1. A multispecies Lactobacillus-and Bifidobacterium-containing probiotic mixture attenuates body weight gain and insulin resistance after a short-term challenge with a high-fat diet in C57/BL6J mice
- Author
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Angèle Guilbot, Christian Seyrig, Charlotte Guigné, Marc Dubourdeau, Sandrine Mouysset, Gerald Chene, Sophie Holowacz, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Groupe PiLeJe (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Université Toulouse - Jean Jaurès - UT2J (FRANCE), Université Toulouse 1 Capitole - UT1 (FRANCE), Ambiotis (FRANCE), Institut de Recherche en Informatique de Toulouse - IRIT (Toulouse, France), Institut National Polytechnique de Toulouse - INPT (FRANCE), Groupe PiLeJe, Ambiotis (Ambiotis), Algorithmes Parallèles et Optimisation (IRIT-APO), Institut de recherche en informatique de Toulouse (IRIT), Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse 1 Capitole (UT1), and Université Fédérale Toulouse Midi-Pyrénées
- Subjects
medicine.medical_specialty ,030309 nutrition & dietetics ,Biotechnologie ,Adipose tissue ,Adipokine ,030209 endocrinology & metabolism ,Biology ,law.invention ,Leukotriene-A4 hydrolase ,03 medical and health sciences ,chemistry.chemical_compound ,Probiotic ,0302 clinical medicine ,Insulin resistance ,law ,C57/BL6J mice ,Internal medicine ,medicine ,Pharmacology (medical) ,2. Zero hunger ,Pharmacology ,Inflammation ,0303 health sciences ,Leukotriene ,Leukotriene C4 ,Adiponectin ,Probiotics ,High fat diet ,Body weight gain ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,medicine.disease ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Endocrinology ,chemistry ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Food Science - Abstract
International audience; A multispecies Lactobacillus- and Bifidobacterium-containing probiotic mixture significantly reduced the adverse metabolic and inflammatory effects of a 14-week high-fat diet in wild-type C57/BL6J mice gavaged 5 days a week with the probiotic mixture or vehicle. Recent evidence indicates that the gut microbiome may play a decisive role in the onset of obesity and associated chronic metabolic diseases, such as type 2 diabetes, by modulating nutrient absorption and factors conducive to development of a persistent low-grade inflammatory state. By modifying the gut microbiome, probiotics might constitute an effective dietary strategy for managing these metabolic disorders. The tested probiotic mixture significantly attenuated the increase in body weight, serum glucose concentration and insulin resistance induced by the high-fat diet. Furthermore, it significantly reduced the up-regulation of expression of several genes encoding pro-inflammatory adipokines and leukotriene pathway enzymes (CCL-2, IL-6 and leukotriene C4 synthase in adipose tissue, leukotriene C4 synthase and leukotriene A4 hydrolase in the gut). It also significantly counteracted the down-regulation of adipose tissue gene expression related to the anti-inflammatory adipokine adiponectin in mice fed the high-fat diet. These results suggest that the mechanism underlying the beneficial metabolic effects of the probiotic mixture might involve inhibition of gut and adipose tissue inflammation.
- Published
- 2015