1. Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
- Author
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Silvia Ghimenti, Christina Petersen, Tommaso Lomonaco, Francesca G. Bellagambi, Abdelhamid Errachid, Denise Biagini, Marie Hangouët, Aldo Paolicchi, Pietro Salvo, Roger Fuoco, Maria Franzini, Fabio Di Francesco, and Maria Giovanna Trivella
- Subjects
Male ,Saliva ,030204 cardiovascular system & hematology ,01 natural sciences ,0302 clinical medicine ,Natriuretic Peptide, Brain ,Natriuretic peptide ,80 and over ,Aged, 80 and over ,Multidisciplinary ,Protein Stability ,Brain ,Middle Aged ,Healthy Volunteers ,Chemistry ,Biomarker (medicine) ,Medicine ,Therapy monitoring ,Female ,N-terminal pro-Brain Natriuretic Peptide ,medicine.drug_class ,Science ,Cardiology ,Enzyme-Linked Immunosorbent Assay ,Article ,Specimen Handling ,Saliva analysis ,Aged ,Biomarkers ,Diagnostic Tests, Routine ,Heart Failure ,Humans ,Peptide Fragments ,03 medical and health sciences ,Diagnostic Tests ,Natriuretic Peptide ,medicine ,Routine ,Chromatography ,business.industry ,010401 analytical chemistry ,Gold standard (test) ,medicine.disease ,0104 chemical sciences ,Heart failure ,business - Abstract
Heart failure (HF) is the main cause of mortality worldwide, particularly in the elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) is the gold standard biomarker for HF diagnosis and therapy monitoring. It is determined in blood samples by the immunochemical methods generally adopted by most laboratories. Saliva analysis is a powerful tool for clinical applications, mainly due to its non-invasive and less risky sampling. This study describes a validated analytical procedure for NT-proBNP determination in saliva samples using a commercial Enzyme-Linked Immuno-Sorbent Assay. Linearity, matrix effect, sensitivity, recovery and assay-precision were evaluated. The analytical approach showed a linear behaviour of the signal throughout the concentrations tested, with a minimum detectable dose of 1 pg/mL, a satisfactory NT-proBNP recovery (95–110%), and acceptable precision (coefficient of variation ≤ 10%). Short-term (3 weeks) and long-term (5 months) stability of NT-proBNP in saliva samples under the storage conditions most frequently used in clinical laboratories (4, − 20, and − 80 °C) was also investigated and showed that the optimal storage conditions were at − 20 °C for up to 2.5 months. Finally, the method was tested for the determination of NT-proBNP in saliva samples collected from ten hospitalized acute HF patients. Preliminary results indicate a decrease in NT-proBNP in saliva from admission to discharge, thus suggesting that this procedure is an effective saliva-based point-of-care device for HF monitoring.
- Published
- 2021