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Teucrium polium Extract Enhances the Anti-Angiogenesis Effect of Tranilast on Human Umbilical Vein Endothelial Cells
- Source :
- Advanced Pharmaceutical Bulletin, Advanced Pharmaceutical Bulletin, Vol 8, Iss 1, Pp 131-139 (2018)
- Publication Year :
- 2018
- Publisher :
- Tabriz University of Medical Sciences, 2018.
-
Abstract
- Purpose: Angiogenesis plays an important role in numerous pathophysiological events like cancer. As a result of this, tranilast as an anti-fibrotic drug induces the promising antitumor activities through the inhibition of angiogenesis. Further, Teucrium polium (TP) is a herbal medicine (family Lamaceae) with antitumor properties. This study was conducted to investigate the combination effects of tranilast and T. polium on human umbilical vein endothelial cells (HUVECs) viability and apoptotic genes expression. Methods: The HUVECs line was treated using different doses of tranilast and T. polium alone or their combination. The cell cytotoxicity was evaluated using MTT and LDH assays; apoptosis was examined using acridine orange/ethidium bromide staining, nitric oxide (NO) production was evaluated using Griess reaction and the expression of BAX and BCL-2 genes were detected using real-time RT-PCR. One-way analysis of variance (ANOVA) test was used to compare the data in different groups. Results: The survival rate of HUVECs was significantly reduced (p
- Subjects :
- 0301 basic medicine
Angiogenesis
Tranilast
Pharmaceutical Science
Apoptosis
Teucrium polium
Pharmacology
Umbilical vein
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
food
medicine
Viability assay
General Pharmacology, Toxicology and Pharmaceutics
Chemistry
lcsh:RM1-950
Acridine orange
food.food
lcsh:Therapeutics. Pharmacology
030104 developmental biology
030220 oncology & carcinogenesis
Human Umbilical Vein Endothelial Cells (HUVECs)
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 22517308 and 22285881
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Advanced Pharmaceutical Bulletin
- Accession number :
- edsair.doi.dedup.....79c099f2670aa2786d71ff383789c7bc