1. Applications of imaging flow cytometry in the diagnostic assessment of acute leukaemia.
- Author
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Grimwade LF, Fuller KA, and Erber WN
- Subjects
- Aneuploidy, Automation, Laboratory, Chromosomes, Human, Pair 15 metabolism, Chromosomes, Human, Pair 17 metabolism, Flow Cytometry instrumentation, Gene Expression, Humans, Image Cytometry instrumentation, In Situ Hybridization, Fluorescence methods, Interphase, Leukemia, Promyelocytic, Acute genetics, Leukemia, Promyelocytic, Acute pathology, Mutation, Nuclear Proteins genetics, Nuclear Proteins metabolism, Nucleophosmin, Oncogene Proteins, Fusion genetics, Oncogene Proteins, Fusion metabolism, Phenotype, Chromosomes, Human, Pair 15 ultrastructure, Chromosomes, Human, Pair 17 ultrastructure, Flow Cytometry methods, Image Cytometry methods, Leukemia, Promyelocytic, Acute diagnostic imaging, Translocation, Genetic
- Abstract
Automated imaging flow cytometry integrates flow cytometry with digital microscopy to produce high-resolution digital imaging with quantitative analysis. This enables cell identification based on morphology (cell size, shape), antigen expression, quantification of fluorescence signal intensity and localisation of detected signals (i.e. surface, cytoplasm, nuclear). We describe applications of imaging flow cytometry for the diagnostic assessment of acute leukaemia. These bone marrow malignancies are traditionally diagnosed and classified by cell morphology, phenotype and cytogenetic abnormalities. Traditionally morphology is assessed by light microscopy, phenotyping by conventional flow cytometry and genetics by karyotype and fluorescence in situ hybridisation (FISH) on interphase nuclei/metaphase spreads of cells on slides. Imaging flow cytometry adds a new dimension to the diagnostic assessment of these neoplasms. We describe three specific applications: From this we conclude that imaging flow cytometry offers benefits over conventional diagnostic methods. Specifically the ability to visualise the cells of interest, the pattern and localisation of expressed antigens and assess cytogenetic abnormalities in one integrated automated high-throughput test. Imaging flow cytometry presents a new paradigm for the diagnostic assessment of leukaemia., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2017
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