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To determine the chromosomal locations for genes expressed in porcine Peyer's patches, polymerase chain reaction-based mapping of expressed sequence tags (ESTs) isolated from a porcine Peyer's patch-specific cDNA library was performed across a 6500-rad swine radiation hybrid panel. A total of 116 ESTs were mapped with LOD scores >6.0, and another 11 ESTs had LOD scores between 5.0 and 6.0. Of these 127 ESTs, 63% matched known genes (

This article discusses radiation hybrid and comparative mapping of 83 canine brain expressed sequence tags (ESTs). The canine brain ESTs were compared with sequences in the NCBI database with BLAST to find matches to putative orthologous human genes. The human gene locus with the most significant match to each canine ESTs is provided in the article. Three of the eight unlinked canine ESTs were putative orthologues of human X chromosome genes. Amplicons for the six ESTs for which the canine assignment was to an unexpected chromosome were sequenced to confirm their identity.

The article discusses issues related to radiation hybrid and comparative mapping of 38 canine heart expressed sequence tags (ESTs). Random clones from a cDNA library constructed from the right and left atria and ventricles of two mixed breed dogs were sequenced and annotated. Sequences chosen for RH mapping were contigs assembled from two or more individual ESTs from this library. Accession number(s) used to identify primers for polymerase chain reaction amplification of genomic DNA are provided in the article. Statistical analysis for placement of each EST marker was performed by two-point linkage analysis in a two-step process with the Multimap software.