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Teucrium polium has been used in traditional medicine as antifungal, antipyretic, antispasmodic, and antibacterial. It is consumed by many jordanians for the treatment of many diseases. The effects of this plant have been investigated in kidney, liver, and brain. Its antidiabetic, antimicrobial, antioxidant, and anticancer effects have been introduced. Polyphenolic compound, flavonoids, monoterpenes, alkanoides, and essential oils were identified. Several studies revealed that this plant has a hypoglycemic effect and can help to control blood sugar. It was reported that plants containing flavonoids and phenolics compounds exhibit a large array of biological activities like genotoxicity (chromosomal aberrations and sister chromatid exchange) and oxidative stress damage. These phytochemicals are found in herbal and vegetables plants, as well as being reliably protective against oxidative stress damage and lipid peroxidation. In addition, T. polium has secondary effects on different organs, namely liver, kidney and at high doses this plant becomes toxic. In conclusion, this review investigates many pharmacologicals properties and side effects of T. polium.

Objective: This study was conducted to investigate the effects of adding oregano (Origanum syriacum L.) and germander (Teucrium polium L.) to poultry diets individually and/or in combination: i) on cooked chicken meat quality and storage stability, ii) to compare this effect with those of the synthetic antioxidant butylated hydroxyanisole (BHA) and with the normal basic diet (Control: without supplements).Methods: Broilers (140 birds) were raised for 21 days and then equally divided into five different treatment groups of 28 birds each. The dietary treatments were as follows: i) control; ii) germander (GER, 1.5%); iii) oregano (ORE, 2.5%); iv) combination of GER and ORE (CM, 1.5%, and 2.5%); v) BHA (0.02%). Meat patties from the five treatments were prepared, cooked, and stored at 4°C prior to analysis. During storage, samples were measured for thiobarbituric acid-reactive substances (TBARS) and total carbonyl levels at 0, 4, and 7 days. In addition, cooked thigh meat was prepared separately to evaluate cooking loss and sensory attributes.Results: The CM dietary treatment showed the highest antioxidant effect, with decreasing TBARS values (breast and thigh meat) throughout the storage time (4 to 7 days). Furthermore, ORE showed a higher antioxidant effect, decreasing the rancidity development (TBARS values), compared to the GER during the storage period (days 0 to 7). The anticarbonyl effect of the CM supplement was the highest among all treatments from day 0 to 7. Generally, the antioxidant effect of GER was lower compared to that of ORE and BHA alone. The CM treatment most significantly decreased off-odor and rancidity development, with the lowest oxidation odor scores.Conclusion: The results indicate that the combination of oregano and germander in the diet of boilers improves meat quality and prolongs shelf life.

Objective: Angiogenesis plays a dominant role in many pathophysiologic disorders, including cancer. Tranilast, which is an anti-fibrotic drug, is also suggested as an anti-angiogenesis agent. As Teucrium polium (TP) is known as an herbal medicine with antitumor properties, this study aimed to investigate the effects of TP and Tranilast on human umbilical vein endothelial cells (HUVECs), in vitro model of angiogenesis, as well as rat’s aortic ring ex vivo model. Methods: In this study, The HUVECs were treated with various doses of TP and Tranilast each one alone or in combination together. Cell survival test, aortic ring ex-vivo assay, and evaluating mRNA expressions of VEGFA and TGF-β ligands and receptors were performed. Results: The survival rate of HUVECs has significantly (p

The emergence of antibiotic-resistant bacterial strains leads to the need to find new antibacterial agents. Due to their properties, silver nanoparticles and plant extracts are more often considered as promising antibacterial agents. The aim of this work was to study the antibacterial properties of silver nanoparticles and evaluate the potential effects of the combination of silver nanoparticles and Teucrium polium extract. In this study, we investigated the antibacterial activity of silver nanoparticles and a 96% ethanol extract of T. polium against wild-type strains of E. coli K-12 DSM 1116 and S. aureus MDC 5233. The results showed that the plant extract and silver nanoparticles have antibacterial effects. The T. polium 70% ethanol extract had no antibacterial effect, but provided an increase in the antibacterial activity of silver nanoparticles (by 4.5 times against E. coli K-12 and by 2 times against S. aureus). The 96% ethanol extract made it possible to increase the antibacterial activity of silver nanoparticles against E. coli K-12 by 4.2 times and S. aureus by 6.6 times. Thus, the synergistic antibacterial effect between the extract and nanoparticles, which might be due to different mechanisms of action of the constituents, was observed.

Pseudomonas aeruginosa is a common pathogen in a wide range of infections, from urinary tract infections to bacteremia. Intrinsic resistance to antimicrobial agents in this bacterium worsens the condition of the treatment of infections caused by it. The aim of this study was to investigate the antimicrobial effects of ethanol, methanol, and ethyl acetate Teucrium polium Extract and Citrullus colocynthis on a p. aeruginosa isolated from Zabol hospital. P. aeruginosa specimens were collected from Zabol city. Plant extracts were prepared using a rotary machine and the minimum inhibitory concentration and minimum trace concentration were determined by microdilution method. The lowest inhibitory concentration of methanolic extract of C. colocynthis against p. aeruginosa was 1.25 mg / ml while the lowest inhibitory concentration of ethyl acetate extract of C. colocynthis was 0.62 mg / ml, two strains were inhibited in this concentration and the highest inhibitory concentration was 5 mg, 6 strains of bacteria in this concentration has been restrained. The results of this study showed that watermelon extract of T. polium Extract and C. colocynthis have a significant antibacterial effect. Extract from this plant can be used to deal with specific pathogenic bacteria.

Teucrium polium leaf is one of the folkloric medicinal plants used traditionally to treat many diseases in the Palestinian community. It has antibacterial antioxidant and anti-inflammatory consequences. Present study aims the evaluating the in vitro inhibitory effect of Teucrium polium leaf extracts on tumor necrosis factor-α (TNF-α) using polymorphonuclear cells (PMNCs), in addition to determine its antioxidant and total phenolic and flavonoids contents. Polymorphonuclear cells were withdrawn from whole blood according to Histopaque (Ficol-1077) method. Blood cells were cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. The levels of tumor necrosis factor (TNF-α) were determined after 24 h using LPS stimulation. Total phenolic contents, flavonoids contents and antioxidant activity were measured using spectrophotometric method. The TNF-α concentrations were compared using paired-samples t test. The leaf extracts of Teucrium polium revealed significant reduction in terms of TNF-α levels. The extract contained high phenolic and flavonoids contents and its antioxidant activities were remarkable. The reduced values in the TNF-α levels as affected by Teucrium polium leaf extracts indicate its effect in anti-inflammation. The plant is rich with polyphenolic compounds and flavonoids and has strong antioxidant activity. The observed anti-inflammatory effect of the extracts under study may be discussed as the influence of the significant presence of the phenolic compounds and flavonoids.

The wound healing properties of Teucrium polium L., a plant used in the Algerian traditional medicine for the treatment of wounds, have been investigated using an excision wound model in rabbits. An ointment was prepared with two concentrations (5 and 10%) of a methanolic extract of the aerial parts. Both preparations showed significant effect on the wound contraction when compared to the control and the group treated with petroleum jelly. In addition, acute dermal and oral toxicity was assessed in animal models. The absence of signs of toxicity on the skin of rabbits indicated the safety of the ointment. After oral administration in mice at doses of 1000 and 2000 mg/kg b.wt, no signs of liver and kidney toxicity were detected by analysis of biochemical parameters and by histological examination. The composition of the methanolic extract was investigated by HPLC-PDA-MS analysis, and a comprehensive profile of phenolic compounds was established, with 14 flavonoids and phenylethanoid glycosides identified as the main polyphenolic constituents. Overall, the data support the use of T. polium as a wound healing agent in the Algerian traditional medicine.

Identification of phenolic compounds, antioxidant activity, and their potential applications in pharmaceutical and medical aspects has received much attention due to their benefits for human health...

This study aimed to formulate sodium lauryl sulfate cross-linked chitosan beads and sodium alginate-chitosan films for designing a dressing that would shorten the healing time of skin wounds. Teucrium polium extract-loaded chitosan-sodium lauryl sulfate beads (CH-SLS) and chitosan-alginate (CH-ALG) films were prepared and characterized by using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). The swelling properties of the CH-SLS beads were also analyzed in a water solution. The obtained Teucrium polium extract-loaded CH-SLS beads and CH-ALG films (TBF) were further incorporated into the commercial adhesive dressing. This TBF wound dressing was then investigated for evaluation of its wound healing potential in the mice using the excision wound model. Healing was assessed by the macroscopic appearance and the rate of wound contraction during 8 days. On day 4, the TBF-treated wounds exhibited 98% reduction in the wound area when they were compared with healing ointment, elastic adhesive dressing, and untreated wounds which were exhibited 63%, 43%, and 32%, respectively. Furthermore, the application of TBF dressing reduced skin wound rank scores and increased the percentage of wounds contraction. These results demonstrate that TBF dressing improves considerably the healing rate and the macroscopic wound appearance at a short delay and this application may have therapeutic benefits in wound healing.

The utilization of natural materials in the post-harvest process of agricultural products is necessary for the production of safe food. In recent years, the use of essential oil extracted from aromatic plants has shown significant potential for insect pest management. Toxicity and antifeedant effects of essential oil isolated from aerial parts of Teucrium polium L. have been investigated against the red flour beetle, Tribolium castaneum Herbst, as one of the most detrimental insect pests of post-harvest cereals in the present study. The chemical profile of this oil was also assessed by gas chromatography-mass spectrometry (GC-MS) and lycopersene (26.00%), dodecane (14.78%), 1,5-dimethyl decahydronaphthalene (9.27%) and undecane (7.18%) were identified as main components. For evaluation of the fumigant toxicity and antifeedant effects using multiple regression analysis, a quadratic polynomial and linear equation were obtained, respectively. Adequacy and accuracy of the fitted models were checked through analysis of variance. T. polium essential oil exhibited significant fumigant toxicity on the T. castaneum adults and based on modeling using RSM, the concentration of 20 µl/l and 72 min exposure time was calculated as the optimum conditions for 97.97% mortality with 87.8% desirability. A concentration of 14.13 µl/l was also estimated as the optimum value for 94.66% Feeding Deferens Index with 92% desirability. The mortality and anti-nutritional effect, in general, increased with increasing of exposure times and the essential oil concentrations. Results designated a great potential of T. polium essential oil for management of the red flour beetle. Further, it was found that the Response Surface Methodology was a promising method for the prediction of these bio-effects.

Medicinal plants provides natural product may be used as precursors for new drug for their chemical diversity. In the present study, the potential of gamma radiation as sterilizing agent for preservation and its effect on phytochemical contents, antioxidant and antibacterial activity of Teucrium polium (T. polium) was analyzed for antimicrobial and antitumor activity. The results revealed that exposing plant powder to doses of gamma irradiation (5,10,15,20 KGy) significantly increased the ethyl acetate extraction yield (dry weight) as compared to the non-irradiated plant (control). Teucrium polium phytochemical contents of total phenolic content (TPC), total flavonoids content (TFC) and tannins content were significantly increased with gamma irradiation. In the same line, plant extract antioxidant activity was significantly increased in response manner to radiation dose. Teucrium polium ethyl acetate extracts shown antibacterial activity against Staphylococcus aureus and Pseudomonas aeroginosa. Gamma radiation doses caused significant increase in the antibacterial activity against P. aeroginosa and S. aureus at dose of 20 KGy. Total count of microbial load of T. polium was significantly reduced at 5.0 kGy by 99.95% with total decontamination of fungi count.

Background: Several metabolic pathways are involved in the complications of diabetes like polyol pathway. Aldose reductase (AR) is a key enzyme in the polyol pathway, which catalyzes the conversion of glucose to sorbitol. AR inhibitors are appropriate to prevent and treat the diabetes complications. Objective: This study was designed to investigate the effect of different fractions of Teucrium polium on the AR activity. Methods: Fifty cow’s eye lenses were prepared and AR enzyme was purified according to the Hyman-Kinoshita method. The enzyme activity in the presence of the crude extract and different fractions of Teucrium polium (1, 5, 10, 20 and 100 μg/ml) was measured. In addition, IC50 content of fractions was also measured for the neutralization of DPPH free radical. Since some AR inhibitors are phenolic compounds, the phenolic and flavonoid contents have been investigated. Results: Results showed that the highest phenol and flavonoid content and the lowest IC50 value (3.67 µg/ml) for AR inhibition were related to the ethyl acetate fraction. Line weaver-Burk plot showed that ethyl acetate fraction acts as a non-competitive enzyme inhibition. Conclusion: Thus, T. polium can be proposed as a therapy to prevent or treat chronic complications of diabetes in the future.

The aim of the present study was to determine the chemical composition and antioxidant activity of essential oils (EOs) from two Teucrium polium subspecies, to evaluate, also their antibacterial activities, against some nosocomial-bacteria. The phytochemical screening of essential oils was analyzed using gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry analysis (GC-MS). The antibacterial activities were assessed by disc diffusion method and minimal inhibitory concentration (MIC), against Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Citrobacter koseri and Acinetobacter baumannii) and Gram-positive bacteria Staphylococcus aureus. The antioxidant potential was evaluated in vitro by three assays, namely free radical scavenging activity against 1,1-diphenyl-2-picrylhydrzyl (DPPH), ferric reducing activity power (FRAP) and total antioxidant capacity. Twenty-six components were identified in the EO of Teucrium polium subsp. aurum representing. Its major component was Caryophyllene (19.13%) followed by γ-Muurolene (13.02%), τ-cadinol, (11.01%), α-Gurjunene (9.2%), Rosifoliol (8.79%), 3-Carene (7.04%). However, twenty two components were identified in the EO of T. polium subsp. polium. Its major components are 3-carene (16.49%), γ-Muurolene (14.03%), α-pinene (9.94%), α-phellandrene (6.93%) and Caryophyllene (7.51%). The antibacterial activity of both essential oils showed a higher activity against tested nosocomial bacteria especially against S. aureus and A. baumannii. The EO of T. polium subsp. aureum showed better antioxidant activity as measured by DPPH and FRAP assays with IC50 values of 3.7 ± 0.2 mg/ml and 2.31 ± 0.11 mg/ml, respectively. The total antioxidant capacity assay showed that T. polium subsp. aureum had a significant activity with value to 3308.27 mg equivalent to ascorbic acid/g of EO. The Moroccan T. polium essential oils could be exploited as an antimicrobial agent for the treatment of several infectious diseases caused by bacteria, especially, those who have developed resistance to conventional antibiotics. Keywords: Teucrium polium, GC-MS, Nosocomial infections, Antibacterial, Antioxidant

Green synthesis of silver nanoparticles (AgNPs) using a number of biological agents such as extracts from various parts of the plants have garnered remarkable attention recently. The aim of the study was a green synthesis of AgNPs using stems and flowers of Felty germander (Teucrium polium L.). After collection and preparation of the aqueous extract, production of AgNPs was performed. In the study, effect of parameters including AgNO3 concentration (1, 5, 10, 15, 20, 25, and 50 mM), aqueous extract (100 µl, 150 µl, 300 µl, 600 µl and 900 µl), pH value (between 0 and 14), incubation time (5, 10, 15, 20, 25, and 30 min), and temperature (30 °C, 60 °C, and 90 °C) were investigated in the synthesis of AgNPs. The sufficient production was achieved at the room temperature (30 °C), duration of 15 min, pH of 6 and concentration of 7.5 mM of AgNO3. Finally, the antifungal activity of these nanoparticles was also studied on Fusarium oxysporum by colony formation assay. The production of AgNPs was identified by an absorption peak at approximately 450 nm using UV–Visible spectroscopy. According to XRD, DSL, FESEM, and PSA results and analysis of SEM, nanoparticles have almost spherical shape and size of 10 to 100 nm. The SEM images also revealed that the prepared AgNPs are particles with no agglomeration. Furthermore, the TEM images exhibited that the AgNPs were well dispersed and there was no evident aggregation. Absorption peaks at 3391.55, 2917.00, 2848.83, 1640.15, 1384.65, 1243.37, 1069.07 and 614.54 cm−1 were observed for the AgNPs using FTIR analysis. On the other hand, the antifungal analysis revealed that these AgNPs have antifungal activity. For the first time, the biosynthesis of AgNPs was successfully performed using the aqueous extract of Felty germander which is a rapid, inexpensive and safe approach. Furthermore, the resulting AgNPs has antifungal activity.

In this study, antioxidant activity and protective effects of Teucrium polium extract in stabilizing of canola oil during frying were tested and compared to synthetic antioxidant, BHA. Total phenolic, α‐tocopherol, flavonoid, and condensed tannin content of Teucrium polium extracted by ethanol ultrasound‐assisted were 60.90 mg/g, 103.66 μg/ml, 4.36 mg/g, and 3.77 mg/g, respectively. Moreover, IC50 of the extract was 924.21 ppm. Canola oil samples containing 200, 600, and 1,000 ppm of the extract were heated at 180˚C for 30 hr and compared with BHA. Progress of oil oxidation was determined by measuring their peroxide value (PV), acid value (AV), iodine value (IV), carbonyl value (CV), color index (CI), conjugated diene value, and total polar compounds (TPC). The results showed that the extract was capable of retarding oil oxidation and deterioration significantly (p, Canola oil formulated with Teucrium polium extract showed the lower degradation of oil oxidation products during frying. Therefore, Teucrium polium extract can be used as natural antioxidant to stability and safety of canola oil during frying.

Introduction Phytochemicals are one wide class of nutraceuticals found in plants which act as antioxidants. In this research, the essential oil (EO) of Teucrium polium L., Lamiaceae, collected from Mascara province, situated in the Algerian northwestern, where their chemical composition varies according to geographical origin, season variation, and climatic conditions were studied. Materials and Methods The extraction of EO was performed by hydrodistillation. Then, the chemical compounds were identified by gaz chromatography coupled to a mass spectrometer (GC–MS). In parallel, the antioxidant activity was evaluated using the DPPH test. Results The yield of the EO of T. polium L. varied during different seasons with the highest in winter season, at vegetative stage (S1), while the same EO (S1) was significantly more efficient as an antioxidant than the EO harvasted at the flowering stage (S2) with IC50 values 3.90±0.05, 16.14±0.15 mg/mL, respectively (P < 0.001). These extracts are predominantly constituted by limonene (29.87%-26.39%), spathulenol (17.24%-13.29%), camphor (0.0%-8.20%), pinocarvone (7.76%-5.60%), tau-cadinol (5.41%-3.67%), pinene oxide (0.0%-4.78%), α-terpineol (0.0%-4.6%), 1-adamantanemethylamine (0.0%-9.80%) and β- myrcene (0.0%-4.02%). Conclusions The results show that both EOs can be considered as potential sources of natural antioxidants. However, the vegetative stage was the best stage for harvesting the EO of T. polium L. which can be used as an alternative source of synthetic compounds.

Background: The aqueous extract of some plants has an antibacterial effect on pathogenic bacteria for humans. Objective: This study designed to investigate the antimicrobial activity of aqueous extract of some plants on some food-borne pathogenic bacteria in a laboratory setting has done. Method: In an experimental study, plants collected from of Tehran city and aqueous extract of them, extracted. To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with two ways of visual and turbidity (OD), serially different concentrations (1.25-80 mg/ml) on different cultures was prepared. Disc diffusion (DD) test in concentrations of 10, 20 and 40 mg/ml to determine the average diameter of inhibition for some bactria, done. Results: The results showed that the inhibitory and fungicidal aqueous extract of Solanum americanum on various bacteria was more than any other aqueous extracts (Staphylococcus aureus MIC = 10 mg/ml) and also Staphylococcus aureus and Salmonella typhimurium were the most sensitive and most resistant bacteria to aqueous extract, respectively. Conclusion: According to the results, we can be hopeful the extract of Solanum americanum in the treatment of pathogenic bacteria such as S. aureus, can be help and it can used in the food industry as a food protection. Recommended by extracting the active ingredients of the extract of this plant and other plants, more research done on the composition of extract.

Teucrium polium extract (TPE) is a natural product with potent anticancer activity because of its terpenoid and flavonoid content. The aim of this study was to synthesize solid lipid nanoparticles (SLN) containing T. polium extract (TPE-SLNs) and to evaluate its anti-cancer effect. Formulations of TPE-SLNs were prepared using high-shear homogenization followed by the ultra-sonication technique. Then the TPE-SLNs were characterized by dynamic light scattering (DLS), Zetasizer and field-emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy methods. After confirming the presence of nanoparticles, its anti-proliferative activity was evaluated on Ntra-2 cancer cells and compared to human foreskin fibroblasts (HFF) as a normal cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The pro-apoptotic (flow cytometry) and quantitative PCR (qPCR) and anti-angiogenic (chick chorioallantoic membrane (CAM)) and anti-inflammatory (qPCR) effects of TPE-SLNs were assessed by various methods. TPE-SLNs (85.5 nm, PDI: 0.39, and zeta potential: -25.5 mv) were inhibited by the proliferation of Ntra-2 cancer cells with half-maximal inhibitory concentration (IC50 ; 106.58 µg/ml). Moreover, by increasing the expression of caspase-3,9 and decreasing the expression of interleukin 6 (IL-6), IL-1b, and Bcl-2 genes, apoptosis and anti-inflammatory effects were observed. Reduction of angiogenesis was observed by the reduction of genes involved in angiogenesis and reduction of vascular and embryonic factors in the CAM assay. Activation of various mechanisms of inhibiting cancer cells in the treatment of nanoparticles was demonstrated in the present study. Therefore, these nanoparticles can be recommended for therapeutic purposes. PRACTICAL APPLICATIONS: Solid lipid nanoparticles (SLNs) are composed of pure solid fats and have been researched for many applications. Advantages such as high safety, low toxicity, control of drug release, and increase in chemical stability of loaded drugs distinguish these systems from other colloidal systems. According to the results of this study, solid lipid nanoparticles (SLN) containing T. polium extract showed their anti-cancer effects through various strategies such as inhibiting angiogenesis, inhibiting inflammation, and inducing apoptosis on Ntra-2 cells. These results make it possible to apply these nanocarriers in the loading and transport of drugs to treat various diseases such as cancer.

Objectives Medicinal plants continue to gain popularity on a global scale. Besides, there is a need for discovering new antibacterial natural extracts that could be used as antibiotics alternatives against resistant bacteria. In this respect, the aim of this study was to determine the phytochemicals, antioxidant activity, calorific nutritional value and antibacterial potential of four traditionally used wild medicinal plants (Achillea fragrantissima (Delile) Hayne, Teucrium polium L., Peganum harmala L. and Solenostemma argel (Forssk.) Sch. Bip. grow in Saint Catherine Protectorate, South Sinai, Egypt. Methods Standard methods were applied to determine the proximate composition, calorific nutritional value, secondary metabolites (phenolics and flavonoids), antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH·), ferrous ion chelating (FIC), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS +) and Ferric reducing antioxidant power (FRAP) techniques in addition to the antibacterial potential of the active aqueous extracts by broth dilution technique. Results The results obtained illustrated that T. polium recorded the highest nutritional calorific value (205.63 ± 5.76 calories/100 g dried plant), total phenolics (8.057 ± 0.322 g gallic acid equivalent/100 g dried plant), total flavonoids (2.013 ± 0.034 g catechin equivalent/ 100 g dried plant and antioxidant scavenging activity using DPPH (EC50 = 1.84 mg extract/g DPPH), FIC (IC50 = 0.068 mg extract/ml), ABTS (61.11%) and FRAP (2185.71 mmol Fe (II)/g extract) assays followed descendingly by A. fragrantissima, S. argel and P. harmala, respectively. There was direct relation between the flavonoids/ phenolics ratios and the radical scavenging activity among all extracts. Regarding the antibacterial potential, the extracts expressed broader antibacterial spectrum against Bacillus cereus (ATCC®11778™), Bacillus subtilis (ATCC®19659™), Escherichia coli (ATCC®10536™), klebsiella pneumoniae (ATCC®10031™), Listeria innocua (ATCC®33090™), Listeria monocytogenes (ATCC®19115™), Pseudomonas aeruginosa (ATCC®9027™), salmonella enterica (ATCC®15479™) and Salmonella typhimurium)ATCC®14028™(strains. The minimal inhibitory concentrations of the extracts estimated using broth dilution assay ranged from 0.049 to 1.56 mg/ml. T. polium extract possessed the highest activity among all other extracts. Conclusions In conclusion, the studied medicinal plants could be used as nutritional supplements, antioxidants and antibacterial botanicals for combating some of the pathogenic bacteria.

Diabetic foot ulcer is one of the major complications among diabetic patients. Several studies have shown that the extract of Teucrium polium (T. polium) is effective in the treatment of diabetic and non-diabetic wounds, as well as burn wounds. The aim of current study was to assess the wound healing activity of T. polium extract ointment in diabetic rats. Sixty-four male Wistar rats were induced diabetes with alloxan injection (125 mg/kg) and surgical wound induced. The rats were divided into 8 groups of eight rats each: control group, eucerin group, phenytoin group, 2%, 3%, 4%, 5% and 10% T. polium groups. The ointment was dressed on the wound twice a day. The process of wound healing was screened by macroscopy and digitalization on days 14 and 21 and until complete wound healing. There was no infection in the wounds of rats in any groups. The process of wounds healing of the 2%, 3%, 4%, 5%, and 10% T. polium ointments, with phenytoin ointment and base ointment (eucerin) on day 14 and 21, showed that the significant difference between the treatment groups with 4% (P = 0.003), 5% (P = 0.001), 10% (P = 0.001) T. polium ointment and phenytoin ointment group (P = 0.001) compared to eucerin group. The results of this study showed that T. polium extract ointment with a 10% ointment accelerates the wound healing process in diabetic rats and is comparable to the phenytoin group.

The plant Teucrium polium (T.p.) possesses a wide range of pharmacological activities due to the presence, in particular, of different phytochemicals, phenols and flavonoids. We examined the effects of the T.p. extract on impulse activity of hippocampal neurons in rats with a model of Alzheimer’s disease. Adult albino rats were divided into three groups (5 animals in each). The control group C obtained intracerebroventricular (i.c.v.) infusions of saline, animals of group Aβ were i.c.v. infused with amyloid β peptide 25–35, Aβ(25–35), and group Aβ+T.p. was treated by both Aβ(25–35) infusions and introductions of the T.p. extract. In group Aβ, a greater proportion of hippocampal neurons with post-tetanic depression after high-frequency stimulation of the entorhinal cortex, lower values of the frequency of background activity generated by the above neurons, and relatively weaker modifications of spike activity (post-tetanic potentiation and depression) were observed. Daily administrations of the T.p. extract partially but considerably reversed the above-mentioned negative shifts in spike activity of hippocampal neurons caused by Aβ(25–35). We suggest that T.p. extract containing important phytochemicals is capable of ameliorating the memory dysfunction caused by Aβ(25–35) via blocking amyloid deposition and a positive influence on the functions of hippocampal neurons.

Green synthesis of silver nanoparticles using the aqueous extract of Ferula gumosa, Ferula latisecta, Teucrium polium and Trachomitum venetum leaves and stems extract as the reducing and stabilizing agents. This synthesis shows attractive characteristics such as; the use of inexpensive and available plant extracts, non-toxicity, eco-friendly biological materials, and operational simplicity. The extracts incubated with AgNO3 solution showed gradual change in color of the extracts to yellowish brown, with intensity increasing during the period of incubation. The nanoparticles were characterized by UV–visible absorption spectroscopy, X-ray diffraction (XRD) spectroscopy, Dynamic Light Scattering (DLS), and Transmission Electron Microscope (TEM). The silver nanoparticles synthesized were generally found to be spherical in shape with variable size ranging from 5 to 30 nm, as evident by TEM. The biosynthesized silver nanoparticles (AgNPs) showed good antibacterial activity against clinical strains of two bacteria Staphylococcus aureus and Escherichia coli.

This study investigated the effect of Teucrium polium L. essential oil (Tp EO) and α-pinene as its major component on the detoxifying- and intermediary-engaged enzymes of Ephestia kuehniella Zeller. Fourth instar larvae were separately fed on the treated artificial diets incorporated with LC50 concentration of Tp EO (4.91 μl ml-1) and α-pinene (10.66 μl ml-1). After three time intervals (24, 48, 72 hours), larval hemolymph was extracted to determine enzymatic activities. Activity of esterase, alanine and aspartate aminotransferases, aldolase, lactate dehydrogenase, and alkaline phosphatase were decreased in the treatments compared with the control. No significant differences were observed between some enzymes in the treated larvae highlighting their ineffectively role to detoxify and to metabolize Tp EO and α-pinene in the hemolyph of E. kuehniella larvae.

Teucrium polium ( T. polium ) and Rumex crispus ( R. crispus ) are plant species that grow widely in Anatolia and are thought to have healing effects for many diseases. In this study plant extracts are suggested as alternative agents in repairing cellular damage by using sister chromatid exchange (SCE), micronucleus (MN), mitotic index (MI), replication index (RI) and nuclear abnormalities (NAs), against the genotoxicity of cyclophosphamide (CP) in the human lymphocyte cells. 8 experimental groups were formed in the study. The cell culture medium was supplemented with 0.16 μg/ml CP and the cells were treated with 50, 100 and 250 μM T. polium and R. crispus extracts in the presence and absence of CP. As a result, CP significantly decreased MI frequency while increasing SCE, MN and NAs frequencies in cells. 100 μM T. polium plus CP decreased SCEs when compared with CP alone. In addition, MN frequency was significantly decreased in 100 μM T. polium plus CP and 250 μM R. crispus plus CP combine groups. Our results suggest that these plant extracts are not genetically damaging and have improving effects at these doses.

Four neo-clerodane diterpenoids, the new polivincins A – C and the known teulamifin B, were isolated by the phytochemical investigation of the acetone extract prepared from the aerial parts of Teucrium polium subsp. vincentinum, (Lamiaceae). The structure and stereochemistry of the compounds were established by IR, HRMS and different ( Al-Khalil, 1995 ) NMR and 2D-NMR techniques.

Bioactive compounds, antioxidant activity and extraction yield of Teucrium polium aerial parts by different concentrations of aqueous and alcoholic solvents [water, ethanol and ethanol:water (50:50)] were investigated by ultrasonic and maceration. The total phenolic, tocopherol, tannin, flavonoid content and antioxidant activity (200–1200 ppm) of the extracts were determined and compared with butylated hydroxyanisol (BHA) (as a synthetic antioxidant) by DPPH· assay, β-carotene/linoleic bleaching inhibition and OSI methods. The results showed that the highest extraction yield (40.26%) and total phenolic (65.74 mg/g), tocopherol (112.13 µg/mL), tannin (5.90 mg/g) and flavonoid (5.60 mg/g) content were observed in ethanol:water (50:50) ultrasonic extraction. Moreover, as the highest bioactive components extracted by ethanol:water (50:50) ultrasonic methods, it signified that the highest radical scavenging activity with 91.45% and β-carotene/linoleic bleaching 84.14% inhibition at 1200 ppm of the extract which is higher than BHA (200 ppm) with 88.31% DPPH· radical scavenging activity and 76.83% inhibition in β-carotene/linoleic bleaching assay. Furthermore, addition of water as the safe, available and inexpensive solvent increased efficiency of ethanol.

The objective of the present study was to determine antioxidant activities, total phenolic and total flavonoid contents of methanol, ethanol, water and ethyl acetate extracts of Moroccan Germander (Teucrium polium). Total phenolic and flavonoid contents were determined spectrophotometrically using gallic acid and rutin as standards. Antioxidant activities was evaluated in vitro by three assays namely free radical scavenging activity against 1,1-diphenyl-2-picrylhydrzyl (DPPH), ferric reducing antioxidant power (FRAP) and total antioxidant capacity. The total phenolic and flavonoid contents were significantly higher in the methanolic extract (P

A novel adsorbent prepared from the leaf powder of Teucrium polium L. (TPLLP) was characterized and its ability for adsorption of Cong red (CR) was inspected. Influences of CR concentration, adsorbent dosage, time of agitation, pH of solution, and temperature on the performance of this adsorption were also examined. Three models of kinetic along with three different isotherm models were applied for analyzing the empirical data of this adsorption. Additionally, the thermodynamic constants of were decided. The surface area, pore volume, pore size, and pHZPC of Zn/Cu-TPLLP were found to be 2.6436 m2.g-1, 0.013317 cm3.g-1, 527.393 Å, and 8.8, respectively. The achieved outcomes indicate the positive influence of temperature, concentration of CR in the range of 20 to 900 mg/L, adsorbent mass in the range of 0.005 to 0.02 g, time of adsorption from 0 to 120 min, and pH from 5.5 to 8.5. Models of the 2nd order with R 2 ≥ 0.995 and Langmuir with R 2 ≥ 0.990 were the best among the other kinetic and isotherm models applied in this research. Moreover, superior capacities of 526.32, 666.67, and 909.09 (mg.g-1) were stemmed at 27, 42, and 57°C, respectively. The outcomes of the thermodynamic evidenced that this adsorption is spontaneous and a heat absorber.

In this study, we investigate the phytochemical profile, anticancer, and antioxidant activities of Teucrium polium methanolic extract using both in vitro and in silico approaches. The results showed the identification of 29 phytochemical compounds belonging to 13 classes of compounds and 20 tripeptides using High Resolution-Liquid Chromatography Mass Spectrometry (HR-LCMS). 13R-hydroxy-9E,11Z octadecadienoic acid, dihydrosamidin, valtratum, and cepharantine were the main compounds identified. The tested extract showed promising antioxidant activities (ABTS-IC50 = 0.042 mg/mL, 1,1-diphenyl-2-picrylhydrazyl (DPPH)-IC50 = 0.087 mg/mL, &beta, carotene-IC50 = 0.101 mg/mL and FRAP-IC50 = 0.292 mg/mL). Using both malignant Walker 256/B and MatLyLu cell lines, T. polium methanolic extract showed a dose/time-dependent antitumor activity. The molecular docking approach revealed that most of the identified molecules were specifically binding with human peroxiredoxin 5, human androgen, and human progesterone receptors with high binding affinity scores. The obtained results confirmed that T. polium is a rich source of bioactive molecules with antioxidant and antitumor potential.

The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) and Herpes simplex virus type 2 (HSV-2) were used for the antiviral activities. Chromobacterium violaceum (ATCC 12472 and CV026) and Pseudomonas aeruginosa PAO1 were used as starter strains for the anti-quorum sensing tests. Isoprenoids are the main class of compounds identified, and 13R-hydroxy-9E,11Z-octadecadienoic acid, valtratum, rhoifolin, sericetin diacetate, and dihydrosamidin were the dominant phytoconstituents. The highest mean diameter of growth inhibition zone was recorded for Acinetobacter baumannii (19.33 &plusmn, 1.15 mm). The minimal inhibitory concentrations were ranging from 6.25 to 25 mg/mL for bacterial strains, and from 6.25 to 25 mg/mL for Candida species. The 50% cytotoxic concentration on VERO (African Green Monkey Kidney) cell lines was estimated at 209 &micro, g/mL. No antiviral activity was recorded. Additionally, T. polium extract was able to inhibit P. aeruginosa PAO1 motility in a concentration-dependent manner. However, the tested extract was able to inhibit 23.66% of the swarming and 35.25% of swimming capacities of PAO1 at 100 &micro, g/mL. These results highlighted the role of germander as a potent antimicrobial agent that can interfere with the virulence factors controlled by the quorum-sensing systems.

Objectives: Regarding the high prevalence of cancer in Iran and the cytotoxic properties of T. polium, the current study aimed to investigate the cytotoxic and anti-mutagenicity effect of T. polium essential oil (TpEO) on human colon adenocarcinoma cell line (HT29). Methods: HT29 cells were cultured in L-glutamine, RPMI Sigma (1640), with 10% of FBS (fetal bovine serum). Then, the cultures were incubated with 5% CO2 at 37°C, and MTT assay was used to recognize cell proliferation under the inhibitory influence of TpEO. The cell cycle progression was monitored by Sub-G1 apoptosis assay using flow cytometry. Eventually, the anti-mutagenicity property was evaluated using the Ames test employing TA100 and exposure to sodium azide as the carcinogenic substance. Results: The cytotoxic effect of TpEO on HT29 cells was 66.867 ± 1.37 µg/mL. Cultured HT29 cells treated with TpEO exhibited morphological features of apoptosis. TpEO preventive effect was about 78.18%. Conclusions: This study showed that TpEO may be useful for treating colon cancer.

Essential oils (EOs) have been described as promising eco-friendly secondary products of aromatic plants with several biological activities. The present study aimed to characterize the chemical composition and explore phytotoxic and antimicrobial activities of Teucrium polium and Thymus decussatus EOs extracted using hydrodistillation (HD) and microwave-assisted extraction (MAE) methods. Twenty-seven and twenty-eight compounds were identified from HD and MAE extracted EOs of T. polium, respectively. The oxygenated sesquiterpenes (57.68%) were characterized as the main components of the hydrodistilled EO with a prominence of 6-epi-shyobunol (33.00%), while sesquiterpene hydrocarbons (54.48%) were the main components of the MAE method, with a prominence of delta-cadinene (25.13%). Eighteen and nineteen compounds, were characterized in T. decussatus EOs extracted using HD and MAE methods, respectively, and oxygenated monoterpenes represented the main components of both EOs with carvacrol (94.40% and 75.91%, respectively) as the main compound. The EOs extracted using the MAE method were slightly more phytotoxic than those extracted using the HD method. The T. decussatus EO extracted using the MAE method showed a higher inhibitory effect than T. polium by 16-, 32-, and 24-fold, regarding seed germination, shoot, and root growth of lettuce, respectively. Moreover, EOs extracted by HD method showed a similar pattern with 16-, 28-, and 14-fold effects. Both T. decussatus EOs exhibited potent inhibitory effect against all tested bacteria with an inhibition zone of 34&ndash, 39 mm and the lowest minimum inhibitory concentration (MIC) of 0.49, 0.98, and 1.95 &mu, g/mL against Aspergillus niger, Escherichia coli, and Staphylococcus aureus, respectively. However, the EOs of T. polium showed weak antibacterial activity and no antifungal effect. Further studies are needed for the characterization of bioactive major compounds, either singular or synergistic, at field scale and to determine their modes of action and safety.

Teucrium polium has been used to treat gastrointestinal disorders, inflammation, diabetes and rheumatism. This plant has beneficial therapeutic properties. In this research the changes in the composition of essential oils from the aerial parts of Teucrium polium were determined at different temperatures and times. The essential oil of air-dried samples was obtained by hydro distillation and analyzed by gas chromatography/mass spectrometry (GC/MS). Changes in essential oils compositions were detected during storage for 3 months in refrigerator, freezer, and at room temperature. The results indicated that at room temperature, the proportions of the compounds with lower boiling temperatures such as Cis-Verbenol (2.11-1.78%) and Germacrene D (1.56-0.94%) were decreased significantly. Furthermore, the essential oil composition showed the least alterations and Teucrium polium L. kept its primary quality when stored at low temperatures, particularly in refrigerator and freezer (Except: α-Pinene, β-Pinene and Limonene).

The Mediterranean flora is characterized by an abundance of aromatic plants. These plants make a significant contribution to the Mediterranean ecological communities both in terms of species numbers and biomass. In this area, Teucrium is a polymorphic and cosmopolitan genus of perennial plants, the largest of the Lamiaceae family, which comprises more than 300 species, of which almost 50 are native to Europe. The members of this genus are commonly known as germanders: they are bitter, astringent and antirheumatic herbs, utilized as antispasmodic agents for gastric ulcer and intestinal inflammation, as diuretic, antiseptic, antipyretic and antihelmintic agents. Moreover, they were also used in food preparation. Teucrium species are rich in essential oils and the presence of various biologically active monoterpenoid and sesquiterpenoid with several biological activities has been reported. This review summarizes the chemical features of the essential oils of Teucrium species reported in literature: it provides an overview on their compositions with particular reference to the more abundant components and to the reported biological activities. Teucrium polium, T. chamaedrys, T. flavum and T. capitatum are the most studied taxa concerning the volatile oil composition. Moreover, it is possible to distinguish different types of essential oils, characterized by the prevalence of a specific class of compounds: in particular, we recognized volatile oils rich in caryophyllene and caryophyllene derivatives, α- and β-pinene, germacrene B and D, α-, γ-, δ-cadinene and/or cadinol, limonene, and bisabolol/bisabolene. This richness in chemodiversity confers to the essential oils from Teucrium species distinctive biological properties and applications, often confirming the traditional and folk uses of this genus. This review testifies the high great variability of Teucrium essential oils and shows that the possible factors responsible for the chemical polymorphism of essential oils and consequently for their bioactivity need further investigation.

Teucrium species have been used in traditional medicine for the treatment of different diseases and for this reason, it is very important to know their effect on the human genome. In this chapter are presented literature data of genotoxic potential of secondary metabolites (phenolic acids and flavonoids) and different extracts from Teucrium species using different valid methods for the detection of genotoxicity. The presented results of investigating the genotoxic effect of methanolic extracts from Teucrium chamaedrys, T. montanum, T. scordium, T. arduini and T. flavum, methanolic, ethanolic, and aqueous extracts from Teucrium polium, as well as methanolic, ethanolic and chloroform extracts from Teucrium ramosissimum using micronucleus assay in human peripheral blood lymphocytes (PBLs), Comet assay in PBLs, Ames test/Salmonella typhimurium. It is known that the biological activities of the herbal extracts associated with its phytochemical composition. Phytochemical analyses of examined extracts of Teucrium species showed that they were rich in polyphenolic compounds and could be considered responsible for antimutagenic properties of the plants. Presented results have shown that the investigated of the Teucrium species possess the protective properties against antineoplastic drug-induced genetic damage in a different type of cells. There are two possible genoprotective effects, antimutagenic/antioxidative (Teucrium polium and T. ramosissimum) and proapoptotic (Teucrium chamaedrys, T. montanum, T. scordium, T. arduini and T. flavum). The investigated Teucrium species showed a genotoxic effect in high concentrations, while in combination with known mutagenic in the same concentrations had a protective effect. Presented investigations from different genotoxicity studies have showed that secondary metabolites have a genoprotective effect on different types of cells (PBLs, mice bone marrow cells, PCEs; Chinese hamster ovary cells, CHO; bacteria Salmonella typhimurium). Moreover, present results clearly showed that tested extracts Teucrium species had a protective effect on the genotoxicity of different agents (mitomycin C, MMC; N-methyl-N-nitro-N-nitrosoguanidine, MNNG; benzo[a] pyrene, B[a]P; aflatoxin B1, AFB1; hydrogen peroxide, H2O2) in a different type of cells in vitro and, therefore, they can be a potential source of new pharmaceutics that will have a significant protective effect on induced genetic damage by chemotherapeutics in healthy cells.

It was approved that flavonoids (luteoline, apigenin) and phenylpropanoid glycosides verbascoside best separation was implemented by using UM detector and isocratic isolation regime. Depending on the growing conditions main influencing substance quantities fluctuate: in the wild growing plants were synthesized nearly 2 times more than in hydroponic growing plants. For the study were obtained 500 alcoholic extracts from hydroponic and wild growing T. polium. The studied samples before analysis were developed three-chloral-acetic-acid anhydride and centrifuged by 10 minutes 12000 rev/m speeds. The solution volume of each injected analysis was 10 mkl. Then some amount of standard verbascoside, luteolin and apigenin samples were solved in 1ml ethyl alcohol and were filled into the special test tubes for analysis. By T. polium chloroform-methanol and tower chromatography water fractions teupolizoid, verbascoside and poliumozid phenylpropanoid glycosides were separated which Rf were equal to 0.25, 0.5, 0.37 in our researches in the past. These compounds composition was proved by complex spectroscopic methods. They had close structure to each other and verbascoside enclosed peaks in HPLC were supposed that belong to poliumozid and teupoliozid and also was brought the above mentioned compounds quantities by calculating chromatography data. Keywords: Teucrium polium L., wild and hydroponic, HPLC, standardization, verbascoside, apigenine, luteolin.

In this work, we studied the essential oils (EOs) obtained by hydrodistillation and lipophilic fractions extrated by carbon dioxide supercritical fluid extraction (SFE-CO2) from Teucrium polium (Lamiaceae). EOs and SFE-CO2 extracts were analyzed by GC and GC-TOFMS. EOs showed qualitative differences compared with SFE-CO2 extracts, though in both products germacrene D was the most abundant component. Notably a new EO chemotype, characterzed by germacrene D, β-eudesmol, shyobunol, and δ-cadinene, was reported. EOs along with SFE-CO2 extracts, plant powder, and solid residue after SFE were evaluated for total phenolic content (TPC) and antioxdiant activity by DPPH, FRAP, and ORAC assays. The raw plant material was the most active, followed by the solid residue after SFE-CO2 extraction. On the other hand, EO and SFE-CO2 extracts displayed weak activity. These results showed that the antioxidant compounds of T. polium are of hydrophilic nature and are not extracted or retained in the plant material after SFE-CO2 extraction.

BACKGROUND: Teucrium polium L. aerial parts are traditionally used in Algerian folk medicine due to its therapeutic properties. OBJECTIVE: The present study was designed to evaluate the effect of different extracting solvents (methanol, ethanol, ethyl acetate, chloroform, hexane and water) on antioxidant capacity and total phenol and flavonoid contents of Algerian Teucrium polium aerial parts. METHODS: The antioxidant capacity of Teucrium polium extracts was assessed by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) free radicals scavenging and reducing power assays. For each extract, the total phenol and flavonoid contents were investigated by the Folin-Ciocalteu and aluminium trichloride methods, respectively. RESULTS: All the tested extracts showed an appreciable total phenolic and total flavonoid contents and strong antioxidant capacity. Among the different extracts, the methanolic extract was found to be containing the highest amount of total phenols (206.95±1.82 mg of gallic acid equivalent per gram of extract) and flavonoids (42.16±0.61 mg of quercetin equivalent per gram of extract). The same extract also exhibited a much better reducing power and scavenging capacity against DPPH (IC50 = 6.77±0.15 mg/l) and ABTS (IC50 = 2.79±0.07 mg/l). CONCLUSION: These findings suggest that aerial parts of Teucrium polium methanolic extract could be used as natural source of antioxidants in food, pharmaceutical and cosmetic industry.

INTRODUCTION: Teucrium polium is a herbal plant which has curative potential and pharmacological characteristics. Therefore, establishment of accurate analytical methods for characterising its active components is of prime importance. OBJECTIVE: Because of the complex nature of essential oil samples, some results of direct gas chromatography–mass spectrometry (GC–MS) analysis maybe unreliable. Therefore, GC–MS is coupled with chemometric methods to resolve experimental information taken from chromatographic peaks, and assign compounds to them more correctly. METHODS: Essential oil of T. Polium of Koohbanan suburb (Kerman, Iran) was extracted by hydro‐distillation. Then the T. polium essential oil was qualitatively analysed using GC–MS coupled with multivariate curve resolution (MCR), to resolve experimental information taken from chromatographic peaks, and assign compounds to them more correctly. After that, the method of parallel factor analysis (PARAFAC) was implemented to quantify the concentrations of five major components of the T. polium essential oil. RESULTS: The number of identified components increases from 74 using the direct method of analysis to 106 using the MCR method. It is shown that α‐pinene (8.93 μg/L), cis‐verbenol (3.98 μg/L) and myrtenal (1.02 μg/L) are the most abundant components in the T. polium essential oil. CONCLUSION: By coupling GC–MS and chemometric resolution methods such as MCR‐alternating least squares (ALS) and PARAFAC more information with higher precision and accuracy can be obtained from raw experimental data. Also, it is shown that by implementing the PARAFAC method on the GC–MS data, it is possible to measure the concentration of the constituents of co‐eluted, overlapped or embedded chromatographic peaks.

This study was conducted to evaluate the phytochemical analysis, and in vitro anticancer screening of four wild plants grown in the northern region of the Kingdom of Saudi Arabia (KSA), namely: Convolvulus oxyphyllus, Rhazya stricta, Astragalus kahircus and Teucrium polium. Total phenolics content, flavonoids, anthocyanins, saponins, total antioxidant capacity (TAC) and 2,2-diphenyl-1picrylhydrazyl (DPPH) scavenging activity were assessed in their extracts. In vitro anticancer screening of the hydro-alcohol extracts was also assessed using human hepatocellular carcinoma (HepG-2) and breast adenocarcinoma (MCF-7) cell lines. The plant species revealed different metabolomic profiling. C. oxyphyllus showed the highest phenolic and flavonoids contents compared to other plant extracts. While, among these plant extracts, T. polium showed the highest level of TAC, saponins and anthocyanins contents. C. oxyphyllus showed the highest inhibition concentration 50% (IC50) against HepG-2 (18.8 μg/ml) and MCF-7 (4.1 μg/ml). The high-performance liquid chromatography ‎analysis of C. oxyphyllus extract revealed the presence of high content of benzoic acid and vanillic acid (phenolics) along with hesperidin (flavonoids). In conclusion, among the screened plants, C. oxyphyllus has the most potent anticancer activity against HepG-2 and MCF-7 cell lines in vitro

Background: Cardiovascular diseases are among the most common causes of mortality worldwide. Therefore, it is necessary to control the risk factors of these patients. Since the level of inflammatory markers and lipid profiles has increased in cardiovascular diseases and due to the increasing role of plants in the treatment of diseases, the current study aimed to investigate the effect of hydroalcoholic extract of Teucrium polium on inflammatory markers and lipid profile in hypercholesterolemic rats. Materials and methods: A total of 24 adult male Wistar rats were randomly divided into four groups of six each and treated with oral administration for 8 weeks. The control group received normal diet, the sham group received high-cholesterol diet and experimental groups 1 and 2 received high-cholesterol diet in the 8 weeks and doses of 85 and 170 mg/kg, respectively, of the T. polium hydroalcoholic extract (TPHAE) in the second 4 weeks. At the beginning and the end of the study, rats were examined for biochemical parameters. The mean level of variables for each group was presented as mean +/- standard error of mean. Results: The results of this study showed that, after administration of TPHAE, there was a significant decrease in the mean of inflammatory markers in all groups compared to sham group (P

Purpose: Angiogenesis plays an important role in numerous pathophysiological events like cancer. As a result of this, tranilast as an anti-fibrotic drug induces the promising antitumor activities through the inhibition of angiogenesis. Further, Teucrium polium (TP) is a herbal medicine (family Lamaceae) with antitumor properties. This study was conducted to investigate the combination effects of tranilast and T. polium on human umbilical vein endothelial cells (HUVECs) viability and apoptotic genes expression. Methods: The HUVECs line was treated using different doses of tranilast and T. polium alone or their combination. The cell cytotoxicity was evaluated using MTT and LDH assays; apoptosis was examined using acridine orange/ethidium bromide staining, nitric oxide (NO) production was evaluated using Griess reaction and the expression of BAX and BCL-2 genes were detected using real-time RT-PCR. One-way analysis of variance (ANOVA) test was used to compare the data in different groups. Results: The survival rate of HUVECs was significantly reduced (p

The susceptibility of Teucrium polium essential oil (EO) as an antioxidant for canola oil was studied. Major compounds of the EO were 11-acetoxyeudesman-4-α-ol (26.3%) and α-bisabolol (24.6%). Different concentrations (200, 600 and 1200 ppm) of EO and synthetic antioxidant BHA (200 ppm) were added to canola oil and incubated for 60 days at room temperature. Acid value (AV), peroxide value (PV), carbonyl value (CV), iodine value (IV), total phenolics (TP), total polar compounds (TPC) and oxidative stability index (OSI) of canola oil were determined. Antioxidant capacity of the EO was measured by DPPH and β-carotene-linoleic acid assays. Results exhibited that DPPH and β-carotene-linoleic acid experiment detections on the EO were analogous in high concentrations to those detected on BHA. Moreover, incorporated EO samples had better AV, PV, CV, IV, TP and TPC than control. EO at concentration of 600 ppm indicated higher antioxidant activity in canola oil compared with BHA.

Methanolic extracts of 16 plant species, used in herbal and traditional medicinal preparations, collected from the same geographic region (Golcuk-Seben/Bolu, Turkey) were evaluated for their relative total phenolic contents, total antioxidant activities and their mineral contents in both dried plant samples and methanolic extracts. These species included Hypericum perforatum L., Thymus sipyleus Boiss., Equisetum arvense L., Achillea millefolium L., Tanacetum parthenium L., Thymus leucostomus Hausskn. & Velen., Usnea longissima , Herniaria incana Boiss., Teucrium polium L., Stachys bithynica Boiss., Anthemis nobilis L., Sambucus ebulus L., Berberis vulgaris L., Malus communis Desf., Matricaria chamomilla L . and Scorzonera laciniata L. Spectrophotometric Folin-Ciocalteu and DPPH free radical scavenging activity methods were performed to detect the total phenolic contents and total antioxidant activities of the methanolic extracts, respectively. The results show that the fruits of Berberis vulgaris L ., have the highest antioxidant activity and aerial parts of Tanacetum parthenium L. have the highest total phenolics (92.62%). Mineral contents were determined by inductively coupled – mass spectrometry (ICP-MS). All dried plant samples and methanolic extracts contained high amounts of Si, Cu, Fe and Ca. The highest levels of Si, Cu, Fe and Ca were found in Equisetum arvense L., Anthemis nobilis L . , and Thymus sipyleus Boiss. dried plant samples, whereas Se was only detected in Usnea longissima . The highest levels of Si, Cu, Fe and Ca were found in the methanolic extracts of Usnea longissima , Anthemis nobilis L. and Thymus sipyleus Boiss. respectively.

Plants reputed to have cancer-inhibiting potential and putative active components derived from those plants have emerged as an exciting new field in cancer study. Some of these compounds have cancer-inhibiting potential in different clinical staging levels, especially metastasis. A few of them which stabilize cell-cell adhesions are controversial topics. This review article introduces some effective herbal compounds that target E-cadherin/β-catenin protein complex. In this article, at first, we briefly review the structure and function of E-cadherin and β-catenin proteins, Wnt signaling pathway, and its target genes. Then, effective compounds of the Teucrium persicum, Teucrium polium, Allium sativum (garlic), Glycine max (soy), and Brassica oleracea (broccoli) plants, which influence stability and cellular localization of E-cadherin/β-catenin complex, were studied. Based on literature review, there are some compounds in these plants, including genistein of soy, sulforaphane of broccoli, organosulfur compounds of garlic, and the total extract of Teucrium genus that change the expression of variety of Wnt target genes such as MMPs, E-cadherin, p21, p53, c-myc, and cyclin D1. So they may induce cell-cycle arrest, apoptosis and/or inhibition of Epithelial-Mesenchymal Transition (EMT) and metastasis.

The antimicrobial effects of essential oil, ethanol and aqueous extracts of Teucrium polium L. were investigated against 13 microorganisms. Extracts and essential oil were obtained from maceration, decoction and hydrodistillation respectively. Samples were tested for their antimicrobial activity using the disk diffusion, the agar dilution and the agar incorporation method. Essential oil was analysed using GC/MS, results showed that β-pinene (35.97%) and α-pinene (13.32%) were the main components. Furthermore, essential oil exhibited the highest antimicrobial activity, it was most effective against Proteus mirabilis, Staphylococcus aureus and Citrobacter freundei where inhibition zone ranged between 15 and 25 mm, and with the microbial inhibitory concentration (MIC) values of 0.078–0.156 mg/ml. The oil and ethanol extract showed the best antifungal activity against Microsporum canis, Scopulariopsis brevicaulis, and Trichophyton rubrum with the inhibition percentage (I%) ranging from 18.94 to 100%. However, none of the samples exhibited antifungal activity against Aspergillus fumigatus. In this study, the obtained results showed significant effects of essential oils and ethanol extracts of T. polium which may used as a substitute to the synthetic drugs against certain microbial diseases.