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Low Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms
- Source :
- Pandit, S, Ravikumar, V, Abdel-Haleem, A M, Derouiche, A, Mokkapati, V R S S, Sihlbom, C, Mineta, K, Gojobori, T, Gao, X, Westerlund, F & Mijakovic, I 2017, ' Low Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms ', Frontiers in Microbiology, vol. 8, 2599 . https://doi.org/10.3389/fmicb.2017.02599, Frontiers in Microbiology, Frontiers in Microbiology, Vol 8 (2017)
- Publication Year :
- 2017
-
Abstract
- Extracellular polymeric substances (EPS) produced by bacteria form a matrix supporting the complex three-dimensional architecture of biofilms. This EPS matrix is primarily composed of polysaccharides, proteins and extracellular DNA. In addition to supporting the community structure, the EPS matrix protects bacterial biofilms from the environment. Specifically, it shields the bacterial cells inside the biofilm, by preventing antimicrobial agents from getting in contact with them, thereby reducing their killing effect. New strategies for disrupting the formation of the EPS matrix can therefore lead to a more efficient use of existing antimicrobials. Here we examined the mechanism of the known effect of vitamin C (sodium ascorbate) on enhancing the activity of various antibacterial agents. Our quantitative proteomics analysis shows that non-lethal concentrations of vitamin C inhibit bacterial quorum sensing and other regulatory mechanisms underpinning biofilm development. As a result, the EPS biosynthesis in reduced, and especially the polysaccharide component of the matrix is depleted. Once the EPS content is reduced beyond a critical point, bacterial cells get fully exposed to the medium. At this stage, the cells are more susceptible to killing, either by vitamin C-induced oxidative stress as reported here, or by other antimicrobials or treatments.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Sodium ascorbate
quantitative proteomics
030106 microbiology
lcsh:QR1-502
vitamin C
Bacillus subtilis
Polysaccharide
Microbiology
lcsh:Microbiology
exopolymeric matrix
03 medical and health sciences
chemistry.chemical_compound
Extracellular polymeric substance
Extracellular
Quantitative proteomics
Exopolymeric matrix
Vitamin C
Original Research
chemistry.chemical_classification
biology
Chemistry
Biofilm
biology.organism_classification
Quorum sensing
Biochemistry
Biofilms
biofilms
Bacteria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Pandit, S, Ravikumar, V, Abdel-Haleem, A M, Derouiche, A, Mokkapati, V R S S, Sihlbom, C, Mineta, K, Gojobori, T, Gao, X, Westerlund, F & Mijakovic, I 2017, ' Low Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms ', Frontiers in Microbiology, vol. 8, 2599 . https://doi.org/10.3389/fmicb.2017.02599, Frontiers in Microbiology, Frontiers in Microbiology, Vol 8 (2017)
- Accession number :
- edsair.doi.dedup.....cac038a5cb469208cbc8ca749218ef4d