1. Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells
- Author
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Shinsuke Uda, Jun Hamazaki, Izumi Ohigashi, Hiroyuki Kondo, Hidetaka Kosako, Keiji Tanaka, Shigeo Murata, Ryo Motosugi, Hiroyuki Kubota, Mina Kozai, Sayumi Fujimori, Victoria Hoffmann, Yu Tanaka, Amy Palin, Yousuke Takahama, Yosuke Matsushita, Toyomasa Katagiri, and Kenta Kondo
- Subjects
0301 basic medicine ,Proteomics ,Proteomic Profiling ,Protein subunit ,Cell Differentiation ,Epithelial Cells ,Thymus Gland ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Transcriptome ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,lcsh:Biology (General) ,Proteasome ,Humans ,lcsh:QH301-705.5 ,030217 neurology & neurosurgery ,Function (biology) - Abstract
SUMMARY The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology., In Brief Ohigashi et al. show that the use of cyclin D1-transgenic mice allows quantitative proteomic analysis of cortical and medullary thymic epithelial cells (TECs). Results provide a trans-omics platform for further exploration of TEC biology and reveal the specific impact of the thymoproteasome on proteasome subunit composition in cortical TECs., Graphical Abstract
- Published
- 2019