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Essential oil from peels of lemon (Citrus Lumion) fruit had been evaluated for its physiochemical, phytochemical compositions and application in skin-care product. Lemon peel oil, which is one of the under-utilized essential oil was isolated from the matured fruits peel using petroleum ether by soxhlet extraction method. The percentage yield of 3.7 %, for air-dried peels and 2.30 %, for fresh peels are acceptable for most plants essential oils. The ash content of both the dried and fresh peels were 1.42 ± 0.001 and 4.007±0.003. Some phytochemicals like, saponins, flavonoids, terpenes, carbohydrates, tannins, were determined using standard methods. The lemon-peel-oil skin-lotion formulation was carried out by mixing 5 ml of the peel oil sample with corresponding mass of basic lotion compositions, in water base-tank, and homogenously mixed in oil base-tank under 700 C. The pH range(6.30), viscosity, spreadability, and properties of the lotion were compared to the non-lemon-oil lotion and to a commercial grade skin lotion.

Xylanase is an enzyme that acts on xylan and degrades it into xylose. Xylanase may be produced by several different microbes such as bacteria and fungi. Xylanase has significant applications in food industries for improving dough handling and improving the quality of baked products in the extraction of starch, coffee, and plant oils. Xylanase, along with pectinase and cellulose, has enormous application in the clarification of fruit juices. In the present study, xylanase was produced by solid-state fermentation using lemon peel as substrates. Aspergillus Niger was induced to produce xylanase by frequent sub-culturing on a medium containing 2% xylan. OFAT was analyzed by using several fermentation parameters such as moisture content, particle size, incubation period, incubation temperature, peptone concentration, and pH of the extraction buffer. Under optimized conditions, the maximum xylanase activity was found for particle size of 1.7mm, moisture content of 80%, peptone concentration in nutrient solution at 0.3%, and extraction pH of 7.0. Hence, xylanase can be further subjected to down streaming processes and therefore for various applications.

The presence of pectin in the apple peel creates undesirable turbidity and sediment in the final juice and hence clarification is a necessary step for producing consumer-friendly apple juice. This study aimed at evaluating the potential of lemon peel powder as a source of reusable pectinase enzyme in the clarification of apple juice. In this study, optimization of the amount of lemon peel powder addition and incubation time was carried out to produce an apple juice of acceptable clarity. Lemon peel powder as an enzyme source having an activity of 2804.4 U/g could be successfully used up to 5 cycles of pectin hydrolysis. Pectinase enzyme in the lemon peel powder had greater stability at 4 °C with an 11.11% decrease in enzyme activity on 60th day of storage. Treatment with 0.5% w/v lemon peel powder at an incubation time of 90 min was found to be optimum as it produced a clarified apple juice with good sensory acceptability. Lemon peel powder as a naturally immobilized pectinase source was found to be effective in producing clear apple juice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13197-021-05270-7.

Preparation of biscuit incorporating lemon peel powder can be a new approach in formulating fortified fast food. This investigation was made to evaluate quality of prepared biscuits with lemon peel powder. The effect of temperature on drying time and physiochemical properties of lemon peel powder and their effects on biscuits were also examined. Two drying temperatures (650C and 750C) were employed for both blanched and unblanched lemon peel. The formulated biscuit samples incorporating 1 % lemon peel powder were compared with control biscuits which were prepared with 100% wheat flour. It took longer time (18 hr) for drying of blanched sample at 65 0C whereas shorter time (8 hr) was required for that of blanched sample at 750C. There was a significant decrease in the physiochemical properties of lemon peel powder with the increase of temperature except protein and fibre content. In addition, moisture content of the unblanched sample was found to be lower than the blanched sample at the same temperature. Significantly higher ascorbic acid was obtained by the unblanched samples. Protein and fibre content for both unblanched and blanched sample at the same temperature were found to be higher. All the samples were significantly different at 5% level of significance for overall acceptability. Biscuits prepared with unblanched dried lemon peel powder (at 650C) sample secured the highest score in case of all the sensory parameters. Therefore, quality fortified biscuit can be prepared commercially by incorporating 1 % lemon peel powder in wheat flour for the fast food consumer.

The ohmic heating process is known as homogeneous, efficient, and rapid heating. In the present study, it was aimed to analyze the extraction of pectin from lemon peel using ohmic heating, to evaluate the specific pectin production coefficient (SPPC, mg/kJ) and compared it with traditional pectin production (TPP). The extraction process was carried out at 3 different temperatures (70–90 °C), 3 different solid:liquid ratios (1:30, 1:40, 1:50 g/ml), 3 different extraction holding times (0–30 min), and a constant pH (pH 2 that adjusted with citric acid). From the results, extraction conditions affected the pectin yield. As the temperature and extraction holding time increased, the pectin yields increased. In general, the 30-min pectin yields of the ohmic heating-assisted extraction (OHAE) process were similar to the pectin yields obtained from the TPP under the same conditions. Performance evaluation of pectin production was made for 90 °C, and energy efficiency was found to be over 69% for 3 different solid to liquid ratios. It has been stated that as an alternative to the TPP method, the OHAE process can be applied.

Thirty one samples of gum swabs were collected from patients with tooth caries (5-30 years old) from the College of Science (Biology department )- University of Baghdad- Iraq for the period from October 2018 to December 2018. , The samples were transported, after inoculation in a transport media (nutrient broth), to the laboratory of the College of Science and then cultured on mannitol salt agar and blood agar). The isolates belonging to Staphylococcus spp. were identified by biochemical tests and Vitek 2 compact system, while the more antibiotic resistant isolates were identified by using Polymerase Chain Reaction(ï´¾PCR) and sequencing of 16SrRNA . The results showed sharp UV absorption peaks at 330 - 340nm and AFM at 56 nm. Transmission Electron Microscope (TEM) results showed that lemon silver nanoparticles (NPs) are spherical in shape. The results also demonstrated that the silver NPs have a higher antibacterial activity with a diameter of inhibition zone that reached 16mm as compared with the common three mouthwash and toothpaste brands (Colgate, Crest and Close up) that had inhibition zone values of 5-8mm.

The citrus juice industry produces a significant amount of peel residues; it can represent between 18 and 30% of the total weight of the fruit. In recent years, there has been an increase in its use as a source of fiber. The objective of this study was to evaluate the Tahiti lemon peel flour (Citrus latifolia Tanaka) as a fat mimetic (10, 20 and 30%) in a cake. The chemical and nutritional characterization of the lemon peel, the determination of the drying conditions to obtain the flour of the lemon peel, and the physical, chemical, and nutritional characterization of the lemon peel flour and cake was evaluated. A high content of dietary fiber for Tahiti lemon peel (89.15 ± 0.00 g/100 g) and flour (85.30 ± 0.06 g/100 g) was obtained. For the drying conditions to obtain the lemon peel flour, a temperature of 60 °C during 16 h was selected. The cake with greater acceptability had a 10% fat replacement with lemon peel flour, which presented a reduction of 19.16% in the fat content and an approximately double increase in the dietary fiber content. This study suggests that the flour obtained from Tahiti lemon flavedo can be used as a mimetic of fat in cakes, contributing to the nutritional characteristics of the food in which it is included.

Bu çalışmada, hamsi yağına eklenen narenciye kabuğu esansiyel yağlarının (portakal, limon, mandalina ve greyfurt) sprey kurutma yoluyla mikroenkapsüle edilmesi sonucu elde edilen balık yağı tozlarının oda sıcaklığında (24±1oC) 12 haftalık depolama süresi boyunca rengi ve duyusal kalitesi üzerindeki etkisi araştırılmıştır. Mikroenkapsülasyon sonrası örneklerin renk parlaklığı (L*) değerinin 76.46 - 81.51, a* değerinin (-2.50) - (-0.52) ve b* değerinin 7.41 - 19.39 arasında değiştiği görülmüştür. Genel olarak, L*, renk beyazlığı (Whiteness) ve renk tonu (Hue) değerleri depolama süresiyle birlikte azalırken, a *, b * ve renk berrraklık (Croma) değerleri dalgalanma göstermiştir. Gruplar arasında en fazla renk değişimi (∆E) kontrol grubunda (8.04), en az değişim ise mandalina kabuğu esansiyel yağ grubunda (4.55) gözlenmiştir. ME balık yağı tozları koku, görünüm ve partikül çapları bakımından birbirlerinden farklılık göstermiştir. Görsel gözlemler, ME balık yağı tozları kontrol grubunun renginin beyaz, ME portakal ve limon kabuğu EO gruplarının soluk beyaz, ME mandalina kabuğu EO grubunun sarımsı ve ME greyfurt kabuğu EO grubunun şampanya renginde olduğunu göstermiştir. Tüm grupların kuru toz örnekleri ince ve pürüzsüz olmasına rağmen, özellikle kontrol grubunda depolama sonlarına doğru kümelenme- topaklaşma gözlenmiştir. Panelistler tarafından kontrol grubunda gözlenen balık kokusunun, eklenen esansiyel yağlar ile baskılandığı tespit edilmiştir. Aynı zamanda kullanılan esansiyel yağların depolamanın sonlarına doğru kontrol grubunda gözlenen acılaşma kokusunu bastırdığı ve renk sonuçları ile de uyumlu olarak yağların acılaşmasını önlediği dolayısıyla oksidasyonu önlediği sonucuna varılmıştır.

The microwave-assisted extraction of pectin from sweet lemon peel (SLP) was optimized using Box-Behnken design. The highest pectin yield (25.31%) was observed under optimal condition (microwave power of 700 W, irradiation time of 3 min and pH of 1.5). The physicochemical, structural and some bioactivity of the SLP pectin isolated at optimum condition was evaluated. The SLP pectin was rich in galacturonic acid and galactose (87.2 mol%), high in molecular weight (615.836 kDa) and low in degree of esterification (1.2-35.1%). Furthermore, the SLP pectin was composed of 55.7% linear region (homogalacturonan) and 42.2% hairy region (rhamnogalacturonan-I). Also, the FT-IR and H-NMR results confirm the major presence of low methylated galacturonic acid rich structure in the isolated samples. In addition, SLP pectin showed good emulsifying and antioxidant properties. A pseudoplastic flow behavior was observed for SLP pectin solution at higher concentrations (1% w/v). These results represent an inexpensive source for pectin extraction with high pectin yield and good properties.

Pectin extracted via hydrodynamic cavitation in water only from waste lemon peel and further isolated via freeze drying displays significant antibacterial activity against Staphylococcus aureus, a Gram positive pathogen which easily contaminates food. The antibacterial effect of the new IntegroPectin is largely superior to that of commercial citrus pectin, opening the way to advanced applications of a new bioproduct now obtainable in large amounts and at low cost from citrus juice industry's waste., Bacteria vs. lemons! Pectin extracted via hydrodynamic cavitation in water only from waste lemon peel and further isolated via freeze drying displays significant antibacterial activity against Staphylococcus aureus, a Gram positive pathogen which easily contaminates food.

This study was carried out to clear the effect of addition of pomegranate and lemonpeel extracts as natural antioxidant on Feta like cheese quality. These extracts were added to cheeseduring manufacture at a rate of 0.25 and 0.50% of each extract. Cheese treatments were analyzed forchemical analysis, oxidative stability, microbiological examination and organoleptic properties, whenfresh and after 7, 14, 21 and 28 days of storage at refrigerator temperature. Results showed that theseextracts have a high content of phenolic compounds, and gave high antioxidant activity. As well as theaddition of these extracts to cheese did not significantly affect the chemical composition but affectedthe oxidative stability, bacteriological and organoleptic properties of cheese samples. A clear reductionin peroxide and acid values of cheese samples containing natural extracts during storage period thancontrol cheese samples where observed. As well as total bacterial, coliform, as well as yeast andmould counts of cheese samples contain natural extracts did not detected during storage comparedwith control cheese samples. Also, results showed that organoleptic properties of all cheese treatmentsimproved by progressed of storage period until the end of storage. Cheese containing lemon peelsextracts showed lowest flavour intensity and body characteristics than other cheese. From the previousresults, it could be recommended the use of some natural extracts in feta like cheese manufacture suchas pomegranate and lemon peel extracts at a rate of 0.5%, where it improved the sensory andbacteriological characteristics of cheese samples and increased stability against oxidation.

To provide stable and low-cost naturally derived yellow pigments, a variety of food byproducts were evaluated and the constituents of lemon peel have emerged yielding a highly promising natural product with applications as a food dye. Here we report a new, highly stable and water soluble food dye called yellow #15 from the ethanol extract of the zest of Citrus limon. The structure of lemon yellow #15 was carefully assigned on the basis of spectroscopic data, including 1D and 2D NMR spectroscopy, and the absolute configuration was established by comparison of the experimental CD with calculated electronic circular dichroism (ECD) spectral data. CIELAB values and Delta CIELAB were measured and revealed this new water-soluble pigment has superior light stability relative to other natural products used as food dyes.

Lemon peel can be used as traditional Chinese medicine. Flavonoids are the most important components in lemon peel, which can be developed as natural medicine without side effects. This study investigated the protective effect of lemon peel polyphenols (LPP) on human keratinocyte HaCaT cells under oxidative stress. The active components of LPP were determined by high performance liquid chromatography. The abilities of LPP to scavenge DPPH and ABTS+ free radicals were studied for detection of antioxidation in vitro. Cell survival rates were determined by MTT assay. The antioxidant enzyme activity and antioxidant index of cells were determined using kit. The mRNA and protein expression of cells were determined by qPCR and western blot. The ability of LPP to scavenge DPPH and ABTS+ free radicals were stronger than those of vitamin C (Vc) at the same concentration. As expected, compared with the normal group of cells, the model group had decreased cell survival, increased lactate dehydrogenase (LDH), decreased levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), and increased malondialdehyde (MDA) content. qPCR and western blot results indicated that the expression of Bcl-2-related X protein (Bax), caspases-3, erythroid-derived nuclear factor 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) were decreased and the expression of B-cell lymphoma-2 (Bcl-2) was increased in the model group, compared with the normal group. LPP treatment improved cell survival rate, reduced intracellular LDH and MDA levels, increased intracellular SOD, CAT, GSH levels, down-regulated Bax, caspases-3, Nrf2, HO-1 expression, and up-regulated Bcl-2 expression. Component analyses found that LPP contains gallic acid, neochlorogenic acid, (+)-catechin, caffeic acid, (−)-Catechin gallate, isochlorogenic acid A, rosmarinic acid, and protocatechuic acid. LPP was found to regulate the Nrf2/HO-1 signaling pathway through 8 active substances to protect HaCaT cells against oxidative stress in vitro.

This study aimed to evaluate the significant extraction factors in achieving higher recovery yield of total polyphenols, phenolic acids and flavonoids content from Citrus limon peel using two-level factorial design. The effect of five independent factors including drying temperature (40–60 °C), methanol concentration (20–60%), extraction temperature (28–60 °C), extraction time (30–60 min) and storage duration (0–14 days) were evaluated. Among all the examined factors, results showed that drying temperature, storage duration and extraction temperature were the most significant and contributing factors affecting the total polyphenols, phenolic acids and flavonoids content of lemon peel at P

Citrus limon (L.) Burm is an important crop that grows between latitudes 30&deg, North and 30&deg, South, the main producers being China, the USA, Mexico, India, Brazil, and Spain. In Spain, lemon grows mainly in Mediterranean areas such as Murcia, Valencia, and Andaluc&iacute, a. The most cultivated varieties are &ldquo, Fino&rdquo, and &ldquo, Verna&rdquo, In this study, five varieties of lemon, &ldquo, &ldquo, B&eacute, tera&rdquo, Eureka&rdquo, Fino 49&rdquo, Fino 95&rdquo, were evaluated on different rootstocks: three new Forner-Alcaide (&ldquo, FA13&rdquo, FA5&rdquo, FA517&rdquo, ), Citrus macrophylla, Wester, and Citrus aurantium L. Hydrodistillation was used to obtain essential oil from fresh peels and then the volatile profile was studied by gas chromatography-mass spectrometry (GC-MS). A total of 26 volatile compounds were identified, limonene being the main one followed by &beta, pinene, &gamma, terpinene, sabinene, and &alpha, pinene. The results revealed that Forner-Alcaide rootstocks (&ldquo, &gt, ) proved to be the best rootstocks for the aroma quality as they led to high volatile contents, followed by C. aurantium and C. macrophylla. Among the other varieties, the most aromatic one was &ldquo, The whole trend was as follows (in decreasing order): &ldquo

The aim of this study was to investigate the effect of material type (artichoke leave, lemon peel, flaxseed meal), extraction temperature (50, 100, 120, 140, 160, 180, 200 °C) and static extraction time (5, 15, 30, 45 min) on 5-hydroxymethylfurfural (5-HMF) formation during subcritical water extraction. 5-HMF content of artichoke leave and lemon peel extracts increased 7.2 and 26.1 times with the rise of extraction temperature from 160 to 180 °C for 5 min during subcritical water extraction, respectively. Besides, 5-HMF content of artichoke leave, lemon peel and flaxseed meal extracts increased 1.4, 2.0 and 4.5 times as static extraction time increased from 15 to 45 min at 180 °C during subcritical water extraction, respectively. The highest 5-HMF content of artichoke leave and lemon peel extracts were obtained as 58.83 and 231.21 mg/L at 180 °C and 45 min, respectively. However, for flaxseed meal, the highest 5-HMF content (222.94 mg/L) was obtained at 200 °C and 15 min during subcritical water extraction.

The antibacterial effect of citrus peel lemon against the bacterial strains obtained from the filters of air conditioners have been selected based on the presence most in these filters such as Streptococcus, Bacillus spp, Pseudomonas, E coli. agar well diffusion method used to evaluate antibacterial activity of citrus peels water extract. through the results became clear to us that the cold aqueous extract of lemon peel showed a significant effect on the growth of bacterial species through the diameters of inhibition zone that appeared in all concentrations of the extract (125, 250, 500, 1000) mg/ml. Gram-positive bacteria Streptococcus spp were the most affected Where the diameters of inhibition zone (18, 15, 12, 0, 0) mm respectively, while The Gram-negative bacteria E coli least affected. The results obtained in this study indicate that citrus lemon peel can be used in the treatment of diseases caused by organisms for the purposes of the pharmaceutical.

In this study, functionalized biscuits were prepared through the enrichment of dough with lemon peel and natural antioxidants extracted from lemon pomace. Lemon pomace extract (LPE) was analyzed for total phenolic content before addition, and then a known concentration of 50 mg kg−1 was used for the formulation of enriched biscuits. Three different biscuit samples were compared to a control biscuit, without the addition of functional ingredients. The main physicochemical, microbiological, and sensory aspects of doughs and biscuits enriched with LPE were investigated. The enriched biscuits showed higher phenolic content and antioxidant activity than the control one and a longer induction period (IP), which means that the enriched products had a higher intrinsic resistance to lipid oxidation, thanks to the antioxidant effect exerted by the added fresh lemon peel and the LPE. Furthermore, from a sensorial point of view, they showed suitable acceptability, in terms of appearance, flavor, and aromatic attributes. Thus, results indicated that the incorporation of lemon processing by-products allowed the production of functional enriched biscuits with improved antioxidant properties.

Soy protein with essential oil of lemon peel was used to produce a biodegradable film, which showed inhibition activity against some species of selected bacterial strains. The films were white cheese coating and studied for its biodegradation in the soil. The percentage of soy protein extract was 15% (W: W) while the percentage of lemon peel essential oil was 3% (V: W). The GC-MS result of lemon peel essential oil consisted of 38 compounds, of which the main compound is D-Limonene in 63.43%. The films produced showed inhibition activity against both Gram-positive and Gramnegative bacteria. Inhibition activity was highest against Staphylococcus aureus (22 mm zone), while E. coli O157: H7 had the lowest inhibition (18mm) when 0.1mL of essential oil with 10mL film mixed. The coating process of white cheese reduced the total microbial population (CFU/g) during 30 days of storage time. The TCB, TC, ST and MY of cheese coating were 4.28, 1.53, 1.62 and 1.33 log. CFU/g respectively after 30 days compared to the cheese non-coating samples, with 6.53, 2.51, 3.17 and 1.81 log. CFU/g, respectively. The biodegradation in soil was 97 and 72% for soy protein film and soy protein film with lemon peel essential oil after 35 days.

The influence of natural, hot-air and infrared drying on chemical composition and bioactivity of lemon peel essential oil are investigated in this study. The results showed that drying resulted in losses or increases of some components or production of some new substances, but the d-limonene (59.52–70.01%) was found as the main component of essential oil. Drying brought about decreases in the yield, antioxidant and antibacterial activity of essential oil. However, the natural drying had little effect, while the hot-air and infrared drying resulted in significant decreases in these parameters, especially at the higher temperature. The yield was the lowest under hot-air drying (60 °C) and decreased by 78%, while infrared drying (60 °C) sample exhibited the lowest antioxidant and antibacterial activities. Infrared drying was easier to lead to the decrease in bioactivity than hot-air drying at the same temperature. These results provided the theoretical basis for drying lemon peel.

This study aimed to investigate the effect of low-pressure dielectric barrier discharge (DBD) plasma on microwave-assisted hydrodistillation of lemon peel oil extraction. Microwave pre-treated lemon peel powder was exposed to plasma treatment (1.0, 1.5, 2.0, and 2.5 kV) for 10 min. The treated lemon peel powders were subjected to hydrodistillation to extract the essential oil and the extraction yields were calculated. The extracted oil was analyzed for chemical composition with gas chromatography-mass spectrometry (GC-MS). Effect of plasma on the surface morphology of the lemon peel was observed in a scanning electron microscope (SEM) which revealed the formation of fissures and cracks owing to the higher extraction yield. Plasma treatment at 2.5 kV was observed higher extraction yield than conventional hydrodistillation (149.34 % rise) and the chemical composition of plasma treated sample essential oil remains significantly unchanged. Thus, DBD plasma could be a promising technique to enhance the lemon peel essential oil extraction.

Current application of coffee pulp into tea, called as cascara tea, had improved its usage but it might have an unpleasant aroma and taste. Thus, the addition of other resources such as lemon peel is suggested. This study aims to know the effect of brewing techniques and the ratio of cascara (dried coffee pulp) and lemon peel to physicochemical and organoleptic characteristics of cascara tea, along with the best treatment. This study was conducted by Nested design with two factors, i.e. brewing techniques and the ratio of cascara and lemon peel. The data were analyzed using Minitab 17 and Zeleny method. The results showed that brewing techniques had a significant effect on total phenolic content, caffeine content, color (except in yellowness) and pH. Meanwhile for both factors had a significant effect on all parameters. The best treatment was the ratio of cascara and lemon peel at 80:20% brewed using the decoction method.

This study examines the ameliorative effects of lemon (Citrus limon) peel (LP) powder on intestinal inflammation and barrier defects in dextran sulfate sodium (DSS)-induced colitic mice. The whole LP powder was fractionated into methanol (MetOH) extract and its extraction residue (MetOH residue), which were rich in polyphenolic compounds and dietary fibers, respectively. Mice were fed diets containing whole LP powder, MetOH extract, and MetOH residue for 16 d. DSS administration for 9 d induced bodyweight loss, reduced colon length, reduced the colonic expression of tight junction proteins including zonula occludens-1 and -2, and claudin-3 and -7, and upregulated colonic mRNA expression of interleukin 6, chemokine (C-X-C motif) ligand 2, and C-C motif chemokine ligand 2. Feeding LP powder restored these abnormalities, and the MetOH residue, but not MetOH extract, also showed similar restorations. Feeding LP powder and MetOH residue increased fecal concentrations of acetate and n-butyrate. Taken together, LP powder reduced intestinal damage through the protection of tight junction barriers and suppressed an inflammatory reaction in colitic mice. These results suggest that acetate and n-butyrate produced from the microbial metabolism of dietary fibers in LP powder contributed to reducing colitis.

Sugar beet pulp (SBP) and lemon peel wastes (LPW) were used to obtain two mixtures of pectic oligosaccharides (denoted as SBPOS and LPOS, respectively). Oligogalacturonides in LPOS showed a larger molecular weight, higher degree of methylation and lower degree of acetylation than the ones in SBPOS. The suitability of pectic oligosaccharides, pectins from SBP and LPW and commercial FOS for causing prebiotic effects were compared by in vitro fermentation and fluorescence in situ hybridization using human faecal inocula and eight different probes. The joint populations of bifidobacteria and lactobacilli increased from 19% up to 29%, 34% and 32% in cultures with LPOS, SBPOS and FOS, respectively. Faecalibacterium and Roseburia also increased their counts with all the substrates (especially with LPOS). The highest concentrations of organic acids were observed in media containing oligosaccharides. This work confirms that pectic oligosaccharides present better prebiotic properties than pectins, and similar or better than FOS.

The principal objective of this study was to appraise the variation of antioxidant activity, total phenols, and total flavonoids of fresh lemon peel from regions located Marrakech and Kenitra in Morocco and a region Taiz in Yemen. The test of polyphenol was performed from extracts that were measured using the Folin-Ciocalteu technique. Also, an analysis of Flavonoid was performed of was measured by using the aluminum chloride. The Free radical scavenging activity of lemon extracts was measured by 2,2′-Diphenyl-1-picrylhydrazyl hydrate (DPPH).The DPPH radical scavenging activity of ethanolic extract of fresh lemon peel found to be highest at 200μl concentration, which was 77. 15 %; 67. 88 %; 37.74%. Through it, 50% inhibition (IC50) was obtained to be 92. 04; 153. 55 and 215.407 μg/ml in Marrakech, Kenitra, and Taiz, respectively. As well, the values radical scavenging activity of methanolic extract of the same concentration, which are 82, 77 %, 53, 33 %, and 47,5%. Through it, 50% inhibition (IC50) was obtained to be 145. 6; 179. 17 and 274.899 μg/ml in Marrakech, Kenitra, and Taiz, respectively. But regarding the values radical scavenging activity of essential oil of the same concentration, which is 63.77 %, 42.19 %, and 80.25%. Through it, 50% inhibition (IC50) was obtained to be 155.54; 271.158 and 101.31 μg/ml in Marrakech, Kenitra, and Taiz, respectively. According to the results obtained in our study, the antioxidant activity increases with increased polyphenols and IC50 deficiency in Marrakech, Kenitra, and Taiz.

The viscoelastic properties of high pressure homogenized lemon peel cell wall fiber suspensions, obtained after sequential selective pectin extraction, were investigated in the current study. For comparison, a general pectin extraction was additionally performed on lemon peel under acid thermal conditions. The obtained insoluble residues and solubilized pectin fractions were characterized based on their neutral sugar and galacturonic acid content, degree of methoxylation and molar mass distribution. Subsequently, microstructure and viscoelastic characteristics of the diverse insoluble residue suspensions were investigated by means of microscopic visualization, determination of the particle size distribution and rheological properties after high pressure homogenization. Due to the use of different extraction conditions, the insoluble cell wall residues were found to have diverse pectin characteristics and contents. While homogenization of non- or moderate-depleted cell wall suspensions resulted in small viscoelastic moduli due to the disruption of the stiff microfibrillar cell wall network, extensive pectin removal was found to correlate to large viscoelastic moduli of the cell wall suspension upon high pressure homogenization. It is suggested that an extensive pectin extraction resulted in the creation of interfibrillar voids within the cellulose-hemicellulose network, increasing the cell wall flexibility and hydration of the fibers upon suspension. Due to the increased flexibility of this fiber network, the interfibrillar voids in the pectin depleted cell wall material were proposed to expand with increasing pressure level during high pressure homogenization of the generated cell wall suspensions, thereby increasing its viscoelastic properties. ispartof: Food Hydrocolloids vol:72 pages:358-371 status: published

Aspergillus flavipes FP-500 grew up on submerged cultures using lemon peel as the only carbon source, developing several batch and pulsed fed-batch trials on a stirred tank reactor. The effect of carbon source concentration, reducing sugar presence and initial pH on exopectinase and endopectinase production, was analyzed on batch cultures. From this, we observed that the highest substrate concentration favored biomass (X max) but had not influence on the corresponding specific production (q p) of both pectinases; the most acid condition provoked higher endopectinase-specific productions but had not a significant effect on those corresponding to exopectinases; and reducing sugar concentrations higher than 1.5 g/L retarded pectinase production. On the other hand, by employing the pulsed fed-batch operation mode, we observed a prolonged growth phase, and an increase of about twofold on endopectinase production without a significant raise on biomass concentration. So, pulsed fed-batch seems to be a good alternative for obtaining higher endopectinase titers by using high lemon peel quantities without having mixing and repression problems to the system. The usefulness of unstructured kinetic models for explaining, under a theoretic level, the behavior of the fungus along the batch culture with regard to pectinase production was evident.

This study evaluated the effect of high pressure processing on the recovery of high added value compounds from citrus peels. Overall, the total phenolic content in orange peel was significantly (P < .05) higher than that in lemon peel, except when pressure treated at 500 MPa. However, lemon peel demonstrated more antioxidant activity than orange peel. Pressure-treated samples (300 MPa, 10 min; 500 MPa, 3 min) demonstrated higher phenolic content and antioxidant activity comparatively to the control samples. For more severe treatments (500 MPa, 10 min), the phenolic content and antioxidant activity decreased in both lemon and orange peels.

Lemon (Citrus limon) is the third most important species of citrus in the world, while Spain is the major producer in Europe. Numerous beneficial effects of lemon are known, which explains their use in traditional medicine. The paper describes the effect of dietary dehydrated lemon peel (a sub-product of the lemon industry) on the growth, immune and antioxidant status of gilthead seabream (Sparus aurata L.) over a period of 30 days. Fish fed diets enriched with dehydrated lemon peel (1.5% and 3%) for 15 days showed improved growth and both humoral (seric immunoglobulin M) and cellular (peroxidase activity and phagocytic ability of head kidney leucocytes) immunity, as well as the expression of some immune-related genes (nkefa, il1β, igth and csfr1). However, decreases growth promotion was observed after thirty days of trial. Neither the anti-oxidant enzymes activity nor the expression of several anti-oxidants and anti-stress genes in liver was improved by the diet. The possible inclusion of dehydrated lemon peel in fish diets for its immunostimulant effects is discussed.

Considering that the determination of authenticity and of the geographical origin of food is a very challenging issue, in this study we studied by means of histological and histochemical analyses the famous Sicilian lemon known as ‘Interdonato Lemon of Messina PGI’. Since the protected geographical indication Interdonato lemon of Messina possesses high organoleptic properties, the composition of the hexane extract of lemon peel was determined by HRGC and HRGC–MS analyses and compared with that of lemon of different cultivars. The results obtained are informative of the oil’s quality and explain the variation of the lemon essential oil composition. Given the fundamental economic implications of any fraud, the aim of this study was to determine a fingerprint able to evaluate the authentication of the geographic origin in such way to prevent frauds in national and international markets.

Citrus peels, the waste by-products of citrus juices factories are reckoned valuable healthful functional food. The present investigation performed to assess nutritional status of wheat and fortified biscuits with citrus peels powders. Study included determination of gross chemical composition, caloric value , minerals (Fe, Mn, Cu, Ca, Mg, Na, K and P) of wheat and fortified biscuits with citrus peels powders. Likewise physical and sensory characteristics of control and 10% fortified wheat biscuits with the four studied citrus peels powders: Tangerine peel powder, Abo-Sora peel powder, Baladi orange peel powder, and Baladi lemon peel powder. The data revealed that 10% incorporation of citrus peels powders in wheat biscuits increased crude protein, crude fat contents as well as crude fiber, moisture contents and caloric value. However, it decreased carbohydrate content. The supplementation of control biscuits with 10% citrus peels powders of the four studied citrus enhanced the nutritive value. The data revealed that all 10% fortified biscuits with the four studied citrus peels powders improved all studied sensory characteristics in all the studied biscuits. Moreover, biscuits fortified with 10% citrus peel powders. Could be recommended for caloric reduced diets for obese, over-weight persons and diabetic persons.

This work studies the optimization of the enzymatic peeling of Primofiore lemons, focusing on determining the changes that happen in the peel albedo. In this process, the conditions of temperature, time and concentration of the enzymatic preparation were determined in order to produce the maximum peel mass loss related to the best peeling efficiency. In this study, the effect of the temperature on the peeling process rate, the kinetic constants at different temperatures and the activation energy of the degradation of the peel albedo were determined. PRACTICAL APPLICATIONS The process described in this work is applied in the peel removal technology by enzyme infusion, being one of the stages in the preparation of minimally processed lemons.

Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sources-supplemented medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R. microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had optimum temperature at 65 ºC and was totally stable at 55 ºC for 90 min. Half-life at 70 ºC was 68 min. These results suggested that the versatility of waste carbon sources utilization by R. microsporus, produce pectic enzymes, which could be useful to reduce production costs and environmental impacts related to the waste disposal.

Lemon peel constitutes a potential source of dietary fiber to formulate new and healthier products, as well as a source of essential oils. The relationship between moisture content and water activity provides useful information for lemon peel processing, especially for drying and storage. Water sorption isotherms of lemon peel were obtained using a standardized conductivity hygrometer at four different temperatures (20, 30, 40 and 50 °C) and wide ranges of moisture content (5.381–0.002 kg water/kg dry solid) and water activity (0.984–0.106). One theoretical (GAB) and four empirical equations (Oswin, Henderson, Halsey and Ratti) were used for modelling sorption isotherms. After evaluating the models according to several criteria, the GAB model appeared as the best option. Isosteric heats of sorption were assessed from experimental sorption isotherm data using different methods.

Imazalil is one of the most widespread fungicides used for the post-harvest treatment of citrus species. The separate use of peel during food preparation and processing may hitherto concentrate most of the imazalil into food products, where specific maximum residue limits hardly exist for this fungicide. In order to monitor comprehensively the path of imazalil, our study covered the monitoring of the efficiency of several washing treatments, the comparison of operative and related sample preparation methods for the lemon samples, the validation of a sample preparation technique for a fatty cake matrix, the preparation of a model cake sample made separately either with imazalil containing lemon peel or with imazalil spiking, the monitoring of imazalil degradation into α-(2,4-dichlorophenyl)-1H-imidazole-1-ethanol because of the baking process, and finally the mass balance of imazalil throughout the washing experiments and the baking process. Quantification of imazalil was carried out with an LC-ESI-MS/MS set-up, while LC-QTOF was used for the monitoring of imazalil degradation. Concerning the washing, none of the addressed five washing protocols could remove more than 30% of imazalil from the surface of the lemon samples. The study revealed a significant difference between the extraction efficiency of imazalil by the EN 15662:2008 and AOAC 2007.1 methods, with the advantage of the former. The use of the model cake sample helped to validate a modified version of the EN 15662:2008 method that included a freeze-out step to efficiently recover imazalil (>90%) from the fatty cake matrix. The degradation of imazalil during the baking process was significantly higher when this analyte was spiked into the cake matrix than in the case of preparing the cake with imazalil-containing lemon peel (52% vs. 22%). This observation calls the attention to the careful evaluation of pesticide stability data that are based on solution spiking experiments.

Summary The sensory attributes of cakes, containing a greater number of low sugar raisins (LSR) than that normally used, were evaluated by two different groups of trained and untrained panellists. The purpose of this sensory evaluation was to find out whether such bakery products were more attractive to consumers than normal cakes and could be further promoted in the market. Cakes containing LSR and additional lemon peel scored more poorly than cakes containing only raisins, both in terms of taste and overall preference. Cakes containing only LSR had good sensory characteristics and could be considered as competitive products when compared with similar products already on the market. Young people found cakes with raisins tasty, but they did not consume them readily. The special advantages of these cakes are their natural ingredients, raisins with lower amount of sugars, good taste and high quality, all of which should be emphasized to make them attractive in the market.

We prepared fermented lemon peel (FLP) with high antioxidative activity by fermentation of the lemon peel (LP) with Aspergillus saitoi. The antioxidative activity of LP and FLP was examined by the radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) in vitro, and the suppressive effect against exercise-induced oxidative damage in rat liver. FLP exhibited higher radical-scavenging activity against DPPH than LP. The administration of LP and FLP prior to exercise significantly suppressed the increases in thiobarbituric acid-reactive substance caused by lipid peroxidation during exhaustive exercise, with FLP having a tendency of higher activity than LP. Moreover, FLP showed a significant suppressive effect of Ne-(hexanonyl)lysine content, a primary oxidative stress marker, which increases due to exhaustive exercise, whereas LP did not. The fermentation of LP with A. saitoi was thought to produce a high antioxidative activity. The high antioxidative activity of FLP was assumed to be related to the production of antioxidative flavonoids, as a hydroxyflavanone and aglycones, by the fermentation of lemon peel with Aspergillus saitoi.

Deuterium-labelled (methyl-d3) pectin was prepared by esterifying a lemon peel pectin with methanol-d4. A pure powder sample and multicomponent model confectioneries containing the labelled pectin and various amounts of water were studied by solid-state 2H NMR spectroscopy. In the pure sample the broad 2H NMR lineshape indicates that the only fast motion occurring on the NMR timescale is reorientation of the methyl-d3 group about its C3 axis. In contrast, in systems of 50% water content the 2H NMR lineshape is much narrower, indicating significant motional averaging. As the percentage water in the confectionery systems is reduced to

The aim of this work was to compare some in uitro health-promoting properties in fruit dietary fibre (antioxidant activity, fermentability and glucose retardation index), which could be useful to predict their physiological effects better than physico-chemical analyses. These properties were evaluated in mango and lemon peel fibre. Mango fibre was better than lemon fibre because of the highest values of antioxidant activity (67.6%) and glucose retardation index (21.5%). Antioxidant activity is proposed as a new health-promoting property associated to dietary fibre.

Furanocoumarins (FCs) such as bergamottin (BG) and 6',7'-dihydroxybergamottin (DHBG) contained in grapefruits are known to be cytochrome P450 3A4 (CYP3A4) inhibitors. These are contained in larger quantity in peel than in pulp, and therefore, processed peel products possibly have strong CYP3A4 inhibitory activity. The CYP3A4 inhibitory potency of these processed peel products, however, remains to be elucidated. The FC content and CYP3A inhibitory activities of various processed fruit peel products were investigated. CYP3A inhibitory activities of crystallized grapefruit peel, grapefruit marmalade, lemon peel and bitter orange slice were close to that of 100% grapefruit juice, while the activities of yuzu slice, pomelo (buntan) marmalade and crystallized iyokan peel were very weak, 1/8-1/20 of 100% grapefruit juice. The maximum BG content was 5.6 µg/g in lemon peel. The maximum DHBG content was 7.2 µg/g in crystallized grapefruit peel, about 1/30 that of raw peel. Grapefruit marmalade and crystallized grapefruit peel contained similar amounts of FCs to 100% grapefruit juice, but FCs were not detected in pomelo (buntan) marmalade or crystallized iyokan peel. Good correlation (r=0.78) was observed between the FC contents of these peel products and those CYP3A inhibitory activities. Preparation of homemade grapefruit marmalade and crystallized peel revealed that considerably lower DHBG content in these products and lower CYP3A inhibitory activity than anticipated were attributable to outflow of DHBG to broth during boiling of the raw peel.

Studies on the viscosity behavior were performed with 20 different spices or dried vegetables. In nine spices (cinnamon, ginger, mustard seed, celery, onions, shallots, lemon peel, black and white pepper) differences between unirradiated and irradiated samples were observed. Further lots were investigated to estimate the variations of viscosity depending on the origin of the samples. Additional storage experiments showed that measuring the viscosity may be a simple method to identify some radiation treated spices even after years.