1. Mechanical characterization of base analogue modified nucleic acids by force spectroscopy†
- Author
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L. Marcus Wilhelmsson, Yii-Lih Lin, Felix Ritort, Uttama Dutta, Fredrik Westerlund, Xavier Viader-Godoy, and Vinoth Sundar Rajan
- Subjects
Models, Molecular ,Stacking ,General Physics and Astronomy ,010402 general chemistry ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Models ,Fluorescence Resonance Energy Transfer ,Transition Temperature ,Physical and Theoretical Chemistry ,Base (exponentiation) ,030304 developmental biology ,Fluorescent Dyes ,Mechanical Phenomena ,0303 health sciences ,Chemistry ,Intermolecular force ,Force spectroscopy ,Molecular ,DNA ,Combinatorial chemistry ,Single Molecule Imaging ,3. Good health ,0104 chemical sciences ,Characterization (materials science) ,Nucleic acid ,Nucleic Acid Conformation ,Thermodynamics - Abstract
We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy., Investigating the forces in nucleic acids using single base-pair level modifications and optical tweezers.
- Published
- 2021