter), whole aboveground biomass was removed. Roots were sampled five times at 1-week intervals after disturbance events to evaluate bud occurrence and size, root and vessel diameters, sclerenchyma areas and carbohydrate storage. Compared to non-root-sprouting I. salicina, root-sprouting I. britannica presented more secondary thickening that was connected to adventitious bud formation and improved the root storage and transport capacity necessary for resprouting. Plant injury, in contrast to expectations, did not cause increased bud formation in I. britannica, and all buds were connected to the root vascular system. No root buds were observed in I. salicina. Our study implies that plants using bud banks on roots might depend on preformed buds. Comparative studies examining more species are needed to assess the generality of our findings.

Fungi play a significant role in wood fiber degradation since they possess enzymatic tools for the degradation of recalcitrant plant polymers. The study aims to demonstrate the interactive fungal traits when they grow together and its development with total dead wood fiber degradation speed. A lab experiment was designed to describe decomposition rates and fungal properties using nonlinear fitting model and logistic equation from preliminary data sets. The degradation speed of five (A, B, C, D, and E) different types of fungi with different growth rates were calculated at various relative humidity’s (35, 50, 65, 80, and 95 g.kg−). Results showed that the mycelium length of fungus A, has faster ideal growth rate than that of fungus B, with ecological niche width A

Eremanthus erythropappus hosts globoid stem galls induced by Neolasioptera sp. (Diptera: Cecidomyiidae) close to the stem apex, which do not compromise the shoot apical meristem (SAM). We hypothesize that maintenance of the SAM, as well as the increasing number of leaves per branch and of galled stem lengths and diameters, are a consequence of alterations in vascular cells and, consequently, in the priority for water flow from non-galled stems to the stem galls. Our study focuses on the globoid stem galls to evaluate if gall induction and development promote changes in structure and function of secondary xylem cells. Anatomical, cytological, histometric and physiological methods were used to analyse non-galled stem branches (NGS), mature globoid stem galls and stem portions below and above the galls. These analyses revealed that vessel elements are larger in stem galls and in stem portions above the galls. Under Neolasioptera sp. induction activity, the vascular cambium of E. erythropappus produces less numerous but larger vessel elements and overproduces parenchyma cells. Contrary to the vascular constriction hypothesis proposed for bacterial galls, the vascular traits of the Neolasioptera sp. stem galls on E. erythropappus result in priority for water flow to galls and the non-galled portions above the galls, allowing the maintenance of galled stem growth and development.

Preservative treatments increase the durability of wood, and one of the alternative treatments involves the use of chromated copper arsenate (CCA). Due to the toxicity of CCA, the disposal of CCA-treated wood residues is problematic, and burning is considered to provide a solution. The ecotoxicological potential of ash can be high when these components are toxic and mutagenic. The aim of this study was to evaluate the toxicity and genotoxicity of bottom ash leachates originating from CCA-treated wood burning. Physical-chemical analysis of the leachates revealed that in treated wood ashes leachate (CCA-TWBAL), the contents of arsenic and chromium were 59.45 mg.L

Slurry fuels based on wood and coal processing and petroleum refinery waste are an environmentally friendly and economically feasible alternative to the conventional solid fuel-coal. As part of this experimental research, we compared a set of fuels (coal and coal-water slurries with and without petrochemicals) by normalizing and calculating the specific concentrations of pollutants from their combustion. The pollutant concentrations were normalized with respect to the mass of burnt fuel, the thermal energy released by combustion, specific mass emissions per unit time, specific maximum mass emissions, and specific mass emissions per 1 kg of fuel equivalent or 1 MJ of thermal energy. The key objective of this research was to develop a method for comparing composite fuels in terms of their relative environmental friendliness. As part of the research, coal combustion was notable for the peak emissions of gaseous pollutants irrespective of the fuel mass and combustion chamber temperature. When slurries were burnt, CO

Being one of the most common wood in furniture and decoration, Fraxinus mandshurica Rupr. (F. mandshurica) is a frequently used material for wood products. However, the resulting odor problem has not yet been solved so far. Thus, this study focused on exploring odor-active compounds and investigating the effect of environmental conditions on emission and odor attributes. F. mandshurica was studied via TD-GC-MS/O combined with microchamber. Totally, 15 kinds of key odor-active compounds were detected from F. mandshurica. The main odorous components were found to be aromatics, aldehydes and ketones, esters, alcohols and acids. Six kinds of attributes played a key role in the overall odor character of F. mandshurica. Fragrant was the predominant odor impressions, followed by vinegar and antiseptic solution. The proportion of the odor-active compounds concentration to total concentration decreased with an increase in the temperature and relative humidity, while the ratio of air exchange rate to the loading factor had little effect on this. The total concentration of F. mandshurica increased as the temperature and relative humidity increased, but these decreased as the ratio of air exchange rate to the loading factor increased, while the total odor intensity reduced with the rise in the ratio of air exchange rate to the loading factor. Time was found to be the most effective factor for eliminating emissions. Considering the environmental conditions in this study, 40 °C, 60% relative humidity and a 1.0 m

Here, water-suspended smoke aerosol preparation was synthesized from biomass-based fuel, i.e., a widespread product for residential heating, wood sawdust (WP) (pine, poplar, and birch mixture), and its properties were compared in parallel experiments with the smoke preparation from plastics (PP). Molecular groups in the PM preparations were analyzed using Raman and Fourier-transform infrared spectroscopy. WP was assessed in neurotoxicity studies using rat cortex nerve terminals (synaptosomes). Generation of spontaneous and H2O2-evoked reactive oxygen species (ROS) detected using fluorescent dye 2′,7′-dichlorofluorescein in nerve terminals was decreased by WP. In comparison with PP, WP demonstrated more pronounced reduction of spontaneous and H2O2-evoked ROS production. WP completely inhibited glutamate receptor agonist kainate-induced ROS production, thereby affecting the glutamate receptor-mediated signaling pathways. WP decreased the synaptosomal membrane potential in fluorimetric experiments and the synaptosomal transporter-mediated uptake of excitatory and inhibitory neurotransmitters, L-[14C]glutamate and [3H] γ-aminobutyric acid (GABA), respectively. PP decreased the ambient synaptosomal level of [3H]GABA, whereas it did not change that of L-[14C]glutamate. Principal difference between WP and PP was found in their ability to influence the ambient synaptosomal level of [3H]GABA (an increase and decrease, respectively), thereby showing riskiness in mitigation of synaptic inhibition by PP and triggering development of neuropathology.

Les na prostem je izpostavljen delovanju raznolikih dejavnikov razkroja, še posebno glivam. Nekatere lesne vrste so bolj odporne na glivni razkroj kot druge. V Kostanjevici na Krki že 60 let deluje forma viva, kjer so razstavljeni kipi, izdelani pretežno iz hrastovega lesa. Tanaka Eisaku, japonski kipar, je izdelal najstarejši kip z naslovom Japonski festival na prvi kostanjeviški Formi vivi leta 1961. Po 60 letih zunanje izpostavitve smo kip vizualno pregledali in ocenili njegovo stanje z uporabo rezistografa in vlagomera. Iz kipa smo izolirali nekaj vzorcev, jih preiskali s FTIR in XRF spektroskopijo, vzorcem določili gostoto s piknometrom GeoPyc in jih analizirali z lasersko in digitalno mikroskopijo. Na kipu je opaziti znake glivnega razkroja, izletne odprtine insektov in poškodbe zaradi delovanja ptic. Ocena razkrojenosti z rezistografom je pokazala, da je les razkrojen predvsem na čelih in v sredici. Poleg tega je na nekaj mestih močno razkrojena tudi beljava. Les je bil v zadnjih letih obdelan s površinskim premazom, po drugi strani v lesu ni bilo mogoče zaznati ostankov biocidov. V primeru, da želimo kip ohraniti zanamcem, ga je treba ustrezno konzervirati. Wood in outdoor applications is exposed to various decay factors, of which fungi are the most important. Some wood species are more resistant to fungal decay than others. Forma Viva in Kostanjevica has been exhibiting sculptures made mainly of oak wood for 60 years. The oldest sculpture is the work of Tanaka Eisaku entitled “Japanese Festival”. After 60 years, the condition of the sculpture was examined with a resistograph and a moisture metre. Several samples were isolated from the sculpture and examined with FTIR and XRF spectroscopy. The density of the samples was determined with GeoPyc and analysed in detail with laser and digital microscopy. Signs of fungal attack, as well as insect and bird damage can also be seen on the sculpture. The resistograph analysis showed that the wood is mainly decayed in the axial planes and in the core. In addition, the sapwood is severely decayed in some areas. The wood has been surface-coated in recent years however, there is no biocide residue in the wood. If the sculpture is to be preserved for posterity, it must be properly conserved.

Pure deep eutectic solvents (DESs) system of choline chloride (ChCl)/Lactic acid (Lac) were demonstrated to be an effective strategy for extraction of lignin. In this study, two kinds of different promising solid acid (SA) with DESs were designed to promote the pretreatment of lignocellulose. The SA of phosphotungstic acid (H3O40PW12) and iron bromide (FeBr3) were introduced into DESs to extract poplar wood lignin and evaluate the antioxidant activity. It was found that 82.2% and 80.9% of lignin were obtained from poplar wood under H3O40PW12-ChCl/Lac acid and FeBr3-choline ChCl/Lac system with 4 h and 8 h, respectively. The lignin fractions with a high purity (>89%), low molecular weight (Mw 1800–2000 g/mol). Besides, the antioxidant activities of lignin fractions were better than butyl hydroxyanisole (BHA). Therefore, DES lignin has prominent antioxidant activity and could developed a potential natural cosmetics and food packaging.

Due to the dependence on petroleum to supply energy and chemical needs many countries have experienced problems because of declining reserves, increased demands, and political problems in oil exporting nations, which have also created problems for oil-importing countries. Demand on renewable resources to meets the needs for energy and carbon based chemicals in the wake of eventual depletion of fossil oil and natural gas reserves. Furfural, lignin, vanillin can be synthesized by extraction process from which vanillin and furfural are costlier products, which makes the process economically viable. Material balance was done to understand the material composition of wood. Comparison and costing of various process of are also evaluated which resulting into inexpensive than petroleum. A long-term solution would be to use renewable resources to produce many of the hydrocarbons. So, this work aims at finding feasible ways extracting the valuable chemicals such as furfural, lignin, vanillin, etc. from wood.

Oxalate oxidase (EC 1.2.3.4) is an oxalate-decomposing enzyme predominantly found in plants but also described in basidiomycete fungi. In this study, we investigated 23 fungi to determine their capability of oxalic acid degradation. After analyzing their secretomes for the products of the oxalic acid-degrading enzyme activity, three groups were distinguished among the fungi studied. The first group comprised nine fungi classified as oxalate oxidase producers, as their secretome pattern revealed an increase in the hydrogen peroxide concentration, no formic acid, and a reduction in the oxalic acid content. The second group of fungi comprised eight fungi described as oxalate decarboxylase producers characterized by an increase in the formic acid level associated with a decrease in the oxalate content in their secretomes. In the secretomes of the third group of six fungi, no increase in formic acid or hydrogen peroxide contents was observed but a decline in the oxalate level was found. The intracellular activity of OXO in the mycelia of Schizophyllum commune, Trametes hirsuta, Gloeophyllum trabeum, Abortiporus biennis, Cerrena unicolor, Ceriosporopsis mediosetigera, Trametes sanguinea, Ceriporiopsis subvermispora, and Laetiporus sulphureus was confirmed by a spectrophotometric assay.

Extensive research efforts have been devoted to describing yeast alcohol oxidase (AO) and its promoter region, which is vastly applied in studies of heterologous gene expression. However, little is known about basidiomycetous AO and its physiological role in wood degradation. This review describes several alcohol oxidases from both white and brown rot fungi, highlighting their physicochemical and kinetic properties. Moreover, the review presents a detailed analysis of available AO-encoding gene promoter regions in basidiomycetous fungi with a discussion of the manipulations of culture conditions in relation to the modification of alcohol oxidase gene expression and changes in enzyme production. The analysis of reactions catalyzed by lignin-modifying enzymes (LME) and certain lignin auxiliary enzymes (LDA) elucidated the possible involvement of alcohol oxidase in the degradation of derivatives of this polymer. Combined data on lignin degradation pathways suggest that basidiomycetous AO is important in secondary reactions during lignin decomposition by wood degrading fungi. With numerous alcoholic substrates, the enzyme is probably engaged in a variety of catalytic reactions leading to the detoxification of compounds produced in lignin degradation processes and their utilization as a carbon source by fungal mycelium.

The effects of long-term feeding of wood kraft pulp (KP) silage containing sweet-potato shochu distillery by-product (SDP) on feed intake, feed digestibility, rumen fermentation, and growth performance of Japanese Black steers were investigated during the early fattening period. Ten Japanese Black steers (9.8 ± 0.6 months of age) were used in this study. Five steers (KP group) were fed KP silage as a replacement for 10% timothy hay (dry matter bases), in contrast to the other five (control group). KP silage consisted of 92.9% KP and 7.1% SDP (dry matter bases). The experiment was conducted for 18 weeks. No significant differences were observed in terms of feed intake, feed digestibility, or daily body weight gain between the groups. In addition, diurnal changes in the rumen pH and ruminal lipopolysaccharide activity did not differ between the groups. However, the plasma concentration of aspartate transaminase in the KP group was slightly lower (P = 0.078) than that in the control group. Thus, our study suggested that feeding KP silage does not reduce feed intake or affect the rumen fermentation or growth performance of Japanese Black fattening steer.

Particulate matter from wood burning emissions (Cwood) was quantified at five locations in the United Kingdom (UK), comprising three rural and two urban sites between 2009 and 2021. The aethalometer method was used. Mean winter Cwood concentrations ranged from 0.26 μg m-3 (in rural Scotland) to 1.30 μg m-3 (London), which represented on average 4% (in rural environments) and 5% (urban) of PM10 concentrations; and 8% of PM2.5. Concentrations were greatest in the evenings in winter months, with larger evening concentrations in the weekends at the urban sites. Random-forest (RF) machine learning regression models were used to reconstruct Cwood concentrations using both meteorological and temporal explanatory variables at each site. The partial dependency plots indicated that temperature and wind speed were the meteorological variables explaining the greatest variability in Cwood, with larger concentrations during cold and calm conditions. Peaks of Cwood concentrations took place during and after events that are celebrated with bonfires. These were Guy Fawkes events in the urban areas and on New Year's Day at the rural sites; the later probably related to long-range transport. Time series were built using the RF. Having removed weather influences, long-term trends of Cwood were estimated using the Theil Sen method. Trends for 2015-2021 were downward at three of the locations (London, Glasgow and rural Scotland), with rates ranging from -5.5% year-1 to -2.5% year-1. The replacement of old fireplaces with lower emission wood stoves might explain the decrease in Cwood especially at the urban sites The two rural sites in England observed positive trends for the same period but this was not statistically significant.

Developing strategies that counter the ongoing homogenization trends of home-garden agroforestry systems is required to maintain diversity and sustainability. This study aimed to map and characterize traditional enset-based home-garden agroforestry for managing sustainability in the Gurage socioecological landscape in Ethiopia. We generated plots and land use land cover (LULC) spatial data from orthophotomosaic and collected household survey data of the field. Five home-garden types were identified explicitly through integrating the home-garden composition, functional structure, and agroecological zones. Most home-garden types had similar horizontal functional structures in which perennial crops were planted close to homesteads, annual crops grew in outer fields, and woodlots were located at the end of the parcel. Diverse woody species, crop varieties, and plot sizes were identified in individual household parcels, and these varied across the home-garden types. Enset-based home-garden agroforestry production has been declining in the Ethiopian landscape because of socioeconomic changes and a lack of technological inputs. These challenges may compromise the community's food security with loss of the product diversity provided by the home-garden system. Thus, technological adoptions and scaling up of agroforestry practices according to the home-garden types are necessary for the continue provision of multiple contributions. This study demonstrated site-specific spatial characterization of the agroforestry systems by considering a holistic approach to reduce the local challenges and support the development of sustainable landscape management in an altering socioecological landscape.

The present study seeks to investigate the use of husbandry waste and sawdust in the construction of degradable pots as a suitable alternative to plastic pots. Six mixture ratios of cattle manure and sawdust (85:15, 80:20, 75:25, 70:30, 65:35, and 60:40) were used along with three types of natural binders (sheep's wool, cornstarch, and sheep's wool:cornstarch) in phase I of the project. Phase I was replicated in triplicate to identify the best composition for each binding agent. International standards dictate that evaluations of biological pots include investigations into thickness swelling, internal bonding, and water absorption. Mean comparison of the resultant factorial data using the Tukey and TOPSIS methods indicated that production of bio-pots with a mixture of 80% manure and 20% sawdust may provide the best results for all three pot types. Phase II of the project involved using field experiments and cultivation of tomato plants in direct comparison to a commercial sample pot. The optimal pots for each binding agent in phase I were used in the evaluation. Field tests showed pots produced with 80% manure and 20% sawdust using cornstarch for binding adhesion performed best in terms of degradability and physical and mechanical properties.

Wood from invasive tree species Acacia dealbata and Ailanthus altissima was used to produce high value-added nanocellulose. Firstly, bleached pulps were produced from the wood of these tree species after kraft cooking. Afterwards, the resultant pulps were pre-treated by TEMPO-mediated oxidation (Acacia dealbata) or enzymatic hydrolysis (Ailanthus altissima) followed by high-pressure homogenization. Hydrogels were obtained and characterized for their main physical and chemical properties, including rheology measurements. After freeze-drying, the surface properties of the materials were evaluated by inverse gas chromatography. Results showed that nano/micro fibrils could be obtained from the wood of these invasive species. Rheometry studies showed that Acacia-TEMPO cellulose nanofibrils form strong gels with high yield stress point and viscosities (reaching ca. 100,000 Pa·s). Additionally, the surfaces of the obtained nanocelluloses showed a dispersive component of the surface energy near 40 mJ/m2 and a prevalence of the Lewis acidic character over the basic one, as typical for cellulose-based materials. Finally, films with good mechanical and optical properties could be obtained from the cellulose hydrogels. Acacia-TEMPO film (produced by filtration/hot pressing) showed a tensile strength of 79 MPa, Young's modulus of 7.9 GPa, and a transparency of 88%. The water vapor barrier, however, was modest (permeability of 4.9 × 10−6 g/(Pa·day·m)).

Lignocellulose including cellulose, lignin, and hemicellulose could be extracted from wood, and has been used to prepare carbon electrode. However, complicated extraction greatly increases preparation cost. To achieve maximum utilization of lignocellulose and avoid complicated extraction, wood with porous structure and good mechanical strength is used as carbon precursor. Additionally, chemical activation is commonly used to create micropores to provide high capacitance, but it brings in natural structure destruction, and generation of wastewater during pickling. Moreover, to achieve desirable energy density, multi-step strategy with long duration is required for loading active materials on carbonized lignocellulose (CL). Herein, a one-step method is developed to prepare a free-standing hybrid CL electrode (CLE) by using Lewis acid in three aspects: (1) as structure protection agent, (2) as activating agent, (3) as active materials donor, which bypasses pickling and further avoids the generation of wastewater. Additionally, natural vessels in wood can not only provide large space for active materials loading, but also act as rapid ions diffusion way, simultaneously confining active materials detachment. Benefiting from the synergistic effect of porous structure and Lewis acid, this work not only makes full utilization of lignocellulose, but also makes CLE exhibit excellent performance in hybrid oxide supercapacitor.

Denitrifying woodchip bioreactors are a practical nitrogen (N) mitigation technology but evaluating the potential for bioreactor phosphorus (P) removal is highly relevant given that (1) agricultural runoff often contains N and P, (2) very low P concentrations cause eutrophication, and (3) there are few options for removing dissolved P once it is in runoff. A series of batch tests evaluated P removal by woodchips that naturally contained a range of metals known to sorb P and then three design and environmental factors (water matrix, particle size, initial dissolved reactive phosphorus (DRP) concentration). Woodchips with the highest aluminum and iron content provided the most dissolved P removal (13±2.5 mg DRP removed/kg woodchip). However, poplar woodchips, which had low metals content, provided the second highest removal (12±0.4 mg/kg) when they were tested with P-dosed river water which had a relatively complex water matrix. Chemical P sorption due to woodchip elements may be possible, but it is likely one of a variety of P removal mechanisms in real-world bioreactor settings. Scaling the results indicated bioreactors could remove 0.40 to 13 g DRP/ha. Woodchip bioreactor dissolved P removal will likely be small in magnitude, but any such contribution is an added-value benefit of this denitrifying technology. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-021-15835-w.

This study investigated experimentally pyrolysis of rubberwood sawdust (RWS), sewage sludge (SS), and their blends (25:75, 50:50, and 75:25 by weight) in an agitated bed pyrolysis reactor. The yields and characteristics of liquid product and biochar were determined for pyrolysis at 450, 500, and 550 °C and were affected both by temperature and feedstock type. The liquid and biochar yields were in the ranges 27.30-52.42 and 21.43-49.66 (wt%). Pyrolysis of RWS at 550 °C provided the highest liquid yield, while SS gave a high biochar yield. Co-pyrolysis of SS with RWS improved yield and quality of liquid and biochar products. The liquid product had 57.54-70.70 wt% of water and a low hydrocarbon content. The higher heating value (HHV) of water-free liquid product was 14.73-22.45 MJ/kg. The major compounds of liquid product included acetic acid, 2-propanone, 1-hydroxy, and phenols according to GC-MS. The biochar from RWS had a high carbon content (83.37 wt%) and a high HHV (33.57 MJ/kg), while SS biochar was mainly ash (67.62 wt%) with low carbon content. The SS biochar also had high contents of Si, Ca, Fe, K, and Mg as determined by XRF. Co-pyrolysis of SS with RWS improved the biochar by increasing its carbon content and reducing ash and inorganic elements. The surface of RWS biochar was more porous, while SS biochar had the larger specific surface according to SEM and BET. Based on these results, co-pyrolysis of 75:25 feedstock mix is recommended for further studies on applications of liquid product and biochar.

Objectives: To estimate the annual burden of mortality and the associated health costs attributable to air pollution from wood heaters in Armidale. Design: Health impact assessment (excess annual mortality and financial costs) based upon atmospheric PM2.5 measurements. Setting: Armidale, a regional Australian city (population, 24 504) with high levels of air pollution in winter caused by domestic wood heaters, 1 May 2018 - 30 April 2019. Main outcome measures: Estimated population exposure to PM2.5 from wood heaters; estimated numbers of premature deaths and years of life lost. Results: Fourteen premature deaths (95% CI, 12-17 deaths) per year, corresponding to 210 (95% CI, 172-249) years of life lost, are attributable to long term exposure to wood heater PM2.5 pollution in Armidale. The estimated financial cost is $32.8 million (95% CI, $27.0-38.5 million), or $10 930 (95% CI, $9004-12 822) per wood heater per year. Conclusions: The substantial mortality and financial cost attributable to wood heating in Armidale indicates that effective policies are needed to reduce wood heater pollution, including public education about the effects of wood smoke on health, subsidies that encourage residents to switch to less polluting home heating (perhaps as part of an economic recovery package), assistance for those affected by wood smoke from other people, and regulations that reduce wood heater use (eg, by not permitting new wood heaters and requiring existing units to be removed when houses are sold).

Intenziteta glivnega razkroja je v največji meri odvisna od vrste lesa, temperature in padavinskih dogodkov. Če želimo oceniti življenjsko dobo in intervale vzdrževanja lesenih objektov, moramo oceniti, kako se bo v določenem okolju les obnašal. V ta namen so nam na voljo raznoliki modeli. V praksi se največ uporablja pristop, ki ga je razvil Theodore Scheffer. Scheffer je predlagal klimatski indeks (Schefferjev klimatski indeks - SCI), ki temelji na številu padavinskih dni in povprečni mesečni temperaturi. Na podlagi teh klimatskih podatkov smo izračunali Schefferjev klimatski indeks za izbrane lokacije v Sloveniji. Rezultati analize klimatskih razmer kažejo, da je Schefferjev klimatski indeks v večini analiziranih krajev v Sloveniji višji, kot je bil pred desetletji. Intenziteta razkroja se je povečala v vseh analiziranih krajih z izjemo Portoroža. Največje zvišanje Schefferjevega klimatskega indeksa smo zaznali v Ratečah. Heat islands (HI) are a common anthropogenic phenomenon and are defined as artificial surfaces (urban areas) that have a higher average temperature than their surroundings (rural areas). The aim of this work was to determine the influence of land use on the spatial distribution and intensity (HIi ) of HI in Slovenia. The MODIS Land Surface Temperature (LST) and Corine Land Cover (CLC) databases were used to perform the analysis. Within the identified HI, two HI levels were determined based on temperature difference. The results revealed a statistically significant negative correlation between HIi and both forest cover and forest fragmentation (forest edge density and ratio of mean forest patch size to HI size). Artificial surface was positively correlated with HIi . The results contribute to the understanding of the spatial distribution of HI and HIi and provide informationon for spatial planning and policy-making to reduce the negative impact of HI.

Objectives The aim of this study is to conduct a quantitative assessment of the risk related to occupational exposure to wood dust and the occurrence of sinonasal epithelial cancer (SNEC) by histotype, depending on the duration of exposure, using mathematical models. Material and Methods The relationship between the occurrence of SNEC by histotype (adenocarcinoma [AR] and tumors of other histotypes) and exposure to wood dust has been studied using the values of the odds ratios for individual periods of employment involving exposure to wood dust, and their 95% confidence intervals. The dose-response curves were constructed (more precisely, the duration of exposure-response curves). The author attempted to match the linear, quadratic or exponential models. Results In all SNEC cases, there is a relationship between the duration of occupational exposure to wood dust and the relative risk of developing cancer. The estimated relative risk of developing AR after 35 years of exposure to wood dust is about 300, and the estimated risk of developing SNEC is 50, compared to non-exposed people for whom the relative risk is equal to 1. However, the relative risk of developing other types of cancer is

In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3). In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction. The CNT acts as an extended surface to further improve thermal conductivity. FE-SEM showed that the honeycomb-like pore structure of DP was completely filled with E7U3, E7T3 and CNT. FTIR and XRD analysis show that there is only a combination of physical interactions between the components of FPCMs. DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)). Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles. In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.

Woody cells generate lignocellulosic biomass, which is a promising sustainable bioresource for wide industrial applications. Woody cell differentiation in vascular plants, including the model plant poplar (Populus trichocarpa), is regulated by a set of NAC family transcription factors, the VASCULAR-RELATED NAC-DOMAIN (VND), NAC SECONDARY CELL WALL THICKENING PROMOTING FACTOR (NST)/SND, and SOMBRERO (SMB) (VNS)-related proteins, but the precise contributions of each VNS protein to wood quality are unknown. Here, we performed a detailed functional analysis of the poplar SMB-type VNS proteins PtVNS13–PtVNS16. PtVNS13–PtVNS16 were preferentially expressed in the roots of young poplar plantlets, similar to the Arabidopsis thalianaSMB gene. PtVNS13 and PtVNS14, as well as the NST-type PtVNS11, suppressed the abnormal root cap phenotype of the Arabidopsis sombrero-3 mutant, whereas the VND-type PtVNS07 gene did not, suggesting a functional gap between SMB- or NST-type VNS proteins and VND-type VNS proteins. Overexpressing PtVNS13–PtVNS16 in Arabidopsis seedlings and poplar leaves induced ectopic xylem-vessel-like cells with secondary wall deposition, and a transient expression assay showed that PtVNS13–16 transactivated woody-cell-related genes. Interestingly, although any VNS protein rescued the pendant stem phenotype of the Arabidopsis nst1-1 nst3-1 mutant, the resulting inflorescence stems exhibited distinct cell wall properties: poplar VNS genes generated woody cell walls with higher enzymatic saccharification efficiencies compared with Arabidopsis VNS genes. Together, our data reveal clear functional diversity among VNS proteins in woody cell differentiation and demonstrate a novel VNS-based strategy for modifying woody cell wall properties toward enhanced utilization of woody biomass.

Lignin-decomposing ability of several bacteria and the degradation mechanism have been revealed in vitro. However, the abundance of such bacteria in decayed wood in nature remains unknown at genus and species levels. This study was aimed at identifying bacterial communities in the decayed wood coexisting with white-rot fungi, which play a potential role in lignin degradation, and predicting the functional profile of bacterial lignin degradation in wood via bacterial community analyses. The bacterial flora of forest soil and four decayed wood samples showed marked differences; particularly, in addition to Methylobacterium and Acidibrevibacterium, sphingomonads, which degrade the major skeleton of lignin in vitro, were more abundant in the decayed wood than in forest soil, suggesting that multiple bacteria were involved in lignin degradation. The bacterial community in the decayed wood was more influenced by wood type and lignin structure than the fungal species observed in the decayed wood.