29 results on '"Chengfen Xing"'
Search Results
2. Property Regulation of Conjugated Oligoelectrolytes with Polyisocyanide to Achieve Efficient Photodynamic Antibacterial Biomimetic Hydrogels
- Author
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Hongbo Yuan, Chengfen Xing, Gao Mengshi, Dong Gao, Xiaoning Liu, Changsheng Du, and Bing Wang
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Staphylococcus aureus ,Materials science ,Light ,Polymers ,Nanotechnology ,Microbial Sensitivity Tests ,Thiophenes ,macromolecular substances ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,complex mixtures ,01 natural sciences ,Biomimetic Materials ,Thiadiazoles ,Escherichia coli ,General Materials Science ,Fluorescent Dyes ,Photosensitizing Agents ,technology, industry, and agriculture ,Hydrogels ,Production efficiency ,Fluoresceins ,021001 nanoscience & nanotechnology ,Biocompatible material ,Anti-Bacterial Agents ,0104 chemical sciences ,Self-healing hydrogels ,Reactive Oxygen Species ,0210 nano-technology - Abstract
Fabricating antibacterial hydrogels with antimicrobial drugs and synthetic biocompatible biomimetic hydrogels is a promising strategy for practical medical applications. Here, we report a bicomponent hydrogel composed of a biomimetic polyisocyanopetide (PIC) hydrogel and a photodynamic antibacterial membrane-intercalating conjugated oligoelectrolyte (COE). The aggregation behavior and aggregate size of the COEs in water can be regulated using the PIC hydrogel, which could induce COEs with higher reactive oxygen species (ROS) production efficiency and increased association of COEs toward bacteria, therefore enhancing the antibacterial efficiency. This strategy provides a facile method for developing biomimetic hydrogels with high antibacterial capability.
- Published
- 2021
3. Enhancing hydrogen production by photobiocatalysis through Rhodopseudomonas palustris coupled with conjugated polymers
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Hao Geng, Chengfen Xing, Dong Gao, and Zijuan Wang
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biology ,Renewable Energy, Sustainability and the Environment ,Electron donor ,02 engineering and technology ,General Chemistry ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,Electron transfer ,chemistry ,Polythiophene ,General Materials Science ,Photosynthetic bacteria ,Rhodopseudomonas palustris ,0210 nano-technology ,Hydrogen production - Abstract
Utilizing solar energy for hydrogen production by combining light-activated materials and biocatalysts has become a promising alternative to fossil fuels. Herein, a feasible and simple bio-hybrid complex based on water-soluble oligofluorene (OF), polythiophene (PTP) and Rhodopseudomonas palustris (R. palustris), one kind of photosynthetic bacteria, was constructed for enhancing photocatalytic hydrogen production. Under the irradiation of visible light, there was fluorescence resonance energy transfer (FRET) between OF and PTP, which amplified photoelectron signals and transferred electrons via PTP to methyl viologen (MV2+), an electron mediator that established electron transfer pathways between organic materials and R. palustris. Hydrogen production involving R. palustris was catalyzed by nitrogenase. It is noteworthy that MV2+ was reduced to MV+˙ and transferred electrons to nitrogenase in R. palustris, converting protons and electrons to molecular hydrogen. Besides, triethanolamine (TEOA) was added as an electron donor to provide electrons to PTP. The proposed OF/PTP/MV2+/TEOA/R. palustris system provided a feasible strategy for photocatalytic hydrogen production with low-cost and easily implemented techniques.
- Published
- 2021
4. Biomimetic Networks with Enhanced Photodynamic Antimicrobial Activity from Conjugated Polythiophene/Polyisocyanide Hybrid Hydrogels
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Yong Zhan, Chengfen Xing, Paul H. J. Kouwer, Hongbo Yuan, and Alan E. Rowan
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Absorption (pharmacology) ,Antifungal Agents ,Polymers ,Microbial Sensitivity Tests ,Thiophenes ,Conjugated system ,Catalysis ,law.invention ,chemistry.chemical_compound ,Magazine ,Biomimetic Materials ,law ,Candida albicans ,Escherichia coli ,chemistry.chemical_classification ,Cyanides ,Molecular Materials ,Hydrogels ,General Chemistry ,Polymer ,General Medicine ,Anti-Bacterial Agents ,Photochemotherapy ,chemistry ,Chemical engineering ,Self-healing hydrogels ,Polythiophene ,Antibacterial activity ,Ethylene glycol ,Bacillus subtilis - Abstract
Hybrid biomimetic hydrogels with enhanced reactive oxygen species (ROS)-generation efficiency under 600 nm light show high antibacterial activity. The hybrid gels are composed of helical tri(ethylene glycol)-functionalized polyisocyanides (PICs) and a conformation-sensitive conjugated polythiophene, poly(3-(3 '-N,N,N-triethylammonium-1 '-propyloxy)-4-methyl-2,5-thiophene chloride) (PMNT). The PIC polymer serves as a scaffold to trap and align the PMNT backbone into a highly ordered conformation, resulting in redshifted, new sharp bands in the absorption and fluorescence spectra. Similar to PIC, the hybrid closely mimics the mechanical properties of biological gels, such as collagen and fibrin, including the strain stiffening properties at low stresses. Moreover, the PMNT/PIC hybrids show much higher ROS production efficiency under red light than PMNT only, leading to an efficient photodynamic antimicrobial effect towards various pathogenic bacteria.
- Published
- 2020
5. Carbon dioxide-controlled assembly based on conjugated polymer and boron nitride
- Author
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Dong Gao, Bo Wang, Liang Qiu, Xue Yang, Yong Zhan, Chengfen Xing, Yang Huang, and Hongbo Yuan
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chemistry.chemical_classification ,Materials science ,02 engineering and technology ,General Chemistry ,Polymer ,Conjugated system ,Fluorene ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Hydrophobic effect ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Boron nitride ,Carbon dioxide ,0210 nano-technology ,Signal amplification - Abstract
CO2-controlled assembly of conjugated polymer and boron nitride (BN) was fabricated via electrostatic and hydrophobic interactions between the BN fiber and conjugated polymer of PFBT containing fluorene units and 2,1,3-benzothiadiazole units. CO2, an effective and green stimulus for regulating the assembly of PFBT and BN fibers, leads to an obvious fluorescence variation. Moreover, PFBT enables the assembly with the signal amplification and light-harvesting properties. This work provides a new triggering method to construct intelligent conjugated polymer-based platform, and offers fluorescence monitoring strategy for carbon dioxide capture.
- Published
- 2020
6. CO2/NIR light dual-controlled nanoparticles for dsDNA unzipping
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Chengfen Xing, Yanjing Wang, Hongbo Yuan, and Dawei Li
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chemistry.chemical_classification ,Nir light ,Materials science ,Biocompatibility ,Photothermal effect ,Nanoparticle ,02 engineering and technology ,General Chemistry ,Polymer ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Biophysics ,0210 nano-technology ,Ethidium bromide ,Electrostatic interaction - Abstract
Both of carbon dioxide (CO2) and near-infrared (NIR) light as triggers for non-invasive remotely control are attracting wide attentions due to their good biocompatibility and easy operation. Here, CO2/NIR light dual controlled nanoparticles are proposed to remotely regulate the unzipping of dsDNA by using imidazole functionalized conjugated polymer nanoparticles (imidazole-CPNs). The dsDNA successfully coats on the shell of imidazole-CPNs to form imidazole-CPNs/dsDNA assembly due to intensively electrostatic interaction triggered by CO2. Furthermore, the unzipping process of dsDNA is remotely controlled by NIR light based on the photothermal effect, and it can be readily monitored by the fluorescence intensity of ethidium bromide (EB) and CD spectra of dsDNA. Thus, dual stimulation responsive imidazole-CPNs effectively control dsDNA unzipping under CO2 stimulus and NIR light, promising a new direction in the biological applications of DNA, such as the treatments of diseases caused by gene duplication abnormality.
- Published
- 2020
7. Synergistic Photodynamic and Photothermal Antibacterial Therapy Based on a Conjugated Polymer Nanoparticle-Doped Hydrogel
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Manman Wu, Qifan Cui, Chengfen Xing, Hongbo Yuan, Gang Ma, and Xueying Bao
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Materials science ,Nir light ,medicine.medical_treatment ,Biochemistry (medical) ,Polymer nanoparticle ,Doping ,technology, industry, and agriculture ,Biomedical Engineering ,Nanoparticle ,Nanotechnology ,Photodynamic therapy ,General Chemistry ,Conjugated system ,Photothermal therapy ,Biomaterials ,White light ,medicine - Abstract
Herein, we have developed a composite antibacterial hydrogel with photodynamic therapy (PDT) and photothermal therapy (PTT) antibacterial capabilities, triggered by white light and NIR light irradiation. A water-insoluble conjugated polymer (PDPP) with photothermal ability was prepared into nanoparticles by the nanoprecipitation method, and the cell-penetrating peptide TAT was grafted on the surface of the nanoparticles. Based on our previous work that developed a hybrid hydrogel with an enhanced PDT effect from polyisocyanide (PIC) hydrogel and cationic conjugated polythiophene (PMNT), PDPP nanoparticles (CPNs-TAT) with photothermal ability are introduced to realize the synergistic antibacterial effect of PDT and PTT. Using the PIC hydrogel to combine PIC and CPNs-TAT has the following advantages. First, the PIC hydrogel can regulate the aggregation state of PMNT, making it better dispersed and improving its capacity of reactive oxygen species (ROS) production. Second, CPNs-TAT can be uniformly dispersed in the PIC hybrid, thereby avoiding the toxicity caused by too high local concentration, achieving a uniform increase in system temperature, and enhancing the therapeutic effect of PTT. Third, the PIC hybrid has the synergistic treatment effect of PDT and PTT. The PIC hybrid intelligently regulates its antibacterial ability through white light and NIR light, which can be used in the white light and NIR light areas. When irradiated with white light and NIR light sequentially, synergistic PDT and PTT exhibit stronger antibacterial ability than PDT or PTT alone. The combination of two antibacterial methods realizes the dual-control antibacterial hydrogel of PDT and PTT and provides an antibacterial mode based on PIC hybrids. Therefore, the PIC hybrids are promising as an antibacterial excipient for clinical wounds.
- Published
- 2022
8. Enhancing the Light Coverage of Photosynthetic Bacteria to Augment Photosynthesis by Conjugated Polymer Nanoparticles
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Dong Gao, Zijuan Wang, Yong Zhan, and Chengfen Xing
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chemistry.chemical_classification ,biology ,Chemistry ,viruses ,Biochemistry (medical) ,Biomedical Engineering ,Nanoparticle ,Peptide ,General Chemistry ,Polymer ,engineering.material ,Conjugated system ,Photosynthesis ,biology.organism_classification ,Biomaterials ,Coating ,Chemical engineering ,engineering ,Photosynthetic bacteria ,Rhodopseudomonas palustris - Abstract
By coating photosynthetic bacteria of Rhodopseudomonas palustris with conjugated polymers nanoparticles modified with positively charged peptide TAT (CPNs-TAT), a bio-optical hybrid composite of R....
- Published
- 2022
9. Near-Infrared Light Regulation of Tumor PI3K/Akt Signaling Pathway for Enhancing Cancer Cell Apoptosis through Conjugated Polymer Nanoparticles
- Author
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Chengfen Xing, Dong Gao, Benkai Bao, Wu Manman, and Ning Li
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Calmodulin ,biology ,Chemistry ,Cell growth ,Biochemistry (medical) ,Biomedical Engineering ,food and beverages ,General Chemistry ,Conjugated system ,Cell biology ,Biomaterials ,Apoptosis ,Calcium-binding protein ,Cancer cell ,biology.protein ,Phosphorylation ,Protein kinase B - Abstract
Calmodulin (CaM), as a calcium binding protein involved in the signal pathways of many life activities such as cell proliferation and apoptosis, can be regulated with the near-infrared (NIR) light-based photothermal conversion. Here, we build a conjugated polymer nanoparticle (CPNs-C) by assembling polypyrrole dione and dipalmitoyl phosphatidylethanolamine-polyethylene glycol-maleimide with a calmodulin antibody modified on the surface, which is NIR light-responsive for photothermally inducing apoptosis of cancer cells. Under near-infrared light irradiation, protein kinase B (Akt) and phosphatidylinositol 3-kinase, which bind to CaM, reduce the degree of phosphorylation due to the photothermal effect of CPNs-C, thus inhibiting the recruitment of Akt on the cell membrane. Therefore, the phosphorylation of GSK-3β downstream of the signaling pathway is reduced, and the phosphorylation of FoxO3a is enhanced, which can promote apoptosis of cancer cells. Compared with the photothermal effect of traditional CPNs, CPNs-C exhibits higher efficiency to regulate signaling pathways to promote cancer cells toward apoptosis. This strategy of utilizing NIR light to regulate the tumor apoptotic signaling pathway provides an effective way to enhance cancer cell apoptosis with high efficiency.
- Published
- 2022
10. Inhibition and disaggregation of amyloid β protein fibrils through conjugated polymer-core thermoresponsive micelles
- Author
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Dong Gao, Hao Geng, Hongbo Yuan, Yongqiang Cheng, Chengfen Xing, and Liang Qiu
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Hot Temperature ,Amyloid β ,Cell Survival ,Polymers ,Biomedical Engineering ,Conjugated system ,Fibril ,Micelle ,PC12 Cells ,Polyethylene Glycols ,Protein Aggregates ,Animals ,General Materials Science ,Cytotoxicity ,Micelles ,chemistry.chemical_classification ,Amyloid beta-Peptides ,Dose-Response Relationship, Drug ,Chemistry ,General Chemistry ,General Medicine ,Polymer ,Peptide Fragments ,Rats ,Toxicity ,Biophysics ,Homeostasis - Abstract
Excess aggregation of amyloid β peptide (Aβ) is a fatal cause of Alzheimer's disease (AD), which leads to physiological toxicity. Inhibiting and disaggregating the Aβ aggregates is an effective strategy to reduce physiological toxicity in neuronal cells. Herein, conjugated polymer-based thermoresponsive micelles (CPMs) were designed with an efficient thermoresponsive surface and a reactive-oxygen-species (ROS)-generating core. In this work, the CPMs exhibited a strong capability to capture the toxic Aβ aggregates at physiological temperature. Under white-light irradiation, ROS was generated in the CPMs, and the toxic Aβ aggregates were efficiently disaggregated through the oxidation of ROS, leading to appropriate Aβ homeostasis between aggregation and disaggregation and reduced the Aβ-induced cytotoxicity. Therefore, the multifunctional micelles of CPMs with both capturing shells and ROS functional cores present a promising strategy to reduce Aβ fibrillation-induced cytotoxicity.
- Published
- 2020
11. Conjugated Polythiophene/Porphyrin Complex for Rapid and Simple Detection of Bacteria in Drinking Water
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Ruimin Niu, Chengfen Xing, Ruihua Li, Wenmin Yan, Hongbo Yuan, Yibing Fan, Gang Li, Yong Zhan, Hailong An, and Junjie Qi
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chemistry.chemical_classification ,Polymers and Plastics ,biology ,Organic Chemistry ,Cationic polymerization ,Polymer ,Conjugated system ,Condensed Matter Physics ,Photochemistry ,biology.organism_classification ,Porphyrin ,chemistry.chemical_compound ,Förster resonance energy transfer ,Adsorption ,chemistry ,Materials Chemistry ,Polythiophene ,Physical and Theoretical Chemistry ,Bacteria - Abstract
An electrostatic complex combining anionic water-soluble conjugated polythiophene (PTP) with commercial cationic porphyrin ([5,10,15,20-tetrakis(4-(trimethylammonio)-phenyl)-21H,23H-porphine tetratosylate] (TMP)) has been designed for rapid and simple detection of bacteria infection in drinking water by applying fluorescence resonance energy transfer (FRET) strategy. There is efficient FRET from PTP to TMP for the electrostatic complex of PTP/TMP. Upon the addition of bacteria, the cationic TMP is adsorbed tightly to the negatively charged membrane surface of bacteria through electrostatic interactions, making the PTP reside in much farther proximity to TMP than that in the absence of bacteria. Therefore, much weaker FRET is observed. Interesting, the detection for bacteria based on FRET changes of PTP/TMP complex is visual in view of the color changes under ultraviolet (UV) irradiation. This assay takes advantage of the light-harvesting property of conjugated polymer and the ratiometric measurements for minimizing the nonspecific binding effect. The strategy is promising in pathogen detection in drinking water and other sources.
- Published
- 2015
12. Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy
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Wenmin Yan, Hongbo Yuan, Chengfen Xing, Hailong An, Yong Zhan, Bing Wang, Shu Wang, Yibing Fan, Ruimin Niu, Ruihua Li, and Junjie Qi
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chemistry.chemical_classification ,animal structures ,Calmodulin ,biology ,Graphene ,Biomolecule ,Conjugated system ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,law.invention ,Biomaterials ,Hydrophobic effect ,Crystallography ,Förster resonance energy transfer ,chemistry ,Phenylene ,law ,Electrochemistry ,biology.protein ,Organic chemistry ,Hybrid material - Abstract
The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell-shaped biomolecule upon Ca2+-binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca2+ than that of CaM in the absence of Ca2+ (apoCaM), resulting in much farther proximity between poly[(9,9-bis(6-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N-terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca2+/CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca2+ ions.
- Published
- 2015
13. A Multiple-Stimulus-Responsive Biomimetic Assembly Based on a Polyisocyanopeptide and Conjugated Polymer
- Author
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Ran Chai, Chengfen Xing, Fanfan Meng, Yong Zhan, Hongbo Yuan, and Yibing Fan
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Polymers ,Supramolecular chemistry ,Polymer architecture ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Supramolecular assembly ,Hydrophobic effect ,Polyfluorene ,chemistry.chemical_compound ,Biomimetic Materials ,Biomimetic synthesis ,Organic chemistry ,Molecular Structure ,Chemistry ,Organic Chemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Biophysics ,0210 nano-technology ,Peptides ,Ethylene glycol ,Hydrophobic and Hydrophilic Interactions - Abstract
An assembly was fabricated and was revealed to be a multiple-stimulus-responsive biomimetic hybrid polymer architecture. It was constructed by the hydrophobic interactions between a conjugated polyfluorene that contained 2,1,3-benzothiadiazole units (PFBT) and a tri(ethylene glycol)-functionalized polyisocyanopeptide (3OEG-PIC). The introduction of PFBT to the polyisocyanopeptide (PIC) network allowed for the incorporation of responsiveness to multiple stimuli including temperature, CO2 , carbonic anhydrase, and nonlinear mechanics, which mimics natural processes and interactions. Furthermore, the light-harvesting and signal amplification characteristics of PFBT endowed the supramolecular assembly with the essential function of fluorescence monitoring for biological processes.
- Published
- 2017
14. Conjugated Polyelectrolyte-Based New Strategy for in Situ Detection of Carbon Dioxide
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Chengfen Xing, Linfei Zhao, Ran Chai, Yong Zhan, Hongbo Yuan, and Yibing Fan
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chemistry.chemical_classification ,In situ ,02 engineering and technology ,Polymer ,Conjugated system ,Fluorene ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrostatics ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Hydrophobic effect ,chemistry.chemical_compound ,chemistry ,Carbon dioxide ,Molecule ,General Materials Science ,0210 nano-technology - Abstract
A conjugated polymer centered on fluorene and 2,1,3-benzothia-diazole (PFBT) is prepared for sensing CO2 in situ with high sensitivity and low background. Upon introducing CO2, the weaker electrostatic repulsion and stronger hydrophobic interactions between neighboring PFBT molecules enhance the interchain contacts compared to that without CO2, leading to the energy transfer from fluorene to 2,1,3-benzothia-diazole sites and the emission color shift from blue to green, which is sensitive to sensing CO2 in atmospheric air with a content of ∼400 ppm. Importantly, PFBT is employed to monitor photosynthesis and respiration upon cycling day and night in situ.
- Published
- 2017
15. Nucleobase-Functionalized Conjugated Polymer for Detection of Copper(II)
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Hongbo Yuan, Yong Zhan, Sichuan Xu, Ruimin Niu, Chengfen Xing, and Hailong An
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Ions ,Steric effects ,Materials science ,Polymers ,Supramolecular chemistry ,Water ,DNA ,Thiophenes ,Conjugated system ,Photochemistry ,Fluorescence ,Phosphates ,Nucleobase ,Electron transfer ,chemistry.chemical_compound ,Spectrometry, Fluorescence ,chemistry ,Side chain ,Polythiophene ,General Materials Science ,Selectivity ,Copper ,Thymidine - Abstract
In recent years, supramolecular organization of thiophene derivatives, oligo- and polythiophene, have been developed with various designs to achieve complex functions. Here, we describe the synthesis and characterization of a conjugated polymer with thymidine side chain bases and polythiophene backbones (PTT) instead of phosphate bonds in DNA, and the PTT exhibits exceptional fluorescence quenching efficiency upon binding of Cu(2+) ions in aqueous medium, which is suggested to be electron transfer from the π* orbit at the excited state of PTT to the 3d orbit of Cu(2+) ions and subsequent Cu(2+)-mediated interpolymer π-stacking aggregation. Furthermore, Cu(2+) ions can be selectively and easily monitored by the fluorescence quenching of PTT, which can be used for detection of Cu(2+) ions with good selectivity and high sensitivity in aqueous medium. Both experimental and theoretical methods have been devoted to demonstrate the strong affinity and steric interaction of PTT toward Cu(2+). These findings will illustrate new directions for the design of nucleobase-functionalized materials with transition metals responsive activity.
- Published
- 2014
16. Design Guidelines For Conjugated Polymers With Light-Activated Anticancer Activity
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Xuli Feng, Libing Liu, Shu Wang, Chengfen Xing, Hongwei Tang, Guillermo C. Bazan, and Qiong Yang
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chemistry.chemical_classification ,Materials science ,Singlet oxygen ,Light activated ,Cancer therapy ,Polymer ,Conjugated system ,Condensed Matter Physics ,Photochemistry ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry.chemical_compound ,chemistry ,Electrochemistry - Published
- 2011
17. Synthesis of Amphiphilic Polythiophene for Cell Imaging and Monitoring the Cellular Distribution of a Cisplatin Anticancer Drug
- Author
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Chengfen Xing, Hongwei Tang, Shu Wang, Qiong Yang, and Libing Liu
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Materials science ,Cell Survival ,Polymers ,Stereochemistry ,Antineoplastic Agents ,Thiophenes ,Conjugated system ,Absorption ,Biomaterials ,Surface-Active Agents ,chemistry.chemical_compound ,Imaging, Three-Dimensional ,Suzuki reaction ,Cell Line, Tumor ,Amphiphile ,medicine ,Side chain ,Humans ,General Materials Science ,Cisplatin ,chemistry.chemical_classification ,Cell Death ,Spectrum Analysis ,Biomolecule ,General Chemistry ,Combinatorial chemistry ,chemistry ,Microscopy, Electron, Scanning ,Polythiophene ,Biotechnology ,medicine.drug ,Conjugate - Abstract
A new amphiphilic conjugated polythiophene derivative (PT-Boc) is synthesized via Suzuki coupling reaction. The amphiphilic characteristic makes it form nanoaggregates in water, in which hydrophobic moieties come together to form the inner core. The polymer shows good photostability and no toxicity to human lung epithelial (A549) cells even at a high concentration (100 μg mL(-1) ). It therefore meets the crucial requirement for cellular imaging and other biological applications. The anticancer drug cisplatin is used as a model, and is linked to polythiophene to obtain a conjugate 'PT-Pt' by coordinated interactions between cisplatin and the amine groups of the polythiophene side chain. The PT-Pt can be used for monitoring the cellular distribution of cisplatin by fluorescence microscopy. The amphiphilic polythiophene provides a platform for fluorescent imaging of drugs and biological molecules in living cells.
- Published
- 2011
18. Conjugated Polymer Nanoparticles for Drug Delivery and Imaging
- Author
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Qiong Yang, Fengting Lv, Xuli Feng, Libing Liu, Shu Wang, Chengfen Xing, and Hongwei Tang
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Lung Neoplasms ,Materials science ,Polymers ,Glutamic Acid ,Nanoparticle ,Quantum yield ,Nanotechnology ,In Vitro Techniques ,Conjugated system ,Fluorescence ,Drug Delivery Systems ,Cell Line, Tumor ,polycyclic compounds ,Humans ,General Materials Science ,Cytotoxicity ,chemistry.chemical_classification ,Fluorenes ,Antibiotics, Antineoplastic ,technology, industry, and agriculture ,Cationic polymerization ,Polymer ,Combinatorial chemistry ,carbohydrates (lipids) ,chemistry ,Doxorubicin ,Drug delivery ,Nanoparticles - Abstract
We prepared a new conjugated polymer nanoparticle with the size of about 50 nm that is prepared by electrostatic assembly of cationic conjugated polymer PFO and anionic poly(l-glutamic acid) conjugated with anticancer drug doxorubicin (PFO/PG-Dox). The PFO exhibits good fluorescence quantum yield, photostability, and little cytotoxicity to meet the essential requests for cell imaging. In PFO/PG-Dox nanoparticles, the fluorescence of PFO is highly quenched by Dox by electron transfer mechanism, and thus the PFO is in the fluorescence "turn-off' state. After PFO/PG-Dox nanoparticles are exposed to carboxypeptidase or are taken up by cancer cells, the poly(l-glutamic acid) is hydrolysed to release the Dox, inducing the activation of PFO fluorescence to "turn-on" state. This multifunctional nanoparticle system can deliver Dox to targeted cancer cells and monitor the Dox release based on fluorescence "turn-on" signal of PFO, which concurrently images the cancer cells. The present work opens the door for new functional studies of conjugated polymer in simultaneous imaging and disease therapeutics.
- Published
- 2010
19. Synthesis of Zwitterionic Water-Soluble Oligofluorenes with Good Light-Harvesting Ability
- Author
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Chengfen Xing, Zhiqiang Shi, Shu Wang, Libing Liu, and Yuliang Li
- Subjects
chemistry.chemical_classification ,Carboxylic acid ,Intermolecular force ,Photodissociation ,Polymer ,Conjugated system ,Condensed Matter Physics ,Photochemistry ,Fluorescence ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry.chemical_compound ,Förster resonance energy transfer ,chemistry ,Electrochemistry ,Fluorescein - Abstract
A new water-soluble zwitterionic oligofluorene bearing carboxylic acid and quaternary ammonium as pendant groups (OF-1) is synthesized and characterized. It forms aggregates by intermolecular electrostatic interactions and exhibits similar light-harvesting ability as that of conjugated polymers. Efficient fluorescence resonance energy transfer (FRET) occurs from OF-1 to double-stranded DNA tagged with fluorescein (dsDNA-F1). A photoresponsive oligofluorene (OF-3) is also synthesized by protecting OF-1 with 1-(2-nitrophenyl)ethanol. Photolysis of OF-3 can produce OF-1 to result in a fluorescence "turn-on" response, thus the FRET from OF-3 to dsDNA-FI can be turned on by light irradiation. OF-3 offers the potential for remote DNA sensing.
- Published
- 2010
20. Gadolinium(III) chelated conjugated polymer as a potential MRI contrast agent
- Author
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Chengfen Xing, Lingling An, Shu Wang, Minghui Yu, Litao Zhu, Qingling Xu, and Fude Feng
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Polymers and Plastics ,Biocompatibility ,Gadolinium ,MRI contrast agent ,Organic Chemistry ,Spin–lattice relaxation ,chemistry.chemical_element ,Conjugated system ,Nuclear magnetic resonance ,chemistry ,Suzuki reaction ,Polymer chemistry ,Materials Chemistry ,Side chain ,Chelation - Abstract
Nuclear magnetic resonance imaging (MRI) has become a powerful technique in clinical diagnostics. In this work, a new MRI contrast agent by covalently linking Gd(III) chelates to the side chain of conjugated polymer (PF–Gd) is synthesized by Suzuki cross-coupling reaction. The PF–Gd exhibits a higher relaxivity and a pronounced enhancement in contrast than that of (NMG) 2 –Gd–DTPA widely used for clinical diagnosis. This work should be feasible to potentially lead to a new class of imaging contrast agents.
- Published
- 2010
21. Conjugated Polymer-Based Nanoparticles for Cancer Cell-Targeted and Image-Guided Photodynamic Therapy
- Author
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Chunmei Yan, Yong Zhan, Ran Chai, Yulan Chen, Fanfan Meng, Junjie Qi, Liang Qiu, Chengfen Xing, and Fei Peng
- Subjects
chemistry.chemical_classification ,Polymers and Plastics ,Chemistry ,medicine.medical_treatment ,Organic Chemistry ,Nanoparticle ,Nanotechnology ,Photodynamic therapy ,02 engineering and technology ,Polymer ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,Folic acid ,Polymer chemistry ,Cancer cell ,Materials Chemistry ,medicine ,Physical and Theoretical Chemistry ,0210 nano-technology - Published
- 2017
22. Fluorescence Turn-On Detection of Nitric Oxide in Aqueous Solution Using Cationic Conjugated Polyelectrolytes
- Author
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Zhiqiang Shi, Chengfen Xing, Minghui Yu, Daoben Zhu, Yuliang Li, and Shu Wang
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Aqueous solution ,Polymers and Plastics ,Organic Chemistry ,Inorganic chemistry ,Cationic polymerization ,Conjugated system ,Photochemistry ,Fluorescence ,Conjugated Polyelectrolytes ,Photoinduced electron transfer ,Polyelectrolyte ,chemistry.chemical_compound ,chemistry ,Materials Chemistry ,Imidazole - Abstract
A fluorescent turn-on detection for nitric oxide in aqueous solution is developed using cationic conjugated polymers. The assay benefits from the sensitivity of optical signals from conjugated polymers and the simplicity of fluorescence measurement techniques. The assay contains three elements: a cationic conjugated polymer that contains imidazole moieties, Cu 2+ ions, and the target nitric oxide. The highly fluorescent conjugated polymer coordinates to Cu 2+ ions through weak N . . . Cu interactions, and its fluorescence is efficiently quenched by a photoinduced electron transfer process ('off' state). In the presence of nitric oxide, the transformation of the paramagnetic Cu 2+ ion into a diamagnetic Cu 1+ ion inhibits the quenching and, therefore, the fluorescence of the conjugate polymer is recovered ('on' state). Other biologically relevant reactive nitrogen species, such as NOBF 4 , NaNO 2 , and NaNO 3 don't exhibit the fluorescence recovery of the conjugated polymer under the same conditions as nitric oxide. The cationic conjugated polymer/Cu 2+ complex can thus be used as a platform to detect nitric oxide in aqueous solution with high sensitivity and selectivity.
- Published
- 2007
23. Conjugated Polythiophene for Rapid, Simple, and High-Throughput Screening of Antimicrobial Photosensitizers
- Author
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Ruimin Niu, Chengfen Xing, Huanrong Li, Hailong An, Hongbo Yuan, Junjie Qi, Wenmin Yan, Yibing Fan, Yong Zhan, and Ruihua Li
- Subjects
Materials science ,Light ,Cell Survival ,Polymers ,High-throughput screening ,Drug Evaluation, Preclinical ,Cell Separation ,Thiophenes ,Conjugated system ,Photochemistry ,Hydrophobic effect ,chemistry.chemical_compound ,Anti-Infective Agents ,Escherichia coli ,General Materials Science ,Photosensitizer ,Photosensitizing Agents ,Cationic polymerization ,Equipment Design ,Fluorescence ,High-Throughput Screening Assays ,Equipment Failure Analysis ,stomatognathic diseases ,Treatment Outcome ,chemistry ,Polythiophene ,Biosensor - Abstract
The cationic conjugated poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrochloride] (PMNT) has been developed for high-throughput screening of photodynamic antimicrobial chemotherapy photosensitizers (PSs). The bacterial number can be detected quantitatively by PMNT via various fluorescence quenching efficiencies. The photosensitized inactivation of bacteria is not efficient with ineffective PSs, and thus the bacteria grow exponentially and can be coated tightly by PMNT through electrostatic and hydrophobic interactions, resulting in aggregates and fluorescence quenching of PMNT, whereas, conversely, effective PSs lead to original and strong fluorescence of PMNT. This new platform of high-throughput screening is promising for discovering new PSs.
- Published
- 2015
24. Carbon Dioxide-Controlled Assembly of Water-Soluble Conjugated Polymers Catalyzed by Carbonic Anhydrase
- Author
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Ruimin Niu, Chengfen Xing, Hongbo Yuan, Ran Chai, Fei Peng, Yong Zhan, Yibing Fan, and Junjie Qi
- Subjects
Polymers and Plastics ,Polymers ,Surface Properties ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,Carbonic Anhydrase II ,01 natural sciences ,Catalysis ,Hydrophobic effect ,Carbonic anhydrase ,Materials Chemistry ,Molecule ,Organic chemistry ,Particle Size ,chemistry.chemical_classification ,Aqueous solution ,biology ,Chemistry ,Organic Chemistry ,Water ,Polymer ,Carbon Dioxide ,021001 nanoscience & nanotechnology ,Fluorescence ,Combinatorial chemistry ,0104 chemical sciences ,Solubility ,Biocatalysis ,biology.protein ,0210 nano-technology - Abstract
The CO2 -responsive and biocatalytic assembly based on conjugated polymers has been demonstrated by combining the signal amplification property of the polythiophene derivative (PTP) and the catalytic actions of carbonic anhydrase (CA). CO2 is applied as a new trigger mode to construct the smart assembly by controlling the electrostatic and hydrophobic interactions between the PTP molecules in aqueous solution, leading to the visible fluorescence changes. Importantly, the assembly transformation of PTP can be specifically and highly accelerated by CA based on the efficient catalytic activity of CA for the inter-conversion between CO2 and HCO3- , mimicking the CO2 -associated biological processes that occurred naturally in living organisms. Moreover, the PTP-based assembly can be applied for biomimetic CO2 sequestration with fluorescence monitoring in the presence of CA and calcium.
- Published
- 2017
25. Ca2+-controlled assembly for visualized detection of conformation changes of calmodulin
- Author
-
Yong Zhan, Hailong An, Ruimin Niu, Hongbo Yuan, Chengfen Xing, Ruihua Li, and Wenmin Yan
- Subjects
Calmodulin ,Light ,Stereochemistry ,Polymers ,Protein Conformation ,viruses ,Static Electricity ,Target peptide ,Thiophenes ,Conjugated system ,Polythiophene derivative ,Scattering, Radiation ,General Materials Science ,skin and connective tissue diseases ,biology ,Aqueous medium ,Chemistry ,Electrostatics ,Spectrometry, Fluorescence ,Color changes ,biology.protein ,Biophysics ,Calcium ,Spectrophotometry, Ultraviolet ,sense organs ,Peptides ,Protein Binding - Abstract
A new strategy has been designed for visualized detection of the conformation changes of calmodulin bound to target peptide (CaM-M13) based on the conformation sensitive property of a water-soluble conjugated polythiophene derivative (PMNT) and the electrostatic interactions of PMNT/CaM-M13. Interestingly, the direct visualized PMNT color changes under UV irradiation and the turbidity changes of samples in aqueous medium can be applied to detect the conformation changes as well as the controllable assembly of PMNT/CaM-M13 with Ca(2+) in aqueous medium. Because of the specific binding of Ca(2+), the assembly of PMNT/CaM-M13 can be applied to sense calcium as well.
- Published
- 2014
26. Conjugated polymers for light-activated antifungal activity
- Author
-
Gaomai Yang, Fengting Lv, Chengfen Xing, Qiong Yang, Shu Wang, and Libing Liu
- Subjects
animal structures ,Antifungal Agents ,Porphyrins ,Light ,Polymers ,Microbial Sensitivity Tests ,Thiophenes ,Conjugated system ,Photochemistry ,environment and public health ,Biomaterials ,chemistry.chemical_compound ,polycyclic compounds ,General Materials Science ,Irradiation ,chemistry.chemical_classification ,Singlet oxygen ,Cationic polymerization ,General Chemistry ,Polymer ,Porphyrin ,Photoexcitation ,enzymes and coenzymes (carbohydrates) ,chemistry ,Energy Transfer ,Polythiophene ,Aspergillus niger ,Biotechnology - Abstract
A cationic polythiophene-porphyrin (PTP) dyad is shown to exhibit efficient light-activated antifungal activity. Higher singlet oxygen (¹O₂) generation efficiency can be attained from PTP upon photoexcitation due to the light-harvesting properties of the polymer backbone and efficient energy transfer from the polythiophene to the porphyrin units. PTP can be used for treating fungal infections in lower doses of irradiation light and polymer concentration.
- Published
- 2011
27. Conjugated polymer/porphyrin complexes for efficient energy transfer and improving light-activated antibacterial activity
- Author
-
Hongwei Tang, Shu Wang, Libing Liu, Chengfen Xing, and Qingling Xu
- Subjects
Microbial Viability ,Porphyrins ,Light ,Singlet oxygen ,Polymers ,Cationic polymerization ,General Chemistry ,Microbial Sensitivity Tests ,Thiophenes ,Conjugated system ,Photochemistry ,Biochemistry ,Porphyrin ,Catalysis ,Anti-Bacterial Agents ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Membrane ,Intersystem crossing ,chemistry ,Energy Transfer ,Escherichia coli ,Molecule ,Polythiophene ,Bacillus subtilis - Abstract
With the increasing antibiotic resistance of microorganisms, there is a growing interest in the design and development of new materials that are effective in killing bacteria to replace conventional antibiotics. Herein, a new anionic water-soluble polythiophene (PTP) and a cationic porphyrin (TPPN) are synthesized and characterized. They can form a complex through electrostatic interactions, and efficient energy transfer from PTP to TPPN occurs upon irradiation under white light (400-800 nm). The energy of TPPN transfers to triplet by intersystem crossing, followed by sensitization of oxygen molecule to enhance the efficiency of singlet oxygen generation related to TPPN itself. The positive charges of PTP/TPPN complex promote its adsorption to the negatively charged bacteria membranes of gram-negative Escherichia coli and gram-positive Bacillus subtilis through electrostatic interactions, and the singlet oxygen effectively kills the bacteria. The photosensitized inactivation of bacteria for the PTP/TPPN complex is efficient, and about 70% reduction of bacterial viability is observed after only 5 min of irradiation with white light at a fluence rate of 90 mW x cm(-2) (27 J x cm(-2)). The technique provides a promising application in photodynamic inactivation of bacteria on the basis of enhanced energy transfer offered by light-harvesting conjugated polymers.
- Published
- 2009
28. Conformation Changes: Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy (Adv. Funct. Mater. 28/2015)
- Author
-
Shu Wang, Bing Wang, Yibing Fan, Hongbo Yuan, Ruimin Niu, Junjie Qi, Wenmin Yan, Chengfen Xing, Hailong An, Yong Zhan, and Ruihua Li
- Subjects
chemistry.chemical_classification ,Materials science ,Calmodulin ,biology ,Graphene ,Oxide ,Nanotechnology ,Polymer ,Conjugated system ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,law.invention ,Biomaterials ,chemistry.chemical_compound ,Förster resonance energy transfer ,chemistry ,law ,Polymer chemistry ,Electrochemistry ,biology.protein ,Hybrid material - Published
- 2015
29. Antifungal Activity: Conjugated Polymers for Light-Activated Antifungal Activity (Small 4/2012)
- Author
-
Shu Wang, Chengfen Xing, Libing Liu, Fengting Lv, Gaomai Yang, and Qiong Yang
- Subjects
chemistry.chemical_classification ,Antifungal ,Singlet oxygen ,medicine.drug_class ,Light activated ,General Chemistry ,Polymer ,Conjugated system ,Biomaterials ,chemistry.chemical_compound ,chemistry ,medicine ,Organic chemistry ,General Materials Science ,Biotechnology - Published
- 2012
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