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Brazilin Isolated from Caesalpina Sappan Wood Induces Intrinsic Apoptosis on A549 Cancer Cell Line by Increasing p53, caspase-9, and caspase-3
- Source :
- Asian Pacific Journal of Cancer Prevention. 23:1337-1343
- Publication Year :
- 2022
- Publisher :
- EpiSmart Science Vector Ltd, 2022.
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Abstract
- Lung cancer is the leading cause of death among cancer patients. The majority of lung cancer is the Non-Small Lung Carcinoma (NSLC). This study evaluated the potency of brazilin isolated from Caesalpinia sappan wood to induce apoptosis on non-small lung carcinoma cell line, A549, by examining the expression of p53, caspase-9, and caspase-3.Brazilin was isolated from Caesalpinia sappan wood following a guided assay and it was determined by using Brazilin®SIGMA as standard. The activity of brazilin on the growth of A549 cell line was analysed by MTT assay and the apoptosis was evaluated by flowcytometer following Annexin V (FITC) and PI staining. The expression of p53, caspase-9, and caspase-3 was examined by immunocytochemistry.The IC50 of brazilin on A549 cell line was 43µg/mL. Cell treatment with 20 µg/mL and 40 µg/mL of brazilin significantly increased early apoptosis (p0.001). Cell treatment with 40 µg/mL of Brazilin significantly increased late apoptosis (p0.001). Brazilin significantly increased the expression of p53, Caspase-9, and caspase-3 (p0.001).This study showed evidence of the activity of brazilin to induce intrinsic apoptosis on a NSLC cell line A549.
Details
- ISSN :
- 2476762X
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Cancer Prevention
- Accession number :
- edsair.doi.dedup.....f1841c27206da5a8a4c0c045d2a8fab3