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[Detection of t(15;17) using cytogenetic, fluorescent in situ hybridization, and molecular techniques. Comparative study of 11 patients with acute promyelocytic leukemia].
- Source :
-
Sangre [Sangre (Barc)] 1997 Feb; Vol. 42 (1), pp. 11-5. - Publication Year :
- 1997
-
Abstract
- Objectives: Analysis and comparison of the sensitivity for the detection of t(15; 17) with conventional cytogenetic (CC), Southern blotting (SB), metaphase fluorescence in situ hybridization (M-FISH), interphase fluorescence in situ hybridization (I-FISH) and polymerase chain reaction (PCR) techniques.<br />Patients and Methods: 11 acute promyelocytic leukaemia (APL) patients (9 M3 and 2 M3v) were studied at diagnosis and partial remission and analyzed with CC (11 cases), SB (7 cases), I-FISH (2 cases) and PCR (3 cases) techniques.<br />Results: The percentages of detection of t(15;17) were: 90.9% (CC), 71.4% (SB), 85.7% (M-FISH) and 100% (I-FISH and PCR). Additional chromosomal abnormalities were detected in 2 cases with CC techniques.<br />Conclusions: 1.- There is a very good correlation in the detection of t(15; 17) between the techniques analyzed. 2.- At diagnosis, partial remission and relapse, M-FISH and PCR provide a reliable diagnosis of t(15; 17). Nevertheless, it is important to perform a CC analysis because it enables the recognition of additional chromosome abnormalities with putative prognostic importance. 3.- At complete remission, I-FISH and PCR provide a rapid and safe screening of putative residual cells. 4.- The great sensibility of the techniques available for the detection of t(15; 17) translocation allows the use in each laboratory of the most appropriate techniques according to their substructure and the patients' clinical stage.
- Subjects :
- Chromosomes, Human, Pair 15 genetics
Chromosomes, Human, Pair 17 genetics
DNA, Neoplasm genetics
Female
Humans
Leukemia, Promyelocytic, Acute mortality
Leukemia, Promyelocytic, Acute pathology
Leukemia, Promyelocytic, Acute therapy
Male
Neoplasm Proteins analysis
Neoplasm Proteins genetics
Oncogene Proteins, Fusion analysis
Oncogene Proteins, Fusion genetics
Prognosis
Remission Induction
Sensitivity and Specificity
Chromosomes, Human, Pair 15 ultrastructure
Chromosomes, Human, Pair 17 ultrastructure
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Promyelocytic, Acute genetics
Polymerase Chain Reaction
Translocation, Genetic
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0036-4355
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Sangre
- Publication Type :
- Academic Journal
- Accession number :
- 9229797